LOCUS       CP077357                4082 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1380 plasmid unnamed4, complete
            sequence.
ACCESSION   CP077357
VERSION     CP077357.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357522
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1152.65x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:44:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,077
            CDSs (total)                      :: 4,959
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 71 of 152
            Pseudo Genes (incomplete)         :: 88 of 152
            Pseudo Genes (internal stop)      :: 34 of 152
            Pseudo Genes (multiple problems)  :: 38 of 152
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1380"
                     /culture_collection="FDA:FDAARGOS 1380"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            complement(join(3693..4082,1..1770))
                     /locus_tag="I6L10_25330"
     CDS             complement(join(3693..4082,1..1770))
                     /locus_tag="I6L10_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QXB65837.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCMNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            1949..2203
                     /locus_tag="I6L10_25335"
     CDS             1949..2203
                     /locus_tag="I6L10_25335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QXB65838.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     gene            2736..3437
                     /locus_tag="I6L10_25340"
     CDS             2736..3437
                     /locus_tag="I6L10_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QXB65836.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(1413..1771)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.722"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1772..1948
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          2737..3437
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..1857)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.677"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagctggcgg gtttcccggt cgacagaact cattaccggc atcagcgtcg gtagcagacg
       61 tccctgcgta aaatccacct tccttgctgc cagtgatggc atggctggct ggcggtcttc
      121 tccaagacag aaagactccg gccatgtctt cggctggcgg agattgcggg tatattgcgg
      181 ccatgtctgg caaacctgcg tcagaagttg ctcaaaggtc gcagaggaat aaacacgttg
      241 cagtggtgga tgttgaccga tttcgcttcg gtcacagata taaccactcc tgtcacgcat
      301 taaatcaaca cgctcaaaga ttctggtgcg gtatttctcc agctcgttcc cccagagcgt
      361 gtattctccg ttccggttgt gaagcagaag gtaacaggtc atttccccgt taccaggcat
      421 gggggcctcg ccataccccg taacgactgc gctcagtacc cctgaatcca taacggcatt
      481 gtcatttttc ctgagctgtt ccgctcgtcg ctccgcgcga ctacgttctg tatcctcctg
      541 ctccttccgg cgcaattcct cttcacgttt aagcaactgg actctgagag ctgcaaggcc
      601 ggatgccccc cactgctgcc actgtttttc aaatgccgtc tgactcgcgg gaatgtcctg
      661 tttctccacc ttccggtaat gctgtcgcaa tttttccatt tgctggctca gcgactggat
      721 ttttccctgt tcgctacggt attccggtgc cgcccattgc atcaggggaa tacgggaaaa
      781 accggacgaa aacggcgatg attttttcag gctggcagca tggctcatcg cttcctttcg
      841 ttcggtgttc agctcttccc agcgtctgcg cgtcgcctgc atatctgcca tcatcgacat
      901 ggcattactg gtgagtttcc atgccgcatt actgtccttc ggcaacgctt cgatgatgtc
      961 acgtagtgtg tacgaagttg cgttgtcagg aagcacgata ttcagaaggt cagccacacg
     1021 gcgggcaggc tctgtcagcc cgctgtttct gagttcggta cgggaaatct gtatttccag
     1081 gttaagtctg ttaatcagac gattctgttc gccgcgttct gtttcgatac cgcgtgtttc
     1141 catcgcacag gcagactttc cgaggtgaat accaggcgtt ttctcaagcc cctgtcgctc
     1201 gtatgaacga tggtctatct cttcctgata accggcgttc gccagtgccc tgttcgcatg
     1261 gtcagcccat gatgcacgcc acgtctcggc atgtgtccgg tcgttccagt ccctgacctt
     1321 tccaccgaat ccagcaggtc cgacagctct cgtggtcagc atgatgtgcg catggggatt
     1381 tgttttatcc atgtgatgga acgccacatc agcaatcatg ccctgactga caaagttttc
     1441 tctgacaaag gcgagaacgg tttcccgtgc ggcatcctgc ggtagttcgc gggggatggc
     1501 gagttcaatt tcccgcgcaa gttgtgagtt cttccggcgt tcggccttct caacggcatt
     1561 ccagagttcg gcacggttca tacaccaggc aggggctgtc gcgggagtca gaatcacagc
     1621 ttcagccaca ccgcttttgc gggtgtagtc gtgagtcagt ccggtacgct catcaagcat
     1681 cagtgaaccg gaacgatagg cagcagaagc aacggcgctg tagccgctgt ttctggagat
     1741 gattttcatg ctgagatgat aaatcgccat ttctgccgta atgccttttt gtggttttct
     1801 ttcagccccg cagggcgcac acaatcgaaa cggagtggag attgtataag tgcgcacttc
     1861 gtgattttcc atgcattatg ccagctcagt ttttaaacta ccagtgacgc gctaaaatgc
     1921 gatttttcat cgcgcacgga gttacatcat gagcagaaca ctcgaacaga agattgctga
     1981 tgctgaggcc agattacagc gcctgaaggc gaagagcagg agtctggaca cagcgcagaa
     2041 ggtcattgtg ggtgcagcat tgctggcaaa ggtcagaaag ccggaggagg tgcagttgcg
     2101 tgcctggtta ctccagttcc tgaaggcaga ggtgacacga caggctgatg tgacgcgcat
     2161 actgccgctg attaacgagc tggaagcgct gccaggacag tgattgtatc gtccgctcgc
     2221 cgcagtccca gcgaccgagc gttagcgagc gagtgggcga ggaagcggaa gtgaatccgt
     2281 cgcctgattg agcacatacg cacggttttt cgggaggtga tatgcgctga acccgcatga
     2341 atactggatt tccacagggt tggagggggt gtattcggaa cttgcaattc tgcttttttg
     2401 caacgcattg tttttattgg ttttttgttt tcagcactcc aagttatcca caggctcgtc
     2461 gttaagtttt tagatcttgt ttttaagatc ttgtttttaa tacttgtttt agcgccaaaa
     2521 ttccttatag aaaacaaaat gttacaacgc tataggtaat atgatttaag gtaaaggtaa
     2581 tatgatttaa ggtgtttagg tggaggaatt taaggtaaag gcaatatgat ttaaggtaat
     2641 attcacagaa aaagggatat taaaataaaa aggcattgcc tttcgttgag ggtatgttta
     2701 tcttttattc attacctttt ggggagggat gctctatgtc ggatctaatt gcctataaat
     2761 ccaatgcgct ggttgaagcc agttataaat tgaccctaca ggaacagcga tttttgttac
     2821 tttgcatcgg tcgattgaag tctggggctg atgcagaatc tccaaaattg caaaagacaa
     2881 tgaccattac agctgcggaa tatttcgatt cattccctga catggggcgg aaaaatgccg
     2941 aggttcagtt acaggaagca attgatcgtt tgtgggacag gtcaatcatt ctcaaggatg
     3001 acgagaaacg tgaggagttc cgctggatcc agtatcgggc acagtacgct aaaggcgagg
     3061 ccagagcaca aataacattt tctgacgcag taatgcctta tttgacacaa ctacaagggc
     3121 aatttactag agttgtaatc aagaatatat ctagattatc aagaagttac agtataagga
     3181 tatacgaact cctccagcag ttccgcagta ctggcgaacg tattatcgct ctcgatgatt
     3241 ttcgctcaat gcttgatata gagcataaat atcaaacata taaatcactt aaccagcaat
     3301 tattaagacc ctgcattgac gaactgaaca aaaaaagcga cctggctgta acggttgaga
     3361 cgatcaaaaa ggggcgcacg gttgtagccc tgcattttcg cttcaaggaa gacaaacaaa
     3421 tcaagatgac gatctaaaat cgattttaag gcacctaaaa cgctccctgc tgcgtttttt
     3481 ttgtgttagt agtgcgaagg tactattgaa gtgaaaaatt gcgtcaaaag cgctctaatg
     3541 cagcgaaaaa agaaccccca ttttttgggg gcggaaaaac aaaactatgc ttgcattatg
     3601 tgtgtgcttc ttgcattcat ccctgtcgat gaacatcgaa gaacatcgac gaacataaca
     3661 cgatgttaaa caaaacaaaa tattgttcac attcactgtg accaaccgcg atcatgtctc
     3721 ctctcggtgg cgtgtgttcg tgcatcttcc tcgcgccgcc attcgtccca ggtacgatta
     3781 aaacccagcg agcggaacag atgatccggc tgtttttctg gtgtgttgac ggcagcaaaa
     3841 agcggtgcct cacgggtttt cagatgaaac tccataagtt ccggcgcagg tgatatgtgg
     3901 ctgtctgcat cgtgatttac ggcaacacca taccctgcat accgtaaccc cttatcggtc
     3961 agtgcactga tgaccagctg gctgttatcg gctgagggaa cggcaaacgc gggatacagc
     4021 gtaccattaa gccggacaac accgcccaga gagtcatcca cgcccatgac cagcaaaagc
     4081 tg
//
