LOCUS       CP077358                2102 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1380 plasmid unnamed5, complete
            sequence.
ACCESSION   CP077358
VERSION     CP077358.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357522
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1152.65x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:44:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,077
            CDSs (total)                      :: 4,959
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 71 of 152
            Pseudo Genes (incomplete)         :: 88 of 152
            Pseudo Genes (internal stop)      :: 34 of 152
            Pseudo Genes (multiple problems)  :: 38 of 152
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1380"
                     /culture_collection="FDA:FDAARGOS 1380"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            join(1300..2102,1..163)
                     /locus_tag="I6L10_25345"
     CDS             join(1300..2102,1..163)
                     /locus_tag="I6L10_25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QXB65839.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            167..433
                     /locus_tag="I6L10_25350"
     CDS             167..433
                     /locus_tag="I6L10_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXB65840.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          1694..1925
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 ggcgctaaaa aacgtcctcc agttggatcg agaaaccaat gaggatcttg tcattgccga
       61 cgatgtaggg gatggcactg atgacgggaa gcggacggcg tttgtctggg attcaggtaa
      121 acggcgttac aaacgcgccc ctgagaagga taaatcggat taacgtatga atattaatat
      181 tgaatacctg aatggaaata agactattgg tttatttttt ttaagaagtg aagcggtgat
      241 tcctgacagg tttaaaaacc ttattttgct tattgatgga ttaagttttg gcacatttgg
      301 ttttcatccg cacgaaggtt ttgaggatga attaatttta tatattcaga aaacaaacga
      361 gagggtaaaa actctttttg tgaaaataga tttaaacggt atcaagtttg gatttttaag
      421 aacgcattct tagttctgga aaagagccag cggcaggctg aggtgatagg tacgagattg
      481 catgcaatct ctagtgctct gtctatcctg cattatcctc agcattatcc tcagcattat
      541 cctcagcctt gccaactcga caccaatgca ggatagacaa tccgatgtca aatgttaaca
      601 ctctgcgagt ggtacatttt ccccggatta tcgtcctgag cctgccgctg gctctctttc
      661 taccgcctcg ctttgctcgt tgctcaacgc ctcacagaca cggattaaaa tccgcatccg
      721 ttcaccgttt tttaaagtcc gttaaaagca tgatgccatc tccgagagtt aatctcgtca
      781 aatgctaaat cgtgggggtc ccctttgggg ttccgattta gtgattgacg acaccaccga
      841 ttaaaaaact tatgcggggt ggatggtttc acgaagtgag gccatccacc tgtaagacag
      901 ggttttgttt ttattccctg ttttggtgat cgggtgtgtg gaaaaggttg gggtaagccg
      961 ttcgggggtg cttgttttgg gggggttaaa attgtggtta ttttttgcgc aattctcgcg
     1021 cgtgatcctt gtatttatac ttaagggata aatggcggat atgaaatagt ggtttagccc
     1081 agtaatgacg aggctttgag tgggttttga caggtcaaag aaaatggagc agaattgagg
     1141 cgtttttaat cggcgttggg gagtgcgtca acactcccca acatttcgaa tgtgtcacct
     1201 cagcggcaaa ctctggtgac atgtactggc tcgcaatgca caggtacgtg atgaatatac
     1261 cacatcaaat cacagcctgc ccagatcgga gcaggcttaa tgtcagaaga taaattcctt
     1321 tcggactaca gcccccgtga tgcagtttgg gatacccagc gcacgcttac cgattctgtc
     1381 gggggtatct accagactgc tgctgaattc gagcgctatg cactccgtat ggcctcctgt
     1441 agcggtttgt tacgttttgg ttggtctacc atcatggaaa ccggagaaac gcgcctacgg
     1501 cttcgtagtg cgcaattttg ccgtgtccgt cattgccctg tctgccagtg gagaagaacc
     1561 ctcatgtggc aagcccgttt ttatcaggct ctaccgaaaa tcgttgtgga ttacccgtct
     1621 tcccgatggt tgtttctgac gttaactgtc aggaactgcg agataggtga acttggaaca
     1681 gtccttacag caatgaatgc ggcgtttaag cgaatggaaa agcgaaagga gctatcacct
     1741 gttcaggggt ggatcagggc tacggaggtg acgcgaggta aggatggcag cgcacatccg
     1801 cattttcact gtctgctgat ggtgcaacct tcttggttta aagggaagaa ctacgttaag
     1861 cacgaacgtt gggtagaact ctggcgcgat tgcttgcggg tgaactatga gccgaatatc
     1921 gatattcggg cagtaaaaac taagacaggt gaggttgtgg ccaacgttgc cgagcaactg
     1981 caaagcgcgg ttgctgaaac gctgaaatac tccgttaaac cggaagatat ggcaaacgat
     2041 cctgagtggt ttcttgagct gacgcggcag cttcacaagc gccgttttat ctcgaccggt
     2101 gg
//
