LOCUS       CP077345                5716 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1389 plasmid unnamed3, complete
            sequence.
ACCESSION   CP077345
VERSION     CP077345.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357531
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 725.88x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 17:11:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,169
            CDSs (total)                      :: 5,039
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 329
            CDSs (without protein)            :: 329
            Pseudo Genes (ambiguous residues) :: 0 of 329
            Pseudo Genes (frameshifted)       :: 134 of 329
            Pseudo Genes (incomplete)         :: 190 of 329
            Pseudo Genes (internal stop)      :: 31 of 329
            Pseudo Genes (multiple problems)  :: 23 of 329
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1389"
                     /culture_collection="FDA:FDAARGOS 1389"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            join(5054..5716,1..54)
                     /locus_tag="I6L19_25540"
     CDS             join(5054..5716,1..54)
                     /locus_tag="I6L19_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXB52990.1"
                     /translation="MQLDEHSRKIAALVAYNDKQIKEMNRLITLLNERVNDLNTFTSTL
                     EREGKVVSGISYSFRDSVSNVKFYINKGMDEASCKLQKSVSDTLTSLLREKLSFTLNDV
                     KKEWVDFEEQTSKEIKESLKQTTREAEVATGIIRKTMEKYTVTRIVEKVIVLIVLFLLL
                     MIAPVFYAEYLKMNISSLMEEQKKHESMLKEWKEKAPLAEFSKCGDYRKACVLVDDESG
                     YYFNEQSPNKKYAILK"
                     /name="hypothetical protein"
     gene            complement(112..684)
                     /locus_tag="I6L19_25545"
     CDS             complement(112..684)
                     /locus_tag="I6L19_25545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="QXB52991.1"
                     /translation="MEYSTLLSFAIVTLSQTISIGPGVALVINNAFSHGLKSSIKTSIY
                     IRIGETIVMAISLFALSSTSSTEQHFHIIKIFGGGYLIYIGLMGLINKKNKQRKEQKPF
                     LIPLLNPKAYLFFAALIPTFIDNNTNITLNFFILGVLFIFISFLTDLIYIAISLTIRDK
                     LTPSFSRYISICSSIFILGTGIYFIFT"
                     /name="LysE family translocator"
     gene            complement(691..1641)
                     /locus_tag="I6L19_25550"
     CDS             complement(691..1641)
                     /locus_tag="I6L19_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXB52992.1"
                     /translation="MNNFINECISAPRINGMAEFFSSTASGEKHKPFRLVEIDDRLFHS
                     YDYIKFAKHLTKNSGPFVNHYFCSIPYSKEEECRLGTTVIKYAITLNKKLNTWCLGMAE
                     GAMARTISELSKGNIQTLTTSPTKENEHAFFKYGSSENAYFICTPFFLLEERLKNSNLK
                     QFNQGFDIIIEDTTFQMYSPNRSGQINHVKRLLNNDGIFIFTEKHSSEHDEYQKREIQK
                     DYSFKQRYFSKDEINTKKEIVLNTMNLNEVSVDDMLSAIKQHFKYASIYWNSGNFYSIA
                     ASNSLKHISTFINLLPKAAIPMEYVYEKLPRNISW"
                     /name="hypothetical protein"
     gene            2194..3123
                     /locus_tag="I6L19_25555"
     CDS             2194..3123
                     /locus_tag="I6L19_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QXB52993.1"
                     /translation="MSAVLQLFREKLPHKPYCTNDFTYGVRILPKDIAILARFIQQNQP
                     HAQHWLAFDVDRTGAAIDWSDRNCPAPNITVKNPRNGHAHLLYALTLPVRTAPDASASA
                     LKYAAVIERALCEKLGADVNYSGLICKNPFHQEWQVVEWRWELYTLDELADYLDLSASA
                     RRRVDKDYGMGRNWYLFEKGRKWSYRAIRQGWPAFSPWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTYRKFSPEGFSAVQAARGRKGGNANSSENQAIKGQKSKRVAVATSA
                     RTLKPWVTLGISRATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     gene            complement(3330..3776)
                     /locus_tag="I6L19_25560"
     CDS             complement(3330..3776)
                     /locus_tag="I6L19_25560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="QXB52988.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKTL
                     SALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVDSK
                     TGKPKFSAAELVGALAGIAELPRNHPKWQEWEKRGKELLTKDSA"
                     /name="conjugal transfer protein TraD"
     gene            3948..5033
                     /locus_tag="I6L19_25565"
     CDS             3948..5033
                     /locus_tag="I6L19_25565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QXB52989.1"
                     /translation="MASYHCTVKTGAKGVALKHADYISRSGEYKNYKSREDLEFSSSGN
                     MPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGDRH
                     AYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKKARF
                     GETPQERKSALVALRSHWADVQNEHLARHGHQSRVDHRSLKEQGIKRTPEVHLGPVRVA
                     VMNADQVCAILARRNAEREKLEAEQERDSIIDLKTTLKEALAERDNIPPSLDELGAELK
                     AFVESSFDELNEVMQLNSLINGALDSMMKQFQVEEERKLQQERLIEAEKQRDLERERER
                     VRQRAYSSPAPSRSRDYGMSR"
                     /name="MobA/MobL family protein"
     Typing          2195..3123
                     /name="rep_cluster_2141"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.505"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3949..5030
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggatattatt ttaatgaaca atcaccaaat aaaaaatatg ccatactgaa atagtgttaa
       61 gtaactcatg ccgtcttatg actaaggctg catgagtata aaaacggcgt ttcacgtaaa
      121 aataaaataa atacctgtac ctaatataaa aatggaacta catatgctta tgtatcttga
      181 gaatgatggc gtcaatttat cccttatcgt taagctaata gctatgtaaa taagatctgt
      241 taggaatgat ataaatataa ataaaacacc caatataaaa aagtttaatg tgatatttgt
      301 attattatct ataaaggttg gtattaacgc agcaaagaaa agatacgcct tggggtttaa
      361 taatggtatc aaaaaaggtt tttgctcttt tctttgtttg ttttttttat taattaaacc
      421 cattaatcct atgtatatta aatagcctcc accgaatatt tttataatgt gaaaatgttg
      481 ttctgtgctg gatgttgagg ataacgcgaa aagagatatt gccatgacga tagtctcgcc
      541 aattcttatg tatatacttg tttttattga tgactttaat ccatgtgaaa aggcattgtt
      601 tataaccaat gcaacgccag gccctattga tatagtttgc gataaggtta cgatagcaaa
      661 tgataataat gttgaatatt ccatgtttac ttaccatgat atatttcgtg ggagcttttc
      721 atatacatat tccataggaa ttgcagcttt cggtaataaa tttataaatg ttgatatgtg
      781 ttttagtgaa ttgctggcgg cgattgagta aaaatttccg ctattccaat atattgatgc
      841 gtatttaaaa tgttgtttaa ttgcggaaag catatcgtca acactaactt cattcaaatt
      901 cattgtgttt agtacgattt cctttttggt gttaatctca tctttcgaga aatacctttg
      961 tttgaatgaa taatcttttt ggatttctct tttttgatat tcatcatgtt ctgaactatg
     1021 tttttctgta aatatgaaaa taccatcatt atttaatagc ctttttacgt ggtttatttg
     1081 cccagatctg ttaggtgagt acatttgaaa tgtggtgtct tctattataa tatcgaatcc
     1141 ttgattaaat tgtttaagat ttgaattttt tagtctttcc tctaacagga aaaatggagt
     1201 gcaaataaaa taagcgtttt cacttgagcc atacttaaaa aatgcatgtt cattttcttt
     1261 tgttggactg gttgttaatg tttgtatgtt tccttttgat aattctgaaa tggttcttgc
     1321 cattgcgccc tcagccatac caagacacca tgtgtttaat tttttgttta gcgtgattgc
     1381 gtattttatt actgttgttc ctaatctgca ctcttcttcc ttgctgtatg gtatactgca
     1441 aaaataatga ttaacgaaag ggccgctgtt ttttgtgagg tgtttggcaa acttaatgta
     1501 atcataagaa tgaaaaagcc gatcatctat ttctactagt ctaaaaggtt tgtgtttttc
     1561 ccctgatgca gttgatgaga aaaattctgc catgccgttt attctcggag cggaaataca
     1621 ttcatttata aaattattca ttttcctatc ctctggtttg atttttggaa tttgttgtta
     1681 tgactcttca atgctaattc tcttgtaagc ttttttacac gccataaaca cgattatgat
     1741 gttttatatt gtcgatattt cgttgtccag actggatatg tttggaaatt tgttttcatg
     1801 cgggttagtt cagctggctt tctggatgag aaagtttgct gttttgacca gttcgcgctt
     1861 ctgaaggggt gaaaccgatc attaatcgct tttgtgcata gttatgcagg cccctaaaaa
     1921 cgattctgac gcgttttttg tgttttccct aggtttatct tgtctttttg agttttttgc
     1981 gtcagaaagc gattgagagc gttttagagg gtacgtacaa gaggagttat ggtaaatggg
     2041 tctggttttc gggaaggatc gacaggattt gccgttgggt gtagtgtaac caactgaaaa
     2101 acaaacgccc ctgaaattcg tgccgatccg ccaagactgt cacgaaaatt caaggggcgt
     2161 gtgggtcgta acacacggag gaaatcctaa cacatgagcg ccgtacttca actcttcagg
     2221 gaaaaattac cgcacaaacc gtactgtacg aacgatttca cgtacggcgt tcgcattcta
     2281 ccgaaagata tcgccatcct tgcccgtttc atccagcaga accagccaca tgcgcagcac
     2341 tggctggcct ttgacgttga ccggacgggg gcagcgatcg actggagcga ccggaactgt
     2401 ccggccccga acatcaccgt aaaaaatcca cgtaacgggc acgcgcatct gctctacgcg
     2461 ctcactcttc cggtgagaac cgcgccggat gcatcggctt cggcgctcaa atacgctgcc
     2521 gtgattgagc gtgcgctgtg tgaaaaactg ggcgcggatg tgaactacag cggcctgatc
     2581 tgcaaaaatc cgttccacca ggaatggcag gtggtggaat ggcgttggga actctatacc
     2641 ctcgacgaac tggcggatta tcttgatttg agcgcctcag cgcgccgtcg cgtcgataaa
     2701 gattacggga tggggcgaaa ctggtatctg ttcgaaaagg gccgtaaatg gtcttacagg
     2761 gcgattcgtc agggctggcc agcattctca ccgtggcttg atgcagtgat tcagcgtgtc
     2821 gaaatgtaca acgcatcgct tcccgttccg ctttctccgg ctgaatgtcg ggctattggc
     2881 aagagtattg cgaaatacac ctaccgcaaa ttctctcctg aaggcttttc cgcggtacag
     2941 gctgctcgag gtcgtaaggg cggtaatgca aattcatcag aaaatcaggc tattaaaggt
     3001 cagaaatcaa agcgtgtagc cgttgccaca tcggcacgaa ctctgaagcc gtgggtgacg
     3061 ttagggatca gtcgtgccac atactatcga aaactaaaat gtgatccaga tctcgcaaaa
     3121 tgagaccaga taacaagcct tatcagataa cagcgcctgt aggcgttttt ttgagcgggt
     3181 aggtttgggg tgatttcccg tgtttcgttt ggcaatggtt ttagcgtctg tgtgtgagtg
     3241 gtggttgtgc tcgccgcagt ctcaatgacc gagcgtagcg agcgaatgag cgaggaagca
     3301 ccagggaacc ggaacgtgaa cggaggctct tacgcactgt ctttggtgag tagctctttc
     3361 ccgcgttttt cccattcctg ccattttgga tggttgcgcg gtagctctgc gattcctgcg
     3421 agtgcgccta ccagttcggc agcactaaac tttggttttc cggttttgct gtccaccagt
     3481 cctgccacaa tcatgaggcc tgcggccttg tagagttcat gatcccgcgc ctttcgttcc
     3541 gcccttgcgg ctgccttccg ttctgccgct attattgccg ttaccctggc ttttgctgcg
     3601 gctgctttct ctgcggtttt ttctgcccgt atcagtgcgg acaacgtttt ttcatccgtc
     3661 gttgttcggt tttttttctc tgccagtaac acaagtaatc tttgctgctc tgacggtgat
     3721 ttcagtccgt tgatataggc aatttttgca cttaaccact cttcacgatt tgtcattctt
     3781 acctctgcac ttttgccgtt aatgtgagtt gttattgtgc ataatatcac cttgtctgcc
     3841 tgcgtgtgag accttaaaat catccctgat taattcaggg atgcgcactt agacgtttct
     3901 ctccttcgtc gagaaacagt gcgctcctgc ggagggctta acggcagatg gcaagctacc
     3961 attgtacagt taaaactgga gcaaaaggcg tagccttaaa acatgctgat tatatttcta
     4021 gatccggcga atataagaac tacaaatcgc gggaggatct ggaattttcg tcatccggta
     4081 acatgccatc atgggcgaag aaaaatcccg cagagctatg gaaagcagca gatgaatttg
     4141 aacgaaaaaa cgggacggca taccgtgaaa ttgagatcgc attaccgcga gaactgacac
     4201 gcgaacaacg gatagaactt gttgaagatt tcgtgcaaaa ggagcttggt gatcgccatg
     4261 cctatcagta cgcgatccac aatccgccag gtgcaatcga cggaaaggaa caaccacacg
     4321 cacatatcat gttttgcgag cggattaatg atggtattga acgcgatccg cagcagtttt
     4381 ttaaacgtgc caacagcaaa tcaccggagc ggggaggtgc aaaaaaggca cgctttggtg
     4441 agacaccaca agagcgaaaa tcagccttgg ttgcgctacg ttctcactgg gctgatgtgc
     4501 agaatgaaca ccttgcgcgg cacggccatc agtcgcgcgt tgatcatcgt tcactgaaag
     4561 aacagggtat caagcgcaca ccggaagttc atcttggtcc cgttcgtgtt gctgttatga
     4621 atgctgatca ggtttgtgca attctggcac ggagaaacgc ggaacgggag aagctagaag
     4681 cagagcagga acgggacagc atcattgacc ttaaaacgac cttgaaggaa gcgttagctg
     4741 aacgggataa cataccacca agcctggatg agcttggggc agaattgaag gcgtttgttg
     4801 aaagctcgtt tgatgaactc aatgaggtaa tgcagcttaa ttcattgatt aacggtgcgc
     4861 tggactcaat gatgaagcaa ttccaggtgg aagaggagcg gaagttgcaa caggaacgac
     4921 taattgaagc cgaaaagcaa agagacctgg agagggaaag agaaagagtg cgccagcgtg
     4981 cgtattcctc accagcacca tcacgttcac gtgattatgg tatgagccgt taagcactgg
     5041 aggtttttat tttatgcaat tggatgaaca ttcaagaaaa attgctgctc ttgttgcgta
     5101 taatgataaa caaataaaag aaatgaacag gctaattact ctgcttaatg aaagagtgaa
     5161 tgacttaaat acctttacaa gtactcttga acgtgaaggt aaagttgttt ctggaatttc
     5221 ttattccttt agggatagtg tgtctaatgt taaattttat attaataaag gaatggatga
     5281 ggcatcttgt aaattacaga aatcagtctc agatacacta acgtcattac tccgcgaaaa
     5341 attatctttc actctcaatg atgtaaagaa agagtgggtt gatttcgagg agcaaacaag
     5401 taaagagata aaggaatcat taaagcagac gaccagagag gccgaagttg cgacaggtat
     5461 aattagaaaa acaatggaga agtatacagt aacaaggatt gtcgaaaagg taattgtgtt
     5521 gattgtatta tttttattgc taatgatcgc tcctgttttc tatgctgaat atctcaaaat
     5581 gaatattagt tcgctaatgg aagaacagaa aaaacatgaa tccatgttaa aagaatggaa
     5641 agaaaaagct ccattggccg agttctcgaa gtgtggagat tatcgtaaag catgcgtgtt
     5701 agttgatgac gaaagc
//
