LOCUS       CP082388               10511 bp    DNA     circular BCT 11-JAN-2022
DEFINITION  Salmonella enterica subsp. enterica serovar Reading strain
            FSIS1701482 plasmid pF18S042-2, complete sequence.
ACCESSION   CP082388
VERSION     CP082388.1
DBLINK      BioProject: PRJNA292666
            BioSample: SAMN07237761
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Reading
  ORGANISM  Salmonella enterica subsp. enterica serovar Reading
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 10511)
  AUTHORS   Li,C., Tyson,G.H., Hsu,C.H., Harrison,L., Strain,E., Tran,T.T.,
            Tillman,G.E., Dessai,U., McDermott,P.F. and Zhao,S.
  TITLE     Long-Read Sequencing Reveals Evolution and Acquisition of
            Antimicrobial Resistance and Virulence Genes in Salmonella enterica
  JOURNAL   Front Microbiol 12, 777817 (2021)
   PUBMED   34867920
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10511)
  CONSRTM   NARMS: The National Antimicrobial Resistance Monitoring System
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2018) Center for Veterinary Medicine, US Food and
            Drug Administration, 8401 Muirkirk Rd, Laurel, MD, USA
REFERENCE   3  (bases 1 to 10511)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2020
            Assembly Method :: SMRTLink Microbial Assembly v. SMRTLink9.0
            Assembly Name                     :: F18S042
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/02/2021 10:27:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,525
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,295
            CDSs (with protein)               :: 4,295
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 50 of 110
            Pseudo Genes (incomplete)         :: 60 of 110
            Pseudo Genes (internal stop)      :: 23 of 110
            Pseudo Genes (multiple problems)  :: 20 of 110
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10511
                     /organism="Salmonella enterica subsp. enterica serovar
                     Reading"
                     /mol_type="genomic DNA"
                     /strain="FSIS1701482"
                     /serovar="Reading"
                     /isolation_source="Animal-Turkey-Young Turkey"
                     /sub_species="enterica"
                     /db_xref="taxon:165302"
                     /plasmid="pF18S042-2"
                     /geo_loc_name="USA:AR"
                     /collection_date="2017"
                     /collected_by="USDA-FSIS"
     gene            452..772
                     /locus_tag="CED08_022575"
     CDS             452..772
                     /locus_tag="CED08_022575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UAF56466.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNTGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGND
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            735..1475
                     /locus_tag="CED08_022580"
                     /pseudo=""
     CDS             735..1475
                     /locus_tag="CED08_022580"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1450..1935
                     /locus_tag="CED08_022585"
     CDS             1450..1935
                     /locus_tag="CED08_022585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UAF56467.1"
                     /translation="MSSLLALAKDLEKQSKAQQQNTCEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEQKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            1939..2151
                     /locus_tag="CED08_022590"
     CDS             1939..2151
                     /locus_tag="CED08_022590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UAF56468.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2822..3682)
                     /locus_tag="CED08_022595"
     CDS             complement(2822..3682)
                     /locus_tag="CED08_022595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="UAF56469.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="class A broad-spectrum beta-lactamase TEM-1"
     gene            complement(3865..4422)
                     /locus_tag="CED08_022600"
     CDS             complement(3865..4422)
                     /locus_tag="CED08_022600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="UAF56470.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            4586..5356
                     /locus_tag="CED08_022605"
                     /pseudo=""
     CDS             4586..5356
                     /locus_tag="CED08_022605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     gene            5358..7718
                     /locus_tag="CED08_022610"
                     /pseudo=""
     CDS             5358..7718
                     /locus_tag="CED08_022610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /name="Tn3-like element Tn3 family transposase"
     gene            8054..8464
                     /locus_tag="CED08_022615"
     CDS             8054..8464
                     /locus_tag="CED08_022615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAF56464.1"
                     /translation="MAFISLSNLAAKTGRIIQSQCVTATQTKKSTGAVELGFRLSKSAM
                     DETGITIGDRVDILYDKEENQWMIKKISEGGFGVSGQKNKHGGYSSCAVRLTLRPGFPR
                     LSEDEEDNKKLLSDDSFTQLSEGVIVFRLDDE"
                     /name="hypothetical protein"
     gene            8722..9285
                     /locus_tag="CED08_022620"
     CDS             8722..9285
                     /locus_tag="CED08_022620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAF56465.1"
                     /translation="MHALSILPSVARANLATKFSSHLKVIELFNTMQDSQVVVLSSLIE
                     GHHLTSSGNSVKADFEVTRLPAIIEMLEKKYFFPIRHLNVSVKSVTTGRMTGQTVYFIE
                     PEHIEQLLADPELVFANQERSLFFRSLEKEGKNLGKLIEKKGSVSQAVLSLLHHAYKDK
                     PLSDEMWKEIEDKFTHMLDELSAA"
                     /name="hypothetical protein"
     gene            complement(9314..9775)
                     /locus_tag="CED08_022625"
     CDS             complement(9314..9775)
                     /locus_tag="CED08_022625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAF56471.1"
                     /translation="MSKQDYIQLRTLTDQFYADNKGLQEALDGSNDGKVRGYGIVVIDL
                     NGLVFGIPLRSHLNHKFGFVSERSEGVKKGLDYTKALLIKKEEYVSRAYKIPTPEFTHI
                     NDNKEKIQEDFNKFVNRYIEANVKKDENILRNYRYSTLKNYHKELGLED"
                     /name="hypothetical protein"
     ARG             complement(2826..3682)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          165..344
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.339"
                     /coverage="83.0"
                     /evalue="7.539999999999999e-48"
     Typing          complement(2..219)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.868"
                     /coverage="99.0"
                     /evalue="2.42e-76"
     Typing          complement(10042..10507)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.885"
                     /coverage="91.0"
                     /evalue="2.88e-145"
     Typing          763..2267
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.426"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggcagcg
       61 ccgcatcctt ttcttgccct gtttgtcatg ccgtatgcct ttgctcttat ccgtgtgcag
      121 gtaacgcccg ccgcagccga acggcaggag cggaagcgac cgagtgagcg aggaggcgta
      181 atatcaccgc aacctccatg ttctcccgta cgccctgcag ctgcattttc ccctgccaca
      241 tgaagcactt ttataaagtg ttactccgcc gtaacatatt aagccagtat acactccgct
      301 agcgctacgt gactggttca gggctgcgcc ccgaaacccg ctaaaagcca ctgacgcgcc
      361 tgcggcttgt ccaacaccgt gccgaccggg aaacatttcc cgcccgtccc ggtgttatca
      421 ggaggctact ctggcagaca aacgcaacaa gatgctcacg atgtgggtga cggaagacga
      481 acaccggcgg ctgctcgagc ggtgcgacgg caagcagctg gcggcgtgga tgcgtcagac
      541 ctgcctcgat gagaagccgg cgcgtgccgg cagactgccg tcgatatcgc cggcgctgct
      601 gcgccagctt gccggtatgg gtaataacct gaaccagatc gcccggaaag tgaataccgg
      661 tggtgccggt catgaccggg tgcagatcgt ggccgccctg atggcaatcg acgcgggact
      721 ggagcggctg cggcatgcgg tgctggaaaa aggggggaat gatgatcgtt aaattccatc
      781 cccgggggcg cggtggtggt gccggtccgg tggattacct gctgggaaaa gaccggcagc
      841 gcgagggggc gaccgtgctg cagggaaagc cggaggaagt ccgggagctt atcgatgcct
      901 cgccgtatgc gaaaaagtac acctccggcg tactgtcgtt cgcggaggct gagctgccgc
      961 ccgggcagcg ggagcagatc atggccagct ttgagcgggt tctgatgccc gggctcgata
     1021 aagaccagta cagcattctg tgggtggagc acaccgacaa ggggcggctg gagctgaatt
     1081 tcctgatccc caacacagag ctgctgaccg ggaaacgcct gcagccgtat tacgatcgcg
     1141 ccgaccggcc gcgcatcgat gcctggcaga ccgtggtgaa cggcagactg gggctgcatg
     1201 atccaaacgc cccgaagaac cggcggttac tggtcacgcc gtccgcactg ccggaaacga
     1261 agctggaagc cgctcaggcg attacacggg gtttgctggc cttagcctca tccggggcgc
     1321 tcaaaacgcg cgaggacgtc acagggtcgc tcactgcggc aggttttgag gtggtccgca
     1381 ccacaaagaa cagtatcagc attgccgacc cggacggggg gcgaaacatc cgactgaagg
     1441 gagccattta tgagcagtct tttagcgctg gcgaaggact tggaaaagca atcgaaagcg
     1501 cagcagcaga atacctgcga gatgctgaaa gccgcattca gcgagcacga gaagtctgta
     1561 aaagcggaac tgagcgcaag cgcgaagaga atcagcgacg ccatcagcgc ccacgagcag
     1621 ggtatgaccg cggcgatgca gtcgaaccgg ctgagcgtgc tgcgtatggt tggccggacg
     1681 tggctgacca tcaccctggt ctcagtactg ctgatcgcca cgagcgggag cattctgtgg
     1741 tggcaggggc agcagatcac cgggaattac cagactatcc gggcgcagga gcggacgcag
     1801 gccatgctgt cggagaagaa ccacggcgtg cagctgtcgc tctgtgggga acagaaactg
     1861 tcctgcgtca aagtgaaccc gaaagcggga gcttacgggg aggaagggaa ctggatggta
     1921 ctggagagga aatagcccat gacggaactg gaaaaacagt tgctgaccgc attagagcag
     1981 ctacatcagg actactcgca aaggctggac gagtgggaga gcgccttcgc ggaatggcag
     2041 aagatgtctg gtcttatgca acgggagaac gcggcgctga acgagcgcgt cacgcgcttg
     2101 agtcagcagg tggcgaactt gagcaggcag ttacagcgtt tgagccagta gtgcagcggc
     2161 atgaaatggc ggtggccgcc gaacgggcat acgagcagcg acagcatgaa aaagagctgg
     2221 cagaaccccg tttccgtcag cggagctata acggtccgac gctgggctga ctgcgcgcat
     2281 ccagtgacaa accccaagaa gaagggctca taaatttccc gctaaatacg gtcgtgatga
     2341 aaagatcacg gctcaagaga gggggattta tgaagatact aaaagcgctc agtctgatac
     2401 tgcaatgcgt caaactggca atggatctgt gggagcgatt cgggggaaag tggccttttt
     2461 agtgctttac accgtattta tacgcgaaaa ctgctgtcat tcatctggct gcataaccat
     2521 gcagccaagt gaaatttatc tggcgtaaac ggggcggttt accgggtgct ttgctagggg
     2581 tttcacccga caggcaccgt atttgcgccg aaccagcccg aagcggtgcg ggcaagaact
     2641 cttatgttaa attcatctca tcggcaacct atacggggtc tgacgctcag tggaacgaaa
     2701 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2761 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     2821 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2881 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2941 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3001 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3061 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3121 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3181 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3241 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3301 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     3361 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3421 gctcttgccc ggcgtcaaca cgggataata ccgcaccaca tagcagaact ttaaaagtgc
     3481 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3541 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3601 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3661 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3721 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     3781 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     3841 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttttataaac
     3901 cgtggagcgg gcaatactga gctgatgagc aatttccgtt gcaccagtgc ccttctgatg
     3961 aagcgtcagc acgacgttcc tgtccacggt acgcctgcgg ccaaatttga ttcctttcag
     4021 ctttgcttcc tgtcggccct cattcgtgcg ttctaggatc ctccggcgtt cagcctgtgc
     4081 cacagccgac aggatggtga ccaccatttg ccccatatca ccgtcggtac tgatcccgtc
     4141 atcaatgaac cggactgcca cgccctgagc gtcaaattcc tttatcagtt ggatcatatc
     4201 ggcagtgtcg cggccaagac ggtcgagctt cttaaccaga atgacatcac cttcctccac
     4261 cttcatcctc agcaaatcca gcccttcccg gtctgttgaa ctgccggatg ccttatcggt
     4321 aaatatacgg tttgctttca cacctgcgtc tttgagtgct ctgacctgaa gatcaagaga
     4381 ctgctgactg gttgagaccc gagcgtaacc aaaaagtcgc ataaaaatgt accttaaatc
     4441 gaatatcgga caactcatgt ctattattac aaatttacga tttaatagac atattaatgt
     4501 aacagtttta cgatgtccga taatttataa catttcgtac ggttgcaaaa atgttactaa
     4561 atgcccgtca ggcagggagg ccgatatgcc cgttgacttt ctgaccactg agcagactga
     4621 aagctatggc agattcaccg gtgaaccgga tgagcttcag ctggcacgat attttcacct
     4681 tgatgaagca gacaaggaat ttatcggaaa aagcagaggt gatcacaacc gtctgggcat
     4741 tgccctgcaa attggatgtg tccgttttct gggcaccttc ctcaccgata tgaatcatat
     4801 tccttccggc gtccggcatt ttaccgccag acagctcggg attcgtgata tcaccgttct
     4861 tgcagaatac ggtcagaggg aaaatacccg ccgtgagcat gcagcgctga tacgtcagca
     4921 ctatcagtat cgtgaatttg cctggccctg gacatttcgc cttacccgtc ttttatatac
     4981 ccggagctgg ataagcaacg aacgtcctgg cctgcttttc gatctggcga cagggtggct
     5041 tatgcaacat cgtattattc tccccggagc cactacgctg acccggttga tttcagaggt
     5101 aagggaaaag gcgacgttgc gcctgtggaa caaactggca ctgataccgt cagccgaaca
     5161 gcgttcacag ctggagatgc tgctggggcc aactgattgc agccgcctgt ctttactgga
     5221 atcactgaaa aagggccctg tgaccatcag tggtccggcg tttaatgaag caattgaacg
     5281 ctggaaaact ctgaacgatt ttggcctgca tgctgaaaac ctgagtacac tcccggctgt
     5341 gcgcctgaaa aatctaaaag ggccctgtga ccatcagtgg tccggcgttt aatgaagcaa
     5401 ttgaacgctg gaaaactctg aacgattttg gcctgcatgc tgaaaacctg agtacactcc
     5461 cggctgtgcg cctgaaaaat ctcgcacgtt atgctggtat gacttcggtg ttcaatattg
     5521 ccaggatgtc accgcagaaa aggatggcgg ttctggttgc ctttgtcctt gcatgggaaa
     5581 cgctggcgct ggatgatgca ttggacgttc tggacgccat gctggccgtt atcatccgtg
     5641 acgccagaaa gattgggcag aaaaaacggc tccgctcgct gaaggatctg gataaatctg
     5701 cattggcgct cgccagcgca tgttcgtacc tgctgaaaga agaaacaccg gacgaatcga
     5761 ttcgtgctga ggtgttcagc tacatcccaa ggcaaaagct ggctgaaatc atcacgcttg
     5821 tccgtgaaat tgcccggccc tcagacgata attttcatga agaaatggtg gagcagtacg
     5881 ggcgcgttcg tcgtttcctg ccccatctgc tgaataccgt taaattttca tccgcacctg
     5941 ccggggttac cactctgaat gcctgtgact acctcagccg ggagttcagc tcacggcggc
     6001 agttttttga cgacgcacca acggaaatta tcagtcggtc atggaaacgg ctggtgatta
     6061 acaaggaaaa acatatcacc cgcaggggat acacgctctg ctttctcagt aaactgcagg
     6121 atagtctgag gcggagggat gtctacgtta ccggcagtaa ccggtgggga gatcctcgtg
     6181 caagattact acagggtgct gactggcagg caaaccggat taaggtttat cgttctttgg
     6241 ggcacccgac agacccgcag gaagcaataa aatctctggg tcatcagctt gatagtcgtt
     6301 acagacaggt tgctgcacgt ctttgcgaaa atgaggctgt cgaactcgat gtttctggcc
     6361 cgaagccccg gttgacaatt tctcccctcg ccagtcttga tgagccggac agtctgaaac
     6421 gactgagcaa aatgatcagt gatctactcc ctccggtgga tttaacggag ttgctgctcg
     6481 aaattaacgc ccataccgga tttgctgatg agtttttcca tgctagtgaa gccagtgcca
     6541 gagttgatga tctgcccgtc agcatcagcg ccgtgctgat ggctgaagcc tgcaatatcg
     6601 gtctggaacc actgatcaga tcaaatgttc ctgcactgac ccgacaccgg ctgaactgga
     6661 caaaagcgaa ctatctgcgg gctgaaacta tcaccagcgc taatgccaga ctggttgatt
     6721 ttcaggcaac gctgccactg gcacagatat ggggtggagg agaagtggca tctgcagatg
     6781 gaatgcgctt tgttacgcca gtcagaacaa tcaatgccgg accgaaccgc aaatactttg
     6841 gtaataacag agggatcacc tggtacaact ttgtgtccga tcagtattcc ggctttcatg
     6901 gcatcgttat accggggacg ctgagggact ctatctttgt gctggaaggt cttctggaac
     6961 aggagaccgg gctgaatcca accgaaatta tgaccgatac agcaggtgcc agcgaacttg
     7021 tctttggcct tttctggctg ctgggatacc agttttctcc acgcctggct gatgccggtg
     7081 cttcggtttt ctggcgaatg gaccatgatg ccgactatgg cgtgctgaat gatattgcca
     7141 gagggcaatc agatccccga aaaatagtcc ttcagtggga cgaaatgatc cggaccgctg
     7201 gctccctgaa gctgggcaaa gtacaggctt cagtgctggt ccgttcattg ctgaaaagtg
     7261 aacgtccttc cggactgact caggcaatca ttgaagtggg gcgcatcaac aaaacgctgt
     7321 atctgcttaa ttatattgat gatgaagatt accgccggcg cattctgacc cagcttaatc
     7381 ggggagaaag tcgccatgcc gttgccagag ccatctgtca cggtcaaaaa ggtgagataa
     7441 gaaaacgata taccgacggt caggaagatc aactgggcgc actggggctg gtcactaacg
     7501 ccgtcgtgtt atggaacact atttatatgc aggcagccct ggatcatctc cgggcgcagg
     7561 gtgaaacact gaatgatgaa gatatcgcac gcctctcccc gctttgccac ggacatatca
     7621 atatgctcgg ccattattcc ttcacgctgg cagaactggt gaccaaagga catctgagac
     7681 cattaaaaga ggcgtcagag gcagaaaacg ttgcttaacg tgagttttcg ttccactgag
     7741 cgtcagaccc ctatacaggc ataaaaatca gtttctataa gcggctttct tgcacctact
     7801 tggtgatttt ttggatgcgc tgacgttgct cgactttaaa tcaccataga aaaataccct
     7861 ttacttacat aaagatcgat ctggatgtgt atcttgacga ggttcaatac gagcaattgt
     7921 ttttatattt aaggtttctt atcgccgttt tttgcggctc tagaaaaatc aatacaaaag
     7981 atcttgaaag ccacctcgct ccctgggtac acttccgtat aagaactgat cgatgtctaa
     8041 aacggattgt attatggctt ttatctctct atcgaacttg gcagcgaaga ctggaaggat
     8101 aatccagtcg cagtgtgtta cggccacgca aacgaaaaaa agcacgggcg ctgtcgagct
     8161 gggattccgc ttgagcaaga gcgctatgga cgagactggt attaccattg gggatcgtgt
     8221 cgacattctt tacgacaaag aagaaaacca gtggatgatt aagaaaatta gtgaaggtgg
     8281 ctttggtgtg tctgggcaaa aaaacaagca cggcggttac tcatcgtgtg ctgttagatt
     8341 gaccttgcga ccaggttttc ctcgcctttc tgaggatgaa gaggacaaca agaagctact
     8401 ttccgatgat tcattcactc agctaagtga aggtgtaatc gtcttcaggc tggatgatga
     8461 ataggaagga ccaaaaagga aaggcagagt tagtgagcgt cgccaaacaa tcgactaact
     8521 ccgccttctt gtcaacacat cactaccttt ggaagggtgg agtgttatgg gcggcattat
     8581 gttcataaac ttgcattcta ataaagaaaa aatcgtttct tttgagggat gtgaacacga
     8641 cattttctat tctgataggt gcgatgctat tggctttggt ctcttaagtt tatgttttta
     8701 aaaacatatg aggctataca tatgcacgct ttatcaatac taccttcagt tgcccgcgct
     8761 aacctggcaa ctaaattctc gtctcatttg aaggtgatcg agctcttcaa cacgatgcag
     8821 gattcccaag ttgttgtctt gtcatcactg atcgaaggcc atcacctcac cagttctggt
     8881 aacagtgtca aagccgactt tgaggtcact cgcttaccgg cgattatcga gatgttagag
     8941 aaaaaatatt ttttccctat tcgtcatcta aatgtatctg ttaaatctgt aactacagga
     9001 aggatgactg gacaaactgt ctatttcata gagcctgaac acatcgagca acttctcgct
     9061 gatcctgagc ttgtgtttgc taatcaggaa aggagcctct tctttcgttc tctcgaaaaa
     9121 gaagggaaaa atctcggcaa gcttatcgag aaaaaaggta gtgtttctca agccgtctta
     9181 agccttctac atcacgccta caaagataaa cctctttctg acgagatgtg gaaggagata
     9241 gaagataagt tcacccatat gctggatgaa ttgagcgcgg cgtaaaggca agggcggtcg
     9301 ttagaccgcc tttttagtct tctaaaccta attctttgtg atagtttttc agcgtagagt
     9361 aacgatagtt tctgagtatg ttttcatctt tcttgacgtt cgcttctatg tatctgttta
     9421 cgaatttgtt gaaatcttct tgtatttttt ctttgttatc attgatatgg gtaaattcag
     9481 gagttggtat cttataggct cgcgatacat actcttcttt cttaattagc aacgctttgg
     9541 tgtaatccag tcctttctta acaccttctg atcgctccga aacgaatcca aatttatggt
     9601 ttaaatggct tcgtaagggt atcccgaaca ccaatccgtt gagatcaata actacgattc
     9661 cgtagcctct tacttttcca tcattactgc cgtcgagagc ctcttgtagg cctttgttat
     9721 cagcataaaa ctggtctgta agggttctta actgtatgta atcctgtttg ctcatttttg
     9781 atgtccattg gcattttcct gtaaccaatg tacaaaaaaa gcaaagccct ctgtaagaga
     9841 gggctttgct tttctttcgt tgagtcttgg attttttacg tgtggcgaac tctaccgcac
     9901 atctttcttt ggaagcttgt ttgtttaacg tggggctcat tcctaccgcc acttctagat
     9961 ttacttatat caaaaattat atgtgaagtc aatttcgtag ctgttcttga ctggacttac
    10021 tttgccagcg taatcttctc ctgaaacgaa aaaaccaccc tggcaggtgg tttttcgaag
    10081 gttatttaat cctggcagat tatctaaccg tggtaacagt cttgtgcgag acgtgtcacc
    10141 aaatccgtcc ttccagtgta gcctcagtaa ggccaccact tcaagaactc tcaatacatc
    10201 tctcgcacat catgtttacc agtggccgtt gccaatggcg attagtcgtg tctttccggg
    10261 ttggactcaa gtcgatagtt accggaaaag gcgcagcggt cggactgaac ggggggttcg
    10321 tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg
    10381 agataaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga
    10441 gcgcacgagg gagccaccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    10501 ccaccactga t
//
