LOCUS       CP082584               14459 bp    DNA     linear   BCT 13-JAN-2022
DEFINITION  Salmonella enterica subsp. enterica serovar Kentucky strain CVM
            N18S0935 plasmid pN18S0935-2.
ACCESSION   CP082584
VERSION     CP082584.1
DBLINK      BioProject: PRJNA292661
            BioSample: SAMN12586952
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Kentucky
  ORGANISM  Salmonella enterica subsp. enterica serovar Kentucky
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 14459)
  AUTHORS   Li,C., Tyson,G.H., Hsu,C.H., Harrison,L., Strain,E., Tran,T.T.,
            Tillman,G.E., Dessai,U., McDermott,P.F. and Zhao,S.
  TITLE     Long-Read Sequencing Reveals Evolution and Acquisition of
            Antimicrobial Resistance and Virulence Genes in Salmonella enterica
  JOURNAL   Front Microbiol 12, 777817 (2021)
   PUBMED   34867920
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14459)
  CONSRTM   NARMS: The National Antimicrobial Resistance Monitoring System
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2019) Center for Veterinary Medicine, US Food and
            Drug Administration, 8401 Muirkirk Rd, Laurel, MD, USA
REFERENCE   3  (bases 1 to 14459)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2020
            Assembly Method :: SMRTLink Microbial Assembly v. SMRTLink9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 329x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/30/2021 17:29:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,771
            CDSs (total)                      :: 4,648
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 37 of 100
            Pseudo Genes (incomplete)         :: 65 of 100
            Pseudo Genes (internal stop)      :: 20 of 100
            Pseudo Genes (multiple problems)  :: 19 of 100
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..14459
                     /organism="Salmonella enterica subsp. enterica serovar
                     Kentucky"
                     /mol_type="genomic DNA"
                     /strain="CVM N18S0935"
                     /serovar="Kentucky"
                     /isolation_source="Ground Turkey (Breasts)"
                     /sub_species="enterica"
                     /db_xref="taxon:192955"
                     /plasmid="pN18S0935-2"
                     /geo_loc_name="USA:LA"
                     /collection_date="2018"
                     /collected_by="FDA"
     gene            <1..265
                     /locus_tag="FWV45_023785"
     CDS             <1..265
                     /locus_tag="FWV45_023785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="UAI01512.1"
                     /translation="VGLTITLVSVLLIATSGSILWWQGQQITGNYQTIRAQERTQAMLS
                     EKNHGVQLSLCGEQKLSCVKVNPKAGAYGEEGNWMVWRGNSP"
                     /name="mobilization protein"
     gene            262..464
                     /locus_tag="FWV45_023790"
                     /pseudo=""
     CDS             262..464
                     /locus_tag="FWV45_023790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1143..2003)
                     /locus_tag="FWV45_023795"
     CDS             complement(1143..2003)
                     /locus_tag="FWV45_023795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="UAI01513.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="class A broad-spectrum beta-lactamase TEM-1"
     gene            complement(2186..2743)
                     /locus_tag="FWV45_023800"
     CDS             complement(2186..2743)
                     /locus_tag="FWV45_023800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="UAI01514.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            2907..5912
                     /locus_tag="FWV45_023805"
     CDS             2907..5912
                     /locus_tag="FWV45_023805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="UAI01515.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            6248..6658
                     /locus_tag="FWV45_023810"
     CDS             6248..6658
                     /locus_tag="FWV45_023810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAI01516.1"
                     /translation="MAFISLSNLAAKTGRIIQSQCVTATQTKKSTGAVELGFRLSKSAM
                     DETGITIGDRVDILYDKEENQWMIKKISEGGFGVSGQKNKHGGYSSCAVRLTLRPGFPR
                     LSEDEEDNKKLLSDDSFTQLSEGVIVFRLDDE"
                     /name="hypothetical protein"
     gene            6916..7479
                     /locus_tag="FWV45_023815"
     CDS             6916..7479
                     /locus_tag="FWV45_023815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAI01517.1"
                     /translation="MHALSILPSVARANLATKFSSHLKVIELFNTMQDSQVVVLSSLIE
                     GHHLTSSGNSVKADFEVTRLPAIIEMLEKKYFFPIRHLNVSVKSVTTGRMTGQTVYFIE
                     PEHIEQLLADPELVFANQERSLFFRSLEKEGKNLGKLIEKKGSVSQAVLSLLHHAYKDK
                     PLSDEMWKEIEDKFTHMLDELSAA"
                     /name="hypothetical protein"
     gene            complement(7508..7969)
                     /locus_tag="FWV45_023820"
     CDS             complement(7508..7969)
                     /locus_tag="FWV45_023820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAI01522.1"
                     /translation="MSKQDYIQLRTLTDQFYADNKGLQEALDGSNDGKVRGYGIVVIDL
                     NGLVFGIPLRSHLNHKFGFVSERSEGVKKGLDYTKALLIKKEEYVSRAYKIPTPEFTHI
                     NDNKEKIQEDFNKFVNRYIEANVKKDENILRNYRYSTLKNYHKELGLED"
                     /name="hypothetical protein"
     gene            9157..9477
                     /locus_tag="FWV45_023825"
     CDS             9157..9477
                     /locus_tag="FWV45_023825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UAI01518.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNTGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGND
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            9440..10180
                     /locus_tag="FWV45_023830"
                     /pseudo=""
     CDS             9440..10180
                     /locus_tag="FWV45_023830"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            10155..10640
                     /locus_tag="FWV45_023835"
     CDS             10155..10640
                     /locus_tag="FWV45_023835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UAI01519.1"
                     /translation="MSSLLALAKDLEKQSKAQQQNTCEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEQKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            10644..10856
                     /locus_tag="FWV45_023840"
     CDS             10644..10856
                     /locus_tag="FWV45_023840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UAI01520.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(11527..12387)
                     /locus_tag="FWV45_023845"
     CDS             complement(11527..12387)
                     /locus_tag="FWV45_023845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="UAI01521.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="class A broad-spectrum beta-lactamase TEM-1"
     gene            complement(12570..13148)
                     /locus_tag="FWV45_023850"
     CDS             complement(12570..13148)
                     /locus_tag="FWV45_023850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="UAI01523.1"
                     /translation="MRFKVHFYATFGYARVSTVQCSLDLQVRALKDAGVKANRIFTDKA
                     SGSSTDREGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGI
                     STDGDMGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQ
                     KGTGATEIAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            13290..14459
                     /locus_tag="FWV45_023855"
                     /pseudo=""
     CDS             13290..14459
                     /locus_tag="FWV45_023855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     ARG             complement(11531..12387)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(1147..2003)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          8870..9049
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.792"
                     /coverage="83.0"
                     /evalue="3.5099999999999995e-46"
     Typing          complement(8411..8924)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.217"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          9468..10972
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.426"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgtcgggctg accatcaccc tggtctcagt actgctgatc gccacgagcg ggagcattct
       61 gtggtggcag gggcagcaga tcaccgggaa ttaccagact atccgggcgc aggagcggac
      121 gcaggccatg ctgtcggaga agaaccacgg cgtgcagctg tcgctctgtg gggaacagaa
      181 actgtcctgc gtcaaagtga acccgaaagc gggagcttac ggggaggaag ggaactggat
      241 ggtatggaga ggaaatagcc catgacggaa ctggaaaaac agttgctgac gcattagagc
      301 agctacatca ggactactcg caaaggctgg acgaggggag agcgccttcg cggaatggca
      361 gaagatgtct ggtcttatgc aacggcgaga acgcggcgct gaacgagcgc gtcacgcgct
      421 tgagtcagca ggtggcgaac ttgagcagca gttacagcgt ttgagccagt agtgcagcgg
      481 catgaaatgg cggtggccgc cgaacgggca tacgagcagc gacagcatga aaaagagctg
      541 gcagaacccc gtttccgtca gcggagctat aacggtccga cgctgggctg actgcgcgca
      601 tccagtgaca aaccccaaga agaagggctc ataaatttcc cgctaaatac ggtcgtgatg
      661 aaaagatcac ggctcaagag agggggattt atgaagatac taaaagcgct cagtctgata
      721 ctgcaatgcg tcaaactggc aatggatctg tgggagcgat tcgggggaaa gtggcctttt
      781 tagtgcttta caccgtattt atacgcgaaa actgctgtca ttcatctggc tgcataacca
      841 tgcagccaag tgaaatttat ctggcgtaaa cggggcggtt taccgggtgc tttgctaggg
      901 gtttcacccg acaggcaccg tatttgcgcc gaaccagccc gaagcggtgc gggcaagaac
      961 tcttatgtta aattcatctc atcggcaacc tatacggggt ctgacgctca gtggaacgaa
     1021 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1081 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1141 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1201 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1261 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     1321 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1381 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1441 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1501 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1561 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1621 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1681 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1741 tgctcttgcc cggcgtcaac acgggataat accgcaccac atagcagaac tttaaaagtg
     1801 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1861 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     1921 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     1981 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2041 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2101 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     2161 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attttataaa
     2221 ccgtggagcg ggcaatactg agctgatgag caatttccgt tgcaccagtg cccttctgat
     2281 gaagcgtcag cacgacgttc ctgtccacgg tacgcctgcg gccaaatttg attcctttca
     2341 gctttgcttc ctgtcggccc tcattcgtgc gctctaggat cctccggcgt tcagcctgtg
     2401 ccacagccga caggatggtg accaccattt gccccatatc accgtcggta ctgatcccgt
     2461 catcaatgaa ccggactgcc acgccctgag cgtcaaattc ctttatcagt tggatcatat
     2521 cggcagtgtc gcggccaaga cggtcgagct tcttaaccag aatgacatca ccttcctcca
     2581 ccttcatcct cagcaaatcc agcccttccc gatctgttga actgccggat gccttatcgg
     2641 taaatatacg gtttgctttc acacctgcgt ctttgagtgc tctgacctga agatcaagag
     2701 actgctgact ggttgagacc cgagcgtaac caaaaagtcg cataaaaatg taccttaaat
     2761 cgaatatcgg acaactcatg tctattatta caaatttacg atttaataga catattaatg
     2821 taacagtttt acgatgtccg ataatttata acatttcgta cggttgcaaa aatgttacta
     2881 aatgcccgtc aggcagggag gccgatatgc ccgttgactt tctgaccact gagcagactg
     2941 aaagctatgg cagattcacc ggtgaaccgg atgagcttca gctggcacga tattttcacc
     3001 ttgatgaagc agacaaggaa tttatcggaa aaagcagagg tgatcacaac cgtctgggca
     3061 ttgccctgca aattggatgt gtccgttttc tgggcacctt cctcaccgat atgaatcata
     3121 ttccttccgg cgtccggcat tttaccgcca gacagctcgg gattcgtgat atcaccgttc
     3181 ttgcagaata cggtcagagg gaaaataccc gccgtgagca tgcagcgctg atacgtcagc
     3241 actatcagta tcgtgaattt gcctggccct ggacatttcg ccttacccgt cttttatata
     3301 cccggagctg gataagcaac gaacgtcctg gcctgctttt cgatctggcg acagggtggc
     3361 ttatgcaaca tcgtattatt ctccccggag ccactacgct gacccggttg atttcagagg
     3421 taagggaaaa ggcgacgttg cgcctgtgga acaaactggc actgataccg tcagccgaac
     3481 agcgttcaca gctggagatg ctgctggggc caactgattg cagccgcctg tctttactgg
     3541 aatcactgaa aaagggccct gtgaccatca gtggtccggc gtttaatgaa gcaattgaac
     3601 gctggaaaac tctgaacgat tttggcctgc atgctgaaaa cctgagtaca ctcccggctg
     3661 tgcgcctgaa aaatctcgca cgttatgctg gtatgacttc ggtgttcaat attgccagga
     3721 tgtcaccgca gaaaaggatg gcggttctgg ttgcctttgt ccttgcatgg gaaacgctgg
     3781 cgctggatga tgcattggac gttctggacg ccatgctggc cgttatcatc cgtgacgcca
     3841 gaaagattgg gcagaaaaaa cggctccgct cgctgaagga tctggataaa tctgcattgg
     3901 cgctcgccag cgcatgttcg tacctgctga aagaagaaac accggacgaa tcgattcgtg
     3961 ctgaggtgtt cagctacatc ccaaggcaaa agctggctga aatcatcacg cttgtccgtg
     4021 aaattgcccg gccctcagac gataattttc atgaagaaat ggtggagcag tacgggcgcg
     4081 ttcgtcgttt cctgccccat ctgctgaata ccgttaaatt ttcatccgca cctgccgggg
     4141 ttaccactct gaatgcctgt gactacctca gccgggagtt cagctcacgg cggcagtttt
     4201 ttgacgacgc accaacggaa attatcagtc ggtcatggaa acggctggtg attaacaagg
     4261 aaaaacatat cacccgcagg ggatacacgc tctgctttct cagtaaactg caggatagtc
     4321 tgaggcggag ggatgtctac gttaccggca gtaaccggtg gggagatcct cgtgcaagat
     4381 tactacaggg tgctgactgg caggcaaacc ggattaaggt ttatcgttct ttggggcacc
     4441 cgacagaccc gcaggaagca ataaaatctc tgggtcatca gcttgatagt cgttacagac
     4501 aggttgctgc acgtctttgc gaaaatgagg ctgtcgaact cgatgtttct ggcccgaagc
     4561 cccggttgac aatttctccc ctcgccagtc ttgatgagcc ggacagtctg aaacgactga
     4621 gcaaaatgat cagtgatcta ctccctccgg tggatttaac ggagttgctg ctcgaaatta
     4681 acgcccatac cggatttgct gatgagtttt tccatgctag tgaagccagt gccagagttg
     4741 atgatctgcc cgtcagcatc agcgccgtgc tgatggctga agcctgcaat atcggtctgg
     4801 aaccactgat cagatcaaat gttcctgcac tgacccgaca ccggctgaac tggacaaaag
     4861 cgaactatct gcgggctgaa actatcacca gcgctaatgc cagactggtt gattttcagg
     4921 caacgctgcc actggcacag atatggggtg gaggagaagt ggcatctgca gatggaatgc
     4981 gctttgttac gccagtcaga acaatcaatg ccggaccgaa ccgcaaatac tttggtaata
     5041 acagagggat cacctggtac aactttgtgt ccgatcagta ttccggcttt catggcatcg
     5101 ttataccggg gacgctgagg gactctatct ttgtgctgga aggtcttctg gaacaggaga
     5161 ccgggctgaa tccaaccgaa attatgaccg atacagcagg tgccagcgaa cttgtctttg
     5221 gccttttctg gctgctggga taccagtttt ctccacgcct ggctgatgcc ggtgcttcgg
     5281 ttttctggcg aatggaccat gatgccgact atggcgtgct gaatgatatt gccagagggc
     5341 aatcagatcc ccgaaaaata gtccttcagt gggacgaaat gatccggacc gctggctccc
     5401 tgaagctggg caaagtacag gcttcagtgc tggtccgttc attgctgaaa agtgaacgtc
     5461 cttccggact gactcaggca atcattgaag tggggcgcat caacaaaacg ctgtatctgc
     5521 ttaattatat tgatgatgaa gattaccgcc ggcgcattct gacccagctt aatcggggag
     5581 aaagtcgcca tgccgttgcc agagccatct gtcacggtca aaaaggtgag ataagaaaac
     5641 gatataccga cggtcaggaa gatcaactgg gcgcactggg gctggtcact aacgccgtcg
     5701 tgttatggaa cactatttat atgcaggcag ccctggatca tctccgggcg cagggtgaaa
     5761 cactgaatga tgaagatatc gcacgcctct ccccgctttg ccacggacat atcaatatgc
     5821 tcggccatta ttccttcacg ctggcagaac tggtgaccaa aggacatctg agaccattaa
     5881 aagaggcgtc agaggcagaa aacgttgctt aacgtgagtt ttcgttccac tgagcgtcag
     5941 acccctatac aggcataaaa atcagtttct ataagcggct ttcttgcacc tacttggtga
     6001 ttttttggat gcgctgacgt tgctcgactt taaatcacca tagaaaaata ccctttactt
     6061 acataaagat cgatctggat gtgtatcttg acgaggttca atacgagcaa ttgtttttat
     6121 atttaagatt tcttatcgcc gttttttgcg gctctagaaa aatcaataca aaagatcttg
     6181 aaagccacct cgctccctgg gtacacttcc gtataagaac tgatcgatgt ctaaaacgga
     6241 ttgtattatg gcttttatct ctctatcgaa cttggcagcg aagactggaa ggataatcca
     6301 gtcgcagtgt gttacggcca cgcaaacgaa aaaaagcacg ggcgctgtcg agctgggatt
     6361 ccgcttgagc aagagcgcta tggacgagac tggtattacc attggggatc gtgtcgacat
     6421 tctttacgac aaagaagaaa accagtggat gattaagaaa attagtgaag gtggctttgg
     6481 tgtgtctggg caaaaaaaca agcacggcgg ttactcatcg tgtgctgtta gattgacctt
     6541 gcgaccaggt tttcctcgcc tttctgagga tgaagaggac aacaagaagc tactttccga
     6601 tgattcattc actcagctaa gtgaaggtgt aatcgtcttc aggctggatg atgaatagga
     6661 aggaccaaaa aggaaaggca gagttagtga gcgtcgccaa acaatcgact aactccgcct
     6721 tcttgtcaac acatcactac ctttggaagg gtggagtgtt atgggcggca ttatgttcat
     6781 aaacttgcat tctaataaag aaaaaatcgt ttcttttgag ggatgtgaac acgacatttt
     6841 ctattctgat aggtgcgatg ctattggctt tggtctctta agtttatgtt tttaaaaaca
     6901 tatgaggcta tacatatgca cgctttatca atactacctt cagttgcccg cgctaacctg
     6961 gcaactaaat tctcgtctca tttgaaggtg atcgagctct tcaacacgat gcaggattcc
     7021 caagttgttg tcttgtcatc actgatcgaa ggccatcacc tcaccagttc tggtaacagt
     7081 gtcaaagccg actttgaggt cactcgctta ccggcgatta tcgagatgtt agagaaaaaa
     7141 tattttttcc ctattcgtca tctaaatgta tctgttaaat ctgtaactac aggaaggatg
     7201 actggacaaa ctgtctattt catagagcct gaacacatcg agcaacttct cgctgatcct
     7261 gagcttgtgt ttgctaatca ggaaaggagc ctcttctttc gttctctcga aaaagaaggg
     7321 aaaaatctcg gcaagcttat cgagaaaaaa ggtagtgttt ctcaagccgt cttaagcctt
     7381 ctacatcacg cctacaaaga taaacctctt tctgacgaga tgtggaagga gatagaagat
     7441 aagttcaccc atatgctgga tgaattgagc gcggcgtaaa ggcaagggcg gtcgttagac
     7501 cgccttttta gtcttctaaa cctaattctt tgtgatagtt tttcagcgta gagtaacgat
     7561 agtttctgag tatgttttca tctttcttga cgttcgcttc tatgtatctg tttacgaatt
     7621 tgttgaaatc ttcttgtatt ttttctttgt tatcattgat atgggtaaat tcaggagttg
     7681 gtatcttata ggctcgcgat acatactctt ctttcttaat tagcaacgct ttggtgtaat
     7741 ccagtccttt cttaacacct tctgatcgct ccgaaacgaa tccaaattta tggtttaaat
     7801 ggcttcgtaa gggtatcccg aacaccaatc cgttgagatc aataactacg attccgtagc
     7861 ctcttacttt tccatcatta ctgccgtcga gagcctcttg taggcctttg ttatcagcat
     7921 aaaactggtc tgtaagggtt cttaactgta tgtaatcctg tttgctcatt tttgatgtcc
     7981 attggcattt tcctgtaacc aatgtacaaa aaaagcaaag ccctctgtaa gagagggctt
     8041 tgcttttctt tcgttgagtc ttggattttt tacgtgtggc gaactctacc gcacatcttt
     8101 ctttggaagc ttgtttgttt aacgtggggc tcattcctac cgccacttct agatttactt
     8161 atatcaaaaa ttatatgtga agtcaatttc gtagctgttc ttgactggac ttactttgcc
     8221 agcgtaatct tctcctgaaa cgaaaaaacc accctggcag gtggtttttc gaaggttatt
     8281 taatcctggc agattatcta accgtggtaa cagtcttgtg cgagacgtgt caccaaatcc
     8341 gtccttccag tgtagcctca gtaaggccac cacttcaaga actctcaata catctctcgc
     8401 acatcatgtt taccagtggc cgttgccaat ggcgattagt cgtgtctttc cgggttggac
     8461 tcaagtcgat agttaccgga aaaggcgcag cggtcggact gaacgggggg ttcgtgcata
     8521 cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagataa
     8581 acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac
     8641 gagggagcca ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca
     8701 ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg
     8761 cagcgccgca tccttttctt gccctgtttg tcatgccgta tgcctttgct cttatccgtg
     8821 tgcaggtaac gcccgccgca gccgaacggc aggagcggaa gcgaccgagt gagcgaggag
     8881 gcgtaatatc accgcaacct ccatgttctc ccgtacgccc tgcagctgca ttttcccctg
     8941 ccacatgaag cacttttata aagtgttact cctccgtaac atattaagcc agtatacact
     9001 ccgctagcgc tacgtgactg gttcagggct gcgccccgaa acccgctaaa agccactgac
     9061 gcgcctgcgg cttgtccaac accgtgccga ccgggaaaca tttcccgccc gtcccggtgt
     9121 tatcaggagg ctactctggc agacaaacgc aacaagatgc tcacgatgtg ggtgacggaa
     9181 gacgaacacc ggcggctgct cgagcggtgc gacggcaagc agctggcggc gtggatgcgt
     9241 cagacctgcc tcgatgagaa gccggcgcgt gccggcagac tgccgtcgat atcgccggcg
     9301 ctgctgcgcc agcttgccgg tatgggtaat aacctgaacc agatcgcccg gaaagtgaat
     9361 accggtggtg ccggtcatga ccgggtgcag atcgtggccg ccctgatggc aatcgacgcg
     9421 ggactggagc ggctgcggca tgcggtgctg gaaaaagggg ggaatgatga tcgttaaatt
     9481 ccatccccgg gggcgcggtg gtggtgccgg tccggtggat tacctgctgg gaaaagaccg
     9541 gcagcgcgag ggggcgaccg tgctgcaggg aaagccggag gaagtccggg agcttatcga
     9601 tgcctcgccg tatgcgaaaa agtacacctc cggcgtactg tcgttcgcgg aggctgagct
     9661 gccgcccggg cagcgggagc agatcatggc cagctttgag cgggttctga tgcccgggct
     9721 cgataaagac cagtacagca ttctgtgggt ggagcacacc gacaaggggc ggctggagct
     9781 gaatttcctg atccccaaca cagagctgct gaccgggaaa cgcctgcagc cgtattacga
     9841 tcgcgccgac cggccgcgca tcgatgcctg gcagaccgtg gtgaacggca gactggggct
     9901 gcatgatcca aacgccccga agaaccggcg gttactggtc acgccgtccg cactgccgga
     9961 aacgaagctg gaagccgctc aggcgattac acggggtttg ctggccttag cctcatccgg
    10021 ggcgctcaaa acgcgcgagg acgtcacagg gtcgctcact gcggcaggtt ttgaggtggt
    10081 ccgcaccaca aagaacagta tcagcattgc cgacccggac ggggggcgaa acatccgact
    10141 gaagggagcc atttatgagc agtcttttag cgctggcgaa ggacttggaa aagcaatcga
    10201 aagcgcagca gcagaatacc tgcgagatgc tgaaagccgc attcagcgag cacgagaagt
    10261 ctgtaaaagc ggaactgagc gcaagcgcga agagaatcag cgacgccatc agcgcccacg
    10321 agcagggtat gaccgcggcg atgcagtcga accggctgag cgtgctgcgt atggttggcc
    10381 ggacgtggct gaccatcacc ctggtctcag tactgctgat cgccacgagc gggagcattc
    10441 tgtggtggca ggggcagcag atcaccggga attaccagac tatccgggcg caggagcgga
    10501 cgcaggccat gctgtcggag aagaaccacg gcgtgcagct gtcgctctgt ggggaacaga
    10561 aactgtcctg cgtcaaagtg aacccgaaag cgggagctta cggggaggaa gggaactgga
    10621 tggtactgga gaggaaatag cccatgacgg aactggaaaa acagttgctg accgcattag
    10681 agcagctaca tcaggactac tcgcaaaggc tggacgagtg ggagagcgcc ttcgcggaat
    10741 ggcagaagat gtctggtctt atgcaacggg agaacgcggc gctgaacgag cgcgtcacgc
    10801 gcttgagtca gcaggtggcg aacttgagca ggcagttaca gcgtttgagc cagtagtgca
    10861 gcggcatgaa atggcggtgg ccgccgaacg ggcatacgag cagcgacagc atgaaaaaga
    10921 gctggcagaa ccccgtttcc gtcagcggag ctataacggt ccgacgctgg gctgactgcg
    10981 cgcatccagt gacaaacccc aagaagaagg gctcataaat ttcccgctaa atacggtcgt
    11041 gatgaaaaga tcacggctca agagaggggg atttatgaag atactaaaag cgctcagtct
    11101 gatactgcaa tgcgtcaaac tggcaatgga tctgtgggag cgattcgggg gaaagtggcc
    11161 tttttagtgc tttacaccgt atttatacgc gaaaactgct gtcattcatc tggctgcata
    11221 accatgcagc caagtgaaat ttatctggcg taaacggggc ggtttaccgg gtgctttgct
    11281 aggggtttca cccgacaggc accgtatttg cgccgaacca gcccgaagcg gtgcgggcaa
    11341 gaactcttat gttaaattca tctcatcggc aacctatacg gggtctgacg ctcagtggaa
    11401 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    11461 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    11521 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    11581 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    11641 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    11701 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    11761 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    11821 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    11881 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    11941 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    12001 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    12061 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    12121 gagttgctct tgcccggcgt caacacggga taataccgca ccacatagca gaactttaaa
    12181 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    12241 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    12301 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    12361 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    12421 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    12481 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
    12541 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaatttta
    12601 taaaccgtgg agcgggcaat actgagctga tgagcaattt ccgttgcacc agtgcccttc
    12661 tgatgaagcg tcagcacgac gttcctgtcc acggtacgcc tgcggccaaa tttgattcct
    12721 ttcagctttg cttcctgtcg gccctcattc gtgcgctcta ggatcctccg gcgttcagcc
    12781 tgtgccacag ccgacaggat ggtgaccacc atttgcccca tatcaccgtc ggtactgatc
    12841 ccgtcatcaa tgaaccggac tgccacgccc tgagcgtcaa attcctttat cagttggatc
    12901 atatcggcag tgtcgcggcc aagacggtcg agcttcttaa ccagaatgac atcaccttcc
    12961 tccaccttca tcctcagcaa atccagccct tcccgatctg ttgaactgcc ggatgcctta
    13021 tcggtaaata tacggtttgc tttcacacct gcgtctttga gtgctctgac ctgaagatca
    13081 agactgcact ggactgttga gacccgagcg taaccaaaag tcgcataaaa atgtacctta
    13141 aatcgaatat cggacaactc atgtctatta ttacaaattt acgatttaat agacatatta
    13201 atgtaacagt tttacgatgt ccgataattt ataacatttc gtacggttgc aaaaatgtta
    13261 ctaaatgccc gtcaggcagg gaggccgata tgcccgttga ctttctgacc actgagcaga
    13321 ctgaaagcta tggcagattc accggtgaac cggatgagct tcagctggca cgatattttc
    13381 accttgatga agcagacaag gaattatcgg aaaaagcaga ggtgatcaca accgtctggg
    13441 cattgccctg caaattggat gtgtccgttt tctgggcacc ttcctcaccg atatgaatca
    13501 tattccttcc ggcgtccggc attttaccgc cagacagctc gggattcgtg atatcaccgt
    13561 tcttgcagaa tacggtcaga gggaaaatac ccgccgtgag catgcagcgc tgatacgtca
    13621 gcactatcag tatcgtgaat ttgcctggcc ctggacattt cgccttaccc gtcttttata
    13681 tacccggagc tggataagca acgaacgtcc tggcctgctt ttcgatctgg cgacaggtgg
    13741 cttatgcaac atcgttattc tccccggagc cactacgcac ccggttgatt tcagaggtaa
    13801 ggaaaaggcg acgttgcgcc tgtggaacaa actggcactg atacgtcagc cgaacagcgt
    13861 tcacagctgg agatgctgct gggccaactg attgcagccg cctgtcttta ctggaatcac
    13921 tgaaaaaggg ccctgtgacc atcagtggtc cggcgtttaa tgaagcaatt gaacgctgga
    13981 aactctgaac gattttggcc tgcatgctga aaaacctgag tacactcccg gctgtgcgcc
    14041 tgaaaaatct cgcacgttat gctgtatgac ttcggtgttt caatattgcc aggatgtcac
    14101 cgcagaaaag gatggcggtt ctggttgcct ttgtccttgc atgggaaacg ctggcgctgg
    14161 atgatgcatt ggacgttctg gacgccatgc tggccgttat catccgtgcg ccagaaagat
    14221 tgggcagaaa aaacggctcc gctcgctgaa ggatctggat aaatctgcat tggcgctcgc
    14281 cagcgcatgt tcgtacctgc tgaaagaaga aacaccggac gaatcgattc gtgctgaggt
    14341 gttcagctac atccaacaag gcaaaagctg gctgaaatca tcacgcttgt ccgtgaaatt
    14401 gcccggccct cagacgataa ttttcatgaa gaaatggtgg agcagtacgg gcgcgttcg
//
