LOCUS       CP077355                4245 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1380 plasmid unnamed2, complete
            sequence.
ACCESSION   CP077355
VERSION     CP077355.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357522
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1152.65x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:44:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,077
            CDSs (total)                      :: 4,959
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 71 of 152
            Pseudo Genes (incomplete)         :: 88 of 152
            Pseudo Genes (internal stop)      :: 34 of 152
            Pseudo Genes (multiple problems)  :: 38 of 152
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1380"
                     /culture_collection="FDA:FDAARGOS 1380"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            join(4122..4245,1..92)
                     /locus_tag="I6L10_25285"
     CDS             join(4122..4245,1..92)
                     /locus_tag="I6L10_25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QXB65830.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            221..739
                     /locus_tag="I6L10_25290"
     CDS             221..739
                     /locus_tag="I6L10_25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXB65831.1"
                     /translation="MKCFWACSVSVVLVMSFTVASAENITHTKPLARSDLYLTCKGYQV
                     KGASVINVEKPSYLEGTGEQVEFAIISLGDASDGLKVFFDSWGFSPFGLIESHVMHEHW
                     SLVHVGGRVSVKGEKIDYSFVDNQPQEGGSYKPKTGVFLYGCINSDKRPSWVEKIYTRT
                     GVFRGGIED"
                     /name="hypothetical protein"
     gene            complement(969..1556)
                     /locus_tag="I6L10_25295"
     CDS             complement(969..1556)
                     /locus_tag="I6L10_25295"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXB65832.1"
                     /translation="MIRNIFFASAIVVAISGCTAMDKLAGMGVVSQQTSTFDNSTVIDV
                     SPNSLYDPNSTWGTQLQLGAIWSSSQPDQVGLILSYNSNVSNGSSAYLGFIGIDINIDG
                     KISSYKAQQLTNLSNSGYNTVSKTIYTSSKNTVVIPYEVLEKMVSAKNCRLRIHTTEGN
                     QDIDFSAASIPGGKATAIVSIKEFMTKVAKVK"
                     /name="hypothetical protein"
     gene            complement(1871..1984)
                     /locus_tag="I6L10_25300"
     CDS             complement(1871..1984)
                     /locus_tag="I6L10_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QXB65833.1"
                     /translation="MHEPPVQSDRCALSGNYHLESNPERHAKAPLAAATDN"
                     /name="replication initiation protein"
     gene            2632..2955
                     /locus_tag="I6L10_25305"
     CDS             2632..2955
                     /locus_tag="I6L10_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QXB65834.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2894..3658
                     /locus_tag="I6L10_25310"
                     /pseudo=""
     CDS             2894..3658
                     /locus_tag="I6L10_25310"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3633..4118
                     /locus_tag="I6L10_25315"
     CDS             3633..4118
                     /locus_tag="I6L10_25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QXB65829.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLTASGASILWWQGQQIL
                     DNYTTIREQKRTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     Typing          2346..2518
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.828"
                     /coverage="100.0"
                     /evalue="9.34e-71"
     Typing          complement(1876..2421)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.078"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          2946..4243
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.841"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gggagaacgc ggcgctgagc gagcgcgtca cgggcttgag caggcaggtg cagagtttga
       61 gcgatcagct gcgccggttg tcgaaaggct gaactcgatt gaagcacaca ggcagcagga
      121 gcgggcggcg cagcatcaga aaacgctgga gctggaacga tcgcagcggc agaaaggttt
      181 tgagcttgga atgtagttaa tcaatgaaaa gggatgcgtt atgaagtgtt tctgggcttg
      241 ctcagtctca gtagttttgg ttatgtcatt tacagtcgct tctgcggaga acattacaca
      301 cacaaaaccg ttagccagga gcgacttata tcttacctgt aaaggttatc aggtgaaggg
      361 ggcgtctgta attaacgtcg agaaaccttc ttaccttgaa ggtacaggtg agcaggttga
      421 gtttgcaatc atttctctcg gtgatgcatc tgatggactc aaggtttttt ttgattcatg
      481 gggtttttct ccttttggat tgattgaaag tcatgtaatg catgagcact ggtctttagt
      541 tcatgttggg gggcgtgttt cagtcaaggg tgaaaagatt gattattcat ttgtagataa
      601 ccagccacag gagggtggga gctataaacc caaaacaggt gtgttcctgt atggctgcat
      661 taactctgat aaaaggccat cttgggtcga aaaaatctac acaagaacag gcgtgtttcg
      721 agggggcata gaagactaaa atactgcgta cagcatgaaa attcattgaa cagagtgtta
      781 gttcattttc gtgcatggct atgcatacac gcaaaactta tcagcgcgat tccgggcggt
      841 tttcggtgag aagacgggct cttttgtggc tcaggacgga aaaacgccct gagcgcgtct
      901 gagcggtgcg cccaagggat gttatggtaa atagtgtgaa gtgccgcctg taatggcggc
      961 ttagttacct atttaacttt tgccacttta gtcatgaatt ctttaatgga aacaatcgca
     1021 gtagctttcc ccccagggat acttgcagct gagaaatcga tgtcttgatt accttcagtt
     1081 gtatgtatgc gaaggcggca gtttttagcc gacaccatct tttctagcac ttcatatggg
     1141 ataacaacgg tatttttgct ggatgtgtag atcgttttgg atacagtatt gtatccactg
     1201 ttacttagat tagttagttg ctgtgcttta tagctagata tcttgccatc aatatttata
     1261 tctattccta tgaaacctaa gtatgcggaa cttccattag atacatttga attgtaggaa
     1321 aggatcagcc ctacttgatc tggttgggag ctggaccata ttgctcctaa ctgaagttgt
     1381 gttccccaag tgctgttcgg gtcgtatagc gaatttggag agacatctat gacagttgag
     1441 ttgtcaaaag tggatgtttg ttgagaaacc acacccattc ccgccagttt atccattgct
     1501 gtacagccag agatggctac tacgatcgca gaagcaaaaa aaatattcct tatcatttcg
     1561 ttatcctatt tttttcttgt taccagagag tgaaaaaagc atcgtattac cgaaactatt
     1621 ttgcacgcga ttttcttttt aagaaggatt ttgaaaggat acgggcagat cttcttgaga
     1681 tccttaagcg agcgtcgtaa tctcttgacc acgaaacgaa aaaaccgcct tgcagggcgg
     1741 tttttcgaag gttctcagag ctaccaactc tttgaaccga ggtaactggc ttggaggagc
     1801 gcagtcacca aaacttgtcc tttcagttta gccttaaccg gcgcataact tcaagactaa
     1861 ctcctctaaa tcagttatca gtggctgctg ccagtggcgc ttttgcatgt ctttccgggt
     1921 tggactcaag atgatagtta ccggacaagg cgcagcggtc ggactgaacg gggggttcgt
     1981 gcatacagtc cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga
     2041 gataaacgcg gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag
     2101 cgcacgaggg agccaccagg gggaaacgcc tggtatcttt aagtcctgtc gggtttcgcc
     2161 accactgatt tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa
     2221 acggcttcgc cgcggccttc tcatttccct gttaagttcc ttcttggcat ttaccaggaa
     2281 acttccgccc tattcgtaag cccgcaccgc tcgccgcagc cgaacgaccg agcgcagcga
     2341 gtcagtgagc gaggaagcgg aatatatcct gtctcatata ttctgcttac gctccgctgc
     2401 tgcatttttc tcctgccaca tgaagcgctt cactgattcc cgcacccgtg ccaacatagt
     2461 aagccagtat acactccgct agcgctacgt gactggttca gggctgcgcc ccgaaacccg
     2521 ctaaaaccac tgacgcgcct gcggcttgtc caacaccgcg ccgaccggga aaagatttcc
     2581 cgcccgtccc ggcgttatca ggaggccgga ttggcagaca aacgcagcaa aatgctcacg
     2641 atgtgggtga ccgaagatga gcaccgccgt ctgctggagc gctgcgacgg taaacagctt
     2701 gcagcctgga tgcgccagac gtgcctggac gagaaacccg cccgcgccgg caagcttccc
     2761 tcgctctcgc cggcgctgct tcgtcagctt gccggcatgg ggaacaacct caaccagatt
     2821 gcccggcagg ttaacgccgg tggtggcagc gggcacgacc gcgtgcaggt cgtcgccgcg
     2881 ctgatggcca tagatgccgg actcgagcgg ctgcggcatg ccgtgctgga aaagggtgca
     2941 gacgatgatc gttaagtttc acccgcgagg gcgcggcggt ggcgccggtc cggtggatta
     3001 tctgctgggg aaagaccgac agcgcgacgg cgccaccgtt ctgcagggga agccggaaga
     3061 agtcagggag cttatcgacg cctcgcccta cgcaaaaaag tacacatccg gcgtcctgtc
     3121 ctttgccgaa aaggatttac cgcccgggca gcgcgaaaag ctgatggcga gctttgaacg
     3181 ggttctgatg cccggactcg ataaagacca gtacagcgtg ttgtgggttg agcaccagga
     3241 taaggggcgg ctggagctga acttcctgat cccgaacacg gagctgctga ccggcaggcg
     3301 tctccagccg tattacgacc gcgccgaccg tccgcgcatt gatgcctggc agaccatcgt
     3361 gaacggcagg ctggggctgc atgacccgaa cgcgcctgaa aaccggcggg cgctggtcac
     3421 gccgtccgcg ctgccggaaa cgaagcagga agccgctcag gcaattacac ggggtttact
     3481 ggccttagcc tcgtccgggg agctgaaaac ccgccaggac gtcactgagg cgctggaaag
     3541 cgcaggtttt gaggtggtac gcaccacaaa aagcagcatc agcattgccg acccggacgg
     3601 ggggcgaaac atccgactga agggagccat ctatgaacag tcttttaacg ctggcaaagg
     3661 acttagagca gaaatcgaaa gcgcagcagc agagtaccgg cgagatgctg aaagccgcat
     3721 tcagcgagca cgagaagtct gtcagagcgg aactgagcga aagcgagaag agaatcagcg
     3781 ccgccatcct cgaccacgac cggaagctgt cctcagccat gagccagcgc acgaaaggca
     3841 tggtgcgtat ggtcagccag acgtggctga ccatcgtcct ggtgtctgca ctgctgacag
     3901 catcgggcgc aagcattctg tggtggcagg ggcagcagat actcgacaat tacacgacta
     3961 tccgggagca gaagcgcacg caggccatgc tgtccgagag gaacagcggc gtgcagctct
     4021 cgacctgcgg agagcagaga cgccgctgcg tgagggtgaa cccggaagcg ggacggttcg
     4081 gagaggattc gagctggatg atactggcgg ggaaatagca catgacggag ctggaaaaac
     4141 agttgctgag cgcattagag cagctacagc aggactactc gaaaaggctg gacgagtggg
     4201 agaacgcctt cgcggaatgg cggacgatgt ctggtcttat gcaac
//
