LOCUS       CP082614               10384 bp    DNA     circular BCT 14-JAN-2022
DEFINITION  Salmonella enterica subsp. enterica serovar Reading strain CVM
            N18S0035 plasmid pN18S0035-2, complete sequence.
ACCESSION   CP082614
VERSION     CP082614.1
DBLINK      BioProject: PRJNA292661
            BioSample: SAMN12586937
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Reading
  ORGANISM  Salmonella enterica subsp. enterica serovar Reading
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Li,C., Tyson,G.H., Hsu,C.H., Harrison,L., Strain,E., Tran,T.T.,
            Tillman,G.E., Dessai,U., McDermott,P.F. and Zhao,S.
  TITLE     Long-Read Sequencing Reveals Evolution and Acquisition of
            Antimicrobial Resistance and Virulence Genes in Salmonella enterica
  JOURNAL   Front Microbiol 12, 777817 (2021)
   PUBMED   34867920
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10384)
  CONSRTM   NARMS: The National Antimicrobial Resistance Monitoring System
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2019) Center for Veterinary Medicine, US Food and
            Drug Administration, 8401 Muirkirk Rd, Laurel, MD, USA
REFERENCE   3  (bases 1 to 10384)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2020
            Assembly Method :: SMRTLink Microbial Assembly v. SMRTLink9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 616x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/31/2021 13:53:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,524
            CDSs (total)                      :: 4,404
            Genes (coding)                    :: 4,302
            CDSs (with protein)               :: 4,302
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 46 of 102
            Pseudo Genes (incomplete)         :: 56 of 102
            Pseudo Genes (internal stop)      :: 23 of 102
            Pseudo Genes (multiple problems)  :: 20 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Salmonella enterica subsp. enterica serovar
                     Reading"
                     /mol_type="genomic DNA"
                     /strain="CVM N18S0035"
                     /serovar="Reading"
                     /isolation_source="ground turkey"
                     /sub_species="enterica"
                     /db_xref="taxon:165302"
                     /plasmid="pN18S0035-2"
                     /geo_loc_name="USA:MO"
                     /collection_date="2018"
                     /collected_by="FDA"
     gene            complement(join(9571..10384,1..2192))
                     /locus_tag="FWK53_22575"
     CDS             complement(join(9571..10384,1..2192))
                     /locus_tag="FWK53_22575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="UAH46150.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            2356..2913
                     /locus_tag="FWK53_22580"
     CDS             2356..2913
                     /locus_tag="FWK53_22580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="UAH46151.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            3096..3956
                     /locus_tag="FWK53_22585"
     CDS             3096..3956
                     /locus_tag="FWK53_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="UAH46152.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="class A broad-spectrum beta-lactamase TEM-1"
     gene            complement(4627..4839)
                     /locus_tag="FWK53_22590"
     CDS             complement(4627..4839)
                     /locus_tag="FWK53_22590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UAH46153.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4843..5328)
                     /locus_tag="FWK53_22595"
     CDS             complement(4843..5328)
                     /locus_tag="FWK53_22595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UAH46154.1"
                     /translation="MSSLLALAKDLEKQSKAQQQNTCEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEQKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(5303..6043)
                     /locus_tag="FWK53_22600"
                     /pseudo=""
     CDS             complement(5303..6043)
                     /locus_tag="FWK53_22600"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6006..6326)
                     /locus_tag="FWK53_22605"
     CDS             complement(6006..6326)
                     /locus_tag="FWK53_22605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UAH46147.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNTGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGND
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7514..7975
                     /locus_tag="FWK53_22610"
     CDS             7514..7975
                     /locus_tag="FWK53_22610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAH46155.1"
                     /translation="MSKQDYIQLRTLTDQFYADNKGLQEALDGSNDGKVRGYGIVVIDL
                     NGLVFGIPLRSHLNHKFGFVSERSEGVKKGLDYTKALLIKKEEYVSRAYKIPTPEFTHI
                     NDNKEKIQEDFNKFVNRYIEANVKKDENILRNYRYSTLKNYHKELGLED"
                     /name="hypothetical protein"
     gene            complement(8004..8567)
                     /locus_tag="FWK53_22615"
     CDS             complement(8004..8567)
                     /locus_tag="FWK53_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAH46148.1"
                     /translation="MHALSILPSVARANLATKFSSHLKVIELFNTMQDSQVVVLSSLIE
                     GHHLTSSGNSVKADFEVTRLPAIIEMLEKKYFFPIRHLNVSVKSVTTGRMTGQTVYFIE
                     PEHIEQLLADPELVFANQERSLFFRSLEKEGKNLGKLIEKKGSVSQAVLSLLHHAYKDK
                     PLSDEMWKEIEDKFTHMLDELSAA"
                     /name="hypothetical protein"
     gene            complement(8825..9235)
                     /locus_tag="FWK53_22620"
     CDS             complement(8825..9235)
                     /locus_tag="FWK53_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAH46149.1"
                     /translation="MAFISLSNLAAKTGRIIQSQCVTATQTKKSTGAVELGFRLSKSAM
                     DETGITIGDRVDILYDKEENQWMIKKISEGGFGVSGQKNKHGGYSSCAVRLTLRPGFPR
                     LSEDEEDNKKLLSDDSFTQLSEGVIVFRLDDE"
                     /name="hypothetical protein"
     ARG             3097..3953
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(6435..6614)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.339"
                     /coverage="83.0"
                     /evalue="7.539999999999999e-48"
     Typing          6560..7073
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.217"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(4512..6016)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.426"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgccatgaa agccggaata ctgatcggac acaaagttgt accaggtgat ccctctgtta
       61 ttaccaaagt atttgcggtt cggtccggca ttgattgttc tgactggcgt aacaaagcgc
      121 attccatctg cagatgccac ttctcctcca ccccatatct gtgccagtgg cagcgttgcc
      181 tgaaaatcaa ccagtctggc attagcgctg gtgatagttt cagcccgcag atagttcgct
      241 tttgtccagt tcagccggtg tcgggtcagt gcaggaacat ttgatctgat cagtggttcc
      301 agaccgatat tgcaggcttc agccatcagc acggcgctga tgctgacggg cagatcatca
      361 actctggcac tggcttcact agcatggaaa aactcatcag caaatccggt atgggcgtta
      421 atttcgagca gcaactccgt taaatccacc ggagggagta gatcactgat cattttgctc
      481 agtcgtttca gactgtccgg ctcatcaaga ctggcgaggg gagaaattgt caaccggggc
      541 ttcgggccag aaacatcgag ttcgacagcc tcattttcgc aaagacgtgc agcaacctgt
      601 ctgtaacgac tatcaagctg atgacccaga gattttattg cttcctgcgg gtctgtcggg
      661 tgccccaaag aacgataaac cttaatccgg tttgcctgcc agtcagcacc ctgtagtaat
      721 cttgcacgag gatctcccca ccggttactg ccggtaacgt agacatccct ccgcctcaga
      781 ctatcctgca gtttactgag aaagcagagc gtgtatcccc tgcgggtgat atgtttttcc
      841 ttgttaatca ccagccgttt ccatgaccga ctgataattt ccgttggtgc gtcgtcaaaa
      901 aactgccgcc gtgagctgaa ctcccggctg aggtagtcac aggcattcag agtggtaacc
      961 ccggcaggtg cggatgaaaa tttaacggta ttcagcagat ggggcaggaa acgacgaacg
     1021 cgcccgtact gctccaccat ttcttcatga aaattatcgt ctgagggccg ggcaatttca
     1081 cggacaagcg tgatgatttc agccagcttt tgccttggga tgtagctgaa cacctcagca
     1141 cgaatcgatt cgtccggtgt ttcttctttc agcaggtacg aacatgcgct ggcgagcgcc
     1201 aatgcagatt tatccagatc cttcagcgag cggagccgtt ttttctgccc aatctttctg
     1261 gcgtcacgga tgataacggc cagcatggcg tccagaacgt ccaatgcatc atccagcgcc
     1321 agcgtttccc atgcaaggac aaaggcaacc agaaccgcca tccttttctg cggtgacatc
     1381 ctggcaatat tgaacaccga agtcatacca gcataacgtg cgagattttt caggcgcaca
     1441 gccgggagtg tactcaggtt ttcagcatgc aggccaaaat cgttcagagt tttccagcgt
     1501 tcaattgctt cattaaacgc cggaccactg atggtcacag ggcccttttt cagtgattcc
     1561 agtaaagaca ggcggctgca atcagttggc cccagcagca tctccagctg tgaacgctgt
     1621 tcggctgacg gtatcagtgc cagtttgttc cacaggcgca acgtcgcctt ttcccttacc
     1681 tctgaaatca accgggtcag cgtagtggct ccggggagaa taatacgatg ttgcataagc
     1741 caccctgtcg ccagatcgaa aagcaggcca ggacgttcgt tgcttatcca gctccgggta
     1801 tataaaagac gggtaaggcg aaatgtccag ggccaggcaa attcacgata ctgatagtgc
     1861 tgacgtatca gcgctgcatg ctcacggcgg gtattttccc tctgaccgta ttctgcaaga
     1921 acggtgatat cacgaatccc gagctgtctg gcggtaaaat gccggacgcc ggaaggaata
     1981 tgattcatat cggtgaggaa ggtgcccaga aaacggacac atccaatttg cagggcaatg
     2041 cccagacggt tgtgatcacc tctgcttttt ccgataaatt ccttgtctgc ttcatcaagg
     2101 tgaaaatatc gtgccagctg aagctcatcc ggttcaccgg tgaatctgcc atagctttca
     2161 gtctgctcag tggtcagaaa gtcaacgggc atatcggcct ccctgcctga cgggcattta
     2221 gtaacatttt tgcaaccgta cgaaatgtta taaattatcg gacatcgtaa aactgttaca
     2281 ttaatatgtc tattaaatcg taaatttgta ataatagaca tgagttgtcc gatattcgat
     2341 ttaaggtaca tttttatgcg actttttggt tacgctcggg tctcaaccag tcagcagtct
     2401 cttgatcttc aggtcagagc actcaaagac gcaggtgtga aagcaaaccg tatatttacc
     2461 gataaggcat ccggcagttc aacagaccgg gaagggctgg atttgctgag gatgaaggtg
     2521 gaggaaggtg atgtcattct ggttaagaag ctcgaccgtc ttggccgcga cactgccgat
     2581 atgatccaac tgataaagga atttgacgct cagggcgtgg cagtccggtt cattgatgac
     2641 gggatcagta ccgacggtga tatggggcaa atggtggtca ccatcctgtc ggctgtggca
     2701 caggctgaac gccggaggat cctagaacgc acgaatgagg gccgacagga agcaaagctg
     2761 aaaggaatca aatttggccg caggcgtacc gtggacagga acgtcgtgct gacgcttcat
     2821 cagaagggca ctggtgcaac ggaaattgct catcagctca gtattgcccg ctccacggtt
     2881 tataaaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     2941 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3001 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3061 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     3121 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     3181 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     3241 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     3301 cttttaaagt tctgctatgt ggtgcggtat tatcccgtgt tgacgccggg caagagcaac
     3361 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     3421 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     3481 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     3541 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     3601 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     3661 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     3721 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     3781 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3841 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3901 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3961 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     4021 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     4081 cgttccactg agcgtcagac cccgtatagg ttgccgatga gatgaattta acataagagt
     4141 tcttgcccgc accgcttcgg gctggttcgg cgcaaatacg gtgcctgtcg ggtgaaaccc
     4201 ctagcaaagc acccggtaaa ccgccccgtt tacgccagat aaatttcact tggctgcatg
     4261 gttatgcagc cagatgaatg acagcagttt tcgcgtataa atacggtgta aagcactaaa
     4321 aaggccactt tcccccgaat cgctcccaca gatccattgc cagtttgacg cattgcagta
     4381 tcagactgag cgcttttagt atcttcataa atccccctct cttgagccgt gatcttttca
     4441 tcacgaccgt atttagcggg aaatttatga gcccttcttc ttggggtttg tcactggatg
     4501 cgcgcagtca gcccagcgtc ggaccgttat agctccgctg acggaaacgg ggttctgcca
     4561 gctctttttc atgctgtcgc tgctcgtatg cccgttcggc ggccaccgcc atttcatgcc
     4621 gctgcactac tggctcaaac gctgtaactg cctgctcaag ttcgccacct gctgactcaa
     4681 gcgcgtgacg cgctcgttca gcgccgcgtt ctcccgttgc ataagaccag acatcttctg
     4741 ccattccgcg aaggcgctct cccactcgtc cagcctttgc gagtagtcct gatgtagctg
     4801 ctctaatgcg gtcagcaact gtttttccag ttccgtcatg ggctatttcc tctccagtac
     4861 catccagttc ccttcctccc cgtaagctcc cgctttcggg ttcactttga cgcaggacag
     4921 tttctgttcc ccacagagcg acagctgcac gccgtggttc ttctccgaca gcatggcctg
     4981 cgtccgctcc tgcgcccgga tagtctggta attcccggtg atctgctgcc cctgccacca
     5041 cagaatgctc ccgctcgtgg cgatcagcag tactgagacc agggtgatgg tcagccacgt
     5101 ccggccaacc atacgcagca cgctcagccg gttcgactgc atcgccgcgg tcataccctg
     5161 ctcgtgggcg ctgatggcgt cgctgattct cttcgcgctt gcgctcagtt ccgcttttac
     5221 agacttctcg tgctcgctga atgcggcttt cagcatctcg caggtattct gctgctgcgc
     5281 tttcgattgc ttttccaagt ccttcgccag cgctaaaaga ctgctcataa atggctccct
     5341 tcagtcggat gtttcgcccc ccgtccgggt cggcaatgct gatactgttc tttgtggtgc
     5401 ggaccacctc aaaacctgcc gcagtgagcg accctgtgac gtcctcgcgc gttttgagcg
     5461 ccccggatga ggctaaggcc agcaaacccc gtgtaatcgc ctgagcggct tccagcttcg
     5521 tttccggcag tgcggacggc gtgaccagta accgccggtt cttcggggcg tttggatcat
     5581 gcagccccag tctgccgttc accacggtct gccaggcatc gatgcgcggc cggtcggcgc
     5641 gatcgtaata cggctgcagg cgtttcccgg tcagcagctc tgtgttgggg atcaggaaat
     5701 tcagctccag ccgccccttg tcggtgtgct ccacccacag aatgctgtac tggtctttat
     5761 cgagcccggg catcagaacc cgctcaaagc tggccatgat ctgctcccgc tgcccgggcg
     5821 gcagctcagc ctccgcgaac gacagtacgc cggaggtgta ctttttcgca tacggcgagg
     5881 catcgataag ctcccggact tcctccggct ttccctgcag cacggtcgcc ccctcgcgct
     5941 gccggtcttt tcccagcagg taatccaccg gaccggcacc accaccgcgc ccccggggat
     6001 ggaatttaac gatcatcatt cccccctttt tccagcaccg catgccgcag ccgctccagt
     6061 cccgcgtcga ttgccatcag ggcggccacg atctgcaccc ggtcatgacc ggcaccaccg
     6121 gtattcactt tccgggcgat ctggttcagg ttattaccca taccggcaag ctggcgcagc
     6181 agcgccggcg atatcgacgg cagtctgccg gcacgcgccg gcttctcatc gaggcaggtc
     6241 tgacgcatcc acgccgccag ctgcttgccg tcgcaccgct cgagcagccg ccggtgttcg
     6301 tcttccgtca cccacatcgt gagcatcttg ttgcgtttgt ctgccagagt agcctcctga
     6361 taacaccggg acgggcggga aatgtttccc ggtcggcacg gtgttggaca agccgcaggc
     6421 gcgtcagtgg cttttagcgg gtttcggggc gcagccctga accagtcacg tagcgctagc
     6481 ggagtgtata ctggcttaat atgttacggc ggagtaacac tttataaaag tgcttcatgt
     6541 ggcaggggaa aatgcagctg cagggcgtac gggagaacat ggaggttgcg gtgatattac
     6601 gcctcctcgc tcactcggtc gcttccgctc ctgccgttcg gctgcggcgg gcgttacctg
     6661 cacacggata agagcaaagg catacggcat gacaaacagg gcaagaaaag gatgcggcgc
     6721 tgccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
     6781 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggtggctccc
     6841 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     6901 gtttatctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     6961 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tttccggtaa ctatcgactt
     7021 gagtccaacc cggaaagaca cgactaatcg ccattggcaa cggccactgg taaacatgat
     7081 gtgcgagaga tgtattgaga gttcttgaag tggtggcctt actgaggcta cactggaagg
     7141 acggatttgg tgacacgtct cgcacaagac tgttaccacg gttagataat ctgccaggat
     7201 taaataacct tcgaaaaacc acctgccagg gtggtttttt cgtttcagga gaagattacg
     7261 ctggcaaagt aagtccagtc aagaacagct acgaaattga cttcacatat aatttttgat
     7321 ataagtaaat ctagaagtgg cggtaggaat gagccccacg ttaaacaaac aagcttccaa
     7381 agaaagatgt gcggtagagt tcgccacacg taaaaaatcc aagactcaac gaaagaaaag
     7441 caaagccctc tcttacagag ggctttgctt tttttgtaca ttggttacag gaaaatgcca
     7501 atggacatca aaaatgagca aacaggatta catacagtta agaaccctta cagaccagtt
     7561 ttatgctgat aacaaaggcc tacaagaggc tctcgacggc agtaatgatg gaaaagtaag
     7621 aggctacgga atcgtagtta ttgatctcaa cggattggtg ttcgggatac ccttacgaag
     7681 ccatttaaac cataaatttg gattcgtttc ggagcgatca gaaggtgtta agaaaggact
     7741 ggattacacc aaagcgttgc taattaagaa agaagagtat gtatcgcgag cctataagat
     7801 accaactcct gaatttaccc atatcaatga taacaaagaa aaaatacaag aagatttcaa
     7861 caaattcgta aacagataca tagaagcgaa cgtcaagaaa gatgaaaaca tactcagaaa
     7921 ctatcgttac tctacgctga aaaactatca caaagaatta ggtttagaag actaaaaagg
     7981 cggtctaacg accgcccttg cctttacgcc gcgctcaatt catccagcat atgggtgaac
     8041 ttatcttcta tctccttcca catctcgtca gaaagaggtt tatctttgta ggcgtgatgt
     8101 agaaggctta agacggcttg agaaacacta ccttttttct cgataagctt gccgagattt
     8161 ttcccttctt tttcgagaga acgaaagaag aggctccttt cctgattagc aaacacaagc
     8221 tcaggatcag cgagaagttg ctcgatgtgt tcaggctcta tgaaatagac agtttgtcca
     8281 gtcatccttc ctgtagttac agatttaaca gatacattta gatgacgaat agggaaaaaa
     8341 tattttttct ctaacatctc gataatcgcc ggtaagcgag tgacctcaaa gtcggctttg
     8401 acactgttac cagaactggt gaggtgatgg ccttcgatca gtgatgacaa gacaacaact
     8461 tgggaatcct gcatcgtgtt gaagagctcg atcaccttca aatgagacga gaatttagtt
     8521 gccaggttag cgcgggcaac tgaaggtagt attgataaag cgtgcatatg tatagcctca
     8581 tatgttttta aaaacataaa cttaagagac caaagccaat agcatcgcac ctatcagaat
     8641 agaaaatgtc gtgttcacat ccctcaaaag aaacgatttt ttctttatta gaatgcaagt
     8701 ttatgaacat aatgccgccc ataacactcc acccttccaa aggtagtgat gtgttgacaa
     8761 gaaggcggag ttagtcgatt gtttggcgac gctcactaac tctgcctttc ctttttggtc
     8821 cttcctattc atcatccagc ctgaagacga ttacaccttc acttagctga gtgaatgaat
     8881 catcggaaag tagcttcttg ttgtcctctt catcctcaga aaggcgagga aaacctggtc
     8941 gcaaggtcaa tctaacagca cacgatgagt aaccgccgtg cttgtttttt tgcccagaca
     9001 caccaaagcc accttcacta attttcttaa tcatccactg gttttcttct ttgtcgtaaa
     9061 gaatgtcgac acgatcccca atggtaatac cagtctcgtc catagcgctc ttgctcaagc
     9121 ggaatcccag ctcgacagcg cccgtgcttt ttttcgtttg cgtggccgta acacactgcg
     9181 actggattat ccttccagtc ttcgctgcca agttcgatag agagataaaa gccataatac
     9241 aatccgtttt agacatcgat cagttcttat acggaagtgt acccagggag cgaggtggct
     9301 ttcaagatct tttgtattga tttttctaga gccgcaaaaa acggcgataa gaaaccttaa
     9361 atataaaaac aattgctcgt attgaacctc gtcaagatac acatccagat cgatctttat
     9421 gtaagtaaag ggtatttttc tatggtgatt taaagtcgag caacgtcagc gcatccaaaa
     9481 aatcaccaag taggtgcaag aaagccgctt atagaaactg atttttatgc ctgtataggg
     9541 gtctgacgct cagtggaacg aaaactcacg ttaagcaacg ttttctgcct ctgacgcctc
     9601 ttttaatggt ctcagatgtc ctttggtcac cagttctgcc agcgtgaagg aataatggcc
     9661 gagcatattg atatgtccgt ggcaaagcgg ggagaggcgt gcgatatctt catcattcag
     9721 tgtttcaccc tgcgcccgga gatgatccag ggctgcctgc atataaatag tgttccataa
     9781 cacgacggcg ttagtgacca gccccagtgc gcccagttga tcttcctgac cgtcggtata
     9841 tcgttttctt atctcacctt tttgaccgtg acagatggct ctggcaacgg catggcgact
     9901 ttctccccga ttaagctggg tcagaatgcg ccggcggtaa tcttcatcat caatataatt
     9961 aagcagatac agcgttttgt tgatgcgccc cacttcaatg attgcctgag tcagtccgga
    10021 aggacgttca cttttcagca atgaacggac cagcactgaa gcctgtactt tgcccagctt
    10081 cagggagcca gcggtccgga tcatttcgtc ccactgaagg actatttttc ggggatctga
    10141 ttgccctctg gcaatatcat tcagcacgcc atagtcggca tcatggtcca ttcgccagaa
    10201 aaccgaagca ccggcatcag ccaggcgtgg agaaaactgg tatcccagca gccagaaaag
    10261 gccaaagaca agttcgctgg cacctgctgt atcggtcata atttcggttg gattcagccc
    10321 ggtctcctgt tccagaagac cttccagcac aaagatagag tccctcagcg tccccggtat
    10381 aacg
//
