LOCUS       CP082638                9473 bp    DNA     circular BCT 14-JAN-2022
DEFINITION  Salmonella enterica subsp. enterica serovar Schwarzengrund strain
            CVM N17S1304 isolate 17GA11GT12-S2 plasmid pN17S1304-2, complete
            sequence.
ACCESSION   CP082638
VERSION     CP082638.1
DBLINK      BioProject: PRJNA292661
            BioSample: SAMN20351464
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Schwarzengrund
  ORGANISM  Salmonella enterica subsp. enterica serovar Schwarzengrund
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Li,C., Tyson,G.H., Hsu,C.H., Harrison,L., Strain,E., Tran,T.T.,
            Tillman,G.E., Dessai,U., McDermott,P.F. and Zhao,S.
  TITLE     Long-Read Sequencing Reveals Evolution and Acquisition of
            Antimicrobial Resistance and Virulence Genes in Salmonella enterica
  JOURNAL   Front Microbiol 12, 777817 (2021)
   PUBMED   34867920
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2020
            Assembly Method :: SMRTLink Microbial Assembly v. SMRTLink9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 546x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/31/2021 15:59:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,741
            CDSs (total)                      :: 4,621
            Genes (coding)                    :: 4,530
            CDSs (with protein)               :: 4,530
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 16 of 91
            Pseudo Genes (multiple problems)  :: 22 of 91
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9473
                     /organism="Salmonella enterica subsp. enterica serovar
                     Schwarzengrund"
                     /mol_type="genomic DNA"
                     /strain="CVM N17S1304"
                     /serovar="Schwarzengrund"
                     /isolate="17GA11GT12-S2"
                     /isolation_source="ground turkey"
                     /sub_species="enterica"
                     /db_xref="taxon:340190"
                     /plasmid="pN17S1304-2"
                     /geo_loc_name="USA:GA"
                     /collection_date="2017-11"
                     /collected_by="GA"
     gene            complement(join(7660..9473,1..316))
                     /locus_tag="KZZ32_23655"
     CDS             complement(join(7660..9473,1..316))
                     /locus_tag="KZZ32_23655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UAD46715.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHG
                     LVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEY
                     DADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQI
                     EQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQK
                     LASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPA
                     PRQRGMDRGGPDFSM"
                     /name="MobA/MobL family protein"
     gene            515..799
                     /locus_tag="KZZ32_23660"
     CDS             515..799
                     /locus_tag="KZZ32_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="UAD46716.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            1387..1461
                     /locus_tag="KZZ32_23665"
                     /pseudo=""
     CDS             1387..1461
                     /locus_tag="KZZ32_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /name="EamA family transporter"
     gene            complement(1493..2692)
                     /gene="tet(A)"
                     /locus_tag="KZZ32_23670"
     ARG             complement(1497..2692)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2798..3424
                     /gene="tetR(A)"
                     /locus_tag="KZZ32_23675"
     CDS             2798..3424
                     /gene="tetR(A)"
                     /locus_tag="KZZ32_23675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="UAD46718.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGGTVEQAPLSPLL
                     RAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(3592..4287)
                     /locus_tag="KZZ32_23680"
                     /pseudo=""
     CDS             complement(3592..4287)
                     /locus_tag="KZZ32_23680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /name="APH(3'') family aminoglycoside O-phosphotransferase"
     gene            complement(4348..5163)
                     /gene="sul2"
                     /locus_tag="KZZ32_23685"
     ARG             complement(4352..5163)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(5472..6323)
                     /locus_tag="KZZ32_23690"
     CDS             complement(5472..6323)
                     /locus_tag="KZZ32_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="UAD46711.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="replication protein"
     gene            complement(6310..7149)
                     /locus_tag="KZZ32_23695"
     CDS             complement(6310..7149)
                     /locus_tag="KZZ32_23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="UAD46712.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            complement(7179..7385)
                     /locus_tag="KZZ32_23700"
     CDS             complement(7179..7385)
                     /locus_tag="KZZ32_23700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAD46713.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="hypothetical protein"
     gene            complement(7387..7599)
                     /locus_tag="KZZ32_23705"
     CDS             complement(7387..7599)
                     /locus_tag="KZZ32_23705"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAD46714.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     ARG             complement(3563..4287)
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.07"
                     /coverage="90.26"
     Typing          complement(406..561)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          complement(6311..7132)
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(7664..9471)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gcaggcgctc ggcaccggtc aggtgctggg cgaactcgga cgccagcgcc ttctgctggt
       61 cgagggtcag ctcgaccggc agggcaaatt cgacctcctt gaacagccgc ccattggcgc
      121 gttcatacag gtcggcagca tcccagtagt cggcgggccg ctcgacgaac tccggcatgt
      181 gcccggattc ggcgtgcaag acttcatcca tgtcgcgggc atacttgcct tcgcgctgga
      241 tgtagtcggc cttggccctg gccgattggc cgcccgacct gctgccggtt ttcgccgtaa
      301 ggtgataaat cgccatgctg cctcgctgtt gcttttgctt ttcggctcca tgcaatggcc
      361 ctcggagagc gcaccgcccg aagggtggcc gttaggccag tttctcgaag agaaaccggt
      421 aagtgcgccc tcccctacaa agtagggtcg ggattgccgc cgctgtgcct ccatgatagc
      481 ctacgagaca gcacattaac aatggggtgt caagatggtt aaggggagca acaaggcggc
      541 ggatcggctg gccaagctcg aagaacaacg agcgcgaatc aatgccgaaa ttcagcgggt
      601 gcgggcaagg gaacagcagc aagagcgcaa gaacgaaaca aggcgcaagg tgctggtggg
      661 ggccatgatt ttggccaagg tgaacagcag cgagtggccg gaggatcggc tcatggcggc
      721 aatggatgcg taccttgaac gcgaccacga ccgcgccttg ttcggtctgc cgccacgcca
      781 gaaggatgag ccgggctgaa tgatcgaccg agacaggccc tgcggggctg cacacgcgcc
      841 cccacccttc gggtaggggg aaaggccgct aaagcggcta aaagcgctcc agcgtatttc
      901 tgcggggttt ggtgtggggt ttagcgggct ttgcccgcct ttccccctgc cgcgcagcgg
      961 tggggcggtg tgtagcctag cgcagcgaat agaccagcta tccggcctct ggccgggcat
     1021 attgggcaag ggcagcagcg ccccacaagg gcgctgataa ccgcgcctag tggattattc
     1081 ttagataatc atggatggat ttttccaaca ccccgccagc ccccgcccct gctgggtttg
     1141 caggtttggg ggcgtgacag ttattgcagg ggttcgtgac agttattgca ggggggcgtg
     1201 acagttattg caggggttcg tgacagttag tacgggagtg acgggcactg gctggcaatg
     1261 tctagcaacg gcaggcattt cggctgaggg taaaagaact ttccgctaag cgatagactg
     1321 tatgtaaaca cagtattgca aggacgcgga acatgcctca tgtggcggcc aggacggcca
     1381 gccgggatcg ggagtatctg gctgggccaa cgttccaacc gcactcctag ggcgcgtata
     1441 gcttgccgga agtcgccttg acccgcatgg cataggccta tcgtttccac gatcagcgat
     1501 cggctcgttg ccctgcgccg ctccaaagcc cgcgacgcag cgccggcagg cagagcaagt
     1561 agagggcagc gcctgcaatc catgcccacc cgttccacgt tgttatagaa gccgcataga
     1621 tcgccgtgaa gaggaggggt ccgacgatcg aggtcaggct ggtgagcgcc gccagtgagc
     1681 cttgcagctg cccctgacgt tcctcatcca cctgcctgga caacattgct tgcagcgccg
     1741 gcattccgat gccacccgaa gcaagcagga ccatgatcgg gaacgccatc catccccgtg
     1801 tcgcgaaggc aagcaggatg tagcctgtgc cgtcggcaat cattccgagc atgagtgccc
     1861 gcctttcgcc gagccgggcg gctacagggc cggtgatcat tgcctgggcg agtgaatgca
     1921 gaatgccaaa tgcggcaagc gaaatgccga tcgtggtcgc gtcccagtga aagcgatcct
     1981 cgccgaaaat gacccaaagc gcggccggca cctgtccgac aagttgcatg atgaagaaga
     2041 ccgccatcag ggcggcgacg acggtcatgc cccgggccca ccggaacgaa gcgagcgggt
     2101 tgagagcctc ccggcgtaac ggccggcgtt cgcctttgtg cgactccggc aaaaggaaac
     2161 agcccgtcag gaaattgagg ccgttcaagg ctgccgcggc gaagaacgga gcgtgggggg
     2221 agaaaccgcc catcagccca ccgagcacag gtcccgcgac catcccgaac ccgaaacagg
     2281 cgctcatgaa gccgaagtgc cgcgcgcgct catcgccatc agtgatatcg gcaatataag
     2341 cgccggctac cgccccagtc gccccggtga tgccggccac gatccgcccg atatagagaa
     2401 cccaaaggaa aggcgccgtc gccatgatgg cgtagtcgac agcagcgccg gccagcgaga
     2461 cgagcaagac cggccgccgc ccgaaacgat ccgacagcgc gcccagcaca ggtgcgcagg
     2521 caaattgcat caacgcatac agcgccagca gaatgccata gtgggcggtg acgtcgttcg
     2581 agtgaaccag atcgcgcagg aggcccggca gcaccggcat aatcaggccg atgccgacag
     2641 cgtcgagcgc gacagtgctc agaattacga tcaggggtct gttgggtttc acgtctggcc
     2701 tccggaccag cctccgctgg tccgattgaa cgcgcggatt ctttatcact gataagttgg
     2761 tggacatatt atgtttatca gtgataaagt gtcaagcatg acaaagttgc agccgaatac
     2821 agtgatccgt gccgccctgg acctgttgaa cgaggtcggc gtagacggtc tgacgacacg
     2881 caaactggcg gaacggttgg gggttcagca gccggcgctt tactggcact tcaggaacaa
     2941 gcgggcgctg ctcgacgcac tggccgaagc catgctggcg gagaatcata cgcattcggt
     3001 gccgagagcc gacgacgact ggcgctcatt tctgatcggg aatgcccgca gcttcaggca
     3061 ggcgctgctc gcctaccgcg atggcgcgcg catccatgcc ggcacgcgac cgggcgcacc
     3121 gcagatggaa acggccgacg cgcagcttcg cttcctctgc gaggcgggtt tttcggccgg
     3181 ggacgccgtc aatgcgctga tgacaatcag ctacttcact gttggggccg tgcttgagga
     3241 gcaggccggc ggcaccgttg aacaggctcc gctctcgccg ctgttgcggg ccgcgataga
     3301 cgccttcgac gaagccggtc cggacgcagc gttcgagcag ggactcgcgg tgattgtcga
     3361 tggattggcg aaaaggaggc tcgttgtcag gaacgttgaa ggaccgagaa agggtgacga
     3421 ttgatcagga ccgctgccgg agcgcaaccc actcactaca gcagagccat gtagacaaca
     3481 tcccctcccc ctttccaccg cgtcagacgc ccgtagcagc ccgctacggg ctttttcatg
     3541 ccctgcccta gcgtccaagc ctcacggccg cgctcggcct ctctggcggc cttctggcgc
     3601 tgcccagttc tcttcggcgt tagcaatcat gagtgccaaa tcggcatagc gatctgctgt
     3661 tccgagccgc ccaaggtcga tcagacccgt gcattgaaga gttttagggt ccaccatgaa
     3721 gttcggcatg cagggatcac catggcaaac aaccatatcg gtgcgctctt ggtcgagccg
     3781 caccggtagc tctcgttcga cacgagccaa aagatcgagc tgcggcgtac tcttgtcctc
     3841 gtccggtaag aagtcgggat tgacggcatt gcgggacacc acatcaacgg cgcgtccgaa
     3901 cattcgcgac agcctgcgct caaacggaca ttgatcaacc gataggctgt gaacagcgcc
     3961 aagttgctgc cccattgacg gccacgcttt gagcaaatcc gctccagaca gatcagccgc
     4021 cggtactccc ggaattgccg ttatcaccaa gcatgcaccc tcctgttcct cctgccagtt
     4081 gatgacctcg gggcaagcca cacctcgacc tttgagccaa atgaggcggt cacgctctcc
     4141 agcgagctca ccgcggcggg aagcaggtgc gattttcgcg aaggcatgcc cgtcaccacg
     4201 tcgaaaaaca aaatcaccag attctccgcc tctgacaggc aaccagtcag aatgcgattc
     4261 accaaaaaaa atattagttc gattcaatgg aggttccttc agttttctga tgaagcgcga
     4321 atatagagaa atatcccgaa tgtgcagtta acgaattctt gcggtttctt tcagcgccgc
     4381 caataccgcc agcccgtcgc gcaaggggcg cggctcgtgt gtgcggatga agtcagctcc
     4441 acctgcggcg gcggcaagct ctgcagcgag tgtcgcggcc ccgacatccc ccggaccacg
     4501 gcctgtgagc gcgcgcagaa aggatttgcg cgaaacagac agaagcaccg gcaaatcgaa
     4561 gcgcagccgc aattcatcga accgcgccag caccgagagc gaggtttcgg gagcagcccc
     4621 cagaaaaaac cccatgccgg gatcaaggac aaggcggttg cgtttgatac cggcacccgt
     4681 cagcgccgcg atgcgcgcgt caaagaacgc cgcaatgtga tccatgatgt cgccagcggg
     4741 tgcctcgcgc cgatctgcct gcccgtcttg caccgaatgc ataacgacga gtttggcaga
     4801 tgatttcgcc aattgcggat agaacgcagc gtctggaaaa ccgcgaatat cattgagata
     4861 ggccacacca cgcgacaagg cataggcttg cgtcgcgggt tgataactgt cgagcgagac
     4921 gggaatgcca tctgccttga gcgcgtccag caccggcgcg atacgctcga tttctgtgtc
     4981 ggacgaaaca ggcgcggcgt cggggttgct ggatgccgga ccgaggtcga tcacatctgc
     5041 cccctcggcc atcagcttac gcgcctgcgc aatggctgcg tctggcgcca gataccggcc
     5101 tccatcggag aaactgtccg aggttatgtt gacgatgccg aaaatgatga gcgatttatt
     5161 catgggggct tctataataa taataatcga gcatgagtct catacggatg ctcgggtcga
     5221 aagggaatcc ccaggcgagt aacctgtttg cggtgatcca ttagctgcag gagcagaaga
     5281 gcatacatct ggaagcaaag ccaggaaagc ggcctatgga gctgtgcggc agcgctcagt
     5341 aggcaatttt tcaaaatatt gttaagcctt ttctgagcat ggtatttttc atggtattac
     5401 caattagcag gaaaataagc cattgaatat aaaagataaa aatgtcttgt ttacaataga
     5461 gtgggggggg gtcagcctgc cgccttgggc cgggtgatgt cgtacttgcc cgccgcgaac
     5521 tcggttaccg tccagcccag cgcgaccagc tccggcaacg cctcgcgcac ccgctggcgg
     5581 cgcttgcgca tggtcgaacc actggcctct gacggccaga catagccgca caaggtatct
     5641 atggaagcct tgccggtttt gccggggtcg atccagccac acagccgctg gtgcagcagg
     5701 cgggcggttt cgctgtccag cgcccgcacc tcgtccatgc tgatgcgcac atgctggccg
     5761 ccacccatga cggcctgcgc gatcaagggg ttcagggcca cgtacaggcg cccgtccgcc
     5821 tcgtcgctgg cgtactccga cagcagccga aacccctgcc gcttgcggcc attctgggcg
     5881 atgatggata ccttccaaag gcgctcgatg cagtcctgta tgtgcttgag cgccccacca
     5941 ctatcgacct ctgccccgat ttcctttgcc agcgcccgat agctaccttt gaccaccatg
     6001 gcatcagcgg tgacggcctc ccacttgggt tccaggaaca gccggagctg ccgtccgcct
     6061 tcggtcttgg gttccgggcc aagcactagg ccattaggcc cagccatggc caccagccct
     6121 tgcaggatgc gcagatcatc agcgcccagc ggctccgggc cgctgaactc gatccgcttg
     6181 ccgtcgccgt agtcatacgt cacgtccagc ttgctgcgct tgcgctcgcc ccgcttgagg
     6241 gcacggaaca ggccgggggc cagacagtgc gccgggtcgt gccggacgtg gctgaggctg
     6301 tgcttgttct taggcttcac cacggggcac ccccttgctc ttgcgctgcc tctccagcac
     6361 ggcgggcttg agcaccccgc cgtcatgccg cctgaaccac cgatcagcga acggtgcgcc
     6421 atagttggcc ttgctcacac cgaagcggac gaagaaccgg cgctggtcgt cgtccacacc
     6481 ccattcctcg gcctcggcgc tggtcatgct cgacaggtag gactgccagc ggatgttatc
     6541 gaccagtacc gagctgcccc ggctggcctg ctgctggtcg cctgcgccca tcatggccgc
     6601 gcccttgctg gcatggtgca ggaacacgat agagcacccg gtatcggcgg cgatggcctc
     6661 catgcgaccg atgacctggg ccatggggcc gctggcgttt tcttcctcga tgtggaaccg
     6721 gcgcagcgtg tccagcacca tcaggcggcg gccctcggcg gcgcgcttga ggccgtcgaa
     6781 ccactccggg gccatgatgt tgggcaggct gccgatcagc ggctggatca gcaggccgtc
     6841 agccacggct tgccgttcct cggcgctgag gtgcgcccca agggcgtgca ggcggtgatg
     6901 aatggcggtg ggcgggtctt cggcgggcag gtagatcacc gggccggtgg gcagttcgcc
     6961 cacctccagc agatccggcc cgcctgcaat ctgtgcggcc agttgcaggg ccagcatgga
     7021 tttaccggca ccaccgggcg acaccagcgc cccgaccgta ccggccacca tgttgggcaa
     7081 aacgtagtcc agcggtggcg gcgctgctgc gaacgcctcc agaatattga taggcttatg
     7141 ggtagccatt gattgcctcc tttgcaggca gttggtggtt aggcgctggc ggggtcacta
     7201 cccccgccct gcgccgctct gagttcttcc aggcactcgc gcagcgcctc gtattcgtcg
     7261 tcggtcagcc agaacttgcg ctgacgcatc cctttggcct tcatgcgctc ggcatatcgc
     7321 gcttggcgta cagcgtcagg gctggccagc aggtcgccgg tctgcttgtc cttttggtct
     7381 ttcatatcag tcaccgagaa acttgccggg gccgaaaggc ttgtcttcgc ggaacaagga
     7441 caaggtgcag ccgtcaaggt taaggctggc catatcagcg actgaaaagc ggccagcctc
     7501 ggccttgttt gacgtataac caaagccacc gggcaaccaa tagcccttgt cacttttgat
     7561 caggtagacc gaccctgaag cgcttttttc gtattccata aaaccccctt ctgtgcgtga
     7621 gtactcatag tataacaggc gtgagtacca acgcaagcac tacatgctga aatctggccc
     7681 gcccctgtcc atgcctcgct ggcggggtgc cggtgcccgt gccagctcgg cccgcgcaag
     7741 ctggacgctg ggcagaccca tgaccttgct gacggtgcgc tcgatgtaat ccgcttcgtg
     7801 gccgggcttg cgctctgcca gcgctgggct ggcctcggcc atggccttgc cgatttcctc
     7861 ggcactgcgg ccccggctgg ccagcttctg cgcggcgata aagtcgcact tgctgaggtc
     7921 atcaccgaag cgcttgacca gcccggccat ctcgctgcgg tactcgtcca gcgccgtgcg
     7981 ccggtggcgg ctaagctgcc gctcgggcag ttcgaggctg gccagcctgc gggccttctc
     8041 ctgctgccgc tgggcctgct cgatctgctg gccagcctgc tgcaccagcg ccgggccagc
     8101 ggtggcggtc ttgcccttgg attcacgcag cagcacccac ggctgataac cggcgcgggt
     8161 ggtgtgcttg tccttgcggt tggtgaagcc cgccaagcgg ccatagtggc ggctgtcggc
     8221 gctggccggg tcggcgtcgt actcgctggc cagcgtccgg gcaatctgcc cccgaagttc
     8281 accgcctgcg gcgtcggcca ccttgaccca tgcctgatag ttcttcgggc tggtttccac
     8341 taccagggca ggctcccggc cctcggcttt catgtcatcc aggtcaaact cgctgaggtc
     8401 gtccaccagc accagaccat gccgctcctg ctcggcgggc ctgatataca cgtcattgcc
     8461 ctgggcattc atccgcttga gccatggcgt gttctggagc acttcggcgg ctgaccattc
     8521 ccggttcatc atctggccgg tggtggcgtc cctgacgccg atatcgaagc gctcacagcc
     8581 catggccttg agctgtcggc ctatggcctg caaagtcctg tcgttcttca tcgggccacc
     8641 aagcgcagcc agatcgagcc gtcctcggtt gtcagtggcg tcaggtcgag caagagcaac
     8701 gatgcgatca gcagcaccac cgtaggcatc atggaagcca gcatcacggt tagccatagc
     8761 ttccagtgcc acccccgcga cgcgctccgg gcgctctgcg cggcgctgct cacctcggcg
     8821 gctacctccc gcaactcttt ggccagctcc acccatgccg cccctgtctg gcgctgggct
     8881 ttcagccact ccgccgcctg cgcctcgctg gcctgctggg tctggctcat gacctgccgg
     8941 gcttcgtcgg ccagtgtcgc catgctctgg gccagcggtt cgatctgctc cgctaactcg
     9001 ttgatgcctc tggatttctt cactctgtcg attgcgttca tggtctattg cctcccggta
     9061 ttcctgtaag tcgatgatct gggcgttggc ggtgtcgatg ttcagggcca cgtctgcccg
     9121 gtcggtgcgg atgccccggc cttccatctc caccacgttc ggccccaggt gaacaccggg
     9181 caggcgctcg atgccctgcg cctcaagtgt tctgtggtca atgcgggcgt cgtggccagc
     9241 ccgctctaat gcccggttgg catggtcggc ccatgcctcg cgggtctgct caagccatgc
     9301 cttgggcttg agcgcttcgg tcttctgtgc cccgcccttc tccggggtct tgccgttgta
     9361 ccgcttgaac cactgagcgg cgggccgctc gatgccgtca ttgatccgct cggagatcat
     9421 caggtggcag tgcgggttct cgccgccacc ggcatggatg gccagcgtat acg
//
