LOCUS       CP082586                4585 bp    DNA     circular BCT 13-JAN-2022
DEFINITION  Salmonella enterica subsp. enterica serovar Kiambu strain CVM
            N18S0824 plasmid pN18S0824-2, complete sequence.
ACCESSION   CP082586
VERSION     CP082586.1
DBLINK      BioProject: PRJNA292661
            BioSample: SAMN12360055
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Kiambu
  ORGANISM  Salmonella enterica subsp. enterica serovar Kiambu
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Li,C., Tyson,G.H., Hsu,C.H., Harrison,L., Strain,E., Tran,T.T.,
            Tillman,G.E., Dessai,U., McDermott,P.F. and Zhao,S.
  TITLE     Long-Read Sequencing Reveals Evolution and Acquisition of
            Antimicrobial Resistance and Virulence Genes in Salmonella enterica
  JOURNAL   Front Microbiol 12, 777817 (2021)
   PUBMED   34867920
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  CONSRTM   NARMS: The National Antimicrobial Resistance Monitoring System
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2019) Center for Veterinary Medicine, US Food and
            Drug Administration, 8401 Muirkirk Rd, Laurel, MD, USA
REFERENCE   3  (bases 1 to 4585)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2020
            Assembly Method :: SMRTLink Microbial Assembly v. SMRTLink9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 420x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/01/2021 10:27:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,507
            CDSs (total)                      :: 4,388
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 38 of 75
            Pseudo Genes (incomplete)         :: 44 of 75
            Pseudo Genes (internal stop)      :: 15 of 75
            Pseudo Genes (multiple problems)  :: 20 of 75
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Salmonella enterica subsp. enterica serovar
                     Kiambu"
                     /mol_type="genomic DNA"
                     /strain="CVM N18S0824"
                     /serovar="Kiambu"
                     /isolation_source="ground turkey"
                     /sub_species="enterica"
                     /db_xref="taxon:682797"
                     /plasmid="pN18S0824-2"
                     /geo_loc_name="USA:GA"
                     /collection_date="2018"
                     /collected_by="FDA"
     gene            complement(join(3732..4585,1..682))
                     /locus_tag="FQC27_000355"
     CDS             complement(join(3732..4585,1..682))
                     /locus_tag="FQC27_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UAH92731.1"
                     /translation="MASYHCTVKTGAKGAALKHADYISRSGEYKSYKSREDLEFSSSGN
                     MPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGNRH
                     AYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKKASI
                     PQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGIKRTPEVHLGPVHAA
                     NLNEGQIAAIQEHRAARQQERAARNPAPERQQELEQKQKIRTVERSPELLAQYRKVMKT
                     VIQGEERLARLGDANPDALKEHKLLQNAKAKKESLSEWSRRIYEGARYLDKLGRNVVSA
                     QRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHERNEFRNKLQQAES
                     EWDKENAAFKRTEGYKYVGDLDRYREREILAAASLENTRQKVAEEVSVARSQMLSLEPE
                     LSMSGDEKAQMRHELLAEMAQERQQQEEYARRIQQSLARGMLNSKERDQGIER"
                     /name="MobA/MobL family protein"
     gene            854..1300
                     /locus_tag="FQC27_000360"
     CDS             854..1300
                     /locus_tag="FQC27_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="UAH92732.1"
                     /translation="MTNREDWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKTL
                     SALIRAEKTAEKAAAAKARVTAIIAAERKAAARAEQKARDHELYKAAGLMIVAGLVDSK
                     TGKPKFSAAELVGALAGIAELPRNHPKWQEWEKRGKELLTKDSA"
                     /name="conjugal transfer protein TraD"
     gene            complement(1494..2429)
                     /locus_tag="FQC27_000365"
     CDS             complement(1494..2429)
                     /locus_tag="FQC27_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UAH92733.1"
                     /translation="MSAALQYFEENLPRRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVANTHTPEIQAKRGRKGGIAKGEAYADKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            2930..3265
                     /locus_tag="FQC27_000370"
     CDS             2930..3265
                     /locus_tag="FQC27_000370"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAH92729.1"
                     /translation="MKRIVFSILLPLSFHANAYYLSCEQVGKIAVAVTTSSNEKATQND
                     TKYGDIQKNNLSKIENHAVLFCQEVRKFKVTPETADSWAEYTSLKAELDSEMKNNIKII
                     TRLSVIQ"
                     /name="hypothetical protein"
     gene            complement(3317..3640)
                     /locus_tag="FQC27_000375"
     CDS             complement(3317..3640)
                     /locus_tag="FQC27_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAH92730.1"
                     /translation="MTIIFNSDKQTDSESAFEKWLLHHPDGFVVNLRKAANGLSGKSDK
                     HKTFIHSASCHCLSSTKGGFTNGEYQKICSSSFEKAEALAKYMTGLDEIKRCSFCFGKD
                     KEC"
                     /name="hypothetical protein"
     Typing          complement(1495..2429)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.893"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3..682)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="4.19e-153"
     Typing          complement(3736..4529)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="7.67e-155"
ORIGIN
        1 gcccctcgtt cagattggcg gcgtgaacag ggccaagatg aacttccggc gtgcgcttga
       61 tgccctgttc tttcagcgaa cgatgatcta cgcgcgactc gtgaccgtgt cgcgcaaggt
      121 gctcattctg cacatcagcc cagcgggaac gtagcgcaac aagggcggct tttcgctctc
      181 cggcggtctg gggaatactc gccttttttg cgcctccccg ctccggtgat ttgctgttgg
      241 cgcgtttaaa aaattgctgc ggatcgcgtt caataccatc attaatccgc tcgcaaaaca
      301 tgatatgcgc gtgtggctgt tcctttccgt cgattgcacc tggcggattg tggatcgcgt
      361 actggtaggc atggcgattg ccaagttcct tttgcacgaa atcttcaaca agttctatcc
      421 gttgttcgcg tgtcagttca cgcggtaatg cgatctcaat ttcacggtat gccgtcccgt
      481 tttttcgttc aaattcatct gcggctttcc acagttctgc gggatttttc ttcgcccatg
      541 atggcatgtt accggatgac gaaaactcca gatcctcccg cgatttgtag ctcttatatt
      601 cgccggatct ggaaatgtaa tcagcatgtt ttaaggctgc gccttttgct ccggttttaa
      661 cggtacaatg gtagcttgcc atctgccttt tagccctccg caggagcgca cagtttcttg
      721 atgaaatcga gaaactacta agtgcgcatc cctgaataaa tcagggatga ttttacgttc
      781 tcacacgcag gcagacaagg tgatattatg cacaataaca actcacatta acggcaaaag
      841 tgcagaggta aggatgacaa atcgtgaaga ctggttaagt gcaaaaattg cctacatcaa
      901 cggactgaaa tcaccgtcag agcagcagag attacttgtg ttactggcag agaaaaaaaa
      961 ccgaacaacg acggatgaaa aaacgttgtc cgcactgata cgagcagaaa aaaccgcaga
     1021 gaaagcagcc gcagcaaaag ccagagtaac ggcaatcata gcggcagaac ggaaggcagc
     1081 cgcaagggcg gaacaaaagg cacgagatca tgaactctac aaggccgcag gcctcatgat
     1141 tgtggcagga ctggtggaca gcaaaaccgg aaaaccaaag tttagtgctg ccgaactggt
     1201 aggcgcactc gcaggaatcg cagagctacc gcgcaaccat ccaaaatggc aggaatggga
     1261 aaaacgcggg aaagagctac tcaccaaaga cagtgcgtaa gagcctccgt tcacgttccg
     1321 gttccctggt gcttcctcgc tcattcgcta gctacgctcg gtcatgagac tgtggcgagc
     1381 acgaccacct caatcatacg gcaagccgca ccattgctgg gctgaaagga gaaaaccgcc
     1441 ggaaagcccg ctgctcaaaa agacgccgaa aaggcgctgt tatctgatat aggctatttt
     1501 ccgcttttcc aattccgaat agttcgagtg gaaacctcca acatcgccgc gatagctttc
     1561 tgagaatatc cattctccag catacataga gccatgaaac gcttgtcagc gtaggcttcg
     1621 cctttagcga tgccaccttt cctgcctctc ttggcctgta tttctggcgt gtgcgtatta
     1681 gccacatact gtgcgaaagt ttccggcgtg aagttcctgt gcgtgtattt cgcaatgctc
     1741 ttgccaatag cccgacattc aggcggagaa agcggaacgg gaagcgatgc gttgtacatt
     1801 tcgacacgct gaatcacggc atcaagccac tgtgagaatg ctggccagcc ctgacgaatc
     1861 gccctgtaag cccatttgcg cgtcatttcg aacaggtggc agtttcgccc catcccgtaa
     1921 tgtttatcga tgctacgacg cgctgaggcg ctcaaatcga gataatcagc cagttcatcg
     1981 agggtatagg cttcctcgcg ccactccatc acctgccatt ccaggtggaa cggatttttg
     2041 caaatcagtc cgctgtagtt cacatcagca caaagtttct cacacagcga acgttcaacc
     2101 gctgcggcat atttcagcgc cttaaccgac gcatcaggcg cggttctcac ggcgatattg
     2161 agtgcataga gcaggtgagc gtgtccgttc acaggatttt taacggtgat gttcggcgcg
     2221 ggggcgttcc ggtcgctcca gtcaatcgct gccccctcgc ggtcaacgtc aaaaaccagc
     2281 cagaactgcg catgaggctg gttctgctgg atgtaccgcg caagcagcgc acgccctttg
     2341 ccggagatgc gaagaccaaa agcgagatca tccgtgtgat agggacggcg gggtaaattt
     2401 tcttcgaagt attgaagcgc ggcgctcatg tgttacgatt ccttccaggt tatcgaccga
     2461 catcgcccct gtattttcgt gacagtcttg gcggatagac acgaatttca ggggcgtttg
     2521 tttttcagtc gcttacacta cccccaacga caaaacctgt cgagtcttcc cgcaaacccg
     2581 atccatttac cataactcct gttgtgtgca ccccttaaaa cgccctcaga cgctttctga
     2641 cgcaaaaaac tcaaaaagac aagtaaacac caggcagaac cagaaaaacg cgtcagaatt
     2701 gtttctagga agctgcataa ctatgcacaa aagcgattcg cgaacagttt cgaccgttta
     2761 gaagggcgaa cgggtcaaaa cggcaaactt tccccatcag aaaacgcctc acagggagcg
     2821 aaatcttttc gctccctgtg aggcgttaga aaaatactaa gaattgcaaa atacgtattt
     2881 gtaaatgtag tatctacatg ttttttttat taaaaacgag acacctataa tgaaaagaat
     2941 agtattctca atattattgc cattgtcatt tcatgccaac gcgtactatt taagttgcga
     3001 gcaagttgga aaaatagcag tagcggtaac cacctcatca aatgaaaagg caactcaaaa
     3061 tgacacaaaa tatggagata ttcaaaaaaa caatttatca aaaatagaaa atcatgctgt
     3121 tttattttgc caagaagtta gaaaatttaa agtcactcca gaaactgccg atagctgggc
     3181 agaatataca tcccttaaag ctgaattaga ttcagaaatg aaaaacaaca taaaaattat
     3241 aacacgacta tctgtaatcc aataaataaa aagatcgcga taaaaatcgc gatcttacca
     3301 ataataaagt agcatattag cactctttat ctttaccaaa gcagaagctg caacgtttta
     3361 tctcatctag cccagtcata tatttagcta acgcctctgc tttctcaaaa gaagatgagc
     3421 atattttctg atattctcca ttagtaaatc cacctttcgt agaagagaga caatgacaac
     3481 tagcactgtg aataaaagtt ttatgtttat cacttttacc cgacaaacca ttagcagctt
     3541 ttctcaaatt aacaacaaag ccatcaggat ggtgaagcaa ccatttctcg aaagctgact
     3601 cgctatcagt ttgtttatcg ctgttaaaga taatagtcat ttaatttatc ttccatcaca
     3661 atcaatttca tctactcgtt aactattttt agttaattca gaaaaatttc tttaccagag
     3721 taaatacaac attatcgttc aataccctga tcacgctcct tactatttaa cattcctcta
     3781 gccaaagatt gctgtatacg ccgtgcgtat tcttcttgtt gctgcctttc ctgtgccatt
     3841 tctgccagta actcatgtcg catttgagct ttttcatctc cagacatact gagttcaggc
     3901 tccagagata acatctggct acgggcaacg gatacttctt cagcaacctt ttgcctggta
     3961 ttctctaaag atgccgcggc cagaatctca cgttctctgt acctgtccag atccccaacg
     4021 tacttatatc cctcggtacg cttaaaagca gcgttttctt tgtcccactc ggattcggct
     4081 tgctgtagct tgttcctgaa ttcattcctt tcatgttcag cggtttccag cacacttttt
     4141 tgctccagaa tacgcgcatc aatctccctt ttgtcagccc cccgaaacag gccacgaatc
     4201 ccattcaaag cattgcgttg ttcctgtagc tcccgcagct ccctttgtgc ggaaacaaca
     4261 ttacgcccca gtttgtctaa ataccgtgcc ccttcatata ttcgcctgct ccattcggaa
     4321 agagattctt ttttagcttt cgcattctga agcagcttat gttctttgag cgcatcgggg
     4381 ttggcatccc ctaaacgagc aagcctttcc tctccctgaa tgaccgtttt cataaccttg
     4441 cggtactgag ccagtaactc cggtgaacgc tcaactgtcc tgattttttg cttttgctcc
     4501 agttcctgtt ggcgctctgg cgctgggttg cgtgctgcgc gttcttgctg acgcgccgcc
     4561 ctgtgctcct gaattgccgc gatct
//
