LOCUS       CP082508               10910 bp    DNA     circular BCT 12-JAN-2022
DEFINITION  Salmonella enterica subsp. enterica serovar Muenchen strain
            FSIS1606504 plasmid pF18S006-2, complete sequence.
ACCESSION   CP082508
VERSION     CP082508.1
DBLINK      BioProject: PRJNA292666
            BioSample: SAMN04964189
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Muenchen
  ORGANISM  Salmonella enterica subsp. enterica serovar Muenchen
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 10910)
  AUTHORS   Li,C., Tyson,G.H., Hsu,C.H., Harrison,L., Strain,E., Tran,T.T.,
            Tillman,G.E., Dessai,U., McDermott,P.F. and Zhao,S.
  TITLE     Long-Read Sequencing Reveals Evolution and Acquisition of
            Antimicrobial Resistance and Virulence Genes in Salmonella enterica
  JOURNAL   Front Microbiol 12, 777817 (2021)
   PUBMED   34867920
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10910)
  CONSRTM   NARMS: The National Antimicrobial Resistance Monitoring System
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2018) Center for Veterinary Medicine, US Food and
            Drug Administration, 8401 Muirkirk Rd, Laurel, MD, USA
REFERENCE   3  (bases 1 to 10910)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2020
            Assembly Method :: SMRTLink Microbial Assembly v. SMRTLink9.0
            Assembly Name                     :: F18S006
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 343x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/02/2021 13:43:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,870
            CDSs (total)                      :: 4,747
            Genes (coding)                    :: 4,638
            CDSs (with protein)               :: 4,638
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 54 of 109
            Pseudo Genes (incomplete)         :: 63 of 109
            Pseudo Genes (internal stop)      :: 15 of 109
            Pseudo Genes (multiple problems)  :: 22 of 109
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10910
                     /organism="Salmonella enterica subsp. enterica serovar
                     Muenchen"
                     /mol_type="genomic DNA"
                     /strain="FSIS1606504"
                     /serovar="Muenchen"
                     /isolation_source="animal-cattle-dairy cow"
                     /sub_species="enterica"
                     /db_xref="taxon:596"
                     /plasmid="pF18S006-2"
                     /geo_loc_name="USA:TN"
                     /collection_date="2016"
                     /collected_by="USDA-FSIS"
     gene            complement(194..1045)
                     /locus_tag="A7Q27_024240"
     CDS             complement(194..1045)
                     /locus_tag="A7Q27_024240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="UAE12655.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="replication protein"
     gene            complement(1032..1871)
                     /locus_tag="A7Q27_024245"
     CDS             complement(1032..1871)
                     /locus_tag="A7Q27_024245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="UAE12656.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            complement(1901..2107)
                     /locus_tag="A7Q27_024250"
     CDS             complement(1901..2107)
                     /locus_tag="A7Q27_024250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAE12657.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="hypothetical protein"
     gene            complement(2109..2321)
                     /locus_tag="A7Q27_024255"
     CDS             complement(2109..2321)
                     /locus_tag="A7Q27_024255"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAE12658.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            complement(2382..4511)
                     /locus_tag="A7Q27_024260"
     CDS             complement(2382..4511)
                     /locus_tag="A7Q27_024260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UAE12659.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHG
                     LVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEY
                     DADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQI
                     EQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQK
                     LASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPA
                     PRQRGMDRGGPDFSM"
                     /name="MobA/MobL family protein"
     gene            4710..4994
                     /locus_tag="A7Q27_024265"
     CDS             4710..4994
                     /locus_tag="A7Q27_024265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="UAE12660.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            5592..5780
                     /locus_tag="A7Q27_024270"
                     /pseudo=""
     CDS             5592..5780
                     /locus_tag="A7Q27_024270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(5747..6946)
                     /gene="tet(A)"
                     /locus_tag="A7Q27_024275"
     ARG             complement(5751..6946)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7052..7702
                     /gene="tetR(A)"
                     /locus_tag="A7Q27_024280"
     CDS             7052..7702
                     /gene="tetR(A)"
                     /locus_tag="A7Q27_024280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="UAE12661.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(7734..7976)
                     /locus_tag="A7Q27_024285"
     CDS             complement(7734..7976)
                     /locus_tag="A7Q27_024285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="UAE12651.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="relaxase"
     gene            complement(8282..9118)
                     /locus_tag="A7Q27_024290"
     CDS             complement(8282..9118)
                     /locus_tag="A7Q27_024290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="UAE12652.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     ARG             complement(9119..9537)
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             complement(9122..9921)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(9982..10797)
                     /gene="sul2"
                     /locus_tag="A7Q27_024300"
     ARG             complement(9986..10797)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(8286..9118)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(4601..4756)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          complement(1033..1854)
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(2386..4511)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagagcatac atctggaagc aaagccagga aagcggccta tggagctgtg cggcagcgct
       61 cagtaggcaa tttttcaaaa tattgttaag ccttttctga gcatggtatt tttcatggta
      121 ttaccaatta gcaggaaaat aagccattga atataaaaga taaaaatgtc ttgtttacaa
      181 tagagtgggg gggtcagcct gccgccttgg gccgggtgat gtcgtacttg cccgccgcga
      241 actcggttac cgtccagccc agcgcgacca gctccggcaa cgcctcgcgc acccgctggc
      301 ggcgcttgcg catggtcgaa ccactggcct ctgacggcca gacatagccg cacaaggtat
      361 ctatggaagc cttgccggtt ttgccggggt cgatccagcc acacagccgc tggtgcagca
      421 ggcgggcggt ttcgctgtcc agcgcccgca cctcgtccat gctgatgcgc acatgctggc
      481 cgccacccat gacggcctgc gcgatcaagg ggttcagggc cacgtacagg cgcccgtccg
      541 cctcgtcgct ggcgtactcc gacagcagcc gaaacccctg ccgcttgcgg ccattctggg
      601 cgatgatgga taccttccaa aggcgctcga tgcagtcctg tatgtgcttg agcgccccac
      661 cactatcgac ctctgccccg atttcctttg ccagcgcccg atagctacct ttgaccacca
      721 tggcatcagc ggtgacggcc tcccacttgg gttccaggaa cagccggagc tgccgtccgc
      781 cttcggtctt gggttccggg ccaagcacta ggccattagg cccagccatg gccaccagcc
      841 cttgcaggat gcgcagatca tcagcgccca gcggctccgg gccgctgaac tcgatccgct
      901 tgccgtcgcc gtagtcatac gtcacgtcca gcttgctgcg cttgcgctcg ccccgcttga
      961 gggcacggaa caggccgggg gccagacagt gcgccgggtc gtgccggacg tggctgaggc
     1021 tgtgcttgtt cttaggcttc accacggggc acccccttgc tcttgcgctg cctctccagc
     1081 acggcgggct tgagcacccc gccgtcatgc cgcctgaacc accgatcagc gaacggtgcg
     1141 ccatagttgg ccttgctcac accgaagcgg acgaagaacc ggcgctggtc gtcgtccaca
     1201 ccccattcct cggcctcggc gctggtcatg ctcgacaggt aggactgcca gcggatgtta
     1261 tcgaccagta ccgagctgcc ccggctggcc tgctgctggt cgcctgcgcc catcatggcc
     1321 gcgcccttgc tggcatggtg caggaacacg atagagcacc cggtatcggc ggcgatggcc
     1381 tccatgcgac cgatgacctg ggccatgggg ccgctggcgt tttcttcctc gatgtggaac
     1441 cggcgcagcg tgtccagcac catcaggcgg cggccctcgg cggcgcgctt gaggccgtcg
     1501 aaccactccg gggccatgat gttgggcagg ctgccgatca gcggctggat cagcaggccg
     1561 tcagccacgg cttgccgttc ctcggcgctg aggtgcgccc caagggcgtg caggcggtga
     1621 tgaatggcgg tgggcgggtc ttcggcgggc aggtagatca ccgggccggt gggcagttcg
     1681 cccacctcca gcagatccgg cccgcctgca atctgtgcgg ccagttgcag ggccagcatg
     1741 gatttaccgg caccaccggg cgacaccagc gccccgaccg taccggccac catgttgggc
     1801 aaaacgtagt ccagcggtgg cggcgctgct gcgaacgcct ccagaatatt gataggctta
     1861 tgggtagcca ttgattgcct cctttgcagg cagttggtgg ttaggcgctg gcggggtcac
     1921 tacccccgcc ctgcgccgct ctgagttctt ccaggcactc gcgcagcgcc tcgtattcgt
     1981 cgtcggtcag ccagaacttg cgctgacgca tccctttggc cttcatgcgc tcggcatatc
     2041 gcgcttggcg tacagcgtca gggctggcca gcaggtcgcc ggtctgcttg tccttttggt
     2101 ctttcatatc agtcaccgag aaacttgccg gggccgaaag gcttgtcttc gcggaacaag
     2161 gacaaggtgc agccgtcaag gttaaggctg gccatatcag cgactgaaaa gcggccagcc
     2221 tcggccttgt ttgacgtata accaaagcca ccgggcaacc aatagccctt gtcacttttg
     2281 atcaggtaga ccgaccctga agcgcttttt tcgtattcca taaaaccccc ttctgtgcgt
     2341 gagtactcat agtataacag gcgtgagtac caacgcaagc actacatgct gaaatctggc
     2401 ccgcccctgt ccatgcctcg ctggcggggt gccggtgccc gtgccagctc ggcccgcgca
     2461 agctggacgc tgggcagacc catgaccttg ctgacggtgc gctcgatgta atccgcttcg
     2521 tggccgggct tgcgctctgc cagcgctggg ctggcctcgg ccatggcctt gccgatttcc
     2581 tcggcactgc ggccccggct ggccagcttc tgcgcggcga taaagtcgca cttgctgagg
     2641 tcatcaccga agcgcttgac cagcccggcc atctcgctgc ggtactcgtc cagcgccgtg
     2701 cgccggtggc ggctaagctg ccgctcgggc agttcgaggc tggccagcct gcgggccttc
     2761 tcctgctgcc gctgggcctg ctcgatctgc tggccagcct gctgcaccag cgccgggcca
     2821 gcggtggcgg tcttgccctt ggattcacgc agcagcaccc acggctgata accggcgcgg
     2881 gtggtgtgct tgtccttgcg gttggtgaag cccgccaagc ggccatagtg gcggctgtcg
     2941 gcgctggccg ggtcggcgtc gtactcgctg gccagcgtcc gggcaatctg cccccgaagt
     3001 tcaccgcctg cggcgtcggc caccttgacc catgcctgat agttcttcgg gctggtttcc
     3061 actaccaggg caggctcccg gccctcggct ttcatgtcat ccaggtcaaa ctcgctgagg
     3121 tcgtccacca gcaccagacc atgccgctcc tgctcggcgg gcctgatata cacgtcattg
     3181 ccctgggcat tcatccgctt gagccatggc gtgttctgga gcacttcggc ggctgaccat
     3241 tcccggttca tcatctggcc ggtggtggcg tccctgacgc cgatatcgaa gcgctcacag
     3301 cccatggcct tgagctgtcg gcctatggcc tgcaaagtcc tgtcgttctt catcgggcca
     3361 ccaagcgcag ccagatcgag ccgtcctcgg ttgtcagtgg cgtcaggtcg agcaagagca
     3421 acgatgcgat cagcagcacc accgtaggca tcatggaagc cagcatcacg gttagccata
     3481 gcttccagtg ccacccccgc gacgcgctcc gggcgctctg cgcggcgctg ctcacctcgg
     3541 cggctacctc ccgcaactct ttggccagct ccacccatgc cgcccctgtc tggcgctggg
     3601 ctttcagcca ctccgccgcc tgcgcctcgc tggcctgctg ggtctggctc atgacctgcc
     3661 gggcttcgtc ggccagtgtc gccatgctct gggccagcgg ttcgatctgc tccgctaact
     3721 cgttgatgcc tctggatttc ttcactctgt cgattgcgtt catggtctat tgcctcccgg
     3781 tattcctgta agtcgatgat ctgggcgttg gcggtgtcga tgttcagggc cacgtctgcc
     3841 cggtcggtgc ggatgccccg gccttccatc tccaccacgt tcggccccag gtgaacaccg
     3901 ggcaggcgct cgatgccctg cgcctcaagt gttctgtggt caatgcgggc gtcgtggcca
     3961 gcccgctcta atgcccggtt ggcatggtcg gcccatgcct cgcgggtctg ctcaagccat
     4021 gccttgggct tgagcgcttc ggtcttctgt gccccgccct tctccggggt cttgccgttg
     4081 taccgcttga accactgagc ggcgggccgc tcgatgccgt cattgatccg ctcggagatc
     4141 atcaggtggc agtgcgggtt ctcgccgcca ccggcatgga tggccagcgt atacggcagg
     4201 cgctcggcac cggtcaggtg ctgggcgaac tcggacgcca gcgccttctg ctggtcgagg
     4261 gtcagctcga ccggcagggc aaattcgacc tccttgaaca gccgcccatt ggcgcgttca
     4321 tacaggtcgg cagcatccca gtagtcggcg ggccgctcga cgaactccgg catgtgcccg
     4381 gattcggcgt gcaagacttc atccatgtcg cgggcatact tgccttcgcg ctggatgtag
     4441 tcggccttgg ccctggccga ttggccgccc gacctgctgc cggttttcgc cgtaaggtga
     4501 taaatcgcca tgctgcctcg ctgttgcttt tgcttttcgg ctccatgcaa tggccctcgg
     4561 agagcgcacc gcccgaaggg tggccgttag gccagtttct cgaagagaaa ccggtaagtg
     4621 cgccctcccc tacaaagtag ggtcgggatt gccgccgctg tgcctccatg atagcctacg
     4681 agacagcaca ttaacaatgg ggtgtcaaga tggttaaggg gagcaacaag gcggcggatc
     4741 ggctggccaa gctcgaagaa caacgagcgc gaatcaatgc cgaaattcag cgggtgcggg
     4801 caagggaaca gcagcaagag cgcaagaacg aaacaaggcg caaggtgctg gtgggggcca
     4861 tgattttggc caaggtgaac agcagcgagt ggccggagga tcggctcatg gcggcaatgg
     4921 atgcgtacct tgaacgcgac cacgaccgcg ccttgttcgg tctgccgcca cgccagaagg
     4981 atgagccggg ctgaatgatc gaccgagaca ggccctgcgg ggctgcacac gcgcccccac
     5041 ccttcgggta gggggaaagg ccgctaaagc ggctaaaagc gctccagcgt atttctgcgg
     5101 ggtttggtgt ggggtttagc gggctttgcc cgcctttccc cctgccgcgc agcggtgggg
     5161 cggtgtgtag cctagcgcag cgaatagacc agctatccgg cctctggccg ggcatattgg
     5221 gcaagggcag cagcgcccca caagggcgct gataaccgcg cctagtggat tattcttaga
     5281 taatcatgga tggatttttc caacaccccg ccagcccccg cccctgctgg gtttgcaggt
     5341 ttgggggcgt gacagttatt gcaggggttc gtgacagtta ttgcaggggg gcgtgacagt
     5401 tattgcaggg gggcgtgaca gttattgcag gggggcgtga cagttattgc aggggggcgt
     5461 gacagttagt acgggagtga cgggcactgg ctggcaatgt ctagcaacgg caggcatttc
     5521 ggctgagggt aaaagaactt tccgctaagc gatagactgt atgtaaacac agtattgcaa
     5581 ggacgcggaa catgcctcat gtggcggcca ggacggccag ccgggatcgg gatactggtc
     5641 gttaccagag ccaccgaccc gagcaaaccc ttctctatca gatcgttgac gagtattacc
     5701 cggcattcgc tgcgcttatg gcagagcagg gaaaggaatt gccgggctat gtgcaacggg
     5761 aatttgaaga atttctccaa agcccgcgac gcagcgccgg caggcagagc aagtagaggg
     5821 cagcgcctgc aatccatgcc cacccgttcc acgttgttat agaagccgca tagatcgccg
     5881 tgaagaggag gggtccgacg atcgaggtca ggctggtgag cgccgccagt gagccttgca
     5941 gctgcccctg acgttcctca tccacctgcc tggacaacat tgcttgcagc gccggcattc
     6001 cgatgccacc cgaagcaagc aggaccatga tcgggaacgc catccatccc cgtgtcgcga
     6061 aggcaagcag gatgtagcct gtgccgtcgg caatcattcc gagcatgagt gcccgccttt
     6121 cgccgagccg ggcggctaca gggccggtga tcattgcctg ggcgagtgaa tgcagaatgc
     6181 caaatgcggc aagcgaaatg ccgatcgtgg tcgcgtccca gtgaaagcga tcctcgccga
     6241 aaatgaccca aagcgcggcc ggcacctgtc cgacaagttg catgatgaag aagaccgcca
     6301 tcagggcggc gacgacggtc atgccccggg cccaccggaa cgaagcgagc gggttgagag
     6361 cctcccggcg taacggccgg cgttcgcctt tgtgcgactc cggcaaaagg aaacagcccg
     6421 tcaggaaatt gaggccgttc aaggctgccg cggcgaagaa cggagcgtgg ggggagaaac
     6481 cgcccatcag cccaccgagc acaggtcccg cgaccatccc gaacccgaaa caggcgctca
     6541 tgaagccgaa gtgccgcgcg cgctcatcgc catcagtgat atcggcaata taagcgccgg
     6601 ctaccgcccc agtcgccccg gtgatgccgg ccacgatccg cccgatatag agaacccaaa
     6661 ggaaaggcgc cgtcgccatg atggcgtagt cgacagcagc gccggccagc gagacgagca
     6721 agaccggccg ccgcccgaaa cgatccgaca gcgcgcccag cacaggtgcg caggcaaatt
     6781 gcatcaacgc atacagcgcc agcagaatgc catagtgggc ggtgacgtcg ttcgagtgaa
     6841 ccagatcgcg caggaggccc ggcagcaccg gcataatcag gccgatgccg acagcgtcga
     6901 gcgcgacagt gctcagaatt acgatcaggg gtctgttggg tttcacgtct ggcctccgga
     6961 ccagcctccg ctggtccgat tgaacgcgcg gattctttat cactgataag ttggtggaca
     7021 tattatgttt atcagtgata aagtgtcaag catgacaaag ttgcagccga atacagtgat
     7081 ccgtgccgcc ctggacctgt tgaacgaggt cggcgtagac ggtctgacga cacgcaaact
     7141 ggcggaacgg ttgggggttc agcagccggc gctttactgg cacttcagga acaagcgggc
     7201 gctgctcgac gcactggccg aagccatgct ggcggagaat catacgcatt cggtgccgag
     7261 agccgacgac gactggcgct catttctgat cgggaatgcc cgcagcttca ggcaggcgct
     7321 gctcgcctac cgcgatggcg cgcgcatcca tgccggcacg cgaccgggcg caccgcagat
     7381 ggaaacggcc gacgcgcagc ttcgcttcct ctgcgaggcg ggtttttcgg ccggggacgc
     7441 cgtcaatgcg ctgatgacaa tcagctactt cactgttggg gccgtgcttg aggagcaggc
     7501 cggcgacagc gatgccggcg agcgcggcgg caccgttgaa caggctccgc tctcgccgct
     7561 gttgcgggcc gcgatagacg ccttcgacga agccggtccg gacgcagcgt tcgagcaggg
     7621 actcgcggtg attgtcgatg gattggcgaa aaggaggctc gttgtcagga acgttgaagg
     7681 accgagaaag ggtgacgatt gatcaggacc gctgccggag cgcaacccac tcactacagc
     7741 agagccatgt agacaacatc ccctccccct ttccaccgcg tcagacgccc gtagcagccc
     7801 gctacgggct ttttcatgcc ctgccctagc gtccaagcct cacggccgcg ctcggcctct
     7861 ctggcggcct tctggcgctc ctgctgcggc gtccgctcgt gggccgtggc gcgggtccgc
     7921 gcgccggcct cgtgcgcctg gcgctcgcgg gcgaggtcca gggcggccgt cttcacgttc
     7981 tgccttgcgc agatgagata gatctagcgt ggactcaagg ctctcgcgaa tggctcgcgt
     8041 tggaaacttt cattgacact tgaggggcac cgcagggaaa ttctcgtcct tgcgagaacc
     8101 ggctatgtcg tgctgcgcat cgagcctgcg cccttggctt gtctcgcccc tctccgcgtc
     8161 gctacggggc ttccaggggt cgtttgcggg aaggggcgga atcctacgct aaggctttgg
     8221 ccagcgatat tctccggtga gattgatgtg ttcccagggg ataggagaag tcgcttgata
     8281 tctagtatga cgtctgtcgc acctgcttga tcgcggccgc gatagctaga tcgcgttgct
     8341 cctcttctcc atccgcgttc caagctgcgg aaaggcatcc ataagcgtac gcctggtcga
     8401 gcaggcgacg cggatcgacg tccagcgcac gagagaatgc gtccgccatc tgtgcaatgc
     8461 gtctaggatc gagacaaagg tcgtctctgt cagccggatc gtagaacata ttggcggcgc
     8521 caaagcccac ttcaccgacc agaccgacgg gatctatcac cagccagccg cgactggaga
     8581 acatgatgtt ttcatgatgc agatcgccat gtagcccacg cagttccgag gcattgctca
     8641 tcatttgatc ggctataatc gccgcgtgga cgtagtcagt ttgacaacct gcgttttgat
     8701 catcgcgcgc ccgctgaaac aaagctgcaa agcgatcccg gatcgggaga agggcagaag
     8761 gcaggggttc ctcagatgcg gcatacagct tcgccattag ttccgctgca atttcggtcg
     8821 cctggtagtc gccgtgctcg gcaacgatgt gagagagcat tcgctccccg gcatattcga
     8881 gcaacatcag attgttctca cgaccgagca accggactgc tcccctccca ttgcgccata
     8941 ccagatagtc ggccccgcgc agttcatcag caatgtcttc tataggtttc aatcccttga
     9001 cgattgcagg agtcccgtct ggcaatgaaa ctttccaaac gaggctggaa aaggtgtccg
     9061 caatgagaac aggttgcgaa acgtgccaat gagcaggaaa aacaggcggc atgaacatca
     9121 accccaagtc agagggtcca atcgcagata gaaggcaagg cgttcgcggt cgggggcttc
     9181 gatccccaat acattgaata ggacagcgaa ggcgcgctct gcttcatctg gcgctgccca
     9241 gttctcttcg gcgttagcaa tcatgagtgc caaatcggca tagcgatctg ctgttccgag
     9301 ccgcccaagg tcgatcagac ccgtgcattg aagagtttta gggtccacca tgaagttcgg
     9361 catgcaggga tcaccatggc aaacaaccat atcggtgcgc tcttggtcga gccgcaccgg
     9421 tagctctcgt tcgacacgag ccaaaagatc gagctgcggc gtactcttgt cctcgtccgg
     9481 taagaagtcg ggattgacgg cattgcggga caccacatca acggcgcgtc cgaacattcg
     9541 cgacagcctg cgctcaaacg gacattgatc aaccgatagg ctgtgaacag cgccaagttg
     9601 ctgccccatt gacggccacg ctttgagcaa atccgctcca gacagatcag ccgccggtac
     9661 tcccggaatt gccgttatca ccaagcatgc accctcctgt tcctcctgcc agttgatgac
     9721 ctcggggcaa gccacacctc gacctttgag ccaaatgagg cggtcacgct ctccagcgag
     9781 ctcaccgcgg cgggaagcag gtgcgatttt cgcgaaggca tgcccgtcac cacgtcgaaa
     9841 aacaaaatca ccagattctc cgcctctgac aggcaaccag tcagaatgcg attcaccaaa
     9901 aaaaatatta gttcgattca atggaggttc cttcagtttt ctgatgaagc gcgaatatag
     9961 agaaatatcc cgaatgtgca gttaacgaat tcttgcggtt tctttcagcg ccgccaatac
    10021 cgccagcccg tcgcgcaagg ggcgcggctc gtgtgtgcgg atgaagtcag ctccacctgc
    10081 ggcggcggca agctctgcag cgagtgtcgc ggccccgaca tcccccggac cacggcctgt
    10141 gagcgcgcgc agaaaggatt tgcgcgaaac agacagaagc accggcaaat cgaagcgcag
    10201 ccgcaattca tcgaaccgcg ccagcaccga gagcgaggtt tcgggagcag cccccagaaa
    10261 aaaccccatg ccgggatcaa ggacaaggcg gttgcgtttg ataccggcac ccgtcagcgc
    10321 cgcgatgcgc gcgtcaaaga acgccgcaat gtgatccatg atgtcgccag cgggtgcctc
    10381 gcgccgatct gcctgcccgt cttgcaccga atgcataacg acgagtttgg cagatgattt
    10441 cgccaattgc ggatagaacg cagcgtctgg aaaaccgcga atatcattga gataggccac
    10501 accacgcgac aaggcatagg cttgcgtcgc gggttgataa ctgtcgagcg agacgggaat
    10561 gccatctgcc ttgagcgcgt ccagcaccgg cgcgatacgc gcgatttctg tgtcggacga
    10621 aacaggcgcg gcgtcgggat tgctggatgc cggaccgagg tcgatcacat ctgccccctc
    10681 ggccatcagc ttacgcgcct gcgcaatggc tgcgtctggc gccagatacc ggcctccatc
    10741 ggagaaactg tccgaggtta tgttgacgat gccgaaaatg atgagcgatt tattcatggg
    10801 ggcttctata ataataataa tcgagcatga gtctcatacg gatgctcggg tcgaaaggga
    10861 atccccaggc gagtaacctg tttgcggtga tccattagct gcaggagcag
//
