LOCUS       CP082512                4418 bp    DNA     circular BCT 12-JAN-2022
DEFINITION  Salmonella enterica subsp. enterica serovar Ohio strain FSIS1606132
            plasmid pF18S005-2, complete sequence.
ACCESSION   CP082512
VERSION     CP082512.1
DBLINK      BioProject: PRJNA292666
            BioSample: SAMN04600712
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Ohio
  ORGANISM  Salmonella enterica subsp. enterica serovar Ohio
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Li,C., Tyson,G.H., Hsu,C.H., Harrison,L., Strain,E., Tran,T.T.,
            Tillman,G.E., Dessai,U., McDermott,P.F. and Zhao,S.
  TITLE     Long-Read Sequencing Reveals Evolution and Acquisition of
            Antimicrobial Resistance and Virulence Genes in Salmonella enterica
  JOURNAL   Front Microbiol 12, 777817 (2021)
   PUBMED   34867920
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2020
            Assembly Method :: SMRTLink Microbial Assembly v. SMRTLink9.0
            Assembly Name                     :: F18S005
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 214x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/02/2021 14:10:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,781
            CDSs (total)                      :: 4,661
            Genes (coding)                    :: 3,205
            CDSs (with protein)               :: 3,205
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 1,456
            CDSs (without protein)            :: 1,456
            Pseudo Genes (ambiguous residues) :: 0 of 1,456
            Pseudo Genes (frameshifted)       :: 1,379 of 1,456
            Pseudo Genes (incomplete)         :: 85 of 1,456
            Pseudo Genes (internal stop)      :: 30 of 1,456
            Pseudo Genes (multiple problems)  :: 32 of 1,456
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Salmonella enterica subsp. enterica serovar
                     Ohio"
                     /mol_type="genomic DNA"
                     /strain="FSIS1606132"
                     /serovar="Ohio"
                     /isolation_source="animal-swine-market swine"
                     /sub_species="enterica"
                     /db_xref="taxon:117541"
                     /plasmid="pF18S005-2"
                     /geo_loc_name="USA:UT"
                     /collection_date="2016"
                     /collected_by="USDA-FSIS"
     gene            complement(23..457)
                     /locus_tag="A4H74_022840"
                     /pseudo=""
     CDS             complement(23..457)
                     /locus_tag="A4H74_022840"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(396..719)
                     /locus_tag="A4H74_022845"
     CDS             complement(396..719)
                     /locus_tag="A4H74_022845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UAD81778.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2009..2284)
                     /locus_tag="A4H74_022850"
     CDS             complement(2009..2284)
                     /locus_tag="A4H74_022850"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAD81777.1"
                     /translation="MVIVSVVGGISLLLLVFLWSIKRGQKTVRAFVFLSAVADGNSVES
                     ANELAKRIDLFAASELQKKAMIMVEMVFGGSQLKLISHARREGFDQ"
                     /name="hypothetical protein"
     gene            2682..3233
                     /locus_tag="A4H74_022855"
     CDS             2682..3233
                     /locus_tag="A4H74_022855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAD81774.1"
                     /translation="MKDMKAVVVFTGKDLNIMRTEGGSGYWHARTDRLNDADYLIAVRN
                     RRETWAVKDLEHGTAFLIAKITGCFKSPDYDDRNVITFDEYAEIHTPKAWKMLTDGQRY
                     PVAYLSAQEAFLRIGVTPEQLEWKKFHPSSPSVPNTVIPGLAEEKTEKLSLNEAIERAK
                     KDISNATGIDSSAITISIKI"
                     /name="hypothetical protein"
     gene            complement(3432..3647)
                     /locus_tag="A4H74_022860"
     CDS             complement(3432..3647)
                     /locus_tag="A4H74_022860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UAD81775.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3651..4136)
                     /locus_tag="A4H74_022865"
     CDS             complement(3651..4136)
                     /locus_tag="A4H74_022865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UAD81776.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSTLLTASGASILWWQGQQIL
                     DNYTIIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     Typing          complement(821..1006)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.421"
                     /coverage="87.0"
                     /evalue="9.61e-57"
     Typing          982..1469
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.081"
                     /coverage="94.0"
                     /evalue="7.71e-161"
     Typing          complement(3..406)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.778"
                     /coverage="99.0"
                     /evalue="8.77e-81"
     Typing          complement(3329..4416)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.46"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gccaggcatc aatgcgcgga cggtcggcgc ggtcgtaata cggctggaga cgcttgccgg
       61 tcagcagctc cgtgttcggg atcaggaagt tcagctccag ccgcccctta tcctggtgct
      121 caacccacag cacgctgtac tggtctttat cgagtccggg catcagaacc cgttcaaagc
      181 tcgccatcag cttttcgcgc tgcccgggcg gtaaatcctt ttcggcaaag gacaggacgc
      241 cggatgtgta cttttttgcg tagggcgagg cgtcgataag ctccctgact tcttccggct
      301 tcccctgcag aacggtggcg ccgtcgcgct gtcggtcttt ccccagcaga taatccaccg
      361 gaccggcgcc accgccgcgc cctcgcgggt gaaacttaac gatcatcgtc tgcacccttt
      421 tccagcacgg catgccgcag ccgctcgagt ccggcatcta tggccatcag cgcggcgacg
      481 acctgcacgc ggtcgtgccc gctgccacca ccggcgttaa cctgccgggc aatctggttg
      541 aggttgttcc ccatgccggc aagctgacga agcagcgccg gcgagagcga gggaagcttg
      601 ccggcgcggg cgggtttctc gtccaggcac gtctggcgca tccaggctgc aagctgttta
      661 ccgtcgcagc gctccagcag acggcggtgc tcatcttcgg tcacccacat cgtgagcatt
      721 ttgctgcgtt tgtctgccaa tccggcctcc tgataacgcc gggacgggcg ggaaatcttt
      781 tcccggtcgg cgcggtgttg gacaagccgc aggcgcgtca gtggttttag cgggtttcgg
      841 ggcgcagccc tgaaccagtc acgtagcgct agcggagtgt atactggctg aatatgttag
      901 atcggagtgg taattcaggg aagttattca tgttgcaagg gaaaatgcgg ctatagggcg
      961 taagtgcaaa atgcaggtaa cggtgacatg acgcctcctc gctcactcgg tcgctacgct
     1021 cctgccgtga ggctgcggcg ggcgttacct gctcacgaat gacaagataa agagatggca
     1081 ctcaagtcag gaaaggccgg aaaaaggata cggcgctgcc gtttttccat aggctccgcc
     1141 cccctgacga gcatcacaaa atcagacgct caaatcagtg gtggcgaaac ccgatgcggc
     1201 ttagagatac caggcgtttc cccctgatgg ctccctcgtg ctcccccgca ttcctgccct
     1261 tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac
     1321 tcagttccgg gtaggcagtt cgctccagga tggactgtat gcacgaaccc cccgttcagt
     1381 ccgactgctg cgccttgtcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa
     1441 aagcgccact ggcagcagcc actggtaaca ggtttagagg aggagagtct tgaaatttcg
     1501 ggtgcgataa ggctacactg aaaggacagt tttggtgact gtgctcctcc aagccagtta
     1561 ccgcggttca aaagagcgtc aactcttgcg aaccttcgaa aaaccacccg gcaaggtggt
     1621 tttttcgttt tcaaggcaag agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1681 tagattagtg ttcagagttg gtttcttcgg ttgtcatccg cctcccttta tctagccgat
     1741 gccatatcat aagggaaaag tcttatgtat aaatctatct ccccctatca attaggggct
     1801 actatagaac taataagggc ggactataga aggatagaat ccttatccgc catagaatag
     1861 aaaaaggagg ttaaatgcta aatgagttta tctatcttct acagatactg tctaaaaacc
     1921 cgccgaaaaa gaatatatat ttcgcagtag cgacctggtc acttagtctc tttatcatag
     1981 ttgtccaaca cgttggacaa tctacatttc actgatcgaa accttcccgt ctggcatgtg
     2041 agatcagttt aagctgactt cccccaaata ccatttcaac cattatcata gcctttttct
     2101 gcagttcact cgcagcaaaa agatctatcc gctttgccaa ctcgttagct gattcaacac
     2161 tatttccatc agcaacggca ctcagaaaaa caaatgctct cactgttttc tgacctcgtt
     2221 ttatcgacca taaaaacaca agaagcaaaa gtgatattcc acccacgaca ctaacaatca
     2281 ccatatttcg atcaccttat cttagataac ttcagaagtt gcttcattta ccataacatc
     2341 ccttgcccgc accgctccgg acgggttcaa cgtgaaagcg gcgttagccc ggtaaaaccg
     2401 gcagtaaact ccccgctaaa ctgcgcattc tgcgcctgat aaatttcacg cggctgcata
     2461 gccatgcagc cacgtgaatg gctcatgctt ttaatgtata tcctcgtcca agcccctgtt
     2521 ttaaccggtt tatcaaaaaa accaaacagg aagtcttacc actaacaata aaagaggtag
     2581 gggagactgt attttatcgc ataaaaaaac atttacaaca ccaattctat cgtatagatt
     2641 tcataaaaaa tgaaatttac tcacctatta agggtttcta tatgaaagat atgaaagctg
     2701 ttgtggtttt taccgggaag gatttgaaca taatgagaac tgagggggga tcaggctact
     2761 ggcatgccag aacagacagg ctcaacgatg ccgattacct catagcagta agaaacagaa
     2821 gggaaacgtg ggcagtaaaa gacctggaac acggcacagc attcctgatc gccaaaatta
     2881 ccgggtgttt taaaagccct gattacgacg acaggaacgt cataaccttt gatgaatacg
     2941 cagaaattca cacgccaaaa gcatggaaaa tgctaaccga tggtcagcgc tatccggttg
     3001 cttacctgag cgcacaggaa gcttttttac gtattggggt aacaccggaa caactggaat
     3061 ggaagaaatt ccatccttca tccccatcgg ttcctaatac agtgattcct ggtctggctg
     3121 aagagaaaac agaaaaactc tcccttaacg aggcaattga aagagccaaa aaggatatta
     3181 gcaacgcaac agggattgat agctcagcca taacaataag catcaagatc tgatgaagcg
     3241 tgaacaataa gatagcagcc tcatgttcta gaactgaacc tgtgcgccag aaacagcaag
     3301 cccatgcttt tccggcgcac agattcaaag tgacggaccg ttgtacgttc gctgtcgctg
     3361 agatcgttcc agctccagcg ctttctgatg ctgcgccgcc cgttcctgct cacgatgtgc
     3421 ctctatcacg ttcagccttg tgacaaccgg cgcagctgct cgctcaaact ctgcacctgc
     3481 ctgctcaagc ccgtgacgcg ctcgctcagc gccgcgttct cccgttgcat aagaccagac
     3541 atcgtccgcc attccgcgaa ggcgttctcc cactcgtcca gccttttcga gtagtcctgc
     3601 tgtagctgct ctaatgcgct cagcaactgt ttttccagct ccgtcatgtg ctatttcccc
     3661 gccagtatca tccagctcga atcctctccg aaccgtcccg cttccgggtt caccctcacg
     3721 cagcggcgtc tctgctctcc gcaggtcgag agctgcacgc cgctgttcct ctcggacagc
     3781 atggcctgcg tgctcttctg ctcccggatg atcgtgtaat tgtcgagtat ctgctgcccc
     3841 tgccaccaca gaatgcttgc gcccgatgct gtcagcagtg tagacaccag gacgatggtc
     3901 agccacgtct ggctgaccat acgcaccatg cctttcgtgc gctggctcat ggctgaggac
     3961 agcttccggt cgtggtcgag gatggcggcg ctgattctct tctcgctttc gctcagttcc
     4021 gctctgacag acttctcgtg ctcgctgaat gcggctttca gcatctcgcc ggtactctgc
     4081 tgctgcgctt tcgatttctg ctctaagtcc tttgccagcg ttaaaagact gttcatagat
     4141 ggctcccttc agtcggatgt ttcgcccccc gtccgggtcg gcaatgctga tgctgctttt
     4201 tgtggtgcgt accacctcaa aacctgcgct ttccagcgcc tcagtgacgt cctggcgggt
     4261 tttcagctcc ccggacgagg ctaaggccag taaaccccgt gtaattgcct gagcggcttc
     4321 ctgcttcgtt ttcggcagcg cggacggcgt gaccagcgcc cgccggtttt caggcgcgtt
     4381 cgggtcatgc agccccagcc tgccgttcac gatggtct
//
