LOCUS       CP077386                4407 bp    DNA     linear   BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1387 plasmid unnamed7.
ACCESSION   CP077386
VERSION     CP077386.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357529
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1123.33x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:32:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,017
            Genes (coding)                    :: 4,772
            CDSs (with protein)               :: 4,772
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 80 of 245
            Pseudo Genes (incomplete)         :: 148 of 245
            Pseudo Genes (internal stop)      :: 51 of 245
            Pseudo Genes (multiple problems)  :: 30 of 245
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1387"
                     /culture_collection="FDA:FDAARGOS 1387"
                     /db_xref="taxon:562"
                     /plasmid="unnamed7"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            <1..496
                     /locus_tag="I6L17_25655"
     CDS             <1..496
                     /locus_tag="I6L17_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QXB84690.1"
                     /translation="AEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNETKIRDAIA
                     LFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASGGTLWYLGG
                     RIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQGKEWVHLK
                     E"
                     /name="MbeB family mobilization protein"
     gene            503..736
                     /locus_tag="I6L17_25660"
     CDS             503..736
                     /locus_tag="I6L17_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QXB84691.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1102..2241)
                     /locus_tag="I6L17_25665"
     CDS             complement(1102..2241)
                     /locus_tag="I6L17_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="QXB84692.1"
                     /translation="MTDNIAATIKGKRERLHMTQKEFADALGLSKYGDRTIRRWERGET
                     KPTGAELKAVMDFPDTPPYPNNENGRYRMIDLFAGIGGTRLGFHQTNAVNVVFSSEWDK
                     FAQKTYHANYGDFPDGDITKIDEKDIPDHEILVGGFPCVAFSQAGLKKGFNDTRGTLFF
                     DIARIIKEKKPHAFLLENVKNLLGHDKGRTFSIIKNTLEELNYTVYYNIFAAKDFGVPQ
                     NRERIYIVGFNKEKVRNHEHFTFPTPLKTKTRVGDILEKSVDNKYTLSDALWNGHQRRK
                     LVNAAAGKGFGYGLFNENSPYTNTISARYYKDGSEILIEQKGSNPRKITPREASRLQGF
                     PSDFIIPVSDTQAYKQFGNSVAVPVINAIAEKIISTLDS"
                     /name="DNA (cytosine-5-)-methyltransferase"
     gene            2351..3268
                     /locus_tag="I6L17_25670"
     CDS             2351..3268
                     /locus_tag="I6L17_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="QXB84693.1"
                     /translation="MQRLSPGEFKTLISKERKSHFITPFALVYKTFCDLGYDQKNSDYF
                     LNNPSEYIIAMRKNCWKEFEPFEKEFTTRMLSYLIDEERIKDMSPYDAIRDFTMEYPTH
                     IYDLALSNTQSRRSRAGKEFESILELLMMGAGIPVDVQGAIGKSFFQKNQIGKLVDLVM
                     PGVVQYTSNKRNTMLISAKTTLRERWQEVPEEVNRTGIREMYLATLDDSFSEETINILY
                     EANVVVVTTIENKNFKYKNNNRVLTFEDMLQSAMELSRKWNNVSYTDSEKEEIQQSILK
                     QIEKYSDFPYVVNYYRNRLSALFD"
                     /name="restriction endonuclease"
     gene            complement(4291..>4407)
                     /locus_tag="I6L17_25675"
     CDS             complement(4291..>4407)
                     /locus_tag="I6L17_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QXB84694.1"
                     /translation="LNELDADEQADICESLHDHADELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          4119..4206
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(3550..4095)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.171"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgcggagttc gagaaaaagc tgaaagagag agccgaatct acagagaaaa tgctgaacga
       61 cgagttcagg aagctcggcg agtctgtcag cgaggctgtg acctcaaacg agacgaaaat
      121 cagagacgct atagccctgt tcacagcctc gacagagaaa tcgctggaaa aacaccggga
      181 aggggtgaaa gaggcgatga tgcagcacag gaaggacgtg ttaaagctgg cagggaatac
      241 gggcatgatg ttactgggga tagtctttct cctgtttacc gcgagtggcg ggacgctctg
      301 gtatcttggc ggacggatac agacgaacct ggaagaaatc aggaagcagg aagagacatt
      361 gcagaaactg aacgcgaaga catggggcgt ggagtttgtg caggacggga acaggaaatt
      421 ccttgtcctt ccgtacggga aatcagcgga ggtgattccc tttcagggga aagagtgggt
      481 acatctgaag gagtgacaca gagtgacaga gctggaaata catttgctga acgccttaga
      541 gcagctgcaa caggactata tgcagcggct gagcgaatgg gagagcgcct tcgtggaatt
      601 gcagaagatg ttttcgctta cgcaacggga caacgcgatg ctgaacgagc gggtcatgca
      661 gttgagtcag caggtgcagc acttgagcga gcagacagaa cgcttgagcc agttatacag
      721 cgagaactgg agataagaga ggaacggctg atacaggagc gcgaacatgc gttatccctg
      781 gaacgggagc gtcagccgga aatacaggaa cgcacgctgg atggtccttc gctgggatgg
      841 tgaaaccatg aaaaatggca gcttcagtgg attaagaggg gttaatgtgg cctgtaccat
      901 ctggttgcat aggtattcat gcggttaaaa tttatcaggc gcgatcgcgg cagtttttcg
      961 ggtggtttgt tgccattttt acctgtctgc tgccgtgatc gcgctgaacg cgttttagcg
     1021 gtgcgtgtaa tgcgacgtta tggtaaatcc atatgggggg gtaattggat tctgggccgc
     1081 attgtgtcct tatgacttag tttatgaatc taaggtcgat atgatttttt ctgctattgc
     1141 attgataaca gggactgcga ctgaattccc aaattgctta taagcctgtg tatccgatac
     1201 agggataata aaatcactag gaaacccttg aagtcttgac gcttcccgcg gagttatttt
     1261 tcttgggtta cttccttttt gctctattaa tatttcacta ccatctttat agtacctcgc
     1321 acttatggta ttagtatatg ggctattttc attaaataaa ccatacccaa agcctttacc
     1381 ggctgctgca tttacaagtt tcctgcgttg gtgtccattc cagagcgcat ctgatagcgt
     1441 atatttattg tctacgctct tttcaagaat atctccaact cttgtttttg tttttaacgg
     1501 ggttggaaaa gtaaaatgtt catgatttct tactttttct ttattaaagc ctacaatgta
     1561 tatgcgttct ctattttgtg gaacaccaaa gtcttttgca gcgaagatat tgtaatacac
     1621 agtgtaattc aattcttcaa gtgtgttttt tattattgaa aaagttctac ctttatcatg
     1681 tcctagcaag ttttttacat tttcaagtaa aaaagcatga ggtttttttt cctttattat
     1741 ccttgctata tcaaaaaaca aagttcctct tgtatcatta aatccttttt tgagccctgc
     1801 ttgagagaat gctacgcaag gaaacccacc gacaagaatt tcatgatcag gaatatcttt
     1861 ttcatcgatt tttgttatgt ccccatcagg gaaatcacca taatttgcat gatatgtctt
     1921 ttgagcaaac ttatcccatt cgctggaaaa aacaacattt acagcgttag tttgatgaaa
     1981 tccaagtctg gttccaccga ttccagcaaa taaatcgatc atgcgatatc tgccattctc
     2041 gttgtttggg taaggtggtg tgtctggaaa gtccataact gcttttagtt ccgccccagt
     2101 aggttttgtt tccccgcgct cccatcttct tatggttctg tctccatatt ttgaaagacc
     2161 aagggcgtct gcaaactctt tttgagtcat atgaagtctt tctctttttc ctttgattgt
     2221 tgctgcgata ttatcagtca tgcatgtcta ccagaattaa gtgttggttt taggacaatt
     2281 tgtcctgttt tgattccaat tatgaacgtg catgaaaaaa ctttcaagta gatataaaag
     2341 gttatttttt atgcaaagat tgagtcctgg tgaatttaaa actctaattt ctaaagagag
     2401 aaagtcgcat tttattacgc catttgcttt ggtttataaa acattttgtg atttagggta
     2461 tgatcaaaaa aatagtgatt attttttgaa taatccaagt gaatatataa ttgcaatgag
     2521 aaaaaactgt tggaaagaat ttgaaccatt tgaaaaagaa ttcacaacaa gaatgttatc
     2581 atatcttatt gatgaagaaa gaataaaaga tatgagtcct tatgatgcca ttcgtgattt
     2641 tacgatggaa taccctacac acatatatga tttggcactg tctaacactc aatccagacg
     2701 tagcagggca ggaaaagagt ttgagtctat tcttgagcta ttaatgatgg gggcaggaat
     2761 tcctgttgat gtgcaggggg ctattgggaa gtcatttttt caaaaaaacc aaataggtaa
     2821 acttgtagat cttgttatgc caggtgtagt ccagtacaca tctaacaaaa gaaatacaat
     2881 gttgatttcc gctaaaacaa cgctgaggga gcgatggcaa gaagtacctg aagaggttaa
     2941 tagaacagga attagagaaa tgtatctagc tacactagat gattctttta gcgaggagac
     3001 aataaatata ttgtacgagg ctaatgtggt cgttgtaacg acgatagaaa ataaaaattt
     3061 caaatataaa aataataata gggtgctaac ctttgaagat atgctacagt cagcaatgga
     3121 attatcaaga aagtggaata atgtaagtta cacagactct gaaaaagagg aaattcaaca
     3181 gtcaatatta aaacaaatag aaaaatatag tgatttccct tatgttgtta attattatag
     3241 aaataggctt agtgctcttt ttgactaaaa aactaaaagg atctcttgag atcctttttt
     3301 tctgcgcgta atcttggacc tgtaaacgaa aaaaccaccc tggcaggtgg ttttttcgaa
     3361 ggttagctaa tcctggcaga ttatctaacc gaggtaatct ggcttcagca gagcacagat
     3421 accaaatact gtccttccag tgtagccgta gttaggccat cacttcaaga actctgtaag
     3481 catctggata aatctcgctc tgctaatccg gttaccagtg gctgctgcca gtggcgttaa
     3541 ggcgtgtctt actgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3601 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgggccgag
     3661 ataccaacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3721 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3781 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3841 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcggcgcgg ccttttgttg
     3901 ccctttgcct ttttactcac atgttctttc cggccttatc ccctgattct gtggataacc
     3961 gtatttccgc ctttgagtga gccggcaccg cttgccgcag ccgaacgacc gagcgcagcg
     4021 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta tgctctcctt acgcatctgt
     4081 gcggcatttc acacccggca tggcgtgctt ttcatacaat ccgcactgat gccgcatagt
     4141 taagccagta tacactccgc tatcgctacg tgactgggtc agggctgcgc cccgacaccc
     4201 gctaaaaccc gctgacgcgc cctgatgggc ttgtctgctc ccggcatccg ctcacagaca
     4261 agctgtgacc gtctccggga gcagcatgtg tcagaggttt tcaccgtcat ccccgaaacg
     4321 tgcgaggcag ctgcggtaaa gctcatcggc gtggtcgtga agcgattcac agatgtctgc
     4381 ctgttcatcc gcgtccagtt cgttgag
//
