LOCUS       CP082612               10911 bp    DNA     circular BCT 14-JAN-2022
DEFINITION  Salmonella enterica subsp. enterica serovar Reading strain CVM
            N18S0035 plasmid pN18S0035-1, complete sequence.
ACCESSION   CP082612
VERSION     CP082612.1
DBLINK      BioProject: PRJNA292661
            BioSample: SAMN12586937
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Reading
  ORGANISM  Salmonella enterica subsp. enterica serovar Reading
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 10911)
  AUTHORS   Li,C., Tyson,G.H., Hsu,C.H., Harrison,L., Strain,E., Tran,T.T.,
            Tillman,G.E., Dessai,U., McDermott,P.F. and Zhao,S.
  TITLE     Long-Read Sequencing Reveals Evolution and Acquisition of
            Antimicrobial Resistance and Virulence Genes in Salmonella enterica
  JOURNAL   Front Microbiol 12, 777817 (2021)
   PUBMED   34867920
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10911)
  CONSRTM   NARMS: The National Antimicrobial Resistance Monitoring System
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2019) Center for Veterinary Medicine, US Food and
            Drug Administration, 8401 Muirkirk Rd, Laurel, MD, USA
REFERENCE   3  (bases 1 to 10911)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2020
            Assembly Method :: SMRTLink Microbial Assembly v. SMRTLink9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 616x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/31/2021 13:53:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,524
            CDSs (total)                      :: 4,404
            Genes (coding)                    :: 4,302
            CDSs (with protein)               :: 4,302
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 46 of 102
            Pseudo Genes (incomplete)         :: 56 of 102
            Pseudo Genes (internal stop)      :: 23 of 102
            Pseudo Genes (multiple problems)  :: 20 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10911
                     /organism="Salmonella enterica subsp. enterica serovar
                     Reading"
                     /mol_type="genomic DNA"
                     /strain="CVM N18S0035"
                     /serovar="Reading"
                     /isolation_source="ground turkey"
                     /sub_species="enterica"
                     /db_xref="taxon:165302"
                     /plasmid="pN18S0035-1"
                     /geo_loc_name="USA:MO"
                     /collection_date="2018"
                     /collected_by="FDA"
     gene            complement(join(9233..10911,1..451))
                     /locus_tag="FWK53_22495"
     CDS             complement(join(9233..10911,1..451))
                     /locus_tag="FWK53_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UAH46137.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHG
                     LVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEY
                     DADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQI
                     EQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQK
                     LASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPA
                     PRQRGMDRGGPDFSM"
                     /name="MobA/MobL family protein"
     gene            650..934
                     /locus_tag="FWK53_22500"
     CDS             650..934
                     /locus_tag="FWK53_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="UAH46138.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            1532..1720
                     /locus_tag="FWK53_22505"
                     /pseudo=""
     CDS             1532..1720
                     /locus_tag="FWK53_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(1687..2886)
                     /gene="tet(A)"
                     /locus_tag="FWK53_22510"
     ARG             complement(1691..2886)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2992..3642
                     /gene="tetR(A)"
                     /locus_tag="FWK53_22515"
     CDS             2992..3642
                     /gene="tetR(A)"
                     /locus_tag="FWK53_22515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="UAH46143.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(3674..3916)
                     /locus_tag="FWK53_22520"
     CDS             complement(3674..3916)
                     /locus_tag="FWK53_22520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="UAH46140.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="relaxase"
     gene            complement(4222..5058)
                     /locus_tag="FWK53_22525"
     CDS             complement(4222..5058)
                     /locus_tag="FWK53_22525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="UAH46141.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     ARG             complement(5059..5477)
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             complement(5062..5861)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(5922..6737)
                     /gene="sul2"
                     /locus_tag="FWK53_22535"
     ARG             complement(5926..6737)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(7045..7896)
                     /locus_tag="FWK53_22540"
     CDS             complement(7045..7896)
                     /locus_tag="FWK53_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="UAH46133.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="replication protein"
     gene            complement(7883..8722)
                     /locus_tag="FWK53_22545"
     CDS             complement(7883..8722)
                     /locus_tag="FWK53_22545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="UAH46134.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            complement(8752..8958)
                     /locus_tag="FWK53_22550"
     CDS             complement(8752..8958)
                     /locus_tag="FWK53_22550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAH46135.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="hypothetical protein"
     gene            complement(8960..9172)
                     /locus_tag="FWK53_22555"
     CDS             complement(8960..9172)
                     /locus_tag="FWK53_22555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAH46136.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     ARG             complement(4226..5058)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(541..696)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          complement(7884..8705)
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(9237..10909)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tctccggggt cttgccgttg taccgcttga accactgagc ggcgggccgc tcgatgccgt
       61 cattgatccg ctcggagatc atcaggtggc agtgcgggtt ctcgccgcca ccggcatgga
      121 tggccagcgt atacggcagg cgctcggcac cggtcaggtg ctgggcgaac tcggacgcca
      181 gcgccttctg ctggtcgagg gtcagctcga ccggcagggc aaattcgacc tccttgaaca
      241 gccgcccatt ggcgcgttca tacaggtcgg cagcatccca gtagtcggcg ggccgctcga
      301 cgaactccgg catgtgcccg gattcggcgt gcaagacttc atccatgtcg cgggcatact
      361 tgccttcgcg ctggatgtag tcggccttgg ccctggccga ttggccgccc gacctgctgc
      421 cggttttcgc cgtaaggtga taaatcgcca tgctgcctcg ctgttgcttt tgcttttcgg
      481 ctccatgcaa tggccctcgg agagcgcacc gcccgaaggg tggccgttag gccagtttct
      541 cgaagagaaa ccggtaagtg cgccctcccc tacaaagtag ggtcgggatt gccgccgctg
      601 tgcctccatg atagcctacg agacagcaca ttaacaatgg ggtgtcaaga tggttaaggg
      661 gagcaacaag gcggcggatc ggctggccaa gctcgaagaa caacgagcgc gaatcaatgc
      721 cgaaattcag cgggtgcggg caagggaaca gcagcaagag cgcaagaacg aaacaaggcg
      781 caaggtgctg gtgggggcca tgattttggc caaggtgaac agcagcgagt ggccggagga
      841 tcggctcatg gcggcaatgg atgcgtacct tgaacgcgac cacgaccgcg ccttgttcgg
      901 tctgccgcca cgccagaagg atgagccggg ctgaatgatc gaccgagaca ggccctgcgg
      961 ggctgcacac gcgcccccac ccttcgggta gggggaaagg ccgctaaagc ggctaaaagc
     1021 gctccagcgt atttctgcgg ggtttggtgt ggggtttagc gggctttgcc cgcctttccc
     1081 cctgccgcgc agcggtgggg cggtgtgtag cctagcgcag cgaatagacc agctatccgg
     1141 cctctggccg ggcatattgg gcaagggcag cagcgcccca caagggcgct gataaccgcg
     1201 cctagtggat tattcttaga taatcatgga tggatttttc caacaccccg ccagcccccg
     1261 cccctgctgg gtttgcaggt ttgggggcgt gacagttatt gcaggggttc gtgacagtta
     1321 ttgcaggggg gcgtgacagt tattgcaggg gttcgtgaca gttattgcag gggggcgtga
     1381 cagttattgc aggggttcgt gacagttagt acgggagtga cgggcactgg ctggcaatgt
     1441 ctagcaacgg caggcatttc ggctgagggt aaaagaactt tccgctaagc gatagactgt
     1501 atgtaaacac agtattgcaa ggacgcggaa catgcctcat gtggcggcca ggacggccag
     1561 ccgggatcgg gatactggtc gttaccagag ccaccgaccc gagcaaaccc ttctctatca
     1621 gatcgttgac gagtattacc cggcattcgc tgcgcttatg gcagagcagg gaaaggaatt
     1681 gccgggctat gtgcaacggg aatttgaaga atttctccaa agcccgcgac gcagcgccgg
     1741 caggcagagc aagtagaggg cagcgcctgc aatccatgcc cacccgttcc acgttgttat
     1801 agaagccgca tagatcgccg tgaagaggag gggtccgacg atcgaggtca ggctggtgag
     1861 cgccgccagt gagccttgca gctgcccctg acgttcctca tccacctgcc tggacaacat
     1921 tgcttgcagc gccggcattc cgatgccacc cgaagcaagc aggaccatga tcgggaacgc
     1981 catccatccc cgtgtcgcga aggcaagcag gatgtagcct gtgccgtcgg caatcattcc
     2041 gagcatgagt gcccgccttt cgccgagccg ggcggctaca gggccggtga tcattgcctg
     2101 ggcgagtgaa tgcagaatgc caaatgcggc aagcgaaatg ccgatcgtgg tcgcgtccca
     2161 gtgaaagcga tcctcgccga aaatgaccca aagcgcggcc ggcacctgtc cgacaagttg
     2221 catgatgaag aagaccgcca tcagggcggc gacgacggtc atgccccggg cccaccggaa
     2281 cgaagcgagc gggttgagag cctcccggcg taacggccgg cgttcgcctt tgtgcgactc
     2341 cggcaaaagg aaacagcccg tcaggaaatt gaggccgttc aaggctgccg cggcgaagaa
     2401 cggagcgtgg ggggagaaac cgcccatcag cccaccgagc acaggtcccg cgaccatccc
     2461 gaacccgaaa caggcgctca tgaagccgaa gtgccgcgcg cgctcatcgc catcagtgat
     2521 atcggcaata taagcgccgg ctaccgcccc agtcgccccg gtgatgccgg ccacgatccg
     2581 cccgatatag agaacccaaa ggaaaggcgc cgtcgccatg atggcgtagt cgacagcagc
     2641 gccggccagc gagacgagca agaccggccg ccgcccgaaa cgatccgaca gcgcgcccag
     2701 cacaggtgcg caggcaaatt gcatcaacgc atacagcgcc agcagaatgc catagtgggc
     2761 ggtgacgtcg ttcgagtgaa ccagatcgcg caggaggccc ggcagcaccg gcataatcag
     2821 gccgatgccg acagcgtcga gcgcgacagt gctcagaatt acgatcaggg gtctgttggg
     2881 tttcacgtct ggcctccgga ccagcctccg ctggtccgat tgaacgcgcg gattctttat
     2941 cactgataag ttggtggaca tattatgttt atcagtgata aagtgtcaag catgacaaag
     3001 ttgcagccga atacagtgat ccgtgccgcc ctggacctgt tgaacgaggt cggcgtagac
     3061 ggtctgacga cacgcaaact ggcggaacgg ttgggggttc agcagccggc gctttactgg
     3121 cacttcagga acaagcgggc gctgctcgac gcactggccg aagccatgct ggcggagaat
     3181 catacgcatt cggtgccgag agccgacgac gactggcgct catttctgat cgggaatgcc
     3241 cgcagcttca ggcaggcgct gctcgcctac cgcgatggcg cgcgcatcca tgccggcacg
     3301 cgaccgggcg caccgcagat ggaaacggcc gacgcgcagc ttcgcttcct ctgcgaggcg
     3361 ggtttttcgg ccggggacgc cgtcaatgcg ctgatgacaa tcagctactt cactgttggg
     3421 gccgtgcttg aggagcaggc cggcgacagc gatgccggcg agcgcggcgg caccgttgaa
     3481 caggctccgc tctcgccgct gttgcgggcc gcgatagacg ccttcgacga agccggtccg
     3541 gacgcagcgt tcgagcaggg actcgcggtg attgtcgatg gattggcgaa aaggaggctc
     3601 gttgtcagga acgttgaagg accgagaaag ggtgacgatt gatcaggacc gctgccggag
     3661 cgcaacccac tcactacagc agagccatgt agacaacatc ccctccccct ttccaccgcg
     3721 tcagacgccc gtagcagccc gctacgggct ttttcatgcc ctgccctagc gtccaagcct
     3781 cacggccgcg ctcggcctct ctggcggcct tctggcgctc ctgctgcggc gtccgctcgt
     3841 gggccgtggc gcgggtccgc gcgccggcct cgtgcgcctg gcgctcgcgg gcgaggtcca
     3901 gggcggccgt cttcacgttc tgccttgcgc agatgagata gatctagcgt ggactcaagg
     3961 ctctcgcgaa tggctcgcgt tggaaacttt cattgacact tgaggggcac cgcagggaaa
     4021 ttctcgtcct tgcgagaacc ggctatgtcg tgctgcgcat cgagcctgcg cccttggctt
     4081 gtctcgcccc tctccgcgtc gctacggggc ttccaggggt cgtttgcggg aaggggcgga
     4141 atcctacgct aaggctttgg ccagcgatat tctccggtga gattgatgtg ttcccagggg
     4201 ataggagaag tcgcttgata tctagtatga cgtctgtcgc acctgcttga tcgcggccgc
     4261 gatagctaga tcgcgttgct cctcttctcc atccgcgttc caagctgcgg aaaggcaccc
     4321 ataagcgtac gcctggtcga gcaggcgacg cggatcgacg tccagcgcac gagagaatgc
     4381 gtccgccatc tgtgcaatgc gtctaggatc gagacaaagg tcgtctctgt cagccggatc
     4441 gtagaacata ttggcggcgc caaagcccac ttcaccgacc agaccgacgg gatctatcac
     4501 cagccagccg cgactggaga acatgatgtt ttcatgatgc agatcgccat gtagcccacg
     4561 cagttccgag gcattgctca tcatttgatc ggctataatc gccgcgtgga cgtagtcagt
     4621 ttgacaacct gcgttttgat catcgcgcgc ccgctgaaac aaagctgcaa agcgatcccg
     4681 gatcgggaga agggcagaag gcaggggttc ctcagatgcg gcatacagct tcgccattag
     4741 ttccgctgca atttcggtcg cctggtagtc gccgtgctcg gcaacgatgt gagagagcat
     4801 tcgctccccg gcatattcga gcaacatcag attgttctca cgaccgagca accggactgc
     4861 tcccctccca ttgcgccata ccagatagtc ggccccgcgc agttcatcag caatgtcttc
     4921 tataggtttc aatcccttga cgattgcagg agtcccgtct ggcaatgaaa ctttccaaac
     4981 gaggctggaa aaggtgtccg caatgagaac aggttgcgaa acgtgccaat gagcaggaaa
     5041 aacaggcggc atgaacatca accccaagtc agagggtcca atcgcagata gaaggcaagg
     5101 cgttcgcggt cgggggcttc gatccccaat acattgaata ggacagcgaa ggcgcgctct
     5161 gcttcatctg gcgctgccca gttctcttcg gcgttagcaa tcatgagtgc caaatcggca
     5221 tagcgatctg ctgttccgag ccgcccaagg tcgatcagac ccgtgcattg aagagtttta
     5281 gggtccacca tgaagttcgg catgcaggga tcaccatggc aaacaaccat atcggtgcgc
     5341 tcttggtcga gccgcaccgg tagctctcgt tcgacacgag ccaaaagatc gagctgcggc
     5401 gtactcttgt cctcgtccgg taagaagtcg ggattgacgg cattgcggga caccacatca
     5461 acggcgcgtc cgaacattcg cgacagcctg cgctcaaacg gacattgatc aaccgatagg
     5521 ctgtgaacag cgccaagttg ctgccccatt gacggccacg ctttgagcaa atccgctcca
     5581 gacagatcag ccgccggtac tcccggaatt gccgttatca ccaagcatgc accctcctgt
     5641 tcctcctgcc agttgatgac ctcggggcaa gccacacctc gacctttgag ccaaatgagg
     5701 cggtcacgct ctccagcgag ctcaccgcgg cgggaagcag gtgcgatttt cgcgaaggca
     5761 tgcccgtcac cacgtcgaaa aacaaaatca ccagattctc cgcctctgac aggcaaccag
     5821 tcagaatgcg attcaccaaa aaaaatatta gttcgattca atggaggttc cttcagtttt
     5881 ctgatgaagc gcgaatatag agaaatatcc cgaatgtgca gttaacgaat tcttgcggtt
     5941 tctttcagcg ccgccaatac cgccagcccg tcgcgcaagg ggcgcggctc gtgtgtgcgg
     6001 atgaagtcag ctccacctgc ggcggcggca agctctgcag cgagtgtcgc ggccccgaca
     6061 tcccccggac cacggcctgt gagcgcgcgc agaaaggatt tgcgcgaaac agacagaagc
     6121 accggcaaat cgaagcgcag ccgcaattca tcgaaccgcg ccagcaccga gagcgaggtt
     6181 tcgggagcag cccccagaaa aaaccccatg ccgggatcaa ggacaaggcg gttgcgtttg
     6241 ataccggcac ccgtcagcgc cgcgatgcgc gcgtcaaaga acgccgcaat gtgatccatg
     6301 atgtcgccag cgggtgcctc gcgccgatct gcctgcccgt cttgcaccga atgcataacg
     6361 acgagtttgg cagatgattt cgccaattgc ggatagaacg cagcgtctgg aaaaccgcga
     6421 atatcattga gataggccac accacgcgac aaggcatagg cttgcgtcgc gggttgataa
     6481 ctgtcgagcg agacgggaat gccatctgcc ttgagcgcgt ccagcaccgg cgcgatacgc
     6541 gcgatttctg tgtcggacga aacaggcgcg gcgtcgggat tgctggatgc cggaccgagg
     6601 tcgatcacat ctgccccctc ggccatcagc ttacgcgcct gcgcaatggc tgcgtctggc
     6661 gccagatacc ggcctccatc ggagaaactg tccgaggtta tgttgacgat gccgaaaatg
     6721 atgagcgatt tattcatggg ggcttctata ataataataa tcgagcatga gtctcatacg
     6781 gatgctcggg tcgaaaggga atccccaggc gagtaacctg tttgcggtga tccattagct
     6841 gcaggagcag aagagcatac atctggaagc aaagccagga aagcggccta tggagctgtg
     6901 cggcagcgct cagtaggcaa tttttcaaaa tattgttaag ccttttctga gcatggtatt
     6961 tttcatggta ttaccaatta gcaggaaaat aagccattga atataaaaga taaaaatgtc
     7021 ttgtttacaa tagagtgggg ggggtcagcc tgccgccttg ggccgggtga tgtcgtactt
     7081 gcccgccgcg aactcggtta ccgtccagcc cagcgcgacc agctccggca acgcctcgcg
     7141 cacccgctgg cggcgcttgc gcatggtcga accactggcc tctgacggcc agacatagcc
     7201 gcacaaggta tctatggaag ccttgccggt tttgccgggg tcgatccagc cacacagccg
     7261 ctggtgcagc aggcgggcgg tttcgctgtc cagcgcccgc acctcgtcca tgctgatgcg
     7321 cacatgctgg ccgccaccca tgacggcctg cgcgatcaag gggttcaggg ccacgtacag
     7381 gcgcccgtcc gcctcgtcgc tggcgtactc cgacagcagc cgaaacccct gccgcttgcg
     7441 gccattctgg gcgatgatgg ataccttcca aaggcgctcg atgcagtcct gtatgtgctt
     7501 gagcgcccca ccactatcga cctctgcccc gatttccttt gccagcgccc gatagctacc
     7561 tttgaccacc atggcatcag cggtgacggc ctcccacttg ggttccagga acagccggag
     7621 ctgccgtccg ccttcggtct tgggttccgg gccaagcact aggccattag gcccagccat
     7681 ggccaccagc ccttgcagga tgcgcagatc atcagcgccc agcggctccg ggccgctgaa
     7741 ctcgatccgc ttgccgtcgc cgtagtcata cgtcacgtcc agcttgctgc gcttgcgctc
     7801 gccccgcttg agggcacgga acaggccggg ggccagacag tgcgccgggt cgtgccggac
     7861 gtggctgagg ctgtgcttgt tcttaggctt caccacgggg cacccccttg ctcttgcgct
     7921 gcctctccag cacggcgggc ttgagcaccc cgccgtcatg ccgcctgaac caccgatcag
     7981 cgaacggtgc gccatagttg gccttgctca caccgaagcg gacgaagaac cggcgctggt
     8041 cgtcgtccac accccattcc tcggcctcgg cgctggtcat gctcgacagg taggactgcc
     8101 agcggatgtt atcgaccagt accgagctgc cccggctggc ctgctgctgg tcgcctgcgc
     8161 ccatcatggc cgcgcccttg ctggcatggt gcaggaacac gatagagcac ccggtatcgg
     8221 cggcgatggc ctccatgcga ccgatgacct gggccatggg gccgctggcg ttttcttcct
     8281 cgatgtggaa ccggcgcagc gtgtccagca ccatcaggcg gcggccctcg gcggcgcgct
     8341 tgaggccgtc gaaccactcc ggggccatga tgttgggcag gctgccgatc agcggctgga
     8401 tcagcaggcc gtcagccacg gcttgccgtt cctcggcgct gaggtgcgcc ccaagggcgt
     8461 gcaggcggtg atgaatggcg gtgggcgggt cttcggcggg caggtagatc accgggccgg
     8521 tgggcagttc gcccacctcc agcagatccg gcccgcctgc aatctgtgcg gccagttgca
     8581 gggccagcat ggatttaccg gcaccaccgg gcgacaccag cgccccgacc gtaccggcca
     8641 ccatgttggg caaaacgtag tccagcggtg gcggcgctgc tgcgaacgcc tccagaatat
     8701 tgataggctt atgggtagcc attgattgcc tcctttgcag gcagttggtg gttaggcgct
     8761 ggcggggtca ctacccccgc cctgcgccgc tctgagttct tccaggcact cgcgcagcgc
     8821 ctcgtattcg tcgtcggtca gccagaactt gcgctgacgc atccctttgg ccttcatgcg
     8881 ctcggcatat cgcgcttggc gtacagcgtc agggctggcc agcaggtcgc cggtctgctt
     8941 gtccttttgg tctttcatat cagtcaccga gaaacttgcc ggggccgaaa ggcttgtctt
     9001 cgcggaacaa ggacaaggtg cagccgtcaa ggttaaggct ggccatatca gcgactgaaa
     9061 agcggccagc ctcggccttg tttgacgtat aaccaaagcc accgggcaac caatagccct
     9121 tgtcactttt gatcaggtag accgaccctg aagcgctttt ttcgtattcc ataaaacccc
     9181 cttctgtgcg tgagtactca tagtataaca ggcgtgagta ccaacgcaag cactacatgc
     9241 tgaaatctgg cccgcccctg tccatgcctc gctggcgggg tgccggtgcc cgtgccagct
     9301 cggcccgcgc aagctggacg ctgggcagac ccatgacctt gctgacggtg cgctcgatgt
     9361 aatccgcttc gtggccgggc ttgcgctctg ccagcgctgg gctggcctcg gccatggcct
     9421 tgccgatttc ctcggcactg cggccccggc tggccagctt ctgcgcggcg ataaagtcgc
     9481 acttgctgag gtcatcaccg aagcgcttga ccagcccggc catctcgctg cggtactcgt
     9541 ccagcgccgt gcgccggtgg cggctaagct gccgctcggg cagttcgagg ctggccagcc
     9601 tgcgggcctt ctcctgctgc cgctgggcct gctcgatctg ctggccagcc tgctgcacca
     9661 gcgccgggcc agcggtggcg gtcttgccct tggattcacg cagcagcacc cacggctgat
     9721 aaccggcgcg ggtggtgtgc ttgtccttgc ggttggtgaa gcccgccaag cggccatagt
     9781 ggcggctgtc ggcgctggcc gggtcggcgt cgtactcgct ggccagcgtc cgggcaatct
     9841 gcccccgaag ttcaccgcct gcggcgtcgg ccaccttgac ccatgcctga tagttcttcg
     9901 ggctggtttc cactaccagg gcaggctccc ggccctcggc tttcatgtca tccaggtcaa
     9961 actcgctgag gtcgtccacc agcaccagac catgccgctc ctgctcggcg ggcctgatat
    10021 acacgtcatt gccctgggca ttcatccgct tgagccatgg cgtgttctgg agcacttcgg
    10081 cggctgacca ttcccggttc atcatctggc cggtggtggc gtccctgacg ccgatatcga
    10141 agcgctcaca gcccatggcc ttgagctgtc ggcctatggc ctgcaaagtc ctgtcgttct
    10201 tcatcgggcc accaagcgca gccagatcga gccgtcctcg gttgtcagtg gcgtcaggtc
    10261 gagcaagagc aacgatgcga tcagcagcac caccgtaggc atcatggaag ccagcatcac
    10321 ggttagccat agcttccagt gccacccccg cgacgcgctc cgggcgctct gcgcggcgct
    10381 gctcacctcg gcggctacct cccgcaactc tttggccagc tccacccatg ccgcccctgt
    10441 ctggcgctgg gctttcagcc actccgccgc ctgcgcctcg ctggcctgct gggtctggct
    10501 catgacctgc cgggcttcgt cggccagtgt cgccatgctc tgggccagcg gttcgatctg
    10561 ctccgctaac tcgttgatgc ctctggattt cttcactctg tcgattgcgt tcatggtcta
    10621 ttgcctcccg gtattcctgt aagtcgatga tctgggcgtt ggcggtgtcg atgttcaggg
    10681 ccacgtctgc ccggtcggtg cggatgcccc ggccttccat ctccaccacg ttcggcccca
    10741 ggtgaacacc gggcaggcgc tcgatgccct gcgcctcaag tgttctgtgg tcaatgcggg
    10801 cgtcgtggcc agcccgctct aatgcccggt tggcatggtc ggcccatgcc tcgcgggtct
    10861 gctcaagcca tgccttgggc ttgagcgctt cggtcttctg tgccccgccc t
//
