LOCUS       CP077348                6647 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1389 plasmid unnamed6, complete
            sequence.
ACCESSION   CP077348
VERSION     CP077348.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357531
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 725.88x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 17:11:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,169
            CDSs (total)                      :: 5,039
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 329
            CDSs (without protein)            :: 329
            Pseudo Genes (ambiguous residues) :: 0 of 329
            Pseudo Genes (frameshifted)       :: 134 of 329
            Pseudo Genes (incomplete)         :: 190 of 329
            Pseudo Genes (internal stop)      :: 31 of 329
            Pseudo Genes (multiple problems)  :: 23 of 329
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1389"
                     /culture_collection="FDA:FDAARGOS 1389"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            join(5947..6647,1..868)
                     /gene="cea"
                     /locus_tag="I6L19_25720"
     CDS             join(5947..6647,1..868)
                     /gene="cea"
                     /locus_tag="I6L19_25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="QXB53028.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(865..1206)
                     /locus_tag="I6L19_25725"
     CDS             complement(865..1206)
                     /locus_tag="I6L19_25725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="QXB53029.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            1254..1391
                     /locus_tag="I6L19_25730"
     CDS             1254..1391
                     /locus_tag="I6L19_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QXB53030.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(2418..2609)
                     /locus_tag="I6L19_25735"
     CDS             complement(2418..2609)
                     /locus_tag="I6L19_25735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QXB53031.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2673..2996
                     /locus_tag="I6L19_25740"
     CDS             2673..2996
                     /locus_tag="I6L19_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QXB53032.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2938..3696
                     /locus_tag="I6L19_25745"
                     /pseudo=""
     CDS             2938..3696
                     /locus_tag="I6L19_25745"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3674..4192
                     /locus_tag="I6L19_25750"
     CDS             3674..4192
                     /locus_tag="I6L19_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeB"
                     /protein_id="QXB53024.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="mobilization protein MbeB"
     gene            4199..4432
                     /locus_tag="I6L19_25755"
     CDS             4199..4432
                     /locus_tag="I6L19_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QXB53025.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4750..5169)
                     /locus_tag="I6L19_25760"
     CDS             complement(4750..5169)
                     /locus_tag="I6L19_25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QXB53026.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(5250..5687)
                     /locus_tag="I6L19_25765"
     CDS             complement(5250..5687)
                     /locus_tag="I6L19_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QXB53027.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSKGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     Typing          2246..2333
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(1677..2232)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.699"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2987..4536
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggaaaacga aatgaactgg ctcaggcatc cgctaaatat aaagaactgg atgagctggt
       61 caaaaaacta tcaccaagag ccaatgatcc gcttcagaac cgtccttttt ttgaagcaac
      121 cagacgacgg gttggggccg gtaagattag agaagaaaaa caaaaacagg taacagcatc
      181 agaaacacgt attaaccgga taaatgctga tataactcag atccagaagg ctatttctca
      241 ggtcagtaat aatcgtaatg ccggtatcgc tcgtgttcat gaagctgaag aaaatttgaa
      301 aaaagcacag aataatctcc ttaattcaca gattaaggat gctgttgatg caacagttag
      361 cttttatcaa acgctgactg aaaaatatgg tgaaaaatat tcgaaaatgg cacaggaact
      421 tgctgataag tctaaaggta agaaaatcgg caatgtgaat gaagctctcg ctgcttttga
      481 aaaatacaag gatgttttaa ataagaaatt cagcaaagcc gatcgtgatg ctatttttaa
      541 tgcgttggca tcggtgaagt atgatgactg ggctaaacat ttagatcagt ttgccaagta
      601 cttgaagatt acggggcatg tttcttttgg atatgatgtg gtatctgata tcctaaaaat
      661 taaggataca ggtgactgga agccactatt tcttacatta gagaagaaag ctgcagatgc
      721 aggggtgagt tatgttgttg ctttactttt tagcttgctt gctggaacta cattaggtat
      781 ttggggtatt gctattgtta caggaattct atgctcctat attgataaga ataaacttaa
      841 tactataaat gaggtgttag ggatttaatt attctttatt gatataaaaa gtcctagcaa
      901 tccaaatggg attgctagga ccaaacaaag tagattatat agcataaata ggtttaattt
      961 tgctacgggg gcgttattta ggttttttct tctttcgaaa aaatctttct ttatgaagtt
     1021 aaaagctatg tattcaatag catattttga atatggacat agaatagtgc ttatcactat
     1081 tgcatatagc atcttatctg acacaaggaa ataataccct tcgctgtttt ttgttataag
     1141 gtatatatat ataagtgtgc agtacaggcc aaataaaata ttttttatgt agtatcttaa
     1201 gctcataaat taaacctcgc catatattct tttcatttta taaggatcga gttatgagga
     1261 aaagattttt tgtgggaata ttcgcgataa acctccttgt tggatgtcag gctaactata
     1321 tacgtgatgt tcagggaggg accatcgcac catcctcctc ttctaaactg acggggatcg
     1381 cggttcagta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1441 ctgcttgcaa acaaaaaaac caccgctacc aacggtggtt tgtttgccgg atcaagagct
     1501 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     1561 tctagtgtag ccgtagtcgg gccactactt caagaactct gtagcaccgt ttgtgccatc
     1621 atcgctctgc taatccggtt accagtggct gctgccagtg gcgttaaggc gtgccttacc
     1681 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     1741 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata ccaacagcgt
     1801 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1861 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1921 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc tatttttgtg atgctcgtca
     1981 ggggggcgga gcctatggaa aaacgcctgc tacgtggcct tcttcctgtt cctggtcttt
     2041 tgctcacatg ttctttccgg ccttatcccc tgattctgtg gataactgtg ttaccgtttt
     2101 tgtgtgagtc agtaccgctc gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga
     2161 ggaagcggaa aagcgcctgg acgtgcattt tctccttacg catctgtgcg gcatttcaca
     2221 cccggcatgg cgtacttttc atacaatccg cactgatgcc gcatggttaa gccagtatac
     2281 actccgctat cgctacgtga ctgggtcagg gctgcgcccc gacacccgct aaaacctgct
     2341 gacgcgccct gacgggcttg tcagctcccg gcatccgctc acagacaagc tgtgaccgtc
     2401 tccgggagct gcatgtgtca gaggttttca ccgtcatccc cgaaacgtgc gaggcagctg
     2461 cggtaaagct catcggcgtg gtcgtgaagc gattcacaaa tatcggcctg ttcatctgcg
     2521 tccagttcgt tgagcttctc cagcagcgtt aatgtctggc ttctgataaa gcgggccatg
     2581 ttaagggcgg ttttttcctg tttagtcact gatgcctccg tgtaaggggg atttctgttc
     2641 atggggtaat gataccgatg aaacgcgaga ggatgctcac aatacgggtt actgatgatg
     2701 aacatgcccg gttacttgaa cgctgtgagg gtaaacaact ggcggtatgg atgcggcggg
     2761 tctgcctggg ggagccggtt gcccgttccg gaaaactgcc gacactggca ccgccgttac
     2821 tgcgtcagct ggccgccatc ggaaataacc tgaatcagac agcccgtaag gtgaacagcg
     2881 ggcagtggtc ttccggtgac cgggttcagg tggtggccgc actgatggcc atcggggatg
     2941 agctgcgccg gctgcgtctg gctgtcaggg aacagggggc gcgggatgat agttaaattt
     3001 catgccaggg gaaaaggtgg tggcagtggt ccggttgatt acctgctggg gagggagcgt
     3061 aaccgcgaag gcgcaacggt gcttcagggt aatccggaag aagtccggga actcatcgat
     3121 gccacgccat ttgcgaagaa atacacgtcc ggtgttctgt cgttcgcgga gaaggagctg
     3181 ccgccgggag gacgtgaaaa agtgatggcg agctttgagc gtgtactgat gcccggtctc
     3241 gaaaaaaatc agtacagcat cctgtgggtg gagcaccagg acaagggacg gcttgagctg
     3301 aattttgtca ttccgaacat ggagctacag accggaaaac gcctccagcc gtactacgac
     3361 cgcgcagaca ggcctagaat tgatgcctgg cagacgctgg tgaatcacca ttacgggctg
     3421 catgacccga acgccccgga gaaccgcagg acgctgacac tccctgataa cctgcctgaa
     3481 acgaaacagg cgcttgctga gggcgtcacg cgaggtatag atgcacttta ccatgccgga
     3541 gagataaaag gccgtcagga tgtgattcag gcgctcactg aggcggggct ggaagtggtc
     3601 agggtgacgc gaagcagtat cagcattgca gatccgaacg gcgggaagaa tatcaggctg
     3661 aaaggagcat tttatgagca atcttttgca gacgggcgcg gagttcgaga aaaagctgaa
     3721 agagagagcc gaatctacag agaaaatgct gaacaacgag ttcaggaggc tcggcgaatc
     3781 tgtaagcgag gctgtgacat caaacgagac gaaaatcaga gacgctatag ccctgttcac
     3841 agcctcgaca gaggaatcgc tggaaaaaca ccgggaaggg gtgaaagagg cgatgatgca
     3901 gcacaggagg gacgtgttaa agctggcagg gaatacgggc atgatgttac tggggatagt
     3961 ctttctcctg tttaccgcga gtggcgggac gctctggtat cttggaggga ggatacaggc
     4021 gaacctggaa gaaatcagga agcaggaaga gacattgcag aaactgaacg cgaagacatg
     4081 gggcgtggag tttgtgcagg acgggaacag gaaattcctt gtccttccgt acgggaaatc
     4141 agcggaggtg attccctttc aggggaaaga gtgggtacat ctgaaggagt gacacagagt
     4201 gacagagctg gaaacacatt tgctgaacgc cttagagcag ctgcaacagg actatatgca
     4261 gcggctgagc gaatgggaga gcgccttcgt ggaattgcag aagatgtttt cgcttacgca
     4321 acgggacaac gcgatgctga acgagcgggt catgcagttg agtcagcagg tgcagcactt
     4381 gagcgagcag acagaacgct tgagccagtt atacagcgag aactggagat aagagaggaa
     4441 cggctgatac aggagcgcga acatgtgtta tccctggaac gggagcgtca gccggaaata
     4501 caggaacgca cgctggatgg cccttcgctg ggatggtgaa accatgaaaa atggcagctt
     4561 cagtggatta agtgggggta atgtggcctg taccctctgg ttgcataggt attcatacgg
     4621 ttaaaattta tcaggcgcga tcgcggcagt ttttcgggtg gtttgttgcc atttttacct
     4681 gtctgctgcc gtgatcgcgc tgaacgcgtt ttagcggtgc gtacaattaa gggattatgg
     4741 taaatccact tactgtctgc cctcgtagcc atcgagataa accgcagaaa tcgtgtcagc
     4801 cagcagcctg gattttttcg gggtaaggtc tttgctgcgg taatcagtac ccgcaaattg
     4861 agttgtgccc cggagtgagt ttaaaaattc ctggctgtga aactgctgaa tgcgttgttg
     4921 cacatcactc cgtgattttc ctgtcgcttt atctttagct cccatatccc agaactgccg
     4981 aaaaaaaggc accatcattc gtgttgtgtc ggctttttgg gtggaaaagt tgggatcaaa
     5041 accgtcatca gaagcatata cgtaatgccg ggtatgttgt tccacgctgc cctttggggt
     5101 acaggctgtg agagctactg ttgctgttat catggcgata agacaaagtt tgtttccggt
     5161 acgtctcatg agtgtctccc taccttaaag tatttattct cggtgagtta ttatccgaag
     5221 actattttat catcagtctc tgacagcgtt cagcgaccga acaacctgga ccagaatccc
     5281 cgtttctttt ccagttccag tgcctgcctg agctgggcta tctcattttg tagctgttcc
     5341 cgttctgctt cctggcgtct gcgatccata tcctgtgcct gtttatcctc aagcatcagc
     5401 gtcaggcatt gtttcagctc attcagctcc cggagaatgc gttctgtctg ctgatcatgt
     5461 ggattttctg catgtccctt actgtgcctt tctggtgtct cattctgctt taattcgccg
     5521 tatgcccgga tcagttcact ggtttcaaac tcccgtcgtc cgtccctgcc ggtacggtaa
     5581 ctgacaaggc ctgcgcgcat atcacggtaa aactgtgaac gcgatctgcc tgtcattttt
     5641 agtgcgtccc ggagtgtgtg ccatgccata aagtgacagt gtcccataga tgtctcatct
     5701 catagtttca gtaaaacata atgaggtctg agaacggtaa tgtttgtgct ggtttttgtg
     5761 gcatcgggcg agaatagcgc gtggtgtgaa agactgtttt tttgatcgtt ttcacaaaaa
     5821 tggaagtcca cagtcttgac agggaaaatg cagcggcgta gcttttatgc tgtatataaa
     5881 accagtggtt atgtgtacag tatttatttt taacttattg ttttaaaagt caaagaggat
     5941 tttataatgg aaaccgcggt agcgtactat aaagatggtg ttccttatga tgataaggga
     6001 caggtaatta ttactctttt gaatggtact cctgacggga gtggctctgg cggcggaggt
     6061 ggaaaaggag gcagtaaaag tgaaagttct gcagctattc atgcaactgc taaatggtct
     6121 actgctcaat taaagaaaac acaggcagag caggctgccc gggcaaaagc tgcagcggaa
     6181 gcacaggcga aagcaaaggc aaacagggat gcgctgactc agcgcctgaa ggatatcgtg
     6241 aatgaggctc ttcgtcacaa tgcctcacgt acgccttcag caacagagct tgctcatgct
     6301 aataatgcag ctatgcaggc ggaagcagag cgtttgcgcc ttgcgaaagc agaagaaaaa
     6361 gcccgtaaag aagcggaagc agcagaaaag gcttttcagg aagcagaaca acgacgtaaa
     6421 gagattgaac gggagaaggc tgaaacagaa cgccagttga aactggctga agctgaagag
     6481 aaacgactgg ctgcattgag tgaagaagct aaagctgttg agatcgccca aaaaaaactt
     6541 tctgctgcac aatctgaagt ggtgaaaatg gatggagaga ttaagactct caattctcgt
     6601 ttaagctcca gtatccatgc ccgtgatgca gaaatgaaaa cgctcgc
//
