LOCUS       CP082151                3982 bp    DNA     circular BCT 19-OCT-2021
DEFINITION  Enterobacter cloacae complex sp. strain ECL42 plasmid pIMP-042004,
            complete sequence.
ACCESSION   CP082151
VERSION     CP082151.1
DBLINK      BioProject: PRJNA757181
            BioSample: SAMN20834670
KEYWORDS    .
SOURCE      Enterobacter cloacae complex sp
  ORGANISM  Enterobacter cloacae complex sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Zhang,X.
  TITLE     Molecular characterisation of NDM-1-Producing Carbapenem-Resistant
            E. cloacae complex From a Three Tertiary Hospitals in
            Chongqing,China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Zhang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2021) Department of Microbiology, Yongchuan
            Hospital of Chongqing Medical University, Xuanhua Road, Yongchuan
            District, Chongqing, Chongqing 402106, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 338.0x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/26/2021 15:06:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,389
            CDSs (total)                      :: 5,269
            Genes (coding)                    :: 5,113
            CDSs (with protein)               :: 5,113
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 33 of 156
            Pseudo Genes (incomplete)         :: 117 of 156
            Pseudo Genes (internal stop)      :: 25 of 156
            Pseudo Genes (multiple problems)  :: 16 of 156
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Enterobacter cloacae complex sp."
                     /mol_type="genomic DNA"
                     /strain="ECL42"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:2027919"
                     /plasmid="pIMP-042004"
                     /geo_loc_name="China: Jiamusi, Heilongjiang Province"
                     /collection_date="2016-09-23"
     gene            127..678
                     /locus_tag="K6966_26910"
     CDS             127..678
                     /locus_tag="K6966_26910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011977834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QZS50316.1"
                     /translation="MVLDLSNTEMLLLIHLISFIHFADTPAFPSISTLAARINQHPRTV
                     QRTINKLISKGFLKRKIRAKSVNDKGLSNLYSVEPLIEKLFKSIDNKPEDHSSSNDQQG
                     VGEMKTIDQLVRIAAAGGGLRINASTKTVDQLVRIAAAAGNSMNPNKAQLVLFDLESKS
                     TDQLVRIAAAGKGTVLFELE"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1923..2354)
                     /locus_tag="K6966_26915"
     CDS             complement(1923..2354)
                     /locus_tag="K6966_26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QZS50315.1"
                     /translation="MARVSISEAARLARVSRPTIYKLLKSGELSYTSVVKHGKPVKIID
                     TSELMRVFGALEVVSGSKSDTVKFDVVSTPVNSDFLQSLQHQITLLQAENAGLKDAVNA
                     RDEHISSLRQAMQLLEHKQSASDPQNVPAKSWWQFWKSH"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2354..2545)
                     /locus_tag="K6966_26920"
     CDS             complement(2354..2545)
                     /locus_tag="K6966_26920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QZS50317.1"
                     /translation="MTKQQKTALNMAKFIQAQSLLLLEKLNELDLDTEADLCEKLHEDA
                     EQLFRTLAVRLNDFQEDL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3226..3564)
                     /gene="mazF"
                     /locus_tag="K6966_26925"
     CDS             complement(3226..3564)
                     /gene="mazF"
                     /locus_tag="K6966_26925"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011977836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="QZS50313.1"
                     /translation="MTTYCPARGDVILLDFNPQSGHEQAGKRPALVVSDDLFNQVTGFA
                     VVCPITNQQKGYPFEVSVDGAKKTTGVILADQVKSLDWKARAARTVDSVSDETVTAVVG
                     MVSKIIKA"
                     /name="mazF"
     gene            complement(3561..3791)
                     /locus_tag="K6966_26930"
     CDS             complement(3561..3791)
                     /locus_tag="K6966_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011977835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="QZS50314.1"
                     /translation="MTTAIGKWGNGAALRIPQPFMKQLGLSIGDAVNFCVTERNLVITK
                     AGPTLEELLGQCTAENRHHEYFCDSQGKEML"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     Typing          2721..3062
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.354"
                     /coverage="84.0"
                     /evalue="8.11e-130"
ORIGIN
        1 ttgaataaca aagaaaaaaa aataaaacgc aatcagatac cagtaatact ggcaagcaga
       61 tggggttaca gcattcttga ggaagctgga tggacgacta tccctaattt actacttgaa
      121 aagcagatgg ttcttgactt aagcaacaca gaaatgttgt tactcattca ccttatctcg
      181 tttattcact ttgcagatac tcctgcattc ccaagcattt ccacactggc agctaggatc
      241 aaccagcatc caagaacagt gcaacggacc ataaacaaat taatatctaa aggttttcta
      301 aagcggaaga ttcgcgctaa atcagttaat gataaaggtt tgagtaatct ctacagcgta
      361 gagcctttaa tagaaaagct atttaaatca atagataata aacctgaaga ccatagtagc
      421 tctaatgatc aacaaggagt cggagaaatg aaaacaatag accagcttgt acggattgca
      481 gcagctgggg gcggcttgcg cataaatgcc tcaacaaaga cggtagacca gctagtgcgg
      541 attgcagcag cagcaggtaa ctcaatgaat ccaaacaaag cacaactagt gctttttgat
      601 ctggaatcaa aatctacaga ccaacttgtt cggatcgctg ctgccggaaa aggaacggta
      661 ctatttgaat tggaatgaaa agtaaaaaga attaacggaa tggcactaaa ccgacactga
      721 tttaccataa cgtctcatta cgcgcacccg taaagcgcgc tcagcgcgtt ttacgggtga
      781 aggcgatgaa aaccacagct tacccggcga aaaccccgca aaacggcgca gatgaattct
      841 gagagggtga atggctatgc atccggatgc atggtgcggg ttttgacaga aaacagttgg
      901 tgtaagggcg gttttgatcg gttttccggc taggtaattc agttcaataa cgcctaccgg
      961 ttccgttccc tgccataaaa ggtcagttcc ccgttcctga tttgcaaaac gccccgtgct
     1021 aacttcccgg ccacggaaga acggtgcatc tcatcatgtc tccgtcggat ctcctcagcc
     1081 gtcatcggcc tgatctccag atccaaaaac tctctcttac actccgcgcc cagaaatttc
     1141 aggtcatcga taagggtcgc aaaccattcg ttcaccgttt tatctccaca ctagtaagcc
     1201 attcagataa cagtctggca caggctggcg aaagaaagcg gtgaacgggc acgcaattta
     1261 ttgcgggagc gggaggcgct ggccgggtgt cggggcggcg agccctgacc gggtttcaaa
     1321 ggggaaccct ttggcacgcg actgtataca gtcggtaacg tgccttactc tggaaccggc
     1381 agttgcattg aattgctcta aatttcgtta aattgcacta aattgcatta gttctcgccg
     1441 atgcacgaat ggattgcatt aagttgcact gagttgcatt accgctgaca ggtcttttat
     1501 ccattacacc gcgcagggag agaagctgat atagcgtcac atacacagaa aaaaggtata
     1561 ccaggctcaa tcccttatcc tgtgcagcat tgcagccctc agagcagtgt ttcgggtata
     1621 ccatgccagt caacacctgg tataccgcct acaggcctta tcacgactgg ctttaacggc
     1681 ataaaaacag atgcatgtat accatttcat acatgagggg gtataccccc ggaagccttg
     1741 ccagcaaagg cttccggctg agcagtgtat actgcgtggg gtgtgacacg attttttggc
     1801 ctttatcagc catcagaaat aaacatgcgg gtaaagcggt cagatgggaa agtataaaca
     1861 tccatatcag gtgatcgcct gacagctggt cgaacgctat gaccggaatg cgtggtaata
     1921 actcaatggg atttccagaa ttgccaccag gattttgcag gaacattctg cgggtcagaa
     1981 gcgctctgtt tatgctccag aagttgcatg gcctgtcgaa gtgaactgat atgctcatcc
     2041 cgggcattca ccgcatcttt gagcccggca ttctccgcct gtaaaagcgt tatctgatgc
     2101 tgtaaagact gtaaaaaatc actgttaacc ggcgtagaca caacgtcaaa ttttacagtg
     2161 tcacttttac taccacttac aacctcaaga gcaccaaaaa cgcgcataag ctcagacgta
     2221 tcaatgattt ttacgggttt tccatgcttt acaactgagg tgtaactgag ttcgccagac
     2281 ttaagtagct tgtaaatagt cgggcggctg acacgggcaa gccgtgccgc ttcgctaatg
     2341 gatacacggg ccattacaaa tcctcttgaa aatcattcag acgaacagcc agagtgcgaa
     2401 agagctgttc cgcatcctca tgcagttttt cgcacaggtc ggcctcagta tcgagatcca
     2461 gttcgttgag cttctccagc aaaagtagag attgcgcctg aatgaattta gccatgttca
     2521 atgccgtttt ctgttgcttt gtaatcacag cgtcagtgct ccaagtcatc tccggttctt
     2581 ttccgcttcc tcgaccgttc gctcgctacg ctcggtcact tgtctgcggc gagcggacgg
     2641 agcttactaa gagacaaata acgtagacgg taggcgtaac gaaaagccag aacaagcgca
     2701 ataaggccac gcccggccag ggcgtttttc cataggcttc gcccccctga caagcgtcac
     2761 aaaacagacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
     2821 ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
     2881 ttccgctgtt atggccgcgg tttctcattc cacgcctgac actcggctcc gggaaggcag
     2941 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgactac cgcgctaatt
     3001 cggtaactat caacttgagt ccaacccgaa taagcatgcc aaagcaccgc tggcagtagc
     3061 catcggtgac tctatggggg gaaagaaaat cttgattgat gtaacagtaa acagttacac
     3121 tcaatgaaca atattggtta ctgttcccct cagagcagtc accgaaggtc gaagatgctg
     3181 gtaacatctc cgacctgaaa agaccggctt atggccggtc tttttttatg ccttaataat
     3241 ttttgaaacc ataccgacaa ccgcagttac agtctcatcg gaaacactat caactgttcg
     3301 ggcagctctt gccttccagt ctaaagactt cacttgatca gccaaaataa cccctgtcgt
     3361 tttctttgca ccatcaacag aaacctcaaa cggatatcct ttttgttgat tggttatagg
     3421 acaaacaaca gcaaacccag tgacctgatt aaacaaatca tcagaaacaa cgagagcagg
     3481 ccttttcccg gcctgctcat gccctgattg agggttgaaa tctaaaagaa tgacatcgcc
     3541 tctggctgga caatatgtcg tcacagcatc tccttacctt gagagtcaca aaaatattcg
     3601 tgatggcgat tttctgcagt gcactgtccg agcagttctt caagcgtcgg gccagcttta
     3661 gtgattacta aatttctttc ggtgacgcag aaattaacag catcaccaat actgagccca
     3721 agctgcttca tgaacggctg cgggatacgc aaagcagcgc cattacccca tttaccgata
     3781 gccgtagtca taatacctcc aaagcagatg gataaacaat gtgtaaacac tatatgtatt
     3841 gtatagtctt taagttcagt ttgaagggtg gcaccagtgc caccctaaaa aactgtaact
     3901 acttaaacaa ataagtacct tatttgttta agtagttaca ctgcgataaa aaaaattttt
     3961 ttgatcaagg tgagttaaac aa
//
