LOCUS       CP077383                2101 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1387 plasmid unnamed4, complete
            sequence.
ACCESSION   CP077383
VERSION     CP077383.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357529
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1123.33x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:32:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,017
            Genes (coding)                    :: 4,772
            CDSs (with protein)               :: 4,772
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 80 of 245
            Pseudo Genes (incomplete)         :: 148 of 245
            Pseudo Genes (internal stop)      :: 51 of 245
            Pseudo Genes (multiple problems)  :: 30 of 245
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1387"
                     /culture_collection="FDA:FDAARGOS 1387"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            complement(join(1861..2101,1..725))
                     /locus_tag="I6L17_25620"
     CDS             complement(join(1861..2101,1..725))
                     /locus_tag="I6L17_25620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QXB84684.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            complement(1591..1857)
                     /locus_tag="I6L17_25625"
     CDS             complement(1591..1857)
                     /locus_tag="I6L17_25625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXB84683.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          complement(101..332)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
       61 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      121 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      181 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      241 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      301 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      361 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      421 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      481 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      541 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      601 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      661 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
      721 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
      781 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
      841 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
      901 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
      961 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1021 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggccta ccccaacctt
     1081 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1141 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1201 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1261 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1321 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1381 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1441 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1501 gctgaggata atgcaggata gacagagcac tagagattgc atgcaatctc gtacctatca
     1561 cctcagcctg ccgctggctc ttttccagaa ctaagaatgc gttcttaaaa atccaaactt
     1621 gataccgttt aaatctattt tcacaaaaag agtttttacc ctctcgtttg ttttctgaat
     1681 atataaaatt aattcatcct caaaaccttc gtgcggatga aaaccaaatg tgccaaaact
     1741 taatccatca ataagcaaaa taaggttttt aaacctgtca ggaatcaccg cttcacttct
     1801 taaaaaaaat aaaccaatag tcttatttcc attcaggtat tcaatattaa tattcatacg
     1861 ttaatccgat ttatccttct caggggcgcg tttgtaacgc cgtttacctg aatcccagac
     1921 aaacgccgtc cgcttcccgt catcagtgcc atcccctaca tcgtcggcaa tgacaagatc
     1981 ctcattggtt tctcgatcca actggaggac gttttttagc gccccaccgg tcgagataaa
     2041 acggcgcttg tgaagctgcc gcgtcagctc aagaaaccac tcaggatcgt ttgccatatc
     2101 t
//
