LOCUS       CP077356                1551 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1380 plasmid unnamed3, complete
            sequence.
ACCESSION   CP077356
VERSION     CP077356.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357522
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1152.65x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:44:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,077
            CDSs (total)                      :: 4,959
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 71 of 152
            Pseudo Genes (incomplete)         :: 88 of 152
            Pseudo Genes (internal stop)      :: 34 of 152
            Pseudo Genes (multiple problems)  :: 38 of 152
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1380"
                     /culture_collection="FDA:FDAARGOS 1380"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            362..488
                     /locus_tag="I6L10_25320"
                     /pseudo=""
     CDS             362..488
                     /locus_tag="I6L10_25320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
     gene            complement(932..1408)
                     /locus_tag="I6L10_25325"
     CDS             complement(932..1408)
                     /locus_tag="I6L10_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QXB65835.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(248..328)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.122"
                     /coverage="87.0"
                     /evalue="9.59e-28"
     Typing          442..700
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.0"
                     /coverage="99.0"
                     /evalue="1.09e-111"
ORIGIN
        1 aaacacacta aaaaaacaac aacttacaaa tcggcgtaaa agatctattc taggcaaggg
       61 tgttgcgcgc aacgcaacgc ccttttgggg tgtcggggcg aagccctgac ccagtcacgt
      121 agcgatagcg gagtgtatac tggcttattc cgctacaagc ctcgccagcc gtgatttttg
      181 ccagatttca cgctctcaac gctcaacagc tccatcatgg ttttgccgtc cgttcaccgc
      241 taaaacgcct cagaaatcgg gacaggatgt gcaaatgtaa taccgtcacc cgcgacgata
      301 ttccgtttgc cgtctggtca gggcttcgcc ccgacacccc gcatcagtgc gtacgtgctc
      361 aatgtggttt ccggttctct tccgcttcct cgcccactcg ctcactacgt tcggtcgctg
      421 gggctgcggc gagcggtgtc acctctcgca tgttgtcggt gattccgttg ttgttctttc
      481 ggggatgagt cgccagatag cccctcaaac gcgctcagaa gctctcagac acacgaaacg
      541 ggttttaaat gcccgtcagg gcatggaagg cgcgaaaaac gcttacagag gctctcagga
      601 ggtcaggcgc gccgataccc ctaaccaggg gctttcaggt cgctaggaac cgcgtcagcc
      661 ccctgcaaag cctatttgca cggcacaggc agctattcgg ctggttggtg gtcgtagacc
      721 gtcggtgtta cagcgatggg ggtctggggt tctccccaga cggtttccgg ttgcgctcaa
      781 ggcgctgccg gaaccgtggc ggcgcggtcg ttctggggat cggggagcaa aaacaagcaa
      841 gggattttga ggcggcaaaa gagcaatgca ggatagacaa tcatggtcaa aacgttaaca
      901 cgtagcccct gattgtccgc ctgccgctgg ctcaatttga aaacatggcc atgattcggc
      961 gctgaatatc accgtctttg ttgaccagtg ttttagtgac aaacgctgca tggtttttcg
     1021 tagtgttgta tcccttccag acttcatgtg gatcgagggc atacgcacaa accttaccag
     1081 caacggggat tctaactaat gcattttttg attctaagta attgagccac cgaataattg
     1141 tgcttctgct aactccaagt ttttcagcta ggaattgctg atccgcaacg acagcgccac
     1201 acgtaggatc gatatgctcc gctaaaaatg agtacagctt cagtgctgat atgccttgac
     1261 tatccttcag cagctctcga acgcgttccc aaccttttgg ggatacctgt gtaaaccttg
     1321 aattcttctt tgcttcttcg agatcctcgg tgtttttctt tgcctcatct gaaagttgtt
     1381 tcagtcgttt ttctgaatca ataaacatga ttttttccct ctgaaaatcc catgcactaa
     1441 cgttggttca aatctaagcc atcatgacac atctgtgcaa caccttgttg cgtgttgtcg
     1501 catccaggat gcgacaacgt tgtgtcaccc tggatgcgac aggtaaacaa a
//
