LOCUS       CP077350                6063 bp    DNA     linear   BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1389 plasmid unnamed8.
ACCESSION   CP077350
VERSION     CP077350.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357531
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 725.88x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 17:11:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,169
            CDSs (total)                      :: 5,039
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 329
            CDSs (without protein)            :: 329
            Pseudo Genes (ambiguous residues) :: 0 of 329
            Pseudo Genes (frameshifted)       :: 134 of 329
            Pseudo Genes (incomplete)         :: 190 of 329
            Pseudo Genes (internal stop)      :: 31 of 329
            Pseudo Genes (multiple problems)  :: 23 of 329
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1389"
                     /culture_collection="FDA:FDAARGOS 1389"
                     /db_xref="taxon:562"
                     /plasmid="unnamed8"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            <1..521
                     /locus_tag="I6L19_25780"
     CDS             <1..521
                     /locus_tag="I6L19_25780"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXB53035.1"
                     /translation="VRFLLILRISTEDTPCTRRPQAIYCSTTTHRKKTPGSTTNMRISN
                     IEWLKKRLGFIRKLGEQTARQRQIIDLLDNEAGLTEQERKLLHVLATAEKNDLQAQENE
                     RKQTIQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTGELCYKKDRILQALKEIKYD
                     LETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(715..1650)
                     /locus_tag="I6L19_25785"
     CDS             complement(715..1650)
                     /locus_tag="I6L19_25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QXB53036.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDRVGAAIDWSDRNSPAPNVTVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLDADANYSGLICKNPFHLEWQVKEWREEPYSLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            complement(2961..4619)
                     /locus_tag="I6L19_25790"
     CDS             complement(2961..4619)
                     /locus_tag="I6L19_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007372656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="QXB53039.1"
                     /translation="MKIVIVGGVAGGASAAARARRLSEDVSIVVFERGSDVSFANCGLP
                     YHIGGKIPLRQSLILKTPEDFKSRFNIDVRIRHEVMSVDPVNKMVTVKNLTSGEVYTEE
                     WDRLLLSTGAAPVVPPLPGLQEAGVFTLRNLTDMDAIQAWIEQHNIAHTTLVGGGFIGL
                     EVMEALSERGISVTLLEMGEQVMAPVDPEMASALHQEIRSHGVDLRLRTALTEVLRTET
                     GFRVALSDGGFLQTDMVILAIGVKPESSLATGAGLAVGKRGGISVNACMQTSIPDIYAV
                     GDAVETPDLVFQEPANVPLAGPANRQGRIAADNMLDRHSLYHGSQGTSICKVFSLSIGS
                     VGANEKQLKAHGTRYEKVYVHAADHASYYPGATMISLKLLFSPDTGKILGAQASGKKGV
                     DKRIDVLAVVQRAGLTVNDLEHLELTYAPPFNSARDVVNQAGMVAANVIKGDTVICHVR
                     DVLQREPGSYCLLDIRSPAELKQFGEYPDALSIPLDSLRENLDKLPEDKEIIIGCQSGL
                     RGHVAYRMLQSHGFRTYNLSGGFITWQAVTESMSQ"
                     /name="FAD-dependent oxidoreductase"
     gene            4780..5127
                     /locus_tag="I6L19_25795"
     CDS             4780..5127
                     /locus_tag="I6L19_25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="QXB53037.1"
                     /translation="MPMNEDTKRQTDMKNAALGAADVLRGLANSDRLLLMCQLSQGEAN
                     VTELEAAVGITQPTLSQQLGVMRRLKLVETRRAGKMVYYRIGDNRIMTLLNTLYDLYCP
                     QADPDKVKKQP"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            5674..5895
                     /locus_tag="I6L19_25800"
     CDS             5674..5895
                     /locus_tag="I6L19_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXB53038.1"
                     /translation="MTKLGENIPHIIDAAVDFMASSQAFREYLKKLPPDNTIPPDLTDE
                     HVPLYIRRLEYYRLLYRPKQTESYWKKR"
                     /name="hypothetical protein"
     Typing          complement(716..1650)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.023"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagtgcgctt ccttctaatt ttacgaatta gcacggaaga taccccctgc acccgccgac
       61 cgcaagcgat atactgctcg accacaacac accgcaaaaa aacaccaggg agcaccacga
      121 acatgcgcat cagcaacatc gaatggctga agaaacggct cggattcatc aggaagctcg
      181 gagaacagac cgcgcgacag agacagatta ttgacctgct cgacaacgaa gccggactca
      241 cagaacagga acgcaaactt ctccacgtgc tggcaacagc ggaaaaaaac gacttacagg
      301 cacaggagaa cgagcgaaaa cagacgatcc agaaacgaat cgagggaaaa aaacaacgac
      361 gggaacgcaa tcaccggctc tttctcgcgg ctgggctact gatcgaagca ggactggtag
      421 acacaaaaac aggcgagcta tgctataaaa aagacaggat attacaggca cttaaggaaa
      481 ttaagtacga tctggagaca tccccaaacc cagacgcgta agtgcctcca tccgtgttcc
      541 ggttccctgg tgcttcctcg ctcattcgct cgctacgctc ggtcatgaga ctgcggcgag
      601 cactaccagc acaatcatgc ggaaagcagc accattgcag ggctgaacgg ggaaaaacgg
      661 caaaaaacct actgctcaaa aaaacgacag aaaggcgctg ttatctgata taggctattt
      721 tccgcttttc cagtttcgaa tggttcgagt agaaacctcc aacatcgccg caatagcttt
      781 ctgagaatat ccattctcca gcatacatag cgccatgaaa cgcttgtcat cgtaggcttc
      841 gcctttagcg atgccacctt tcctgcctct cttggcctgt atttctggcg tgtgcgtatc
      901 agccacatac tgtgcgaaag tttccggcgt gaagttcctg tgcgtgtatt tcgcaatact
      961 cttgccaata gcccgacatt caggcggtga aagcggaacg ggaagcgatg cgttgtacat
     1021 ttcaacacgc tgaatcacgg catcaagcca ctgtgagaat gctggccagc cctgccgaat
     1081 cgccctgtaa gcccatttgc gcgtcatttc gaacagatgg cagtttctcc ccattccgta
     1141 atgtttatcg atgctacggc gcgctgaggc gctcaaatcg agataatcag ccagttcatc
     1201 gagggagtag ggttcctcgc gccactcctt cacctgccat tccaggtgga acggattttt
     1261 gcaaatcagc ccgctgtagt tcgcgtctgc gtcaagtttc tcacacaacg agcgttcaat
     1321 tgcggcagcg tatttcagtg ccttaaccga actgtccggt gcggttctca cggcgatatt
     1381 gagtgcgtaa agcaggtggg cgtgcccgtt cacaggattt ttaacggtga cgttcggtgc
     1441 gggtgagttc cggtcgctcc agtcgatcgc tgcccccacg cggtcaacgt caaaaaccag
     1501 ccagaactgc gcatgaggct ggttctgctg gatgtaccga gcaagaagcg cacgcccttt
     1561 gccggagatg cgaagaccaa aagcgagatc atccgtgtga tagggacggt ggggtaaatt
     1621 ttcgtcgaag tattgaagcg cggcgctcat gtgttacgat tccttccagg ttatcgaccg
     1681 acctcgcccc tgtattctcg tgacaatctt ggcggagaga cacgaatttc aggggcgttt
     1741 gtttttcagt cgcttacact acacccaacg gcaaatcctg tcgaaccttc ccgaaaaccc
     1801 gatccattta ccataactcc cgttgtacgt accccttaaa acgccctcag tcgctttctg
     1861 gcgcaaaaaa cgcaaaaaga caagaaaaca ccaggccaaa ccagaaaaac gcgtcagaat
     1921 cgttttcagg gcgttgcata actatgcacg aaagcgatgt gcgatcggtt tcgtgcgttc
     1981 tgaacaccgg acggctcaaa acgacaagtt ttcccccccc ggatggaagg ttctgcccgg
     2041 agattttctg tttcagagct tcacgtttcg ccaggacaaa aaaaaccgac ctggggtcgg
     2101 ggcaagaatc agagtctgtg agaaattttt tttagttgtg gaatgcgtca atgttcgcaa
     2161 ccgccccaaa ataccctgag gctgataaaa aacaactggc actatcccgt tccggataaa
     2221 aaaaccacaa aagtggggaa tccaagagaa acgataggtg tcaaattgag tcagaatatt
     2281 gacaatgaaa tagttacgca aataaattta gctgcatcac ttattctgat tcaatactca
     2341 ataaaaaatg cccgcataat aaaccacatt tacaaagata tatatcaaac gaaacaacca
     2401 agttcacagc tcaggataag aatttttctc tatgtaatag ataaaacaca gtttttttat
     2461 aaactgtaat atatgagctg taatattcat gtattaattg tttttttgaa aagcatcaca
     2521 aatttatttt ttacgcatac tgccctattg caaccagcaa tggttttcac ttattttcaa
     2581 catctcattt aatacgagtt atacttcaac ccgtttaaag tgcaacacta actgagtatc
     2641 atttgttttc cttacctacc cctggattcc caggggtttt ttataactac aaaccagcac
     2701 aacaaaaaaa caaaacacaa taaaaacaat gaattatata aaactcaacg atatctttgt
     2761 acggagattt acattcagga acattggggt cagtttggat atagagaatt attgtacggt
     2821 aagcctgttt tttgaacagt ccacattatc gcaatagggt accatccagg gcgttaatta
     2881 tttttgtaga tttctattcc atataatacg ttttcaatga atctggaaga tgaggtgcca
     2941 tgtgtatggc acctcatctc ttattgactc attgattccg taaccgcctg ccaagttata
     3001 aagccaccac tcagattata tgtccggaag ccgtgagact gaagcatacg gtatgccaca
     3061 tgcccccgca gaccactctg acacccgata ataatctctt tatcttcagg caacttatca
     3121 agattctccc gcagggaatc aagagggatg gatagcgcgt caggatattc accaaattgt
     3181 ttcagttccg caggagaacg aatatcaagt aaacaataac tgcccggttc ccgttgtaac
     3241 acatcgcgga catgacagat gaccgtatcg cccttgataa catttgccgc caccattcca
     3301 gcctggttaa cgacgtccct tgcactgttg aaaggcggag cataggtcag ctccagatgc
     3361 tcgagatcgt taacggtaag ccccgcgcgc tgtacgacag ccaggacatc gatgcgttta
     3421 tcaacccctt ttttccctga cgcctgggcc ccgagaattt taccggtatc ggggctgaac
     3481 agcagtttca ggctgatcat tgttgccccc gggtagtaac tggcatgatc agcggcatgg
     3541 acgtaaacct tctcgtatcg ggtaccatgg gctttcagct gcttttcatt cgccccgaca
     3601 ctgccaatac tcagcgaaaa taccttacaa atggatgtcc cctgactgcc atggtaaagg
     3661 ctgtgacgat cgagcatatt atcagcagca atgcgtccct ggcggttggc tggccctgcc
     3721 agaggaacat tagcgggttc ctgaaagaca aggtcaggtg tttcaactgc atcccccacg
     3781 gcatagatgt cagggatact tgtctgcata caggcattga cacttatgcc tccccgttta
     3841 cccacggcga gcccggctcc tgtggcaagg ctgctctcag gttttacccc gatggcgaga
     3901 ataaccatgt cggtctgcag aaacccaccg tcagacagtg caacgcggaa tcccgtctct
     3961 gtccgcagta cctcggtgag cgccgttctg agacgcagat ccacgccatg actcctgatt
     4021 tcctgatgca gggccgatgc catttcagga tcaaccggag ccataacctg ttcccccatc
     4081 tccagcaggg tcacactgat ccccctttcg ctcagtgcct ccatcacttc gagcccgata
     4141 aatccccccc cgacaagcgt ggtgtgagcg atattatgtt gctcaatcca cgcctggata
     4201 gcatccatgt cagtaagatt tcgcagtgta aagacacccg cttcctgtag tccgggcaag
     4261 gggggaacaa ccggagcggc accggtactg aggagcagac gatcccattc ttcggtatag
     4321 acctccccgg aggtcagatt tttaaccgtc accatcttat taaccgggtc tactgacatc
     4381 acttcatgac ggatacggac gtctatgtta aaacgggatt taaaatcctc cggggttttc
     4441 agaatcagcg actgccgtaa cggaattttt ccgcctatat gataaggcaa tccacagttg
     4501 gcgaaagaga catcgcttcc ccgctcaaac acaacaatac tgacatcctc tgataaccga
     4561 cgagccctgg ctgcagcgga tgctcccccg gcaacaccac caacaatcac tattttcatt
     4621 acatcctctc tgttcactcg gggcaaaata tctgccatat ataattagtt acccggtcca
     4681 gctggacgct gcagacctga agatcacggt ggcaaaatta cgccactatc attatactga
     4741 acaatattat ataaaaatac ataatataag aaaccagcaa tgcccatgaa tgaagacaca
     4801 aaacgtcaga ctgatatgaa aaatgcagcc ctgggggcag cagatgtcct acggggactg
     4861 gccaacagcg accggctatt gttgatgtgt cagttaagcc agggggaagc gaatgtgacc
     4921 gagctggaag cggcggtcgg tatcactcag ccgaccctgt ctcagcagtt gggtgtcatg
     4981 cgtcgcctga agctggtcga gacccgacgg gcaggtaaaa tggtatatta ccgtatcggg
     5041 gataaccgca tcatgacgtt gttgaatacc ttatatgatc tctactgccc gcaggcggac
     5101 cccgacaagg tcaaaaaaca accttaacgt acaataattt tcgatatcca aactgccccc
     5161 aacattcacc agcacagtta gacattagca atgcaaaatc aatgcgattc cggtgccgta
     5221 tgtcagggag aattatgtaa acgggggggg ccggaatcgc attccagctc atatcattaa
     5281 atacagaaaa aaacctctga gagcacagag gggataagga atatgaatac tccggtcagg
     5341 atattatcct ggcttttata tacaacatgc tcacaaggac aacaactagc tagttagtta
     5401 atagatatta aaaattttag agctgaaacc ttaaagcttg cttccattcc atgttttatt
     5461 ataaacctta tcttgaaaac tgaaaggaag tacggcagat taattaagtt gcgtgcattc
     5521 ttttcacgtt gtaatacaaa caagaacacc tgcgaagtta tttctcctta catatccccc
     5581 tatgccaacg ggggattttt tctggttcac taacgcataa ataatagtga catactataa
     5641 ttcacgaacc gtgataatat cagatcaatc atcatgacaa aattaggtga aaatatccca
     5701 catattatcg atgcagctgt agattttatg gcatcaagcc aggcatttcg tgagtatctg
     5761 aaaaaacttc cccctgataa cacaataccg ccagatctta ctgatgagca cgtaccgtta
     5821 tatatacgaa ggctggaata ctatcgactg ctttatcgac caaagcagac agaatcatat
     5881 tggaaaaaac ggtgaacggt tcgcgaaatc ctttcgcgct ccgtgaggcg ttggacaaac
     5941 gaagcgcgtc agtttttccg gctcttaccc gtgacaggtt tctccgccag tttatcttcc
     6001 agtgtcacaa ctttctgata caccgccgct gtggcgttgt agttcctgtc gagtattgtt
     6061 ttc
//
