LOCUS       CP077391                5153 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1375 plasmid unnamed2, complete
            sequence.
ACCESSION   CP077391
VERSION     CP077391.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357517
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1124.50x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:33:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,145
            CDSs (total)                      :: 5,022
            Genes (coding)                    :: 4,841
            CDSs (with protein)               :: 4,841
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 84 of 181
            Pseudo Genes (incomplete)         :: 91 of 181
            Pseudo Genes (internal stop)      :: 45 of 181
            Pseudo Genes (multiple problems)  :: 35 of 181
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1375"
                     /culture_collection="FDA:FDAARGOS 1375"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            complement(1..438)
                     /locus_tag="I6L05_25695"
     CDS             complement(1..438)
                     /locus_tag="I6L05_25695"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QXC06293.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="entry exclusion protein 1"
     gene            698..2263
                     /locus_tag="I6L05_25700"
     CDS             698..2263
                     /locus_tag="I6L05_25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-10"
                     /protein_id="QXC06294.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCSFIDKNKLNTINE
                     VLGI"
                     /name="colicin-10"
     gene            complement(2260..2601)
                     /locus_tag="I6L05_25705"
     CDS             complement(2260..2601)
                     /locus_tag="I6L05_25705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="QXC06295.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            2649..2786
                     /locus_tag="I6L05_25710"
     CDS             2649..2786
                     /locus_tag="I6L05_25710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QXC06289.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(3628..3822)
                     /locus_tag="I6L05_25715"
     CDS             complement(3628..3822)
                     /locus_tag="I6L05_25715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QXC06290.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4106..4429
                     /locus_tag="I6L05_25720"
     CDS             4106..4429
                     /locus_tag="I6L05_25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="QXC06291.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="nikA protein"
     gene            complement(4654..5073)
                     /locus_tag="I6L05_25725"
     CDS             complement(4654..5073)
                     /locus_tag="I6L05_25725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QXC06292.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     Typing          3858..4097
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.6e-123"
     Typing          complement(3070..3599)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.59"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          4107..4426
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.11e-62"
ORIGIN
        1 tcagtgaccg aacagcctgg accagaatcc ccgtttcttt tcctgctcca gagcctgcct
       61 gagctgggct atctcattct gtagctgttt ccgttctgct tcctggcgcc ggcgctccgt
      121 atcctgggct tgtttatcct caagcatcag cgtcaggcat tgtttcagct cattcagttc
      181 ccggagaatg cgttctgtct gctgatcatg aggtttttct gagtgtcctt gagtgtgcct
      241 ttctggtgtc tcattccgct ttaattcgcc gtatgcccgg atcagttcac tggtttcaaa
      301 ctcccgtcgt ccgtcctggc tggtacggta actgacaaga cctgagcgca tatcacggta
      361 aaactgtgaa cgcgatcttc cagtcatttt cagtgcgtcc cggagtgtgt gccatgccat
      421 aaagttacag tgtcccatag gtgtctcatc tcatggtttc agtaaaacat aatgaggtct
      481 gagaacggta atgtttgtgc tggtttttgt ggcatcgggt ggaaatagca cgtggtgtgg
      541 gtggctgttt ttttgatcgt tttcacaaaa atgaaaatcc acagggttga cagggaaaat
      601 gcagcggcgt aattttaaac tgtatataaa accagtgatt atatatacag tatattgctt
      661 ttaactattt gtttttaatg tcaaagagga ttttataatg gaaacagctg tagcgtacta
      721 taaagatggt gttccttatg atgataaggg gcaggtaatc attactcttt tgaatggtaa
      781 tccagacggg agtggctctg gcggtggtgg tggaactgga ggtagcaaaa gtgaaagttc
      841 tgcagccatt catgccacag ctaaatggtc tactgctcaa ttgaagaaaa cgcaggcaga
      901 acaggctgcc cgagcaaaag ctgccgcaga agcacaggct aaagcaaaag caaaccggga
      961 tgcgctgact caacatctga aggatattgt gaatgaggcg cttcgccata attccactca
     1021 tccggaggtt attgacctgg ctcatgccaa taatgcagcg atgcaggcag aagcagagcg
     1081 gttgcgcctt gcaaaagcag aagaaaaagc ccgtaaagaa gcggaagctg cggaaaaggc
     1141 ttttcaggaa gcagaacaac gacgtaaaga gatagagaag gagcaggctg aaacagaacg
     1201 ccaattgaaa ctggctgagg atgaagagaa acgcctggca gcattgagtg aagaggcccg
     1261 ggctgtggag gtggcacaaa aaaatcttgc tgctgcacaa tctgagctgg cgaaagtgga
     1321 tgaagagatt aatacgctca atacccgttt aagctccagt attcatgccc gtgatgcaga
     1381 aacgaatacg ctgtccggaa aacgaaatga gttggatcag gcatctgcta aatataaaga
     1441 actggatgaa agggttaaac ttttatctcc tagggcgaat gatccgcttc agagccgtcc
     1501 tttttttgag gccaccagac tacgggcgag agctggtgat gagatggagg aaaaacaaaa
     1561 gcaggtaaca gcatcagaaa cgcgtcttaa ccagattagc tctgagataa atggaatcca
     1621 agaggctatt tctcaggcta ataataagcg aagtacagca gtttcacgta ttcatgatgc
     1681 tgaagataat ttgaaaacag cacagactaa tctcctgaac tcgcagatta aggatgctgt
     1741 ggatgcaaca gttagctttt atcaaacgct atctgaaaaa tatggtgaaa aatattcaaa
     1801 aatggcacag gaacttgctg ataaatctaa aggtaagaaa atcagcaatg tgaatgaagc
     1861 tctagctgct tttgagaaat acaaggatgt attaaataag aaattcagca aagcagaccg
     1921 tgatgcaatt ttcaatgcac tggaggcggt taagtatgaa gactgggcta agcatttaga
     1981 tcagtttgcc aagtacttga agattacggg acatgtttct tttggatatg atgtggtatc
     2041 tgatatccta aaaattaagg atacaggtga ctggaagccg ctatttctta cattagagaa
     2101 gaaagctgta gatgctggag ttagttatgt tgttgtttta ctttttagtg tgcttgctgg
     2161 aactacatta ggtatctggg ggattgctat tgttacaggc attctatgtt cctttattga
     2221 taagaataaa cttaatacta taaatgaggt gttgggtatt taattattct ttattgatat
     2281 aaacagtcct agcaatccaa atgggattgc taggaccaaa caaagtagat tgtatagcat
     2341 aaataggttt aattttgcca cgggggcatt atttaggttt tttctttttc tgaaaaaatc
     2401 tttctttatg aacttaaaaa atatgtgttc aatagcatat tttgaatatg ggcatagaat
     2461 agtgcttatc actattgcgt atagcatctt atctgacgct aggaaataat acccttcgtt
     2521 gttttttgtt ataaggtata tatatataag tgcgcagtat aggccaaaca aaatattttt
     2581 tatgtagtat cttaaactca taaattaaac ctcgttatat attcttttca ttttataagg
     2641 atcgagttat gaggaaaaga ttttttgtgg gaatattcgc tataaacctc cttgttggat
     2701 gtcaggctaa ctatatacgt gatgttcagg gagggactgt ggctccatca tcttcttcta
     2761 aattgacggg gatctctgtt cagtagaaaa gatcaaagga tcttcttgag atcctttttt
     2821 tctgcgcgta atctgctgcc tgtaaacgaa aaaaccgcct ggggaggcgg tttgatcgaa
     2881 ggttaagtca gttggggaac tgcttaaccg aggtaactgg cttcagcaga gcgcagatac
     2941 caaatactgt ccttccagtg tagccgtagt cgggccacta cttcaagaac tctgttgcac
     3001 catttgcgcc atcatcgctc tgctaaccaa gttaccaacg gctgctgcca gtggcgctaa
     3061 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3121 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgagccgag
     3181 ataccaacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3241 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3301 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tggcttgagc gtcgattttt
     3361 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc tgcggcgctg gcttcttccg
     3421 gtgctttgct ttttgctcac atgttctttc cggctttatc ccctgattct gtggataacc
     3481 gtattaccgc ttttgagtga gctgacaccg ctcgccgcag tcgaacgtcc gagcgtagcg
     3541 agtcagtgag cgaggaagcg gaagagcgcc ttatgtgaca ttttctcctt acgctctgtt
     3601 gtgccgttcg gcatcctgtc ctgagcgtta tctctctgtg ctattttcta cttcaaagcg
     3661 tgtctggatg ctgttctgga gttcttctgc gagttcgtgc agcttctcac acatggtagc
     3721 ctgttcgtca gcatcgagtg cgtccagctt ttcgagcagc gtcaggcttt ggctttttat
     3781 gaatcctgcc atgttgagta cggcttgctg ctgcttgttc atcttttcgt tttctccgtt
     3841 ctgtctgtca tctgcgttgt gtgattatat cgcgtaccac ttttcgactg ttttaccgcc
     3901 ggtattctgc cgtctgacgc tttgacgggt atttctgcct gacaatactg tcactgccaa
     3961 aaaactgccg tgcctttgtc ggtaattcga acttgctgac aggacaggat gtgtaattgt
     4021 tataccgcgc atacatgcac gctattacaa ttaccctggt cagggctttg ccccgacacc
     4081 catgtcagat acggagccat gttttatgac aaaacgaagt ggaagtaata cgcgcaggcg
     4141 ggctatcagt cgccctgttc gtctgacggc agaagaagac caggaaatca gaaaaagggc
     4201 tgctgaatgc ggcaagaccg tttccggttt tttacgggcg gcagctctcg gtaagaaagt
     4261 taactcactg actgatgatc gggtactgaa agaagttatg agactggggg cgttacagaa
     4321 aaaactcttt atcgacggca agcgtgtcgg ggacagggag tatgcggagg tgctgatcgc
     4381 tattacggag tatcaccgtg ccctgttatc caggcttatg gcagattagc ttcccggaga
     4441 gaaaactgtc gaaaactgac ggtatgaaca ccgtaagctc ccaaagtgat caccattcgc
     4501 tttcatgcat agctatgcag agagctgaaa acgatcctga cgcatccttc ctgtttttcc
     4561 ggggtaaaac atctcttttt gcggtgtctg cgtcagaatc gcgttcagcg cgtttcagtg
     4621 gtgcgtacaa ttaagggatt atggtaaatc cacttactgt ctgccctcgt agccatcgag
     4681 ataaaccgca gaaatcgtgt cagccagcag cctggatttt ttcggggtaa ggtctttgct
     4741 gcggtaatca gtacccgcaa attgagttgt gccccggagt gagtttaaaa attcctggct
     4801 gtgaaactgt tgaatgcgtt gttgcacatc actccgtgat tttcctgtcg ctttatcttt
     4861 agctcccata tcccagaact gccgaaaaaa aggcaccatc attcgtgttg tgtcggcttt
     4921 ttgggtggaa aagttgggat caaaaccgtc atcagaagca tatacgtaat gccgggtatg
     4981 ttgttccacg ctgccctttg gggtacaggc tgtgagagct actgttgctg ttatcatggc
     5041 gataagacaa agtttgtttc cggtacgtct catgagtgtc tccctacctt aaagtattta
     5101 ttctcggtga gttattatcc gaagattatc ttatcatcag tttctgagcg cgt
//
