LOCUS       CP082530               13949 bp    DNA     circular BCT 13-JAN-2022
DEFINITION  Salmonella enterica subsp. enterica serovar Hadar strain CVM
            N18S2154 plasmid pN18S2154-1, complete sequence.
ACCESSION   CP082530
VERSION     CP082530.1
DBLINK      BioProject: PRJNA292661
            BioSample: SAMN12587116
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Hadar
  ORGANISM  Salmonella enterica subsp. enterica serovar Hadar
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 13949)
  AUTHORS   Li,C., Tyson,G.H., Hsu,C.H., Harrison,L., Strain,E., Tran,T.T.,
            Tillman,G.E., Dessai,U., McDermott,P.F. and Zhao,S.
  TITLE     Long-Read Sequencing Reveals Evolution and Acquisition of
            Antimicrobial Resistance and Virulence Genes in Salmonella enterica
  JOURNAL   Front Microbiol 12, 777817 (2021)
   PUBMED   34867920
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13949)
  CONSRTM   NARMS: The National Antimicrobial Resistance Monitoring System
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2019) Center for Veterinary Medicine, US Food and
            Drug Administration, 8401 Muirkirk Rd, Laurel, MD, USA
REFERENCE   3  (bases 1 to 13949)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2020
            Assembly Method :: SMRTLink Microbial Assembly v. SMRTLink9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 446x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/30/2021 17:06:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,689
            CDSs (total)                      :: 4,564
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 19
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 44 of 97
            Pseudo Genes (incomplete)         :: 63 of 97
            Pseudo Genes (internal stop)      :: 18 of 97
            Pseudo Genes (multiple problems)  :: 26 of 97
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13949
                     /organism="Salmonella enterica subsp. enterica serovar
                     Hadar"
                     /mol_type="genomic DNA"
                     /strain="CVM N18S2154"
                     /serovar="Hadar"
                     /isolation_source="ground turkey"
                     /sub_species="enterica"
                     /db_xref="taxon:149385"
                     /plasmid="pN18S2154-1"
                     /geo_loc_name="USA:NM"
                     /collection_date="2018"
                     /collected_by="FDA"
     gene            join(12996..13949,1..677)
                     /locus_tag="FWW82_000340"
                     /pseudo=""
     CDS             join(12996..13949,1..677)
                     /locus_tag="FWW82_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            711..1316
                     /locus_tag="FWW82_000345"
                     /pseudo=""
     CDS             711..1316
                     /locus_tag="FWW82_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            2138..2332
                     /locus_tag="FWW82_000350"
     CDS             2138..2332
                     /locus_tag="FWW82_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UAI66368.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2986..3098
                     /locus_tag="FWW82_000355"
                     /pseudo=""
     CDS             2986..3098
                     /locus_tag="FWW82_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(3329..3625)
                     /locus_tag="FWW82_000360"
     CDS             complement(3329..3625)
                     /locus_tag="FWW82_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="UAI66369.1"
                     /translation="MINLVDPVHPGVFIREMFMEPFEISAADLSEKLHVSPSTLSRVLN
                     GKADLSVEMALRLSRVLGRSAESWINMQTQYSLNASRKSPNVIKELDELVPIF"
                     /name="HigA family addiction module antidote protein"
     gene            3970..4251
                     /locus_tag="FWW82_000365"
     CDS             3970..4251
                     /locus_tag="FWW82_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="UAI66370.1"
                     /translation="MIINIKHKGLRKFAETGNNAGIPQQFTQRLKLILTALSTATNLQD
                     MMDLKALRCHPLKGDRQGEYSLEVNGNWRVVFKLEEPNVFDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4759..5619)
                     /locus_tag="FWW82_000370"
     CDS             complement(4759..5619)
                     /locus_tag="FWW82_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="UAI66371.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="class A broad-spectrum beta-lactamase TEM-1"
     gene            complement(5802..6359)
                     /locus_tag="FWW82_000375"
     CDS             complement(5802..6359)
                     /locus_tag="FWW82_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="UAI66372.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            6521..9526
                     /locus_tag="FWW82_000380"
     CDS             6521..9526
                     /locus_tag="FWW82_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="UAI66373.1"
                     /translation="MSVDFLTTEQVESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            complement(9591..10241)
                     /gene="tetR(A)"
                     /locus_tag="FWW82_000385"
     CDS             complement(9591..10241)
                     /gene="tetR(A)"
                     /locus_tag="FWW82_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="UAI66374.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            10347..11546
                     /gene="tet(A)"
                     /locus_tag="FWW82_000390"
     ARG             10348..11543
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(11578..12462)
                     /locus_tag="FWW82_000395"
     CDS             complement(11578..12462)
                     /locus_tag="FWW82_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="UAI66366.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPA
                     GLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVV
                     FISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLT
                     RKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYF
                     LWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSN
                     RTPRARIACRKSP"
                     /name="EamA family transporter"
     gene            complement(12600..13007)
                     /locus_tag="FWW82_000400"
     CDS             complement(12600..13007)
                     /locus_tag="FWW82_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="UAI66367.1"
                     /translation="MRGRPVLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEG
                     LAIERGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYD
                     VRLVQDCCYDPDRDAHEALLRSGFGGRVQVV"
                     /name="cysteine hydrolase"
     ARG             complement(4763..5619)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(1624..1879)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="89.0"
                     /evalue="1.21e-116"
     Typing          complement(2004..2102)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          complement(2406..2488)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.79e-36"
     Typing          2501..3055
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.614"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtgaatgtcg gcgtccgcga ttccacctat gtgctcgacg gcctgctgta ccacgagtcc
       61 gacctgcgga tcgaggagca ctacaccgac acggccggct tcaccgatca cgtctttgcc
      121 ctgatgcacc tgctaggctt ccgcttcgcg ccgcgcatcc gcgacctcgg cgaaaccaag
      181 ctgtacgtgc cgcagggcgt gcaagcctac ccgacgttgc gcccgctgat cggcggcacc
      241 ctgaacatca agcacgtgcg tgcccactgg gccggatcga gcgcacgctg ttcatcctgg
      301 actggctgca aagtgttgaa ctgcgccgcc gcgtgcatgc cggcctgaac aaaggtgagg
      361 cgcgcaactc gctggccagg gcggtgttct tcaaccgcct tggggaaatc agggatcgga
      421 gcttcgagca gcagcgctac cgggccagcg gcctcaacct ggtgacggcg gctatcgtgc
      481 tgtggaacac ggtgtacctg gaacgcgcca cccaggggtt ggtcgaggcc ggcaagccgg
      541 tggacggcga gctgctgcaa ttcctgtcgc cgctgggctg ggagcacatc aacctaaccg
      601 gcgattacgt ctggcggcag agccgcagac tggaagacgg gaagtttcgg cccttacgga
      661 tgcccggaaa accttagcgt acgatttttt ccgaattctg cgggctcccc gactatggcg
      721 tgctgaatga tattgccaga gggcaatcag atccccgaaa aatagtcctt cagtgggacg
      781 aaatgatccg gaccgctggc tccctgaagc tgggcaaagt acaggtttca gtgctggtcc
      841 gttcattgct gaaaagtgaa cgtccttccg gactgactca ggcaatcatt gaagtggggc
      901 gcatcaacaa aacgctgtat ctgcttaatt atattgatga tgaagattac cgccggcgca
      961 ttctgaccca gcttaatcgg ggagaaagtc gccatgccgt tgccagagcc atctgtcacg
     1021 gtcaaaaagg tgagataaga aaacgatata ccgacggtca ggaagatcaa ctgggcacac
     1081 tggggctggt cactaacgcc gtcgtgttat ggaacactat ttatatgcag gcagccctgg
     1141 atcatctccg ggcgcagggt gaaacactga atgatgaaga tatcgcacgc ctctccccgc
     1201 tttgccacgg acatatcaat atgctcggcc attattcctt cacgctggca gaactggtga
     1261 ccaaaggaca tctgagacca ttaaaagagg cgtcagaggc agaaaacgtt gcttaacgtg
     1321 agttttcgtt ccactgagcg tcagacccct aaaaccatcc cgtaaatcac agcatttacc
     1381 ataacgtcgc attacgcgca ccgctaaaac gcgctgaacg cgattctgcc gcgaacaccg
     1441 caaaaagaga tgttttacca agcgaaaacc ggaaaaatgc gtcaggatcg ctttcagctc
     1501 actgcatagc tatgcatgaa agtgaatggc gatcggtttg ggcgcatacg gtgcccatga
     1561 gacacatttt cggacatttt cagttcgtga acccctgact cattgctaca tatctgaaca
     1621 gacaccccta gcagcgcccc caggggcgtc ctgtaaaaaa acacaccgcg ccgctagcag
     1681 cacccctaat ataaaatgat gttttttatg aaaatagtca gtaccacccc taaaaagcgg
     1741 tgttggcgca gctacaacac cgcgccgaca ccgctttttt aaatatcaca aagagagcaa
     1801 gaagaactaa tttgtcataa ctctctattt ataaagaaaa atcagcaaaa aattgttttt
     1861 gcgtggggtg tgggcatttc aggcatgaga acagactcgc gtttttccgg agtatcagcg
     1921 gaattattga ttaagaaaca ctcgacgcag agcaaaattt tatgcaacag cggctgcact
     1981 gcacagggat tatttatcag ttgatatgct gtgccatgat acgtcctaaa aaaacaggac
     2041 aggatgtgca attgtaatac cgtcgcccgc aacgtcttac aattaccacc tggtcagggt
     2101 ccacccgaca cccggtactg acggagaaca acggaaggtg aacaaacagc aacaaactgc
     2161 gctgaatatg gcgcgattta tcaaaagcca gagcctgaca ctgcttgaaa aactggatgc
     2221 tctggatgcc gacgaacagg cggccatgtg cgaacgactg cacgaactcg cggaagaact
     2281 ccagaacagc atccaggttc gctttgagac cgaaagtgaa acaggaacat aacgaagctg
     2341 acggaggcgc tcacggcttg tccgcgagcg ttttcggcag cagacaagcc cgccagggcg
     2401 cgtcagcggg tgtcggggcg aagccctgac ccagtcacgt agcgatagcg gagtgtatac
     2461 tggcttaact atgcggcatc agtgcagatt gtatgaaaag tgccccatgc cgggtgtgaa
     2521 attccgcaca gatgcgtaag gagaaaatgc gcgtccaggc gcttttccgc ttcctcgctc
     2581 actgactcgc tacgctcggt cgttcggctg cggcgagcgg tactgactta cacaaaaacg
     2641 gcagcacggt tatccacaga atcaggggat aaggccggaa agaacatgtg agcaaaagac
     2701 caagaacagg aagaaggccg cgccgcaggc gtttttccat aggctccgcc cccctgacga
     2761 gcatcacaga aatcgacgct caagtcagag gtggcgaaac ccgacaggac ttaaagatac
     2821 cagacgtttc cccctggtgg ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2881 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2941 aggtatctcg gttcagtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     3001 gttcagcccg accgctgcgc cttatccggt aactatcatc ttgagtccaa cccggaaaga
     3061 catgcaaaac accattggca gcagccactg gtaactgatt tagaggagtt agtcttgaag
     3121 ttatgcgcca gttaaggcta aactgaaaga acaagttttg gtgactgcgc tcctccaagc
     3181 cagttaccct ggttcagaaa cttgccggtt actgaacctt cgaaaaaacc accttgccgg
     3241 gtggtttttt cgtttacgag aaacgatacg atccagttgc ctgagagtca agacagaaaa
     3301 aggccgcata tagcggcctt ttcttttctc aaaatatagg aaccaattca tctaattcct
     3361 taatgacatt aggtgacttc ctgctcgcgt taagactgta ttgagtctgc atgtttatcc
     3421 acgactcggc actgcgacca agaactcgag aaagccttaa tgccatctca acacttaaat
     3481 cagcttttcc attcagcact ctacttagag tggaaggtga tacatgcagt ttttcagaca
     3541 ggtccgcggc tgatatttca aacggttcca taaacatctc acggataaaa acccctggat
     3601 gtactggatc gacaagatta atcatacctt cctctgaagc ctatattagt tttaaaaaga
     3661 taggcctatt gcgttttcag attgcgcatt gcgcataacg caactcattg ttgactaaaa
     3721 aaaagaatga cattatgatg cacattggtg cgtctaatca ttccttatga tgtgggtact
     3781 tgatgtgaaa ttcaaggccc cggcattgta cagcgcatca aaccaatgtc aacagattaa
     3841 aatgcgactt ggttaagtaa aacctacgtc atttattcaa atagagcaag gggtttgcaa
     3901 tggaaatacg gatgaaccgg aaaaataaac tgcgatgttt ctatctaaac aaatatggga
     3961 tggtgattaa tgatcataaa tatcaagcat aaaggtttgc gtaagtttgc cgaaacaggt
     4021 aacaacgcag gaattccgca acagtttact caacgtctga agctgatcct aacagcctta
     4081 agcacagcga cgaacttgca ggatatgatg gatttaaagg cattgcgctg ccatccattg
     4141 aagggagacc gtcaaggaga gtattcgtta gaggtcaatg gaaactggcg cgtagtgttt
     4201 aagctcgaag aacctaacgt ctttgacgtg gatctcgagg attatcacta agaaggtcct
     4261 taaagcatca aagtgacagt atcgatgctt tagggtctca taaaaaagca aaacctcggg
     4321 gtaagttata tgaagtattt tctgtaccag atagtcccta tagcagccat catttgcgca
     4381 gcaattctag cgctaaaagg tatcaatggc tgggggtggt tcttaactgt tgggttcctg
     4441 cttgcagggg cagatacagt aaaggctcag ttatctaagt agtaataaac actatcaata
     4501 gtggttggat aattgccaaa aaagcctcta tctgttctga aacagacaac aaggaaaaat
     4561 cggtgtaagc cacctccata agaggtggta ttgatatcaa catagataaa aggggtctga
     4621 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4681 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4741 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4801 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4861 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     4921 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4981 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5041 agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc
     5101 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5161 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5221 ggcagcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5281 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5341 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg caccacatag
     5401 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5461 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5521 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5581 aaagggaata agggcgacac gaaaatgttg aatactcata ctcttccttt ttcaatatta
     5641 ttgaagcatt taccagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5701 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     5761 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     5821 tcaagaattt tataaaccgt ggagcgggca atactgagct gatgagcaat ttccgttgca
     5881 ccagtgccct tctgatgaag cgtcagcacg acgttcctgt ccacggtacg cctgcggcca
     5941 aatttgattc ctttcagctt tgcttcctgt cggccctcat tcgtgcgttc taggatcctc
     6001 cggcgttcag cctgtgccac agccgacagg atggtgacca ccatttgccc catatcaccg
     6061 tcggtactga tcccgtcatc aatgaaccgg actgccacgc cctgagcgtc aaattccttt
     6121 atcagttgga tcatatcggc agtgtcgcgg ccaagacggt cgagcttctt aaccagaatg
     6181 acatcacctt cctccacctt catcctcagc aaatccagcc cttcccggtc tgttgaactg
     6241 ccggatgcct tatcggtaaa tatacggttt gctttcacac ctgcgtcttt gagtgctctg
     6301 acctgaagat caagagactg ctgactggtt gagacccgag cgtaaccaaa aagtcgcata
     6361 aaaatgtacc ttaaatcgaa tatcggacaa ctcatgtcta ttattacaaa tttacgattt
     6421 aatagacata ttaatgtaac agttttacga tgtccgataa tttataatat ttcgaacggt
     6481 tgcagttgtg ttaaaaaagc cgtcaggcag ggaggccgat atgtccgttg attttttgac
     6541 cactgagcag gttgagagtt atggcaggtt cactggcgaa cccgatgaac ttcagctggc
     6601 gcgttatttt catcttgatg aagcggataa agaatttatc gggaaaagcc ggggtgatca
     6661 caatcgactt ggtattgccc tgcaaatcgg gtgtgtgcgt tttctgggca cttttcttac
     6721 tgacatgaat catattcctt ccggcgtccg gcattttacc gccagacagc tcgggattcg
     6781 tgatatcacc gttcttgcag aatacggtca gagggaaaat acccgccgtg agcatgcagc
     6841 gctgatacgt cagcactatc agtatcgtga atttgcctgg ccctggacat ttcgccttac
     6901 ccgtctttta tatacccgga gctggataag caacgaacgt cctggcctgc ttttcgacct
     6961 ggcgacaggg tggcttatgc aacatcgtat tattctcccc ggagccacca cgctgacccg
     7021 gttgatttca gaggtaaggg aaaaggcgac gttgcgcctg tggaacaaac tggcactgat
     7081 accgtcagcc gaacagcgtt cacagctgga gatgctgctg gggccaactg attgcagccg
     7141 cctgtcttta ctggaatcac tgaaaaaagg ccctgtgacc atcagtggtc cggcgtttaa
     7201 tgaagcaatt gaacgctgga aaactctgaa cgattttggc ctgcatgctg aaaacctgag
     7261 tacactcccg gctgtgcgcc tgaaaaatct cgcacgttat gctggtatga cttcggtgtt
     7321 caatattgcc aggatgtcac cgcagaaaag gatggcggtt ctggttgcct ttgtccttgc
     7381 atgggaaacg ctggcgctgg atgatgcact ggacgttctg gacgccatgc tggccgttat
     7441 catccgtgac gccagaaaga ttgggcagaa aaaacggctc cgctcgctga aggatctgga
     7501 taaatctgca ttggcgctcg ccagcgcatg ttcgtacttg ctgaaagaag aaacaccgga
     7561 cgaatcgatt cgtgctgagg tgttcagcta catccctagg caaaagctgg ctgaaatcat
     7621 cacgcttgtc cgtgaaattg cccggccctc agacgataat tttcatgacg aaatggtgga
     7681 gcagtacggg cgcgttcgtc gtttcctgcc ccatctgctg aataccgtta aattttcatc
     7741 cgcacctgcc ggggttacca ctctgaatgc ctgtgactac ctcagccggg agttcagctc
     7801 acggcggcag ttttttgacg acgcaccaac ggaaatcatc agtcagtcat ggaaacggct
     7861 ggtgattaac aaggaaaaac atatcacccg aaggggatac acgctctgct ttctcagtaa
     7921 actgcaggat agtctgagac ggagggatgt ctacgttacc ggcagtaacc ggtggggaga
     7981 tcctcgtgca agattactac agggtgctga ctggcaggca aatcggatta aggtttatcg
     8041 ttctttgggg cacccgacag acccgcagga agcaataaaa tctctgggcc atcagcttga
     8101 tagtcgttac agacaggttg ctgcacgtct tggcgaaaat gaggctgtcg aactcgatgt
     8161 ttctggcccg aagccccggt tgacaatttc tcccctcgcc agtcttgatg agccggacag
     8221 tctgaaacga ctgagcaaaa tgatcagtga tctgctccct ccggtggatt taacggagtt
     8281 gctgctcgaa attaacgccc ataccggatt tgctgatgag tttttccatg ccagtgaagc
     8341 cagtgccaga gttgatgatc tgcccgtcag catcagcgcc gtgctgatgg ctgaagcctg
     8401 caatatcggt ctggaaccac tgatcagatc aaatgttcct gcactgaccc gacaccggct
     8461 gaactggaca aaagcgaact atctgcgggc tgaaactatc accagcgcta atgccagact
     8521 ggttgatttt caggcaacgc tgccactggc acagatatgg ggtggaggag aagtggcatc
     8581 tgcagatgga atgcgctttg ttacgccagt cagaacaatc aatgccggac cgaaccgcaa
     8641 atactttggt aataacagag ggatcacctg gtacaacttt gtgtccgatc agtattccgg
     8701 ctttcatggc atcgttatac cggggacgct gagggactct atctttgtgc tggaaggcct
     8761 tctggaacag gagaccgggc tgaatccaac cgaaattatg accgatacgg caggtgccag
     8821 cgatcttgtc tttggccttt tctggctgct gggataccag ttttctccac gcctggctga
     8881 tgccggtgct tcggttttct ggcgaatgga ccatgatgcc gactatggcg tgctgaatga
     8941 tattgccaga gggcaatcag atccccgaaa aatagtcctt cagtgggacg aaatgatccg
     9001 gaccgcaggc tccctgaagc tgggcaaagt acaggcctca gtgctggtcc gttcattgct
     9061 gaaaagtgaa cgtccctccg gactgactca ggcaatcatt gaagtggggc gcatcaacaa
     9121 aacgctgtat ctgcttaatt atattgatga tgaagattac cgccggcgca ttctgaccca
     9181 gcttaatcgg ggagaaagcc gtcatgcagt tgccagagcc atctgtcacg gtcaaaaagg
     9241 tgagataaga aaacgatata ccgacggtca ggaagatcag ttgggagctc tggggctggt
     9301 cactaacgcc gtcgtgttat ggaacactat ttatatgcag gcagctctgg atcatctccg
     9361 ggcgcagggt gaaacactga atgatgaaga tatcgcacgc ctctccccgc tttgccacgg
     9421 acatatcaat atgctcggcc attattcctt cacgctggca gaactggtga ccaaaggtca
     9481 tctgagacca ttaaaagagg cgtcagaggc agaaaacgtt gcttaacgtg agttttcgtt
     9541 ccactgagcg tcagacccct gagtgggttg cgctccggca gcggtcctga tcaatcgtca
     9601 ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa tccatcgaca
     9661 atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa ggcgtctatc
     9721 gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg ctcgccggca
     9781 tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct gattgtcatc
     9841 agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg aagctgcgcg
     9901 tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg cgcgccatcg
     9961 cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa tgagcgccag
    10021 tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc ttcggccagt
    10081 gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg ctgaaccccc
    10141 aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt caacaggtcc
    10201 agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac tttatcactg
    10261 ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt caatcggacc
    10321 agcggaggct ggtccggagg ccagacgtga aacccaacag acccctgatc gtaattctga
    10381 gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg ccgggcctcc
    10441 tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg ctggcgctgt
    10501 atgcgttgat gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat cgtttcgggc
    10561 ggcggccggt cttgctcgtc tcgctggccg gcgctgctgt cgactacgcc atcatggcga
    10621 cggcgccttt cctttgggtt ctctatatcg ggcggatcgt ggccggcatc accggggcga
    10681 ctggggcggt agccggcgct tatattgccg atatcactga tggcgatgag cgcgcgcggc
    10741 acttcggctt catgagcgcc tgtttcgggt tcgggatggt cgcgggacct gtgctcggtg
    10801 ggctgatggg cggtttctcc ccccacgctc cgttcttcgc cgcggcagcc ttgaacggcc
    10861 tcaatttcct gacgggctgt ttccttttgc cggagtcgca caaaggcgaa cgccggccgt
    10921 tacgccggga ggctctcaac ccgctcgctt cgttccggtg ggcccggggc atgaccgtcg
    10981 tcgccgccct gatggcggtc ttcttcatca tgcaacttgt cggacaggtg ccggccgcgc
    11041 tttgggtcat tttcggcgag gatcgctttc actgggacgc gaccacgatc ggcatttcgc
    11101 ttgccgcatt tggcattctg cattcactcg cccaggcaat gatcaccggc cctgtagccg
    11161 cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat tgccgacggc acaggctaca
    11221 tcctgcttgc cttcgcgaca cggggatgga tggcgttccc gatcatggtc ctgcttgctt
    11281 cgggtggcat cggaatgccg gcgctgcaag caatgttgtc caggcaggtg gatgaggaac
    11341 gtcaggggca gctgcaaggc tcactggcgg cgctcaccag cctgacctcg atcgtcggac
    11401 ccctcctctt cacggcgatc tatgcggctt ctataacaac gtggaacggg tgggcatgga
    11461 ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct gcgtcgcggg ctttggagcg
    11521 gcgcagggca acgagccgat cgctgatcgt ggaaacgata ggcctatgcc atgcgggtca
    11581 aggcgacttc cggcaagcta tacgcgccct aggagtgcgg ttggaacgtt ggcccagcca
    11641 gatactcccg atcacgagca ggacgccgat gatttgaagc gcactcagcg tctgatccaa
    11701 gaacaaccat cctagcaaca cggcggtccc cgggctgaga aagcccagta aggaaacaac
    11761 tgtaggttcg agtcgcgaga tcccccggaa ccaaaggaag taggttaaac ccgctccgat
    11821 caggccgagc cacgccaggc cgagaacatt ggttcctgta ggcatcggga ttggcggatc
    11881 aaacactaaa gctactggaa cgagcagaag tcctccggcc gccagttgcc aggcggtaaa
    11941 ggtgagcaga ggcacgggag gttgccactt gcgggtcagc acggttccga acgccatgga
    12001 aaccgccccc gccaggcccg ctgcgacgcc gacaggatct agcgctgcgt ttggtgtcaa
    12061 caccaacagc gccacgcccg cagttccgca aatagccccc aggaccgcca tcaatcgtat
    12121 cgggctacct agcagagcgg cagagatgaa cacgaccatc agcggctgca cagcgcctac
    12181 cgtcgccgcg accccgcccg gcaggcggta gaccgaaata aacaacaagc tccagaatag
    12241 cgaaatatta agtgcgccga ggatgaagat gcgcatccac cagattcccg ttggaatctg
    12301 tcggacgatc atcacgagca ataaacccgc cggcaacgcc cgcagcatcg cgaccgtcat
    12361 cggtgagaag ttcggcaggt attgggtggt gacaatgtag gtgctgcccc aaatggcagg
    12421 tgctatcgct gtgaacaata aatcgggcgt gcgtaatgac atgcgattac aagacctccg
    12481 ctacggcgat gggtttttga gagttgcagg cgccgcgatg accgcaccct gacttggact
    12541 ggccctgcct cgcaccaatc cacctgcggc tatggccgcg atcatgcgag gcccgaaaat
    12601 tacacgacct gtacacgtcc gccgaacccg gaacgcaaca gagcttcgtg ggcatcccga
    12661 tccgggtcgt agcagcagtc ctggaccaaa cgcacgtcgt agtccgcatc actagcccag
    12721 gcgacgcttg aaagaacaac gccggtggtg cttatcccgg ccatgacaag cgtgcttacg
    12781 ccgcgcgtcc gaaggtcggc gtcgagcgcc gtgccataaa agacgctggc tcgcggacat
    12841 gcatagaaaa ggtcgccccg ttcgatcgca agcccttcga cgggcagacc ggtgcgaaat
    12901 cgtccgctcg ggagatacgg tgagatttgg cgattcgtcg ccggcggcgc atgttcatac
    12961 tcttcgccga gcgaaaagtt ggggaacagc acgggccggc cgcgcaaggc gtgctggcag
    13021 ccgtgcagac cctgcgtgag atgaacgccg acaacctgcg caaggtgccg gccgatgcac
    13081 ccacggcctt catcaagccg cgctggaagc cgctggtgat caccccggaa ggcctcgacc
    13141 ggaaattcta cgaaatctgc gccctgtccg agctgaagaa cgccctgcgc tccggcgaca
    13201 tctgggtcaa gggctcgcgg cagttccgcg acttcgacga ctacctgctg ccggccgaga
    13261 agttcgccgc actcaagcgc gagcaggccc tgcccctggc gatcaacccg aacagcgacc
    13321 agtacctgga agagcgtttg cagctgctgg acgagcagtt ggccaccgtc acccgcctgg
    13381 ccaaggacaa cgagctgccc gatgccatcc tcaccgagtc agggctgaaa atcaccccgc
    13441 tggatgcggc ggtgccggat cgggcgcagg cgctgatcga ccaaaccagc cagttactgc
    13501 cgcgcatcaa gatcaccgaa ctgctgatgg acgtggacga ctggacgggc ttcagccgcc
    13561 acttcaccca cttgaaggac ggggccgagg ccaaagacag gacgttgctg ctgtccgcaa
    13621 tcctcggtga tgcgatcaac ctcgggctga ccaagatggc cgagtcgagc cccggcctga
    13681 cctacgccaa gctgtcctgg ctgcaagcct ggcacatccg cgacgaaacc tattcggcgg
    13741 ccttggccga gctggtcaac caccagtatc gccacgcctt tgccgcccac tggggcgacg
    13801 gcacgacctc atcctccgat ggccagcgct tccgcgcggg tggccggggc gagagcaccg
    13861 ggcacgtcaa cccgaagtac ggtagcgagc cgggacggct gttctatacc catatctccg
    13921 accagtacgc gccgttcagc acccgcgtg
//
