LOCUS       CP077384                1822 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1387 plasmid unnamed5, complete
            sequence.
ACCESSION   CP077384
VERSION     CP077384.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357529
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1123.33x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:32:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,017
            Genes (coding)                    :: 4,772
            CDSs (with protein)               :: 4,772
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 80 of 245
            Pseudo Genes (incomplete)         :: 148 of 245
            Pseudo Genes (internal stop)      :: 51 of 245
            Pseudo Genes (multiple problems)  :: 30 of 245
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1387"
                     /culture_collection="FDA:FDAARGOS 1387"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            join(1654..1822,1..365)
                     /locus_tag="I6L17_25630"
     CDS             join(1654..1822,1..365)
                     /locus_tag="I6L17_25630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="firmicute plasmid replication protein RepL"
                     /protein_id="QXB84685.1"
                     /translation="MLEQQFEDFEQQKRRDHQAQDQSKSAKQKRDRLNPPDWSQVTKAG
                     WNMLDSISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDLES
                     IGAIVKIPVGIYYAYALDPYQVWRGYNKSKKYAAFNAKTLTQKDGVIDRKLRLMLSRRE
                     EFEGSLKTSNHDLD"
                     /name="firmicute plasmid replication protein RepL"
     gene            378..665
                     /locus_tag="I6L17_25635"
     CDS             378..665
                     /locus_tag="I6L17_25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXB84686.1"
                     /translation="MRIKYESRWKLEELKLEICRSIDSLIDEYHIEYAMGINLYMNCED
                     KEFNPVDIVNEDGKNIMFVVSNNEKIQKPKKSAKKKIPAPVIELDKYRKK"
                     /name="hypothetical protein"
     Typing          complement(908..1168)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.985"
                     /coverage="100.0"
                     /evalue="1.85e-99"
ORIGIN
        1 ccagagcatt taggctatat tctttttttg ctcaaaatat agataaatcg tgtggggctg
       61 ttgtatgtga ccaaaaattc ttagctgatt atttcggtgt tagtttaagg acgatccaat
      121 attggattgg agatttagaa agtattggtg ccatcgtgaa aataccagta ggtatctatt
      181 atgcttatgc gttagatccg tatcaggtat ggcgcggata taataaatct aaaaaatatg
      241 cagcgtttaa cgcaaaaaca ttaactcaaa aagatggcgt tattgatcgc aagttgcggc
      301 tcatgctctc taggagagaa gagtttgaag gctcgcttaa gacatctaat catgacttgg
      361 actaggtatg atgcgctatg agaattaaat atgaatcacg ttggaaatta gaagaactaa
      421 aattagaaat atgtagatca atagattcgt taattgatga atatcatatc gaatatgcaa
      481 tgggaataaa tctatatatg aattgtgaag ataaggagtt taatcccgtt gatattgtca
      541 atgaagatgg aaagaatatt atgtttgtcg ttagcaacaa tgaaaaaata caaaaaccaa
      601 aaaaaagtgc gaagaaaaaa attcctgcac ctgtaattga gttagataaa tatcggaaaa
      661 agtgatggcc atacagagcc agcggcaggc ggacaatcca cagaacattg catgcaattt
      721 ctagttgatt gtctatcctg cattgctctt ttgccgcctc aaaatccctt gcgtcttttt
      781 gctccccgtt ttccagaacg accgagccgc cacggttccg gcagcgcctt gagcgcaacc
      841 ggaaaccgtc tggggagaac cccagacccc catcgctgta acatcgacgg tctacgacca
      901 cctaccaacc gcgtggctgc ctattccgtg caaatgggct ttgcaggggg ctgacgcggt
      961 tcctggcgac ctgaaatccc ctggttaggg gtatcggcgc gcctgacctc ctaaaaccct
     1021 ctgtaagcgt ttttcgcgcc ttccatgccc tgatgggcat ttaaaacccg tttcgtgcgt
     1081 ctgagagctt ctgagagcgt ttgaagagct atctggcgac tcatccccga aagaacaaca
     1141 gcggaatcac cgataacatg cgagaggtgg caccgctcgc cgcagcccca gcgaccgaac
     1201 gttagtgagc gagtgggcga ggaagcggaa gagaaccgga aaccacattg agcacgtacg
     1261 cactgatgcg gggcgtttta gcggtgaacg gactgcaaaa acatgatcga gctgttgaac
     1321 gtggagagcg cgaaatcggg caaaatcacg gctgacgagg cttgcagaag agtaagccag
     1381 tatacactcc gctatcgcta cgtgactggg tcagggcttc gccccgacac cccaaatgtt
     1441 cgatgaacta attaaatctt gtgtagcaaa aaagtgcttt gatagatctt tagagcaaaa
     1501 atcgacattc tcttaaaaat caaagcatta tgacaccaac atacgaaacg ttatttgcat
     1561 gtgtatacgc aaacatgttt gcgtgtgttg tttcgtaatg tacgatataa atttcgtaag
     1621 gtacgaaaca acgttgcagg taaggatgca agtatgctgg aacagcaatt tgaagatttc
     1681 gaacagcaaa aacggcgaga tcatcaggca caagatcaat ccaaaagtgc taagcaaaaa
     1741 cgagatcggt tgaatccacc tgactggtcg caggttacta aggcgggctg gaatatgttg
     1801 gactctatat ctgagaaggt tc
//
