LOCUS       CP077382                5631 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1387 plasmid unnamed3, complete
            sequence.
ACCESSION   CP077382
VERSION     CP077382.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357529
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1123.33x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:32:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,017
            Genes (coding)                    :: 4,772
            CDSs (with protein)               :: 4,772
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 80 of 245
            Pseudo Genes (incomplete)         :: 148 of 245
            Pseudo Genes (internal stop)      :: 51 of 245
            Pseudo Genes (multiple problems)  :: 30 of 245
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1387"
                     /culture_collection="FDA:FDAARGOS 1387"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            join(5060..5631,1..295)
                     /locus_tag="I6L17_25585"
     CDS             join(5060..5631,1..295)
                     /locus_tag="I6L17_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="QXB84679.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(1498..1689)
                     /locus_tag="I6L17_25590"
     CDS             complement(1498..1689)
                     /locus_tag="I6L17_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QXB84680.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1754..2077
                     /locus_tag="I6L17_25595"
     CDS             1754..2077
                     /locus_tag="I6L17_25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QXB84682.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2019..2777
                     /locus_tag="I6L17_25600"
                     /pseudo=""
     CDS             2019..2777
                     /locus_tag="I6L17_25600"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2755..3285
                     /locus_tag="I6L17_25605"
     CDS             2755..3285
                     /locus_tag="I6L17_25605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QXB84681.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            3292..3525
                     /locus_tag="I6L17_25610"
     CDS             3292..3525
                     /locus_tag="I6L17_25610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QXB84677.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4006..5058
                     /locus_tag="I6L17_25615"
     CDS             4006..5058
                     /locus_tag="I6L17_25615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXB84678.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     Typing          1323..1404
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="100.0"
                     /evalue="6.23e-34"
     Typing          679..866
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          2068..3629
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtcatcgctc tttattttta tatctaaatc atcaatttat cttccgttgg aatccgtgat
       61 tattgttttt tcatctggaa tattacaata ttttttagct ttttgttttt acttgaactc
      121 cttgcgttgg ctcccagttc atttggcagg gataatgttg tactttattc cgctagttgc
      181 attatttttg tcatggttgt ttttggatga acaattcaca ttcttacagg gggtaggatt
      241 tattgtaact gtgctaagtg tatattttct taacagaaaa tactcagagg agtaacattc
      301 tgttcaggat tgccccccgg cctccatcga taccgtgcca aatgcacgta aaaaacggga
      361 gcgtttctgg tgtcggaatg taaccaaggg ggtcagaggc gcgctaaaac gctctcaggg
      421 gagtcaaata gagacgtttg taacaaaaag atcaaaggat cctcttgaga tccttttttt
      481 ctgcgcgtaa tctgctgcct gtaaacgaaa aaaccgcctt gcagggcggt ttgatcgaag
      541 gttaagtcag cttgcagact gcttaaccag gtaactgtct tatgcgagac gagtcaccaa
      601 aattgttctt ctagtgtagc cgcactccgg cagccacttc aagtactctc taaatatctc
      661 gcacatgact tttaccaatg gctgctgcca gtggcgcttt gtggtgtctt ttccgggttg
      721 gacccaagag aacagttacc ggagaaggcg cagcagtcgg gctgaacggg gggttcctgc
      781 atacagccca gcccggagcg aactgcctaa gtcgccccga gataccagaa gacgtgagct
      841 atgagaaagc gccacgtgtc ccgtaaggga gaaaggcgga aaaggtatcc ggtaatcgat
      901 atggcgggaa cgtgggggcg caggagggag cgattcaccg gaaacggtga tgatctttaa
      961 gccacgtcgg gtttcgccac cgctggcgga tttcatggtt gagccgcaag gctcccacag
     1021 atgcaccgaa aaagtgtctg ttttgtgaag tccgtcaggg gggcggagcc tatggaaaaa
     1081 gcgcctccgg cgcggccttt cccggggttt cgttttttgc gcacatgttc tttcctggtt
     1141 tatccccggg ttctgtggat aacagggtta gcgtttttga gaaaggagag tccgctcgcc
     1201 gcagccgaac gaccgagcgt agcgagtcag tgagcgagga agcggaagag cgcctgaact
     1261 ggcattttct ccttacgcat ctgtgcggca tttcacaccg gacatggcgc acttttcata
     1321 caatccgcac tgatgccgaa tggttaagcc agtatacact ccgctatcgc tacgtgactg
     1381 ggtcagggct gcgccccgac accccaaagg tcaacggcct gacgcgccct ggcgggcttg
     1441 tctgctcccg gcatccgctc acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     1501 gcggttttca ccgtcatccc cgaagcgtgc gaggcaactg cggtaaagct catcggcgtg
     1561 gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagttcgt tgagcttctc
     1621 cagcagcgtt aatgtctggc ctctgatgaa gcgggccatg ttaagggcgg ttttttcctg
     1681 tttggtcact gatgcctccg tgtaaggggg ggttctgttc atgggggtaa tgataccgat
     1741 gaaacgcgag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga
     1801 acgctgtgag ggtaaacaac tggcggtatg gatgcggcgg gtctgcctgg gtgagccggt
     1861 tgcccgttcc gggaagctgc cgacactggc accgccgtta ctgcgtcagc tggccgccat
     1921 cgggaataac cttaaccaga cagcgaggaa ggtgaacagc gggcagtggt cttccggtga
     1981 ccgggttcag gtggtggccg cactgatggc catcggggat gagctgcgcc ggctgcgtct
     2041 ggcggtcagg gaacaggggg cgcgggatga tagttaagtt tcatgccagg ggaaaaggtg
     2101 gtggcagtgg tccggttgat tacctgctgg gaagggagcg taaccgggaa ggggcaacgg
     2161 tgcttcaggg gaatccggaa gaggtccggg aactcatcga tgccacgcca tttgcgaaga
     2221 aatacacgtc tggtgtgctg tcgttcgcgg agaaggagct gccaccggga ggacgtgaaa
     2281 aagtgatggc gagctttgag cgtgtactga tgcccggtct cgaaaaaaat cagtacagca
     2341 tcctgtgggt ggagcaccag gacaagggac ggcttgagct gaattttgtc attccgaaca
     2401 tggagctaca gaccggaaaa cgcctccagc cgtactacga ccgggcagac aggccgagaa
     2461 ttgatgcctg gcagacgctg gtgaatcacc attacggtct gcatgacccc aacgccccgg
     2521 agaaccgccg gacgctgacc cttcctgata acctgccgga aacgaaacag gcgcttgctg
     2581 agggcgtcac acgcgggata gacgcgcttt accatgccgg agagataaaa ggccgtcagg
     2641 atgtgattca ggcgctcaca gaggcggggc ttgaagtggt cagggtgacg cgaagcagta
     2701 tcagcattgc agacccgaac ggcgggagga atatcagact gaaaggagcg ttttatgagc
     2761 aatcttttac agacgggcgc ggagttcgag aaaaagctga aagagagagc cgaatctaca
     2821 gagaaaatgc tgaacgacga gttcaggaag ctcgaagaat ctgtaaacag ggaactgacc
     2881 tcaaacgaat ctttaatcag gaacgctatc aacgaccata caacagccct gaaagaactg
     2941 ctggagaggt atcagaaaac gaccgtcgat acgatggacg cgcactggaa aaccgtactg
     3001 aaaatgagcg tgaaacgctg gctgtggctg attatcgtct cagtactgat gtttgccacg
     3061 accgggagtc tgctgtggta ccagggcatg aagataaacg cgaatatgaa tattctccgc
     3121 gagcagaaag agagtctgga gaagctgaac gcgaagacat ggggcgtgcg ttatcacgag
     3181 gacagcaacg gacgttttct ggtgttgccg aaggggatga aagccgaaac gaactggacg
     3241 aaggacaacg gcaaactgaa cgcagtgaga ctggtgcagg agtgacagag catgacggag
     3301 ctggaaagac atttgctgaa cgcattagag caactgcaac aggactatat gcagcggctg
     3361 aacgaatggg agaacgcctt cgtggaattg cagaaaatgt tttcgcttac gcaacgggac
     3421 aacgcgatgc tgaacgggcg agtcatgcag ttgagtcagc aggtgcagca cttgagcgag
     3481 cggacagaac gcttgagcca gttatacagc gagaacagga gataagggat gaacggctga
     3541 tgcaggaacg cgaacatgag ttatctctgg agcgggagcg tcagccggaa atccaggagc
     3601 ggacactgga tggcccttcg ctgggatggt gaaaccatga aaaatgacgt gttcagtgga
     3661 ttaaggcggg ctaatgtggc ctgtagcatc tggttgcata ggtattcatg cggttaaaat
     3721 ttatcaggtg cgatcgcggc agtttttcgg gtggtttgtt gccattttta cctgtctgct
     3781 gccgtgatcg caccgaacgc gttttggcgg tgcgtacaat gcgacgttat ggtaaatgga
     3841 gcggattttc aggaaggagt gatactaaca actcgaacac tgtgcaaata atgatttttt
     3901 taaaatatcg gtaacgataa taaattattt cgttttacgc cggtaataaa tataatgtag
     3961 tttcagtata taaaaaaata tatgaggtgt cttgtgggag ctttcatgaa tcttcaacaa
     4021 cgtataaata aacttccaca attatcatcc agtttttcat ttggaaaaga tattgataat
     4081 attcattcat ttatctttaa cgaaacttct aaagataaaa ttgaagattt actaagaaaa
     4141 tgggtgtcag gcaatcagcc ctgtgtattt ggtaaattgg caagcaagaa aattaaaggg
     4201 cttgatttcc atttatctat tgtaaatagc ccccaactat ataatgatga tggtcatttg
     4261 tttgattttt tgcgtaatga aagagttcgt tttaaagaac gcgcaagaag aggagaggtc
     4321 agtgcgcatc tcatttattt tattcatcca caacttgcct ttgctcgtcc atcagaagag
     4381 ttagtggaca tacaaaaata tatttgttcg ctgcatatgc cagagtgcta tccaataaaa
     4441 gaggatgtta tttatactga aagcgtacca tttcaggata aagatgggct aaaaatatat
     4501 aaggctggtg ttaatgtttt ttactcttct gctcatagaa caagaaatca tgatagaaga
     4561 ataccagggg gaatacttat ttctgtaaat gccccggggc attttatgag gctggcgata
     4621 gaaaaaggtt tctataaaga tcaagaacaa gcgttagcgg atattcgtaa catgactatt
     4681 cagtctgttg gtaagggtgg gtattcacat ccggaaggga tttctacaac atggcattca
     4741 gaatctaatt tggatcgttt tggatgccca gtgcacaccg gtaatagttc ttattacagc
     4801 ggtttttatc atactgatgt attaattcca ggagaattaa ctaaggatga aagattgctc
     4861 catgaaatag aaaatagtga tccaatgatc ttcaactgga atgtattgtt ttatgtttca
     4921 ttagaagagt ttcctataga tgatccttat tatggagagt ttattggtat tcctgtagat
     4981 gatgcatcta tgttttttaa ctcctttcaa ccaaggaaat ttgaaaacaa acccctttat
     5041 gagaaagagg atgattaata tgattaaagg aatgcttttt gcttttttag cagcgttctc
     5101 atggggggca gctatcgtaa tgagcaaaaa aggagttgaa gaactagatg ccggaggggt
     5161 gtttttctgg cagatttgta gtgcagtaat actatcttgg atagttcttt ttataaaaag
     5221 aaaaaaactc ccattaaact caaaagcgat acttgcttat gcgacaggcg tttttgaacc
     5281 attcttagcg tatactttta cactctacgg actttatttt atttcagcag gaagcgcatc
     5341 aattattttt tcacttgaat cagtgtttat attggtgttg tctgttctta tttgctcagt
     5401 taaaattaat tccccgcaat attttatatt gttccttata ggtgcaattg ttggcagtat
     5461 tttggtagcc ttgccagaca tcagtaatgg atcgaataat attgttggat attgtcttgt
     5521 tgttgccggt gtgttatcgg cagcatttta tgttgttata tcgtctcgcc ttattaaatc
     5581 gtttgagcct atcacattat tgagcggaca gttgacattc tcccttatac t
//
