LOCUS       CP077352                2924 bp    DNA     linear   BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1389 plasmid unnamed10.
ACCESSION   CP077352
VERSION     CP077352.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357531
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 725.88x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 17:11:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,169
            CDSs (total)                      :: 5,039
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 329
            CDSs (without protein)            :: 329
            Pseudo Genes (ambiguous residues) :: 0 of 329
            Pseudo Genes (frameshifted)       :: 134 of 329
            Pseudo Genes (incomplete)         :: 190 of 329
            Pseudo Genes (internal stop)      :: 31 of 329
            Pseudo Genes (multiple problems)  :: 23 of 329
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1389"
                     /culture_collection="FDA:FDAARGOS 1389"
                     /db_xref="taxon:562"
                     /plasmid="unnamed10"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            1675..2589
                     /locus_tag="I6L19_25845"
     CDS             1675..2589
                     /locus_tag="I6L19_25845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QXB53047.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     Typing          1676..2450
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.907"
                     /coverage="83.0"
                     /evalue="0.0"
ORIGIN
        1 gtgaaccaga aaaaatcccc cgttggcata gggggatatg taaggagaaa taacttcgca
       61 ggtgttcttg tttgtataac gtgaaaagac tgcacgcaac ttaataagtc tgccgtactt
      121 actttcagtt ttcaacataa ggtttataac aatgtatgga atggaagtga gctttaaggt
      181 ttcagctcta aaaaataaaa tatttattag ttggctggtt gttgttcttg ttctgtatgt
      241 tataaacact atcgacatat tatgaccggg aacattcctt accccctctg cgctcgcaga
      301 ggtttttttt tgtcattaat taataatgaa ctgaaatgca gttccgatac ctccatttgc
      361 acattatccc tgacatatgg taccggaacc gcattaattt tatgttataa gagtttattt
      421 ttaatgtcca gatggtttaa tagtgtttat atgtaattag ctgtatggat agtactggtt
      481 ttattgtata acttgtcgtt ttttttattg tttttgtgtg gtgtgctaat ctttaagtta
      541 taaaaaaacc ccttggaata caggggcaaa agtaaggaaa acaaatggat gctcagtttg
      601 tgtgacactt taaactaatg gaactataga gtggaggttg ttacagtcgc ttttataaaa
      661 cagcaaccag tttttttcaa tagtctgatg atggttgtga cagttttgtg atattgttca
      721 aaatgtttct tgttgaatgt ttgttttttg gtctattggt gtctggtttt attttttctg
      781 ctattacata tcaagaaatg tgattggtgt agcaagagtg gaatgcgatg tggataaact
      841 aattggttgt cgaatctaat ttacttagaa aactacactt gtttttgtgt atgaatttag
      901 tgaatttgtc tgtctatgtg ttttcgtgca tttataaatc cttcgtcagg atcttaaatt
      961 tagtgttgaa tatgtagggc ctgtacggta attattattt agcgtagcta cttcatcgtt
     1021 gttcgaacac aaccaacaac ctgtaattgt tgtttctctt ggttccccac ttttgtgggg
     1081 ttttttatct cttccgtgca tcgtaaagcg cggactcctt ctggaaaggt tgttttaggg
     1141 catcactgag gcattttttg cgtttgtgaa tattgagcat ccgataacta aaatatttcc
     1201 catagactct gattcttgcc accgacccca ggtcggtttt tttttgtcct ggcgaaacgt
     1261 gaagctctga aacagaaaat ctccgggcag aaccttccat ccggggggag aaaacttgtc
     1321 gttttgagcc gtccggtgtt cagaacgcac gaaaccgatc gcacatcgct ttcgtgcata
     1381 gttatgcaac gccctgaaaa cgattctgac gcgtttttct ggtttggcct ggtgttttct
     1441 tgtctttttg cgttttttgc gccagaaagc gactgagggc gttttaaggg gtacgtacaa
     1501 cgggagttat ggtaaatgga tcgggttttc gggaaggttc gacaggattt gccgttgggt
     1561 gtagtgtaag cgactgaaaa acaaacgccc ctgaaatcat gtccagatcc ggcaagattc
     1621 aacatgaaat acagagggcg tgtaggtcgc ataacccgga gagaatcgta acacatgagc
     1681 gccgcgcttc aatacttcga cgaaaattta ccccatcgtc cctatcacac ggatgatctc
     1741 gcttttggtc ttcgcatctc cggcaaaggg cgtgcgcttc ttgcgcggta catacagcag
     1801 aaccagcctc atgcgcagtt ctggctggtt tttgacgttg accgcgaggg ggctgcgatt
     1861 gactggagcg accggaacgc tcccgcgccg aacatcaccg ttaaaaatcc tgtgaacgga
     1921 cacgctcacc tgctctacgc actcaacatc gccgtgagaa ccgcgcctga tgcctcggtt
     1981 aaggcgctga aatatgccgc agcggttgaa cgttcgctgt gtgagaaact ttgtgcggat
     2041 gtgaactaca gcggcctgat ttgcaaaaat ccgttccacc tggaatggct ggtgatggag
     2101 tggcgcgagg aagcctatac cctcgatgaa ctggctgatt atctcgattt gagcgcctca
     2161 gcgcgtcgta gcatcgataa acattacggg atggggcgaa actgccacct gttcgaaatg
     2221 acgcgcaaat gggcttacag ggcgattcgt cagggctggc cagcgttctc acagtggctt
     2281 gaagcggtga tccagcgtgt cgaaatgtac aatgcatcgc ttcccgttcc gctttcactg
     2341 gctgaatgtc gggctattgg caagagcatt gcgaaataca cgcacaggaa cttcacgccg
     2401 gaaactttcg cacagtatgt ggctgatacg cacacgccag aaattcaggc tgcacgcggt
     2461 cgcaagggcg gttctaagtc taagcgcagc acagtagcta catctgcacg cacgctgaaa
     2521 ccgtgggagg cattagggat cagtcgcgcc tggtattacc aactgaaaaa acgaggtctc
     2581 gtagagtaga ccaaataagc ctatatcaga taacagcgcc tttctggcgt ctttttgagc
     2641 agcaggtttt ttgccgtttt tccccgttca gccctgcaat ggcgcggctt tccgcgtgat
     2701 tgtggtggta gtgctcgccg cagtctcatg accgagcgta gcgagcgaat gagcgaggaa
     2761 gcaccaggga atcggaacac ggatggaggc acttacgcgt ctgggtttgg ggatgtctcc
     2821 agatcgtact taagttcctt aagtgcttgt aatatcctgt cttttttata gcatagctcg
     2881 cctgtttttg tgtctaccag tcctgcttcg atcagtagcc cagc
//
