LOCUS       CP082640               13949 bp    DNA     circular BCT 14-JAN-2022
DEFINITION  Salmonella enterica subsp. enterica serovar Hadar strain CVM
            N17S1270 isolate 17CO10GT07S-B plasmid pN17S1270, complete sequence.
ACCESSION   CP082640
VERSION     CP082640.1
DBLINK      BioProject: PRJNA292661
            BioSample: SAMN20351463
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Hadar
  ORGANISM  Salmonella enterica subsp. enterica serovar Hadar
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 13949)
  AUTHORS   Li,C., Tyson,G.H., Hsu,C.H., Harrison,L., Strain,E., Tran,T.T.,
            Tillman,G.E., Dessai,U., McDermott,P.F. and Zhao,S.
  TITLE     Long-Read Sequencing Reveals Evolution and Acquisition of
            Antimicrobial Resistance and Virulence Genes in Salmonella enterica
  JOURNAL   Front Microbiol 12, 777817 (2021)
   PUBMED   34867920
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13949)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2020
            Assembly Method :: SMRTLink Microbial Assembly v. SMRTLink9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 791x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/31/2021 14:30:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,622
            CDSs (total)                      :: 4,497
            Genes (coding)                    :: 4,405
            CDSs (with protein)               :: 4,405
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 19
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 59 of 92
            Pseudo Genes (internal stop)      :: 18 of 92
            Pseudo Genes (multiple problems)  :: 26 of 92
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13949
                     /organism="Salmonella enterica subsp. enterica serovar
                     Hadar"
                     /mol_type="genomic DNA"
                     /strain="CVM N17S1270"
                     /serovar="Hadar"
                     /isolate="17CO10GT07S-B"
                     /isolation_source="ground turkey"
                     /sub_species="enterica"
                     /db_xref="taxon:149385"
                     /plasmid="pN17S1270"
                     /geo_loc_name="USA:CO"
                     /collection_date="2017-10"
                     /collected_by="CO"
     gene            1..3006
                     /locus_tag="KZZ26_23050"
     CDS             1..3006
                     /locus_tag="KZZ26_23050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="UAD42185.1"
                     /translation="MSVDFLTTEQVESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            complement(3071..3721)
                     /gene="tetR(A)"
                     /locus_tag="KZZ26_23055"
     CDS             complement(3071..3721)
                     /gene="tetR(A)"
                     /locus_tag="KZZ26_23055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="UAD42189.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            3827..5026
                     /gene="tet(A)"
                     /locus_tag="KZZ26_23060"
     ARG             3828..5023
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(5058..5942)
                     /locus_tag="KZZ26_23065"
     CDS             complement(5058..5942)
                     /locus_tag="KZZ26_23065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="UAD42187.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPA
                     GLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVV
                     FISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLT
                     RKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYF
                     LWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSN
                     RTPRARIACRKSP"
                     /name="EamA family transporter"
     gene            complement(6080..6487)
                     /locus_tag="KZZ26_23070"
     CDS             complement(6080..6487)
                     /locus_tag="KZZ26_23070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="UAD42188.1"
                     /translation="MRGRPVLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEG
                     LAIERGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYD
                     VRLVQDCCYDPDRDAHEALLRSGFGGRVQVV"
                     /name="cysteine hydrolase"
     gene            6476..8106
                     /locus_tag="KZZ26_23075"
                     /pseudo=""
     CDS             6476..8106
                     /locus_tag="KZZ26_23075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            8140..8745
                     /locus_tag="KZZ26_23080"
                     /pseudo=""
     CDS             8140..8745
                     /locus_tag="KZZ26_23080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            9567..9761
                     /locus_tag="KZZ26_23085"
     CDS             9567..9761
                     /locus_tag="KZZ26_23085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UAD42180.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            10415..10527
                     /locus_tag="KZZ26_23090"
                     /pseudo=""
     CDS             10415..10527
                     /locus_tag="KZZ26_23090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(10758..11054)
                     /locus_tag="KZZ26_23095"
     CDS             complement(10758..11054)
                     /locus_tag="KZZ26_23095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="UAD42181.1"
                     /translation="MINLVDPVHPGVFIREMFMEPFEISAADLSEKLHVSPSTLSRVLN
                     GKADLSVEMALRLSRVLGRSAESWINMQTQYSLNASRKSPNVIKELDELVPIF"
                     /name="HigA family addiction module antidote protein"
     gene            11399..11680
                     /locus_tag="KZZ26_23100"
     CDS             11399..11680
                     /locus_tag="KZZ26_23100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="UAD42182.1"
                     /translation="MIINIKHKGLRKFAETGNNAGIPQQFTQRLKLILTALSTATNLQD
                     MMDLKALRCHPLKGDRQGEYSLEVNGNWRVVFKLEEPNVFDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(12188..13048)
                     /locus_tag="KZZ26_23105"
     CDS             complement(12188..13048)
                     /locus_tag="KZZ26_23105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="UAD42183.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="class A broad-spectrum beta-lactamase TEM-1"
     gene            complement(13231..13788)
                     /locus_tag="KZZ26_23110"
     CDS             complement(13231..13788)
                     /locus_tag="KZZ26_23110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="UAD42184.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     ARG             complement(12192..13048)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(9053..9308)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="89.0"
                     /evalue="1.21e-116"
     Typing          complement(9433..9531)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          complement(9835..9917)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.79e-36"
     Typing          9930..10484
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.614"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atgtccgttg attttttgac cactgagcag gttgagagtt atggcaggtt cactggcgaa
       61 cccgatgaac ttcagctggc gcgttatttt catcttgatg aagcggataa agaatttatc
      121 gggaaaagcc ggggtgatca caatcgactt ggtattgccc tgcaaatcgg gtgtgtgcgt
      181 tttctgggca cttttcttac tgacatgaat catattcctt ccggcgtccg gcattttacc
      241 gccagacagc tcgggattcg tgatatcacc gttcttgcag aatacggtca gagggaaaat
      301 acccgccgtg agcatgcagc gctgatacgt cagcactatc agtatcgtga atttgcctgg
      361 ccctggacat ttcgccttac ccgtctttta tatacccgga gctggataag caacgaacgt
      421 cctggcctgc ttttcgacct ggcgacaggg tggcttatgc aacatcgtat tattctcccc
      481 ggagccacca cgctgacccg gttgatttca gaggtaaggg aaaaggcgac gttgcgcctg
      541 tggaacaaac tggcactgat accgtcagcc gaacagcgtt cacagctgga gatgctgctg
      601 gggccaactg attgcagccg cctgtcttta ctggaatcac tgaaaaaagg ccctgtgacc
      661 atcagtggtc cggcgtttaa tgaagcaatt gaacgctgga aaactctgaa cgattttggc
      721 ctgcatgctg aaaacctgag tacactcccg gctgtgcgcc tgaaaaatct cgcacgttat
      781 gctggtatga cttcggtgtt caatattgcc aggatgtcac cgcagaaaag gatggcggtt
      841 ctggttgcct ttgtccttgc atgggaaacg ctggcgctgg atgatgcact ggacgttctg
      901 gacgccatgc tggccgttat catccgtgac gccagaaaga ttgggcagaa aaaacggctc
      961 cgctcgctga aggatctgga taaatctgca ttggcgctcg ccagcgcatg ttcgtacttg
     1021 ctgaaagaag aaacaccgga cgaatcgatt cgtgctgagg tgttcagcta catccctagg
     1081 caaaagctgg ctgaaatcat cacgcttgtc cgtgaaattg cccggccctc agacgataat
     1141 tttcatgacg aaatggtgga gcagtacggg cgcgttcgtc gtttcctgcc ccatctgctg
     1201 aataccgtta aattttcatc cgcacctgcc ggggttacca ctctgaatgc ctgtgactac
     1261 ctcagccggg agttcagctc acggcggcag ttttttgacg acgcaccaac ggaaatcatc
     1321 agtcagtcat ggaaacggct ggtgattaac aaggaaaaac atatcacccg aaggggatac
     1381 acgctctgct ttctcagtaa actgcaggat agtctgagac ggagggatgt ctacgttacc
     1441 ggcagtaacc ggtggggaga tcctcgtgca agattactac agggtgctga ctggcaggca
     1501 aatcggatta aggtttatcg ttctttgggg cacccgacag acccgcagga agcaataaaa
     1561 tctctgggcc atcagcttga tagtcgttac agacaggttg ctgcacgtct tggcgaaaat
     1621 gaggctgtcg aactcgatgt ttctggcccg aagccccggt tgacaatttc tcccctcgcc
     1681 agtcttgatg agccggacag tctgaaacga ctgagcaaaa tgatcagtga tctgctccct
     1741 ccggtggatt taacggagtt gctgctcgaa attaacgccc ataccggatt tgctgatgag
     1801 tttttccatg ccagtgaagc cagtgccaga gttgatgatc tgcccgtcag catcagcgcc
     1861 gtgctgatgg ctgaagcctg caatatcggt ctggaaccac tgatcagatc aaatgttcct
     1921 gcactgaccc gacaccggct gaactggaca aaagcgaact atctgcgggc tgaaactatc
     1981 accagcgcta atgccagact ggttgatttt caggcaacgc tgccactggc acagatatgg
     2041 ggtggaggag aagtggcatc tgcagatgga atgcgctttg ttacgccagt cagaacaatc
     2101 aatgccggac cgaaccgcaa atactttggt aataacagag ggatcacctg gtacaacttt
     2161 gtgtccgatc agtattccgg ctttcatggc atcgttatac cggggacgct gagggactct
     2221 atctttgtgc tggaaggcct tctggaacag gagaccgggc tgaatccaac cgaaattatg
     2281 accgatacgg caggtgccag cgatcttgtc tttggccttt tctggctgct gggataccag
     2341 ttttctccac gcctggctga tgccggtgct tcggttttct ggcgaatgga ccatgatgcc
     2401 gactatggcg tgctgaatga tattgccaga gggcaatcag atccccgaaa aatagtcctt
     2461 cagtgggacg aaatgatccg gaccgcaggc tccctgaagc tgggcaaagt acaggcctca
     2521 gtgctggtcc gttcattgct gaaaagtgaa cgtccctccg gactgactca ggcaatcatt
     2581 gaagtggggc gcatcaacaa aacgctgtat ctgcttaatt atattgatga tgaagattac
     2641 cgccggcgca ttctgaccca gcttaatcgg ggagaaagcc gtcatgcagt tgccagagcc
     2701 atctgtcacg gtcaaaaagg tgagataaga aaacgatata ccgacggtca ggaagatcag
     2761 ttgggagctc tggggctggt cactaacgcc gtcgtgttat ggaacactat ttatatgcag
     2821 gcagctctgg atcatctccg ggcgcagggt gaaacactga atgatgaaga tatcgcacgc
     2881 ctctccccgc tttgccacgg acatatcaat atgctcggcc attattcctt cacgctggca
     2941 gaactggtga ccaaaggtca tctgagacca ttaaaagagg cgtcagaggc agaaaacgtt
     3001 gcttaacgtg agttttcgtt ccactgagcg tcagacccct gagtgggttg cgctccggca
     3061 gcggtcctga tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc
     3121 ttttcgccaa tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg
     3181 cttcgtcgaa ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg
     3241 tgccgccgcg ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag
     3301 tgaagtagct gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc
     3361 agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg
     3421 catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc
     3481 cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg
     3541 ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa
     3601 gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc
     3661 cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca
     3721 tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa
     3781 tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacag
     3841 acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat
     3901 gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta
     3961 tggcattctg ctggcgctgt atgcgttgat gcaatttgcc tgcgcacctg tgctgggcgc
     4021 gctgtcggat cgtttcgggc ggcggccggt cttgctcgtc tcgctggccg gcgctgctgt
     4081 cgactacgcc atcatggcga cggcgccttt cctttgggtt ctctatatcg ggcggatcgt
     4141 ggccggcatc accggggcga ctggggcggt agccggcgct tatattgccg atatcactga
     4201 tggcgatgag cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt tcgggatggt
     4261 cgcgggacct gtgctcggtg ggctgatggg cggtttctcc ccccacgctc cgttcttcgc
     4321 cgcggcagcc ttgaacggcc tcaatttcct gacgggctgt ttccttttgc cggagtcgca
     4381 caaaggcgaa cgccggccgt tacgccggga ggctctcaac ccgctcgctt cgttccggtg
     4441 ggcccggggc atgaccgtcg tcgccgccct gatggcggtc ttcttcatca tgcaacttgt
     4501 cggacaggtg ccggccgcgc tttgggtcat tttcggcgag gatcgctttc actgggacgc
     4561 gaccacgatc ggcatttcgc ttgccgcatt tggcattctg cattcactcg cccaggcaat
     4621 gatcaccggc cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat
     4681 tgccgacggc acaggctaca tcctgcttgc cttcgcgaca cggggatgga tggcgttccc
     4741 gatcatggtc ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag caatgttgtc
     4801 caggcaggtg gatgaggaac gtcaggggca gctgcaaggc tcactggcgg cgctcaccag
     4861 cctgacctcg atcgtcggac ccctcctctt cacggcgatc tatgcggctt ctataacaac
     4921 gtggaacggg tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct
     4981 gcgtcgcggg ctttggagcg gcgcagggca acgagccgat cgctgatcgt ggaaacgata
     5041 ggcctatgcc atgcgggtca aggcgacttc cggcaagcta tacgcgccct aggagtgcgg
     5101 ttggaacgtt ggcccagcca gatactcccg atcacgagca ggacgccgat gatttgaagc
     5161 gcactcagcg tctgatccaa gaacaaccat cctagcaaca cggcggtccc cgggctgaga
     5221 aagcccagta aggaaacaac tgtaggttcg agtcgcgaga tcccccggaa ccaaaggaag
     5281 taggttaaac ccgctccgat caggccgagc cacgccaggc cgagaacatt ggttcctgta
     5341 ggcatcggga ttggcggatc aaacactaaa gctactggaa cgagcagaag tcctccggcc
     5401 gccagttgcc aggcggtaaa ggtgagcaga ggcacgggag gttgccactt gcgggtcagc
     5461 acggttccga acgccatgga aaccgccccc gccaggcccg ctgcgacgcc gacaggatct
     5521 agcgctgcgt ttggtgtcaa caccaacagc gccacgcccg cagttccgca aatagccccc
     5581 aggaccgcca tcaatcgtat cgggctacct agcagagcgg cagagatgaa cacgaccatc
     5641 agcggctgca cagcgcctac cgtcgccgcg accccgcccg gcaggcggta gaccgaaata
     5701 aacaacaagc tccagaatag cgaaatatta agtgcgccga ggatgaagat gcgcatccac
     5761 cagattcccg ttggaatctg tcggacgatc atcacgagca ataaacccgc cggcaacgcc
     5821 cgcagcatcg cgaccgtcat cggtgagaag ttcggcaggt attgggtggt gacaatgtag
     5881 gtgctgcccc aaatggcagg tgctatcgct gtgaacaata aatcgggcgt gcgtaatgac
     5941 atgcgattac aagacctccg ctacggcgat gggtttttga gagttgcagg cgccgcgatg
     6001 accgcaccct gacttggact ggccctgcct cgcaccaatc cacctgcggc tatggccgcg
     6061 atcatgcgag gcccgaaaat tacacgacct gtacacgtcc gccgaacccg gaacgcaaca
     6121 gagcttcgtg ggcatcccga tccgggtcgt agcagcagtc ctggaccaaa cgcacgtcgt
     6181 agtccgcatc actagcccag gcgacgcttg aaagaacaac gccggtggtg cttatcccgg
     6241 ccatgacaag cgtgcttacg ccgcgcgtcc gaaggtcggc gtcgagcgcc gtgccataaa
     6301 agacgctggc tcgcggacat gcatagaaaa ggtcgccccg ttcgatcgca agcccttcga
     6361 cgggcagacc ggtgcgaaat cgtccgctcg ggagatacgg tgagatttgg cgattcgtcg
     6421 ccggcggcgc atgttcatac tcttcgccga gcgaaaagtt ggggaacagc acgggccggc
     6481 cgcgcaaggc gtgctggcag ccgtgcagac cctgcgtgag atgaacgccg acaacctgcg
     6541 caaggtgccg gccgatgcac ccacggcctt catcaagccg cgctggaagc cgctggtgat
     6601 caccccggaa ggcctcgacc ggaaattcta cgaaatctgc gccctgtccg agctgaagaa
     6661 cgccctgcgc tccggcgaca tctgggtcaa gggctcgcgg cagttccgcg acttcgacga
     6721 ctacctgctg ccggccgaga agttcgccgc actcaagcgc gagcaggccc tgcccctggc
     6781 gatcaacccg aacagcgacc agtacctgga agagcgtttg cagctgctgg acgagcagtt
     6841 ggccaccgtc acccgcctgg ccaaggacaa cgagctgccc gatgccatcc tcaccgagtc
     6901 agggctgaaa atcaccccgc tggatgcggc ggtgccggat cgggcgcagg cgctgatcga
     6961 ccaaaccagc cagttactgc cgcgcatcaa gatcaccgaa ctgctgatgg acgtggacga
     7021 ctggacgggc ttcagccgcc acttcaccca cttgaaggac ggggccgagg ccaaagacag
     7081 gacgttgctg ctgtccgcaa tcctcggtga tgcgatcaac ctcgggctga ccaagatggc
     7141 cgagtcgagc cccggcctga cctacgccaa gctgtcctgg ctgcaagcct ggcacatccg
     7201 cgacgaaacc tattcggcgg ccttggccga gctggtcaac caccagtatc gccacgcctt
     7261 tgccgcccac tggggcgacg gcacgacctc atcctccgat ggccagcgct tccgcgcggg
     7321 tggccggggc gagagcaccg ggcacgtcaa cccgaagtac ggtagcgagc cgggacggct
     7381 gttctatacc catatctccg accagtacgc gccgttcagc acccgcgtgg tgaatgtcgg
     7441 cgtccgcgat tccacctatg tgctcgacgg cctgctgtac cacgagtccg acctgcggat
     7501 cgaggagcac tacaccgaca cggccggctt caccgatcac gtctttgccc tgatgcacct
     7561 gctaggcttc cgcttcgcgc cgcgcatccg cgacctcggc gaaaccaagc tgtacgtgcc
     7621 gcagggcgtg caagcctacc cgacgttgcg cccgctgatc ggcggcaccc tgaacatcaa
     7681 gcacgtgcgt gcccactggg ccggatcgag cgcacgctgt tcatcctgga ctggctgcaa
     7741 agtgttgaac tgcgccgccg cgtgcatgcc ggcctgaaca aaggtgaggc gcgcaactcg
     7801 ctggccaggg cggtgttctt caaccgcctt ggggaaatca gggatcggag cttcgagcag
     7861 cagcgctacc gggccagcgg cctcaacctg gtgacggcgg ctatcgtgct gtggaacacg
     7921 gtgtacctgg aacgcgccac ccaggggttg gtcgaggccg gcaagccggt ggacggcgag
     7981 ctgctgcaat tcctgtcgcc gctgggctgg gagcacatca acctaaccgg cgattacgtc
     8041 tggcggcaga gccgcagact ggaagacggg aagtttcggc ccttacggat gcccggaaaa
     8101 ccttagcgta cgattttttc cgaattctgc gggctccccg actatggcgt gctgaatgat
     8161 attgccagag ggcaatcaga tccccgaaaa atagtccttc agtgggacga aatgatccgg
     8221 accgctggct ccctgaagct gggcaaagta caggtttcag tgctggtccg ttcattgctg
     8281 aaaagtgaac gtccttccgg actgactcag gcaatcattg aagtggggcg catcaacaaa
     8341 acgctgtatc tgcttaatta tattgatgat gaagattacc gccggcgcat tctgacccag
     8401 cttaatcggg gagaaagtcg ccatgccgtt gccagagcca tctgtcacgg tcaaaaaggt
     8461 gagataagaa aacgatatac cgacggtcag gaagatcaac tgggcacact ggggctggtc
     8521 actaacgccg tcgtgttatg gaacactatt tatatgcagg cagccctgga tcatctccgg
     8581 gcgcagggtg aaacactgaa tgatgaagat atcgcacgcc tctccccgct ttgccacgga
     8641 catatcaata tgctcggcca ttattccttc acgctggcag aactggtgac caaaggacat
     8701 ctgagaccat taaaagaggc gtcagaggca gaaaacgttg cttaacgtga gttttcgttc
     8761 cactgagcgt cagaccccta aaaccatccc gtaaatcaca gcatttacca taacgtcgca
     8821 ttacgcgcac cgctaaaacg cgctgaacgc gattctgccg cgaacaccgc aaaaagagat
     8881 gttttaccaa gcgaaaaccg gaaaaatgcg tcaggatcgc tttcagctca ctgcatagct
     8941 atgcatgaaa gtgaatggcg atcggtttgg gcgcatacgg tgcccatgag acacattttc
     9001 ggacattttc agttcgtgaa cccctgactc attgctacat atctgaacag acacccctag
     9061 cagcgccccc aggggcgtcc tgtaaaaaaa cacaccgcgc cgctagcagc acccctaata
     9121 taaaatgatg ttttttatga aaatagtcag taccacccct aaaaagcggt gttggcgcag
     9181 ctacaacacc gcgccgacac cgctttttta aatatcacaa agagagcaag aagaactaat
     9241 ttgtcataac tctctattta taaagaaaaa tcagcaaaaa attgtttttg cgtggggtgt
     9301 gggcatttca ggcatgagaa cagactcgcg tttttccgga gtatcagcgg aattattgat
     9361 taagaaacac tcgacgcaga gcaaaatttt atgcaacagc ggctgcactg cacagggatt
     9421 atttatcagt tgatatgctg tgccatgata cgtcctaaaa aaacaggaca ggatgtgcaa
     9481 ttgtaatacc gtcgcccgca acgtcttaca attaccacct ggtcagggtc cacccgacac
     9541 ccggtactga cggagaacaa cggaaggtga acaaacagca acaaactgcg ctgaatatgg
     9601 cgcgatttat caaaagccag agcctgacac tgcttgaaaa actggatgct ctggatgccg
     9661 acgaacaggc ggccatgtgc gaacgactgc acgaactcgc ggaagaactc cagaacagca
     9721 tccaggttcg ctttgagacc gaaagtgaaa caggaacata acgaagctga cggaggcgct
     9781 cacggcttgt ccgcgagcgt tttcggcagc agacaagccc gccagggcgc gtcagcgggt
     9841 gtcggggcga agccctgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     9901 tgcggcatca gtgcagattg tatgaaaagt gccccatgcc gggtgtgaaa ttccgcacag
     9961 atgcgtaagg agaaaatgcg cgtccaggcg cttttccgct tcctcgctca ctgactcgct
    10021 acgctcggtc gttcggctgc ggcgagcggt actgacttac acaaaaacgg cagcacggtt
    10081 atccacagaa tcaggggata aggccggaaa gaacatgtga gcaaaagacc aagaacagga
    10141 agaaggccgc gccgcaggcg tttttccata ggctccgccc ccctgacgag catcacagaa
    10201 atcgacgctc aagtcagagg tggcgaaacc cgacaggact taaagatacc agacgtttcc
    10261 ccctggtggc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    10321 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcgg
    10381 ttcagtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    10441 ccgctgcgcc ttatccggta actatcatct tgagtccaac ccggaaagac atgcaaaaca
    10501 ccattggcag cagccactgg taactgattt agaggagtta gtcttgaagt tatgcgccag
    10561 ttaaggctaa actgaaagaa caagttttgg tgactgcgct cctccaagcc agttaccctg
    10621 gttcagaaac ttgccggtta ctgaaccttc gaaaaaacca ccttgccggg tggttttttc
    10681 gtttacgaga aacgatacga tccagttgcc tgagagtcaa gacagaaaaa ggccgcatat
    10741 agcggccttt tcttttctca aaatatagga accaattcat ctaattcctt aatgacatta
    10801 ggtgacttcc tgctcgcgtt aagactgtat tgagtctgca tgtttatcca cgactcggca
    10861 ctgcgaccaa gaactcgaga aagccttaat gccatctcaa cacttaaatc agcttttcca
    10921 ttcagcactc tacttagagt ggaaggtgat acatgcagtt tttcagacag gtccgcggct
    10981 gatatttcaa acggttccat aaacatctca cggataaaaa cccctggatg tactggatcg
    11041 acaagattaa tcataccttc ctctgaagcc tatattagtt ttaaaaagat aggcctattg
    11101 cgttttcaga ttgcgcattg cgcataacgc aactcattgt tgactaaaaa aaagaatgac
    11161 attatgatgc acattggtgc gtctaatcat tccttatgat gtgggtactt gatgtgaaat
    11221 tcaaggcccc ggcattgtac agcgcatcaa accaatgtca acagattaaa atgcgacttg
    11281 gttaagtaaa acctacgtca tttattcaaa tagagcaagg ggtttgcaat ggaaatacgg
    11341 atgaaccgga aaaataaact gcgatgtttc tatctaaaca aatatgggat ggtgattaat
    11401 gatcataaat atcaagcata aaggtttgcg taagtttgcc gaaacaggta acaacgcagg
    11461 aattccgcaa cagtttactc aacgtctgaa gctgatccta acagccttaa gcacagcgac
    11521 gaacttgcag gatatgatgg atttaaaggc attgcgctgc catccattga agggagaccg
    11581 tcaaggagag tattcgttag aggtcaatgg aaactggcgc gtagtgttta agctcgaaga
    11641 acctaacgtc tttgacgtgg atctcgagga ttatcactaa gaaggtcctt aaagcatcaa
    11701 agtgacagta tcgatgcttt agggtctcat aaaaaagcaa aacctcgggg taagttatat
    11761 gaagtatttt ctgtaccaga tagtccctat agcagccatc atttgcgcag caattctagc
    11821 gctaaaaggt atcaatggct gggggtggtt cttaactgtt gggttcctgc ttgcaggggc
    11881 agatacagta aaggctcagt tatctaagta gtaataaaca ctatcaatag tggttggata
    11941 attgccaaaa aagcctctat ctgttctgaa acagacaaca aggaaaaatc ggtgtaagcc
    12001 acctccataa gaggtggtat tgatatcaac atagataaaa ggggtctgac gctcagtgga
    12061 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    12121 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    12181 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    12241 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    12301 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    12361 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    12421 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    12481 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
    12541 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    12601 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gcagcagtgt
    12661 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    12721 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    12781 cgagttgctc ttgcccggcg tcaacacggg ataataccgc accacatagc agaactttaa
    12841 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    12901 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    12961 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    13021 gggcgacacg aaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    13081 accagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    13141 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
    13201 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaatttt
    13261 ataaaccgtg gagcgggcaa tactgagctg atgagcaatt tccgttgcac cagtgccctt
    13321 ctgatgaagc gtcagcacga cgttcctgtc cacggtacgc ctgcggccaa atttgattcc
    13381 tttcagcttt gcttcctgtc ggccctcatt cgtgcgttct aggatcctcc ggcgttcagc
    13441 ctgtgccaca gccgacagga tggtgaccac catttgcccc atatcaccgt cggtactgat
    13501 cccgtcatca atgaaccgga ctgccacgcc ctgagcgtca aattccttta tcagttggat
    13561 catatcggca gtgtcgcggc caagacggtc gagcttctta accagaatga catcaccttc
    13621 ctccaccttc atcctcagca aatccagccc ttcccggtct gttgaactgc cggatgcctt
    13681 atcggtaaat atacggtttg ctttcacacc tgcgtctttg agtgctctga cctgaagatc
    13741 aagagactgc tgactggttg agacccgagc gtaaccaaaa agtcgcataa aaatgtacct
    13801 taaatcgaat atcggacaac tcatgtctat tattacaaat ttacgattta atagacatat
    13861 taatgtaaca gttttacgat gtccgataat ttataatatt tcgaacggtt gcagttgtgt
    13921 taaaaaagcc gtcaggcagg gaggccgat
//
