LOCUS       CP082387               11038 bp    DNA     circular BCT 11-JAN-2022
DEFINITION  Salmonella enterica subsp. enterica serovar Reading strain
            FSIS1701482 plasmid pF18S042-1, complete sequence.
ACCESSION   CP082387
VERSION     CP082387.1
DBLINK      BioProject: PRJNA292666
            BioSample: SAMN07237761
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Reading
  ORGANISM  Salmonella enterica subsp. enterica serovar Reading
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 11038)
  AUTHORS   Li,C., Tyson,G.H., Hsu,C.H., Harrison,L., Strain,E., Tran,T.T.,
            Tillman,G.E., Dessai,U., McDermott,P.F. and Zhao,S.
  TITLE     Long-Read Sequencing Reveals Evolution and Acquisition of
            Antimicrobial Resistance and Virulence Genes in Salmonella enterica
  JOURNAL   Front Microbiol 12, 777817 (2021)
   PUBMED   34867920
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11038)
  CONSRTM   NARMS: The National Antimicrobial Resistance Monitoring System
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2018) Center for Veterinary Medicine, US Food and
            Drug Administration, 8401 Muirkirk Rd, Laurel, MD, USA
REFERENCE   3  (bases 1 to 11038)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2020
            Assembly Method :: SMRTLink Microbial Assembly v. SMRTLink9.0
            Assembly Name                     :: F18S042
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/02/2021 10:27:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,525
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,295
            CDSs (with protein)               :: 4,295
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 50 of 110
            Pseudo Genes (incomplete)         :: 60 of 110
            Pseudo Genes (internal stop)      :: 23 of 110
            Pseudo Genes (multiple problems)  :: 20 of 110
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11038
                     /organism="Salmonella enterica subsp. enterica serovar
                     Reading"
                     /mol_type="genomic DNA"
                     /strain="FSIS1701482"
                     /serovar="Reading"
                     /isolation_source="Animal-Turkey-Young Turkey"
                     /sub_species="enterica"
                     /db_xref="taxon:165302"
                     /plasmid="pF18S042-1"
                     /geo_loc_name="USA:AR"
                     /collection_date="2017"
                     /collected_by="USDA-FSIS"
     ARG             10621..11036
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             10237..11036
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1..837
                     /locus_tag="CED08_022500"
     CDS             1..837
                     /locus_tag="CED08_022500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="UAF56458.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            1143..1385
                     /locus_tag="CED08_022505"
     CDS             1143..1385
                     /locus_tag="CED08_022505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="UAF56459.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="relaxase"
     gene            complement(1417..2067)
                     /gene="tetR(A)"
                     /locus_tag="CED08_022510"
     CDS             complement(1417..2067)
                     /gene="tetR(A)"
                     /locus_tag="CED08_022510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="UAF56462.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            2173..3372
                     /gene="tet(A)"
                     /locus_tag="CED08_022515"
     ARG             2174..3369
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(3339..3527)
                     /locus_tag="CED08_022520"
                     /pseudo=""
     CDS             complement(3339..3527)
                     /locus_tag="CED08_022520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(4125..4409)
                     /locus_tag="CED08_022525"
     CDS             complement(4125..4409)
                     /locus_tag="CED08_022525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="UAF56461.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            4608..5258
                     /locus_tag="CED08_022530"
                     /pseudo=""
     CDS             4608..5258
                     /locus_tag="CED08_022530"
                     /inference="COORDINATES: protein motif:HMM:NF015354.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            5356..5769
                     /locus_tag="CED08_022535"
     CDS             5356..5769
                     /locus_tag="CED08_022535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein B"
                     /protein_id="UAF56463.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="mobilization protein B"
     gene            5814..6158
                     /locus_tag="CED08_022540"
                     /pseudo=""
     CDS             5814..6158
                     /locus_tag="CED08_022540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672138.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family DNA primase"
                     /name="RepB family DNA primase"
     gene            6115..6864
                     /locus_tag="CED08_022545"
     CDS             6115..6864
                     /locus_tag="CED08_022545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001750058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family DNA primase"
                     /protein_id="UAF56451.1"
                     /translation="MNFGGRLPGRWPASTEGREPALVVETSPKNYQAWVKVADAAGGEL
                     RGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATA
                     GPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGD
                     DLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSV
                     QLARAELARAPAPRQRGMDRGGPDFSM"
                     /name="RepB family DNA primase"
     gene            6925..7137
                     /locus_tag="CED08_022550"
     CDS             6925..7137
                     /locus_tag="CED08_022550"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAF56452.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            7139..7345
                     /locus_tag="CED08_022555"
     CDS             7139..7345
                     /locus_tag="CED08_022555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAF56453.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="hypothetical protein"
     gene            7375..8214
                     /locus_tag="CED08_022560"
     CDS             7375..8214
                     /locus_tag="CED08_022560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="UAF56454.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            8201..9052
                     /locus_tag="CED08_022565"
     CDS             8201..9052
                     /locus_tag="CED08_022565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="UAF56455.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="replication protein"
     gene            9360..10175
                     /gene="sul2"
                     /locus_tag="CED08_022570"
     ARG             9361..10172
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             2..834
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          4364..4519
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          7393..8214
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          4609..6158
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6161..6861
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.09e-115"
ORIGIN
        1 atgttcatgc cgcctgtttt tcctgctcat tggcacgttt cgcaacctgt tctcattgcg
       61 gacacctttt ccagcctcgt ttggaaagtt tcattgccag acgggactcc tgcaatcgtc
      121 aagggattga aacctataga agacattgct gatgaactgc gcggggccga ctatctggta
      181 tggcgcaatg ggaggggagc agtccggttg ctcggtcgtg agaacaatct gatgttgctc
      241 gaatatgccg gggagcgaat gctctctcac atcgttgccg agcacggcga ctaccaggcg
      301 accgaaattg cagcggaact aatggcgaag ctgtatgccg catctgagga acccctgcct
      361 tctgcccttc tcccgatccg ggatcgcttt gcagctttgt ttcagcgggc gcgcgatgat
      421 caaaacgcag gttgtcaaac tgactacgtc cacgcggcga ttatagccga tcaaatgatg
      481 agcaatgcct cggaactgcg tgggctacat ggcgatctgc atcatgaaaa catcatgttc
      541 tccagtcgcg gctggctggt gatagatccc gtcggtctgg tcggtgaagt gggctttggc
      601 gccgccaata tgttctacga tccggctgac agagacgacc tttgtctcga tcctagacgc
      661 attgcacaga tggcggacgc attctctcgt gcgctggacg tcgatccgcg tcgcctgctc
      721 gaccaggcgt acgcttatgg gtgcctttcc gcagcttgga acgcggatgg agaagaggag
      781 caacgcgatc tagctatcgc ggccgcgatc aagcaggtgc gacagacgtc atactagata
      841 tcaagcgact tctcctatcc cctgggaaca catcaatctc accggagaat atcgctggcc
      901 aaagccttag cgtaggattc cgccccttcc cgcaaacgac ccctggaagc cccgtagcga
      961 cgcggagagg ggcgagacaa gccaagggcg caggctcgat gcgcagcacg acatagccgg
     1021 ttctcgcaag gacgagaatt tccctgcggt gcccctcaag tgtcaatgaa agtttccaac
     1081 gcgagccatt cgcgagagcc ttgagtccac gctagatcta tctcatctgc gcaaggcaga
     1141 acgtgaagac ggccgccctg gacctcgccc gcgagcgcca ggcgcacgag gccggcgcgc
     1201 ggacccgcgc cacggcccac gagcggacgc cgcagcagga gcgccagaag gccgccagag
     1261 aggccgagcg cggccgtgag gcttggacgc tagggcaggg catgaaaaag cccgtagcgg
     1321 gctgctacgg gcgtctgacg cggtggaaag ggggagggga tgttgtctac atggctctgc
     1381 tgtagtgagt gggttgcgct ccggcagcgg tcctgatcaa tcgtcaccct ttctcggtcc
     1441 ttcaacgttc ctgacaacga gcctcctttt cgccaatcca tcgacaatca ccgcgagtcc
     1501 ctgctcgaac gctgcgtccg gaccggcttc gtcgaaggcg tctatcgcgg cccgcaacag
     1561 cggcgagagc ggagcctgtt caacggtgcc gccgcgctcg ccggcatcgc tgtcgccggc
     1621 ctgctcctca agcacggccc caacagtgaa gtagctgatt gtcatcagcg cattgacggc
     1681 gtccccggcc gaaaaacccg cctcgcagag gaagcgaagc tgcgcgtcgg ccgtttccat
     1741 ctgcggtgcg cccggtcgcg tgccggcatg gatgcgcgcg ccatcgcggt aggcgagcag
     1801 cgcctgcctg aagctgcggg cattcccgat cagaaatgag cgccagtcgt cgtcggctct
     1861 cggcaccgaa tgcgtatgat tctccgccag catggcttcg gccagtgcgt cgagcagcgc
     1921 ccgcttgttc ctgaagtgcc agtaaagcgc cggctgctga acccccaacc gttccgccag
     1981 tttgcgtgtc gtcagaccgt ctacgccgac ctcgttcaac aggtccaggg cggcacggat
     2041 cactgtattc ggctgcaact ttgtcatgct tgacacttta tcactgataa acataatatg
     2101 tccaccaact tatcagtgat aaagaatccg cgcgttcaat cggaccagcg gaggctggtc
     2161 cggaggccag acgtgaaacc caacagaccc ctgatcgtaa ttctgagcac tgtcgcgctc
     2221 gacgctgtcg gcatcggcct gattatgccg gtgctgccgg gcctcctgcg cgatctggtt
     2281 cactcgaacg acgtcaccgc ccactatggc attctgctgg cgctgtatgc gttgatgcaa
     2341 tttgcctgcg cacctgtgct gggcgcgctg tcggatcgtt tcgggcggcg gccggtcttg
     2401 ctcgtctcgc tggccggcgc tgctgtcgac tacgccatca tggcgacggc gcctttcctt
     2461 tgggttctct atatcgggcg gatcgtggcc ggcatcaccg gggcgactgg ggcggtagcc
     2521 ggcgcttata ttgccgatat cactgatggc gatgagcgcg cgcggcactt cggcttcatg
     2581 agcgcctgtt tcgggttcgg gatggtcgcg ggacctgtgc tcggtgggct gatgggcggt
     2641 ttctcccccc acgctccgtt cttcgccgcg gcagccttga acggcctcaa tttcctgacg
     2701 ggctgtttcc ttttgccgga gtcgcacaaa ggcgaacgcc ggccgttacg ccgggaggct
     2761 ctcaacccgc tcgcttcgtt ccggtgggcc cggggcatga ccgtcgtcgc cgccctgatg
     2821 gcggtcttct tcatcatgca acttgtcgga caggtgccgg ccgcgctttg ggtcattttc
     2881 ggcgaggatc gctttcactg ggacgcgacc acgatcggca tttcgcttgc cgcatttggc
     2941 attctgcatt cactcgccca ggcaatgatc accggccctg tagccgcccg gctcggcgaa
     3001 aggcgggcac tcatgctcgg aatgattgcc gacggcacag gctacatcct gcttgccttc
     3061 gcgacacggg gatggatggc gttcccgatc atggtcctgc ttgcttcggg tggcatcgga
     3121 atgccggcgc tgcaagcaat gttgtccagg caggtggatg aggaacgtca ggggcagctg
     3181 caaggctcac tggcggcgct caccagcctg acctcgatcg tcggacccct cctcttcacg
     3241 gcgatctatg cggcttctat aacaacgtgg aacgggtggg catggattgc aggcgctgcc
     3301 ctctacttgc tctgcctgcc ggcgctgcgt cgcgggcttt ggagaaattc ttcaaattcc
     3361 cgttgcacat agcccggcaa ttcctttccc tgctctgcca taagcgcagc gaatgccggg
     3421 taatactcgt caacgatctg atagagaagg gtttgctcgg gtcggtggct ctggtaacga
     3481 ccagtatccc gatcccggct ggccgtcctg gccgccacat gaggcatgtt ccgcgtcctt
     3541 gcaatactgt gtttacatac agtctatcgc ttagcggaaa gttcttttac cctcagccga
     3601 aatgcctgcc gttgctagac attgccagcc agtgcccgtc actcccgtac taactgtcac
     3661 gaacccctgc aataactgtc acgcccccct gcaataactg tcacgaaccc ctgcaataac
     3721 tgtcacgccc ccctgcaata actgtcacga acccctgcaa taactgtcac gcccccaaac
     3781 ctgcaaaccc agcaggggcg ggggctggcg gggtgttgga aaaatccatc catgattatc
     3841 taagaataat ccactaggcg cggttatcag cgcccttgtg gggcgctgct gcccttgccc
     3901 aatatgcccg gccagaggcc ggatagctgg tctattcgct gcgctaggct acacaccgcc
     3961 ccaccgctgc gcggcagggg gaaaggcggg caaagcccgc taaaccccac accaaacccc
     4021 gcagaaatac gctggagcgc ttttagccgc tttagcggcc tttcccccta cccgaagggt
     4081 gggggcgcgt gtgcagcccc gcagggcctg tctcggtcga tcattcagcc cggctcatcc
     4141 ttctggcgtg gcggcagacc gaacaaggcg cggtcgtggt cgcgttcaag gtacgcatcc
     4201 attgccgcca tgagccgatc ctccggccac tcgctgctgt tcaccttggc caaaatcatg
     4261 gcccccacca gcaccttgcg ccttgtttcg ttcttgcgct cttgctgctg ttcccttgcc
     4321 cgcacccgct gaatttcggc attgattcgc gctcgttgtt cttcgagctt ggccagccga
     4381 tccgccgcct tgttgctccc cttaaccatc ttgacacccc attgttaatg tgctgtctcg
     4441 taggctatca tggaggcaca gcggcggcaa tcccgaccct actttgtagg ggagggcgca
     4501 cttaccggtt tctcttcgag aaactggcct aacggccacc cttcgggcgg tgcgctctcc
     4561 gagggccatt gcatggagcc gaaaagcaaa agcaacagcg aggcagcatg gcgatttatc
     4621 accttacggc gaaaaccggc agcaggtcgg gcggccaatc ggccagggcc aaggccgact
     4681 acatccagcg cgaaggcaag tatgcccgcg acatggatga agtcttgcac gccgaatccg
     4741 ggcacatgcc ggagttcgtc gagcggcccg ccgactactg ggatgctgcc gacctgtatg
     4801 aacgcgccaa tgggcggctg ttcaaggagg tcgaatttgc cctgccggtc gagctgaccc
     4861 tcgaccagca gaaggcgctg gcgtccgagt tcgcccagca cctgaccggt gccgagcgcc
     4921 tgccgtatac gctggccatc catgccggtg gcggcgagaa cccgcactgc cacctgatga
     4981 tctccgagcg gatcaatgac ggcatcgagc ggcccgccgc tcagtggttc aagcggtaca
     5041 acggcaagac cccggagaag ggcggggcac agaagaccga agcgctcaag cccaaggcat
     5101 ggcttgagca gacccgcgag gcatgggccg accatgccaa ccgggcatta gagcgggctg
     5161 gccacgacgc ccgcattgac cacagaacac ttgaggcgca gggcatcgag cgcctgcccg
     5221 gtgttcacct ggggccgaac gtggtggaga tggaaggccg gggcatccgc accgaccggg
     5281 cagacgtggc cctgaacatc gacaccgcca acgcccagat catcgactta caggaatacc
     5341 gggaggcaat agaccatgaa cgcaatcgac agagtgaaga aatccagagg catcaacgag
     5401 ttagcggagc agatcgaacc gctggcccag agcatggcga cactggccga cgaagcccgg
     5461 caggtcatga gccagaccca gcaggccagc gaggcgcagg cggcggagtg gctgaaagcc
     5521 cagcgccaga caggggcggc atgggtggag ctggccaaag agttgcggga ggtagccgcc
     5581 gaggtgagca gcgccgcgca gagcgcccgg agcgcgtcgc gggggtggca ctggaagcta
     5641 tggctaaccg tgatgctggc ttccatgatg cctacggtgg tgctgctgat cgcatcgttg
     5701 ctcttgctcg acctgacgcc actgacaacc gaggacggct cgatctggct gcgcttggtg
     5761 gcccgatgaa gaacgacagg actttgcagg ccataggccg acagctcaag gccatgggct
     5821 gtgagcgctt cgatatcggc gtcagggacg ccaccaccgg ccagatgatg aaccgggaat
     5881 ggtcagccgc cgaagtgctc cagaacacgc catggctcaa gcggatgaat gcccagggca
     5941 atgacgtgta tatcaggccc gccgagcagg agcggcatgg tctggtgctg gtggacgacc
     6001 tcagcgagtt tgacctggat gacatgaaag ccgagggccg ggagcctgcc ctggtagtgg
     6061 aaaccagccc gaagaactat caggcatggg tcaaggtggc cgacgccgca ggcggtgaac
     6121 ttcgggggca gattgcccgg acgctggcca gcgagtaccg agggccggga gcctgccctg
     6181 gtagtggaaa ccagcccgaa gaactatcag gcatgggtca aggtggccga cgccgcaggc
     6241 ggtgaacttc gggggcagat tgcccggacg ctggccagcg agtacgacgc cgacccggcc
     6301 agcgccgaca gccgccacta tggccgcttg gcgggcttca ccaaccgcaa ggacaagcac
     6361 accacccgcg ccggttatca gccgtgggtg ctgctgcgtg aatccaaggg caagaccgcc
     6421 accgctggcc cggcgctggt gcagcaggct ggccagcaga tcgagcaggc ccagcggcag
     6481 caggagaagg cccgcaggct ggccagcctc gaactgcccg agcggcagct tagccgccac
     6541 cggcgcacgg cgctggacga gtaccgcagc gagatggccg ggctggtcaa gcgcttcggt
     6601 gatgacctca gcaagtgcga ctttatcgcc gcgcagaagc tggccagccg gggccgcagt
     6661 gccgaggaaa tcggcaaggc catggccgag gccagcccag cgctggcaga gcgcaagccc
     6721 ggccacgaag cggattacat cgagcgcacc gtcagcaagg tcatgggtct gcccagcgtc
     6781 cagcttgcgc gggccgagct ggcacgggca ccggcacccc gccagcgagg catggacagg
     6841 ggcgggccag atttcagcat gtagtgcttg cgttggtact cacgcctgtt atactatgag
     6901 tactcacgca cagaaggggg ttttatggaa tacgaaaaaa gcgcttcagg gtcggtctac
     6961 ctgatcaaaa gtgacaaggg ctattggttg cccggtggct ttggttatac gtcaaacaag
     7021 gccgaggctg gccgcttttc agtcgctgat atggccagcc ttaaccttga cggctgcacc
     7081 ttgtccttgt tccgcgaaga caagcctttc ggccccggca agtttctcgg tgactgatat
     7141 gaaagaccaa aaggacaagc agaccggcga cctgctggcc agccctgacg ctgtacgcca
     7201 agcgcgatat gccgagcgca tgaaggccaa agggatgcgt cagcgcaagt tctggctgac
     7261 cgacgacgaa tacgaggcgc tgcgcgagtg cctggaagaa ctcagagcgg cgcagggcgg
     7321 gggtagtgac cccgccagcg cctaaccacc aactgcctgc aaaggaggca atcaatggct
     7381 acccataagc ctatcaatat tctggaggcg ttcgcagcag cgccgccacc gctggactac
     7441 gttttgccca acatggtggc cggtacggtc ggggcgctgg tgtcgcccgg tggtgccggt
     7501 aaatccatgc tggccctgca actggccgca cagattgcag gcgggccgga tctgctggag
     7561 gtgggcgaac tgcccaccgg cccggtgatc tacctgcccg ccgaagaccc gcccaccgcc
     7621 attcatcacc gcctgcacgc ccttggggcg cacctcagcg ccgaggaacg gcaagccgtg
     7681 gctgacggcc tgctgatcca gccgctgatc ggcagcctgc ccaacatcat ggccccggag
     7741 tggttcgacg gcctcaagcg cgccgccgag ggccgccgcc tgatggtgct ggacacgctg
     7801 cgccggttcc acatcgagga agaaaacgcc agcggcccca tggcccaggt catcggtcgc
     7861 atggaggcca tcgccgccga taccgggtgc tctatcgtgt tcctgcacca tgccagcaag
     7921 ggcgcggcca tgatgggcgc aggcgaccag cagcaggcca gccggggcag ctcggtactg
     7981 gtcgataaca tccgctggca gtcctacctg tcgagcatga ccagcgccga ggccgaggaa
     8041 tggggtgtgg acgacgacca gcgccggttc ttcgtccgct tcggtgtgag caaggccaac
     8101 tatggcgcac cgttcgctga tcggtggttc aggcggcatg acggcggggt gctcaagccc
     8161 gccgtgctgg agaggcagcg caagagcaag ggggtgcccc gtggtgaagc ctaagaacaa
     8221 gcacagcctc agccacgtcc ggcacgaccc ggcgcactgt ctggcccccg gcctgttccg
     8281 tgccctcaag cggggcgagc gcaagcgcag caagctggac gtgacgtatg actacggcga
     8341 cggcaagcgg atcgagttca gcggcccgga gccgctgggc gctgatgatc tgcgcatcct
     8401 gcaagggctg gtggccatgg ctgggcctaa tggcctagtg cttggcccgg aacccaagac
     8461 cgaaggcgga cggcagctcc ggctgttcct ggaacccaag tgggaggccg tcaccgctga
     8521 tgccatggtg gtcaaaggta gctatcgggc gctggcaaag gaaatcgggg cagaggtcga
     8581 tagtggtggg gcgctcaagc acatacagga ctgcatcgag cgcctttgga aggtatccat
     8641 catcgcccag aatggccgca agcggcaggg gtttcggctg ctgtcggagt acgccagcga
     8701 cgaggcggac gggcgcctgt acgtggccct gaaccccttg atcgcgcagg ccgtcatggg
     8761 tggcggccag catgtgcgca tcagcatgga cgaggtgcgg gcgctggaca gcgaaaccgc
     8821 ccgcctgctg caccagcggc tgtgtggctg gatcgacccc ggcaaaaccg gcaaggcttc
     8881 catagatacc ttgtgcggct atgtctggcc gtcagaggcc agtggttcga ccatgcgcaa
     8941 gcgccgccag cgggtgcgcg aggcgttgcc ggagctggtc gcgctgggct ggacggtaac
     9001 cgagttcgcg gcgggcaagt acgacatcac ccggcccaag gcggcaggct gacccccccc
     9061 actctattgt aaacaagaca tttttatctt ttatattcaa tggcttattt tcctgctaat
     9121 tggtaatacc atgaaaaata ccatgctcag aaaaggctta acaatatttt gaaaaattgc
     9181 ctactgagcg ctgccgcaca gctccatagg ccgctttcct ggctttgctt ccagatgtat
     9241 gctcttctgc tcctgcagct aatggatcac cgcaaacagg ttactcgcct ggggattccc
     9301 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
     9361 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
     9421 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
     9481 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
     9541 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
     9601 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     9661 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     9721 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     9781 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     9841 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     9901 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     9961 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
    10021 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
    10081 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
    10141 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
    10201 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
    10261 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
    10321 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
    10381 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
    10441 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
    10501 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
    10561 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
    10621 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
    10681 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
    10741 tgcggctcga ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga
    10801 acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg
    10861 gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg
    10921 cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag
    10981 cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttg
//
