LOCUS       CP077344                4082 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1389 plasmid unnamed2, complete
            sequence.
ACCESSION   CP077344
VERSION     CP077344.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357531
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 725.88x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 17:11:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,169
            CDSs (total)                      :: 5,039
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 329
            CDSs (without protein)            :: 329
            Pseudo Genes (ambiguous residues) :: 0 of 329
            Pseudo Genes (frameshifted)       :: 134 of 329
            Pseudo Genes (incomplete)         :: 190 of 329
            Pseudo Genes (internal stop)      :: 31 of 329
            Pseudo Genes (multiple problems)  :: 23 of 329
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1389"
                     /culture_collection="FDA:FDAARGOS 1389"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            complement(join(3206..4082,1..1283))
                     /locus_tag="I6L19_25525"
     CDS             complement(join(3206..4082,1..1283))
                     /locus_tag="I6L19_25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QXB52986.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            1462..1716
                     /locus_tag="I6L19_25530"
     CDS             1462..1716
                     /locus_tag="I6L19_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QXB52987.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     gene            2249..2950
                     /locus_tag="I6L19_25535"
     CDS             2249..2950
                     /locus_tag="I6L19_25535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QXB52985.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(926..1284)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1285..1461
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2250..2950
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..1370)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.781"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3210..4081)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttcctgagct gttccgctcg tcgctccgcg cgactacgtt ctgtatcctc ctgctccttc
       61 cggcgcaatt cctcttcacg tttaagcaac tggactctga gagctgcaag gccggatgcc
      121 ccccactgct gccactgttt ttcaaatgcc gtctgactcg cgggaatgtc ctgtttctcc
      181 accttccggt aatgctgtcg caatttttcc atttgctggc tcagcgactg gatttttccc
      241 tgttcgctac ggtattccgg tgccgcccat tgcatcaggg gaatacggga aaaaccggac
      301 gaaaacggcg atgatttttt caggctggca gcatggctca tcgcttcctt tcgttcggtg
      361 ttcagctctt cccagcgtct gcgcgtcgcc tgcatatctg ccatcatcga catggcatta
      421 ctggtgagtt tccatgccgc attactgtcc ttcggcaacg cttcgatgat gtcacgtagt
      481 gtgtacgaag ttgcgttgtc aggaagcacg atattcagaa ggtcagccac acggcgggca
      541 ggctctgtca gcccgctgtt tctgagttcg gtacgggaaa tctgtatttc caggttaagt
      601 ctgttaatca gacgattctg ttcgccgcgt tctgtttcga taccgcgtgt ttccatcgca
      661 caggcagact ttccgaggtg aataccaggc gttttctcaa gcccctgtcg ctcgtatgaa
      721 cgatggtcta tctcttcctg ataaccggcg ttcgccagtg ccctgttcgc atggtcagcc
      781 catgatgcac gccacgtctc ggcatgtgtc cggtcgttcc agtccctgac ctttccaccg
      841 aatccagcag gtccgacagc tctcgtggtc agcatgatgt gcgcatgggg atttgtttta
      901 tccatgtgat ggaacgccac atcagcaatc atgccctgac tgacaaagtt ttctctgaca
      961 aaggcgagaa cggtttcccg tgcggcatcc tgcggtagtt cgcgggggat ggcgagttca
     1021 atttcccgcg caagttgtga gttcttccgg cgttcggcct tctcaacggc attccagagt
     1081 tcggcacggt tcgtacacca ggcaggggct gtcgcgggag tcagaatcac agcttcagcc
     1141 acaccgcttt tgcgggtgta gtcgtgagtc agtccggtac gctcatcaag catcagtgaa
     1201 ccggaacgat aggcagcaga agcaacggcg ctgtagccgc tgtttctgga gatgattttc
     1261 atgctgagat gataaatcgc catttctgcc gtaatgcctt tttgtggttt tctttcagcc
     1321 ccgcagggcg cacacaatcg aaacggagtg gagattgtat aagtgcgcac ttcgtgattt
     1381 tccgtgcatt atgccagctc agtttttaaa ctaccagtga cgcgctaaaa tgcgattttt
     1441 catcgcgcac ggagttacat catgagcaga acactcgaac agaagattgc tgatgctgag
     1501 gccagattac agcgcctgaa ggcgaagagc aggagtctgg acacagcgca gaaggtcatt
     1561 gtgggtgcag cattgctggc aaaggtcaga aagccggagg aggtgcagtt gcgtgcctgg
     1621 ttactccagt tcctgaaggc agaggtgaca cgacaggctg atgtgacgcg catactgccg
     1681 ctgattaacg agctggaagc gctgccagga cagtgattgt atcgtccgct cgccgcagtc
     1741 ccagcgaccg agcgttagcg agcgagtggg cgaggaagcg gaagtgaatc cgtcgcctga
     1801 ttgagcacat acgcacggtt tttcgggagg tgatatgcgc tgaacccgca tgaatactgg
     1861 atttccacag ggttggaggg ggtgtattcg gaacttgcaa ttctgctttt ttgcaacgca
     1921 ttgtttttat tggttttttg ttttcagcac tccaagttat ccacaggctc gtcgttaagt
     1981 ttttagatct tgtttttaag atcttgtttt taatacttgt tttagcgcca aaattcctta
     2041 tagaaaacaa aatgttacaa cgctataggt aatatgattt aaggtaaagg taatatgatt
     2101 taaggtgttt aggtggagga atttaaggta aaggcaatat gatttaaggt aatattcaca
     2161 gaaaaaggga tattaaaata aaaaggcatt gcctttcgtt gagggtatgt ttatctttta
     2221 ttcattacct tttggggagg gatgctctat gtcggatcta attgcctata aatccaatgc
     2281 gctggttgaa gccagttata aattgaccct acaggaacag cgatttttgt tactttgcat
     2341 cggtcgattg aagtctgggg ctgatgcaga atctccaaaa ttgcaaaaga caatgaccat
     2401 tacagctgcg gaatatttcg attcattccc tgacatgggg cggaaaaatg ccgaggttca
     2461 gttacaggaa gcaattgatc gtttgtggga caggtcaatc attctcaagg atgacgagaa
     2521 acgtgaggag ttccgctgga tccagtatcg ggcacagtac gctaaaggcg aggccagagc
     2581 acaaataaca ttttctgacg cagtaatgcc ttatttgaca caactacaag ggcaatttac
     2641 tagagttgta atcaagaata tatctagatt atcaagaagt tacagtataa ggatatacga
     2701 actcctccag cagttccgca gtactggcga acgtattatc gctctcgatg attttcgctc
     2761 aatgcttgat atagagcata aatatcaaac atataaatca cttaaccagc aattattaag
     2821 accctgcatt gacgaactga acaaaaaaag cgacctggct gtaacggttg agacgatcaa
     2881 aaaggggcgc acggttgtag ccctgcattt tcgcttcaag gaagacaaac aaatcaagat
     2941 gacgatctaa aatcgatttt aaggcaccta aaacgctccc tactgcgttt tttttgtgtt
     3001 agtagtgcga aggtactatt gaagtgaaaa attgcgtcaa aagcgctcta atgcagcgaa
     3061 aaaagaaccc ccattttttg ggggcggaaa aacaaaacta tgcttgcatt atgtgtgtgc
     3121 ttcttgcatt catccctgtc gatgaacatc gaagaacatc gaagaacata acacgatgtt
     3181 aaacaaaaca aaatattgtt cacattcact gtgaccaacc gcgatcatgt ctcctctcgg
     3241 tggcgtgtgt tcgtgcatct tcctcgcgcc gccattcgtc ccaggtacga ttaaaaccca
     3301 gcgagcggaa cagatgatcc ggctgttttt ctggtgtgtt gacggcagca aaaagcggtg
     3361 cctcacgggt tttcagatga aactccataa gttccggcgc aggtgatatg tggctgtctg
     3421 catcgtgatt tacggcaaca ccataccctg cataccgtaa ccccttatcg gtcagtgcac
     3481 tgatgaccag ctggctgtta tcggctgagg gaacggcaaa cgcgggatac agcgtaccat
     3541 taagccggac aacaccgccc agagagtcat ccacgcccat gaccagcaaa agctgaagct
     3601 ggcgggtttc ccggtcgaca gaactcatta ccggcatcag cgtcggtagc agacgtccct
     3661 gcgtaaaatc caccttcctt gctgccagtg atggcatggc tggctggcgg tcttctccaa
     3721 gacagaaaga ctccggccat gtcttcggct ggcggagatt gcgggtatat tgcggccatg
     3781 tctggcaaac ctgcgtcaga agttgctcaa aggtcgcaga ggaataaaca cgttgcagtg
     3841 gtggatgttg accgatttcg cttcggtcac agatataacc actcctgtca cgcattaaat
     3901 caacacgctc aaagattctg gtgcggtatt tctccagctc gttcccccag agcgtgtatt
     3961 ctccgttccg gttgtgaagc agaaggtaac aggtcatttc cccgttacca ggcatggggg
     4021 cctcgccata ccccgtaacg actgcgctca gtacccctga atccataacg gcattgtcat
     4081 tt
//
