LOCUS       CP077351                5494 bp    DNA     linear   BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1389 plasmid unnamed9.
ACCESSION   CP077351
VERSION     CP077351.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357531
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 725.88x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 17:11:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,169
            CDSs (total)                      :: 5,039
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 329
            CDSs (without protein)            :: 329
            Pseudo Genes (ambiguous residues) :: 0 of 329
            Pseudo Genes (frameshifted)       :: 134 of 329
            Pseudo Genes (incomplete)         :: 190 of 329
            Pseudo Genes (internal stop)      :: 31 of 329
            Pseudo Genes (multiple problems)  :: 23 of 329
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1389"
                     /culture_collection="FDA:FDAARGOS 1389"
                     /db_xref="taxon:562"
                     /plasmid="unnamed9"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            1..411
                     /locus_tag="I6L19_25805"
                     /pseudo=""
     CDS             1..411
                     /locus_tag="I6L19_25805"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            389..919
                     /locus_tag="I6L19_25810"
     CDS             389..919
                     /locus_tag="I6L19_25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QXB53040.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            926..1159
                     /locus_tag="I6L19_25815"
     CDS             926..1159
                     /locus_tag="I6L19_25815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QXB53041.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1639..2691
                     /locus_tag="I6L19_25820"
     CDS             1639..2691
                     /locus_tag="I6L19_25820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXB53042.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFICIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            2693..3559
                     /locus_tag="I6L19_25825"
     CDS             2693..3559
                     /locus_tag="I6L19_25825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="QXB53043.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(4762..4953)
                     /locus_tag="I6L19_25830"
     CDS             complement(4762..4953)
                     /locus_tag="I6L19_25830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QXB53044.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5018..5341
                     /locus_tag="I6L19_25835"
     CDS             5018..5341
                     /locus_tag="I6L19_25835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QXB53045.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5331..>5494
                     /locus_tag="I6L19_25840"
     CDS             5331..>5494
                     /locus_tag="I6L19_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="QXB53046.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELID
                     ATPFAKKYTS"
                     /name="nuclease"
     Typing          4587..4668
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          3943..4130
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          2..1263
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          4986..5338
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.153"
                     /coverage="100.0"
                     /evalue="4.74e-70"
ORIGIN
        1 ggacggcttg agctgaattt tgtcattccg aacatggagc tacagaccgg aaaacgcctc
       61 cagccgtact acgaccgggc agacaggccg agaattgatg cctggcagac gctggtgaat
      121 caccattacg gtctgcatga ccccaacgcc ccggagaacc gccggacgct gacccttcct
      181 gataacctgc cggaaacgaa acaggcgctt gctgagggcg tcacacgcgg gatagacgcg
      241 ctttaccatg ccggagagat aaaaggccgt caggatgtga ttcaggcgct cacagaggcg
      301 gggcttgaag tggtcagggt gacgcgaagc agtatcagca ttgcagaccc gaacggcggg
      361 aggaatatca gactgaaagg agcgttttat gagcaatctt ttacagacgg gcgcggagtt
      421 cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa atgctgaacg acgagttcag
      481 gaagctcgaa gaatctgtaa acagggaact gacctcaaac gaatctttaa tcaggaacgc
      541 tatcaacgac catacaacag ccctgaaaga actgctggag aggtatcaga aaacgaccgt
      601 cgatacgatg gacgcgcact ggaaaaccgt actgaaaatg agcgtgaaac gctggctgtg
      661 gctgattatc gtctcagtac tgatgtttgc cacgaccggg agtctgctgt ggtaccaggg
      721 catgaagata aacgcgaata tgaatattct ccgcgagcag aaagagagtc tggagaagct
      781 gaacgcgaag acatggggcg tgcgttatca cgaggacagc aacggacgtt ttctggtgtt
      841 gccgaagggg atgaaagccg aaacgaactg gacgaaggac aacggcaaac tgaacgcagt
      901 gagactggtg caggagtgac agagcatgac ggagctggaa agacatttgc tgaacgcatt
      961 agagcaactg caacaggact atatgcagcg gctgaacgaa tgggagaacg ccttcgtgga
     1021 attgcagaaa atgttttcgc ttacgcaacg ggacaacgcg atgctgaacg ggcgagtcat
     1081 gcagttgagt cagcaggtgc agcacttgag cgagcggaca gaacgcttga gccagttata
     1141 cagcgagaac aggagataag ggatgaacgg ctgatgcagg aacgcgaaca tgagttatct
     1201 ctggagcggg agcgtcagcc ggaaatccag gagcggacac tggatggccc ttcgctggga
     1261 tggtgaaacc atgaaaaatg acgtgttcag tggattaagg cgggctaatg tggcctgtag
     1321 catctggttg cataggtatt catacggtta aaatttatca ggtgcgatcg cggcagtttt
     1381 tcgggtggtt tgttgccatt tttacctgtc tgctgccgtg atcgcaccga acgcgttttg
     1441 gcggtgcgta caatgcgacg ttatggtaaa tggagcggat tttcaggaag gagtgatact
     1501 aacaactcga acactgtgca aataatgatt tttttaaaat atcggtaacg ataataaatt
     1561 atttcgtttt acgccggtaa taaatataat gtagtttcag tatataaaaa atatatgagg
     1621 tgtcttgtgg gagctttcat gaatcttcaa caacgtataa ataaactacc acaattatca
     1681 tccagttttt catttggaaa agatattgat aatattcatt catttatctt taacgaaact
     1741 tctaaagata aaattgaaga tttactaaga aaatgggtgt caggcaatca gccctgtgta
     1801 tttggtaaat tggcaagcaa gaaaattaaa gggcttgatt tccatttatc tattgtaaat
     1861 agcccccaac tatataatga tgatggtcat ttgtttgatt ttttgcgtaa tgaaagagtt
     1921 cgttttaaag aacgcgcaag aagaggagag gtcagtgcgc atctcattta ttttattcat
     1981 ccacaacttg cctttgctcg tccatcagaa gagttagtgg acatacaaaa atatatttgt
     2041 tcgctgcata tgccagagtg ctatccaata aaagaggatg ttatttatac tgaaagcgta
     2101 ccatttcagg ataaagatgg gctaaaaata tataaggctg gtgttaatgt tttttactct
     2161 tctgctcata gaacaagaaa tcatgataga agaataccag ggggaatact tatttctgta
     2221 aatgccccgg ggcattttat gaggctggcg atagaaaaag gtttctataa agatcaagaa
     2281 caagcgttag cggatattcg taacatgact attcagtctg ttggtaaggg tgggtattca
     2341 catccggaag ggatttctac aacatggcat tcagaatcta atttggatcg ttttggatgc
     2401 ccagtgcaca ccggtaatag ttcttattac agcggttttt atcatactga tgtattaatt
     2461 ccaggagaat taactaagga tgaaagattg ctccatgaaa tagaaaatag tgatccaatg
     2521 atcttcaact ggaatgtatt gttttatgtt tcattagaag agtttcctat agatgatcct
     2581 tattatggag agtttatttg tattcctgta gatgatgcat ctatgttttt taactccttt
     2641 caaccaagga aatttgaaaa caaacccctt tatgagaaag aggatgatta atatgattaa
     2701 aggaatgctt tttgcttttt tagcagcgtt ctcatggggg gcagctatcg taatgagcaa
     2761 aaaaggagtt gaagaactag atgccggagg ggtgtttttc tggcagattt gtagtgcagt
     2821 aatactatct tggatagttc tttttataaa aagaaaaaaa ctcccattaa actcaaaagc
     2881 gatacttgct tatgcgacag gcgtttttga accattctta gcgtatactt ttacactcta
     2941 cggactttat tttatttcag caggaagcgc atcaattatt ttttcacttg aatcagtgtt
     3001 tatattggtg ttgtctgttc ttatttgctc agttaaaatt aattccccgc aatattttat
     3061 attgttcctt ataggtgcaa ttgttggcag tattttggta gccttgccag acatcagtaa
     3121 tggatcgaat aatattgttg gatattgtct tgttgttgcc ggtgtgttat cggcagcatt
     3181 ttatgttgtt atatcgtctc gccttattaa atcgtttgag cctatcacat tattgagcgg
     3241 acagttgaca ttctccctta tactgtcatc gctctttatt tttatatcta aatcatcaat
     3301 ttatcttccg ttggaatccg tgattattgt tttttcatct ggaatattac aatatttttt
     3361 agctttttgt ttttacttga actccttgcg ttggctccca gttcatttgg cagggataat
     3421 gttgtacttt attccgctag ttgcattatt tttgtcatgg ttgtttttgg atgaacaatt
     3481 cacattctta cagggggtag gatttattgt aactgtgcta agtgtatatt ttcttaacag
     3541 aaaatactca gaggagtaac attctgttca ggattgcccc ccggcctcca tcgataccgt
     3601 gccaaatgca cgtaaaaaac gggagcgttt ctggtgtcgg aatgtaacca agggggtcag
     3661 aggcgcgcta aaacgctctc aggggagtca aatagagacg tttgtaacaa aaagatcaaa
     3721 ggatcctctt gagatccttt ttttctgcgc gtaatctgct gcctgtaaac gaaaaaaccg
     3781 ccttgcaggg cggtttgatc gaaggttaag tcagcttgca gactgcttaa ccaggtaact
     3841 gtcttatgcg agacgagtca ccaaaattgt tcttctagtg tagccgcact ccggcagcca
     3901 cttcaagtac tctctaaata tctcgcacat gacttttacc aatggctgct gccagtggcg
     3961 ctttgtggtg tcttttccgg gttggaccca agagaacagt taccggagaa ggcgcagcag
     4021 tcgggctgaa cggggggttc ctgcatacag cccagcccgg agcgaactgc ctaagtcgcc
     4081 ccgagatacc agaagacgtg agctatgaga aagcgccacg tgtcccgtaa gggagaaagg
     4141 cggaaaaggt atccggtaat cgatatggcg ggaacgtggg ggcgcaggag ggagcgattc
     4201 accggaaacg gtgatgatct ttaagccacg tcgggtttcg ccaccgctgg cggatttcat
     4261 ggttgagccg caaggctccc acagatgcac cgaaaaagtg tctgttttgt gaagtccgtc
     4321 aggggggcgg agcctatgga aaaagcgcct ccggcgcggc ctttcccggg gtttcgtttt
     4381 ttgcgcacat gttctttcct ggtttatccc cgggttctgt ggataacagg gttagcgttt
     4441 ttgagaaagg agagtccgct cgccgcagcc gaacgaccga gcgtagcgag tcagtgagcg
     4501 aggaagcgga agagcgcctg aactggcatt ttctccttac gcatctgtgc ggcatttcac
     4561 accggacatg gcgcactttt catacaatcc gcactgatgc cgaatggtta agccagtata
     4621 cactccgcta tcgctacgtg actgggtcag ggcttcgccc cgacacccca aaggtcaacg
     4681 gcctgacgcg ccctggcggg cttgtctgct cccggcatcc gctcacagac aagctgtgac
     4741 cgtctccggg agctgcatgt gtcagcggtt ttcaccgtca tccccgaagc gtgcgaggca
     4801 actgcggtaa agctcatcgg cgtggtcgtg aagcgattca cagatatctg cctgttcatc
     4861 cgcgtccagt tcgttgagct tctccagcag cgttaatgtc tggcttctga taaagcgggc
     4921 catgttaagg gcggtttttt cctgtttagt cactgatgcc tccgtgtaag ggggatttct
     4981 gttcatgggg gtaatgatac cgatgaaacg cgagaggatg ctcacgatac gggttactga
     5041 tgatgaacat gcccggttac tggaacgctg tgagggtaaa caactggcgg tatggatgcg
     5101 gcgggtctgc ctgggtgagc cggttgcccg ttccgggagg ctgccgacac tggcaccgcc
     5161 gttactgcgt cagctggcag ccatcgggaa taacctgaac caggcagccc gtaaggtgaa
     5221 cagcggacag tggtcttccg gcgaccgggt tcaggtggtg gccgcactga tggccatcgg
     5281 ggatgagctg cgccggttgc gtctggccgt cagggaacag ggggcgcggg atgatagtta
     5341 agtttcatgc caggggaaaa ggtggtggca gtggtccggt tgattacctg ctgggaaggg
     5401 agcgtaaccg ggaaggggca acggtgcttc agggtaatcc ggaagaagtc cgggaactca
     5461 tcgatgccac gccatttgcg aagaaataca cgtc
//
