LOCUS       CP077359                4775 bp    DNA     linear   BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1380 plasmid unnamed6.
ACCESSION   CP077359
VERSION     CP077359.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357522
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1152.65x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:44:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,077
            CDSs (total)                      :: 4,959
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 71 of 152
            Pseudo Genes (incomplete)         :: 88 of 152
            Pseudo Genes (internal stop)      :: 34 of 152
            Pseudo Genes (multiple problems)  :: 38 of 152
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1380"
                     /culture_collection="FDA:FDAARGOS 1380"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            96..494
                     /locus_tag="I6L10_25355"
     CDS             96..494
                     /locus_tag="I6L10_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="QXB65845.1"
                     /translation="MGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLP
                     VPLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVAT
                     SARTLKPWEALGISRAWYYQLKKRGLVE"
                     /name="primase alpha helix C-terminal domain-containing
                     protein"
     gene            complement(698..1096)
                     /locus_tag="I6L10_25360"
     CDS             complement(698..1096)
                     /locus_tag="I6L10_25360"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXB65841.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1273..2199
                     /locus_tag="I6L10_25365"
     CDS             1273..2199
                     /locus_tag="I6L10_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QXB65842.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSKQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2202..2735
                     /locus_tag="I6L10_25370"
     CDS             2202..2735
                     /locus_tag="I6L10_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXB65843.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(2801..3010)
                     /locus_tag="I6L10_25375"
     CDS             complement(2801..3010)
                     /locus_tag="I6L10_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXB65844.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          124..494
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1274..2196
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.727"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggagtggcg cgaggaagcc tataccctcg atgaactggc tgattatctc gatttgagcg
       61 cctcagagcg tcgtagcatc gataaacatt acgggatggg gcgaaactgc cacctgttcg
      121 aaatgacgcg caaatgggct tacagggcga ttcgtcaggg ctggccagcg ttctcacagt
      181 ggcttgaagc ggtgatccag cgtgtcgaaa tgtacaatgc atcgcttccc gttccgcttt
      241 cactggctga atgtcgggct attggcaaga gcattgcgaa atacacgcac aggaacttca
      301 cgccggaaac tttcgcacag tatgtggctg atacgcacac gccagaaatt caggctgcac
      361 gcggtcgcaa gggcggttct aagtctaagc gcagcacagt agctacatct gcacgcacgc
      421 tgaaaccgtg ggaggcatta gggatcagtc gcgcctggta ttaccaactg aaaaaacgag
      481 gtctcgtaga gtagaccaaa taagcctata tcagataaca gcgcctttct ggcgtctttt
      541 tgagcagcag gttttttgcc gtttttcccc gttcagccct gcaatggcgc ggctttccgc
      601 gtgattgtgg tggtagtgct cgccgcagtc tcatgaccga gcgtagcgag cgaatgagcg
      661 aggaagcacc agggaatcgg aacacggatg gaggcactta cgcgtctggg tttggggatg
      721 tctccagatc gtacttaagt tccttaagtg cctgtaatat cctgtctttt ttatagcata
      781 gctcgcctgt ttttgtgtct accagtcctg cttcgatcag tagcccagcc gcgagaaaga
      841 gccggtgatt gcgttcccgt cgttgttttt ttccctcgat tcgtttttgg accgcctgtt
      901 ttcgttcgct ctcctgtgcc tgtaagtcgt ttttttccgc tgttgccagt acgtggagaa
      961 gtttgcgttc ctgttctgtg agtccggctt cgttgtcgat caggtcaata atctgtctct
     1021 gtcgtgcggt ctgttctccg agcttcctga tgaatccgat ccgtttcttc agccattcga
     1081 tgttgctgat gcgcatgttc gtgatgctcc ctggtgtttt tttgcggtgt gttgtggtcg
     1141 tgcagtatat cgcttgcggt cggcgggtgc agggggtatc ttccgtgcta attcgtaaaa
     1201 ttagaaggaa gcgcacttat atgtttctct ccttcgtcga gaaacagtgc gctcctgcag
     1261 agggctgaaa ccgtggcaac ctatcacctc agcgttaaat ttggtggcaa ggggcaggct
     1321 gcaaaccacg ccgattacat cgagcgcaaa gagaaatacc gcgaccgtca ggatttggaa
     1381 tattccgcac acggaaacat gccggaatgg gccagggata atccttccca tttctggcag
     1441 gcggctgacc agtttgaacg tgcgaacggt tcgacctata gggaacttga aattgcatta
     1501 ccccgtgaac tgacaccgga acagcgcctt gaactggtgc aggattttgt acgtcaggaa
     1561 gctggcgaac ggcacgcctg gagttttgcc attcacaatc cgaaagcgag cattgacggc
     1621 ggcgaacagc ctcatgcgca tatcatgatg agtcagcgtg tgaatgatgg cattgaacgt
     1681 acaccggaac agtatttccg gcgctataac gccaggtatc ccgagcgggg aggtgcgaaa
     1741 aaggattccg gaagcctgac tctcactcag caaaaagagc agttgcggga attgcgaaaa
     1801 cgctgggagg tgaagcacaa cgaacatatg agaaagcatg gctttgaaag gggcttcatt
     1861 gactgccgga cgctgaaaga acagggcatt gaacgacgtc ccgaagtgca tctgggtttt
     1921 gaagctgccg gacggcttga tgatgcccag cgtcatgaca tcatgcaaaa acggtctgaa
     1981 ccggactatt cccgccatct tgaacaggcg cgaacggtcg ggcatgacat cagggaatgg
     2041 ctggacactc cggttaagca acatgaactg gtcagaaggc tgcaacatga tgttgcgcgg
     2101 gagcatggct ttgacatgag agacagagtg agcaaacaga accgaactgc acctgaacgg
     2161 gttaaatcac cgtcacgtgg attttctatg ggattctgaa catggccagc acagaagaaa
     2221 aattactcac gctcctgtta tcggtggagc atctgcaaca taccgcaatg gagcaacaga
     2281 aagctgttgc tgattctctg gctgtggcaa gggaaactct tcgcggatat cgggatattt
     2341 ctgacggtgt ggcaaaacag gtcagggatg aagtcagaaa tgaggtgcag aagctggacg
     2401 tggcaggact gataggtgag cgcatcagcc aggattttca gcggctggaa gagtcagtcg
     2461 caaacatgaa ggcaaacgcg gatgccctga acagggatat tgcgacaatt cacagcaata
     2521 tcgtgcagga atacaacacc cttaaagggt atagctggaa atggctgggt ggggtgtcag
     2581 tggcttttct ggttatgttc tgtgtgctcg gatggggaat gaaaacaata ctcgacagga
     2641 actacaacgc cacagcggcg gtgtatcaga aagttgtgac actggaagat aaactggtgg
     2701 agaaacctgt cacggggaag agccggaaaa actgacgcgc ttcgcttgtc caacgcctca
     2761 cgacccgcga aaggatttcg cgaaccgttc accgtttttt tcaatatgat tctgtctgct
     2821 ttggtcgata aagcagacga tagtattcca gccttcgtat atataacggt acgtgctcat
     2881 cagtaagagc tggcggtatt gtgttatcag ggggaagttt tttcagatac tcacgaaatg
     2941 cctggcttga tgccataaaa tctacagtag catcgataat atgtggggtg ttttcaccta
     3001 attttgccat gatgataatc cttatattat cacggtttgt gaatgatagg gtgtcactat
     3061 tatttatgcg ttagtgaacc agaaaaaatc ccccgttggc atagggggat atgtaaggag
     3121 aaataacttc gcaggtgttc ttgtttgtat aacgtgaaaa gactgcacgc aacttaataa
     3181 gtctgccgta cttactttca gttttcaaca taaggtttat aacaatgtat ggaatggaag
     3241 tgagctttaa ggtttcagct ctaaaaaata aaatatttat tagttggctg gttgttgttc
     3301 ttgttctgta tgtatataaa cactatcgac atattatgac cgggaacatt ccttaccccc
     3361 tctgcgctcg cagaggtttt ttttgtcatt aattaataat gaactgaaat gcagttccga
     3421 tacctccatt tgcacattat ccctgacata tggtaccgga accgcattaa ttttatgtta
     3481 taagagttta tttttaatgt ccagatggtt taatagtatt tatatgtaat tagctgtatg
     3541 gatagtactg gttttattgt ataacttgtc gtttttttta ttgtttttgt gtggtgtgct
     3601 aatctttaag ttataaaaaa accccttgga atacaggggc aaaagtaagg aaaacaaatg
     3661 gatgctcagt ttgtgtgaca ctttaaacta atggaactat agagtggagg ttgttacagt
     3721 cgcttttata aaacagcaac cagttttttt caatagtctg atgatggttg tgacagtttt
     3781 gtgatattgt tcaaaatgtt tcttgttgaa tgtttgtttt ttggtctatt ggtgtctggt
     3841 tttatttttt ctgctattac atatcaagaa atgtgattgg tgtagcaaga gtggaatgcg
     3901 atgtggataa actaattggt tgtcgaatct aatttactta gaaaactaca cttgtttttg
     3961 tgtatgaatt tagtgaattt gtctgtctat gtgttttcgt gcatttataa atccttcgtc
     4021 aggatcttaa atttagtgtt gaatatgtag ggcctgtacg gtaattatta tttagcgtag
     4081 ctacttcatc gttgttcgaa cacaaccaac aacctgtaat tgttgtttct cttggttccc
     4141 cacttttgtg gggtttttta tctcttccgt gcatcgtaaa gcgcggactc cttctggaaa
     4201 ggttgtttta gggcatcact gaggcatttt ttgcgtttgt gaatattgag catccgataa
     4261 ctaaaatatt tcccatagac tctgattctt gccaccgacc ccaggtcggt ttttttttgt
     4321 cctggcgaga tgtgaagttt ggaaacagga aaccccgtca gagcgccacg caccagggga
     4381 gaaaacttgt cgttttgagc cgttcggtgt tcagaacgca cgaaaccgat cgcacatcgc
     4441 tttcgtgcat agttatgcaa cgccctgaaa acgtttctga cgcgtttttc tggtttggcc
     4501 tggtgttttc ttgtcttttt gcgttttttg cgccagaaag cgattgaggg cgttttacag
     4561 ggtacgtaca acgggagtta tggtaaatgg atcggatttg cgggaaggct cgacaggttt
     4621 tgtcgttggg ggtagtgtaa gcgactgaaa aacaaacgcc cctgaaattc gtgtctatcc
     4681 gccaagactt tcacgaaaat acaggggcga tgtcggtcga taacctggaa ggaatcgtaa
     4741 cacatgagcg ccgcgcttca atacttcgaa gaaaa
//
