LOCUS       CP077385                4072 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1387 plasmid unnamed6, complete
            sequence.
ACCESSION   CP077385
VERSION     CP077385.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357529
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1123.33x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:32:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,017
            Genes (coding)                    :: 4,772
            CDSs (with protein)               :: 4,772
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 80 of 245
            Pseudo Genes (incomplete)         :: 148 of 245
            Pseudo Genes (internal stop)      :: 51 of 245
            Pseudo Genes (multiple problems)  :: 30 of 245
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1387"
                     /culture_collection="FDA:FDAARGOS 1387"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            join(3710..4072,1..1797)
                     /locus_tag="I6L17_25640"
     CDS             join(3710..4072,1..1797)
                     /locus_tag="I6L17_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QXB84689.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSLMPDERTGLIHDYTRK
                     SGVAEAVILTPATAPTWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLHDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFETQWQQWGASGLAALRAQL
                     LKREEELRRKEQEDTERRRAERRAEQLRQNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     SYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQHTRNLRQPKTWPEFFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNSTLYPAFAVPSADNRHLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            complement(2044..2745)
                     /locus_tag="I6L17_25645"
     CDS             complement(2044..2745)
                     /locus_tag="I6L17_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QXB84687.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3278..3532)
                     /locus_tag="I6L17_25650"
     CDS             complement(3278..3532)
                     /locus_tag="I6L17_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QXB84688.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobilization protein"
     Typing          complement(3534..3709)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.067"
                     /coverage="100.0"
                     /evalue="9.53e-76"
     Typing          3710..4068
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.444"
                     /coverage="100.0"
                     /evalue="1.88e-155"
     Typing          complement(2045..2745)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.858"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..1794
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.993"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtggcgttcc atcacatgga taaaacaaat ccccatgcgc acatcatgct gaccacgagg
       61 gctgtcggac ctgctgggtt cggcggaaaa gtcagggact ggaacgaccg gacacatgcc
      121 gagacgtggc gggcatcatg ggctgaccat gcaaacaggg cacttgcgaa cgccggttat
      181 caggaagaga tagaccatcg ttcatacgag cgacaggggc ttgagaaaac gcctggtatt
      241 cacctcggaa agtctgcctg tgcgatggaa acacgcggta tcgaaacaga acgcggcgaa
      301 cagaatcgtc tgattaacag gcttaacctg gaaatacaga tttcccgcac cgaactcagg
      361 aacagcgggc tgacagagcc tgcccgtcgt gtggctgatc ttctgaatat cgtgcttcct
      421 gacaacgcaa cttcgtacac actgcatgac atcatcgaag cgttgccgaa ggacagtaac
      481 gcggcatgga aactcaccag taatgccatg tcgatgatgg cagatatgca ggcgacgcgc
      541 agacgctggg aagagctgaa caccgaacgg aaggaagcta tgagccatgc tgccagcctg
      601 aaaaaatcat cgccgttttc gtccggtttt tcccgtattc ctctgatgca atgggcggca
      661 ccggaatacc gcagtgagca gggaaaaatc cagtctctga gccagcaaat ggaaaaattg
      721 cgacagcatt accggaaggt ggagaaacag gacattcccg cgagtcagac ggcatttgaa
      781 acacagtggc agcagtgggg ggcatccggc cttgcagctc tcagagccca gttgcttaaa
      841 cgtgaagagg aattgcgccg gaaggagcag gaggacacag aacgtcgtcg cgcggagcga
      901 cgggctgagc agctcaggca aaatgacaat gccgtcatgg attcaggggt actgagtgcg
      961 gtcgtaacgg ggtatggcga ggcccccatg cctggtaacg gggaaatgac cagttacctt
     1021 ctgcttcaca accggaacgg agaatatacg ctctggggga acgaactgga gaaataccgc
     1081 accagaatct ttgagcgtgt tgatttaatg cgtgacagga gtggttatat ctgtgaccga
     1141 agcgaaatcg gtcaacatcc accactgcaa cgtgtttatt cctctgcgac ctttgagcaa
     1201 ctgctgacgc aggtttgcca gacatggcca caacataccc gcaatctccg ccagccgaag
     1261 acatggccgg agtttttctg ccttggagaa gaccgccagc cagccatgcc atcactggca
     1321 gcaaggaagg tggattttac gcagggacgt ctgccaccga cgctgatgcc ggtaatgagt
     1381 tctgtcgacc gggaaacccg ccagcttcag cttttgctgg tcatgggcgt ggatgactct
     1441 ctgggcggtg ttgtccggct taatagtacg ctgtatcccg cgtttgccgt tccctcagcc
     1501 gataacaggc atctagtcat cagtgcgctg accgataagg ggttacggta tgcagggtat
     1561 ggtgttgccg tcaatcacga tgcagacagc cacatatcac ctgcaccgga acttatggag
     1621 tttcatctga aaacccgtga ggcaccgctt tttgctgccg tcaacacacc agaaaaacag
     1681 ccggatcatc tgttccgctc gctgggtttt aatcgtacct gggacgaatg gcggcgcgag
     1741 gaagatgcac gaacacacgc caccgagagg aggcacgatc gcggttggtc acagtgaatg
     1801 tgaacaatat tttgttttgt tttacatcat gttatgttct tcaatgttca tcgacaggga
     1861 tgaatgcaag aagcacacac aacaaactag ataatctccg catagtttct tccccccgta
     1921 tatgggggat ttttttagct cgcgagagcg attctgacga tgtttttgtg cttcgtagca
     1981 ccatcgcata ccttacgcaa aaaaaacgca ccagagcgcg ttttagatgc cttaaaatcg
     2041 attttagatc gtcatcttga tttgtttgtc ttccttgaag cgaaaatgca gggctacaac
     2101 cgtgcgcccc tttttgatcg tctcaaccgt tacagccagg tcgctttttt tgttcagttc
     2161 gtcaatgcag ggtcttaata attgctggtt aagtgattta tatgtttgat atttatgctc
     2221 tatatcaagc attgagcgaa aatcatcgag agcgataata cgttcgccag tactgcggaa
     2281 ctgctggagg agttcgtata tccttatact gtaacttctt gataatctag atatattctt
     2341 gattacaact ctagtaaatt gcccttgtag ttgtgtcaaa taaggcatta ctgcgtcaga
     2401 aaatgttatt tgtgctctgg cctcgccttt agcgtactgt gcccgatact ggatccagcg
     2461 gaactcctca cgtttctcgt catccttgag aatgattgac ctgtcccaca aacgatcaat
     2521 tgcttcctgt aactgaacct cggcattttt ccgccccatg tcagggaatg aatcgaaata
     2581 ttccgcagct gtaatggtca tggtcttttg caattttgga gattctgcat cagccccaga
     2641 cttcaatcga ccgatgcaaa gtaacaaaaa tcgctgttcc tgtagggtca atttataact
     2701 ggcttcaacc agcgcattgg atttataggc aattagatcc gacatagagc atccctcccc
     2761 aaaaggtaat gaataaaaga taaacatacc ctcagcgaaa ggcaatgcct ttttatttta
     2821 atatcccttt ttctgtgaat attaccttaa atcatattgc ctttacctta aatcatatta
     2881 cctaaacacc ttaaatcata ttacctttac cttaaatcat attacctata gcgttgtaac
     2941 attttgtttt ctataaggaa ttttggcact aaaacaagta ttaaaaacaa gatcttaaaa
     3001 acaagatcta aaaacttaac gacgagcctg tggataactt ggagtgctga aaacaaaaag
     3061 ccaataaaaa caatgcgttg caaaaaagcg gaattgcaag ttccgaatac accccctcca
     3121 accctgtgga aatccagtat tcatgcgggt tcagcgcata tcacctgccg aaaaaccgtg
     3181 cgtatgtgct caatcaggtg acggattcac ttccgcttcc tcgcccactc gctcgctaac
     3241 gctcggtcgc tggggctgcg gcgagcggac gatgcaatca ctgttctggc agcgcctcca
     3301 gctcgttaat cagcggcagt attcgcgtca catcagcctg ccgtgtcacc tctgccttca
     3361 ggaactggag taaccaggcg cgcaattgca cctcctccgg ctttctgacc tttgccagca
     3421 atgcagcacc tacaacgacc ttctgtgctg tgtccaggct ccggctcttt gccttcagac
     3481 gctgcaatct ggcttcagct tcagcaattt tctgttcgag tgttctgctc atgatgtaac
     3541 tccgtgcgcg gtgaaaaatt gcattttagc tcgtcactgg tagtttaaaa actgagctgg
     3601 cataatgcgc agaaatcacg aagtgcgcac ttatacaatc ttcactccgt ttcgattgtg
     3661 tgcgccctgc ggggctgaaa gaaaactgca aaaaggcatt acggcagaaa tggcgattta
     3721 tcatctcagc atgaaaatca tttcgcgaaa aaacggctac agcgccgttg cttctgctgc
     3781 ctatcgttcc ggttcactga tgcctgatga gcgtaccgga ctgattcacg attacacccg
     3841 caaaagcggc gtggctgagg ccgtgattct gactcccgca accgccccca cctggtgtac
     3901 gaaccgtgcc gaactctgga atgctgttga gaaggctgag cgccggaaga actcacaact
     3961 tgcgcgggaa attgaactcg ccatcccccg cgaactgccg caggatgccg cacgggaaac
     4021 cgttctcgcc tttgtcaggg aaaactttgt cagtcagggc atgattgctg at
//
