LOCUS       CP077330                5631 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1377 plasmid unnamed5, complete
            sequence.
ACCESSION   CP077330
VERSION     CP077330.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357519
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1378.47x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:53:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,229
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,898
            CDSs (with protein)               :: 4,898
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 62 of 206
            Pseudo Genes (incomplete)         :: 137 of 206
            Pseudo Genes (internal stop)      :: 39 of 206
            Pseudo Genes (multiple problems)  :: 26 of 206
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1377"
                     /culture_collection="FDA:FDAARGOS 1377"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            199..729
                     /locus_tag="I6L07_26115"
     CDS             199..729
                     /locus_tag="I6L07_26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QXB70740.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            736..969
                     /locus_tag="I6L07_26120"
     CDS             736..969
                     /locus_tag="I6L07_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QXB70741.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1450..2502
                     /locus_tag="I6L07_26125"
     CDS             1450..2502
                     /locus_tag="I6L07_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXB70742.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            2504..3370
                     /locus_tag="I6L07_26130"
     CDS             2504..3370
                     /locus_tag="I6L07_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="QXB70743.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(4573..4764)
                     /locus_tag="I6L07_26135"
     CDS             complement(4573..4764)
                     /locus_tag="I6L07_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QXB70739.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4829..5152
                     /locus_tag="I6L07_26140"
     CDS             4829..5152
                     /locus_tag="I6L07_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QXB70744.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5094..5612
                     /locus_tag="I6L07_26145"
                     /pseudo=""
     CDS             5094..5612
                     /locus_tag="I6L07_26145"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          4398..4479
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          3754..3941
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          4..1073
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5143..5630
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.387"
                     /coverage="99.0"
                     /evalue="1.61e-101"
ORIGIN
        1 cggaaacgaa acaggcgctt gctgagggcg tcacacgcgg gatagacgcg ctttaccatg
       61 ccggagagat aaaaggccgt caggatgtga ttcaggcgct cacagaggcg gggcttgaag
      121 tggtcagggt gacgcgaagc agtatcagca ttgcagaccc gaacggcggg aggaatatca
      181 gactgaaagg agcgttttat gagcaatctt ttacagacgg gcgcggagtt cgagaaaaag
      241 ctgaaagaga gagccgaatc tacagagaaa atgctgaacg acgagttcag gaagctcgaa
      301 gaatctgtaa acagggaact gacctcaaac gaatctttaa tcaggaacgc tatcaacgac
      361 catacaacag ccctgaaaga actgctggag aggtatcaga aaacgaccgt cgatacgatg
      421 gacgcgcact ggaaaaccgt actgaaaatg agcgtgaaac gctggctgtg gctgattatc
      481 gtctcagtac tgatgtttgc cacgaccggg agtctgctgt ggtaccaggg catgaagata
      541 aacgcgaata tgaatattct ccgcgagcag aaagagagtc tggagaagct gaacgcgaag
      601 acatggggcg tgcgttatca cgaggacagc aacggacgtt ttctggtgtt gccgaagggg
      661 atgaaagccg aaacgaactg gacgaaggac aacggcaaac tgaacgcagt gagactggtg
      721 caggagtgac agagcatgac ggagctggaa agacatttgc tgaacgcatt agagcaactg
      781 caacaggact atatgcagcg gctgaacgaa tgggagaacg ccttcgtgga attgcagaaa
      841 atgttttcgc ttacgcaacg ggacaacgcg atgctgaacg ggcgagtcat gcagttgagt
      901 cagcaggtgc agcacttgag cgagcggaca gaacgcttga gccagttata cagcgagaac
      961 aggagataag ggatgaacgg ctgatgcagg aacgcgaaca tgagttatct ctggagcggg
     1021 agcgtcagcc ggaaatccag gagcggacac tggatggccc ttcgctggga tggtgaaacc
     1081 atgaaaaatg acgtgttcag tggattaagg cgggctaatg tggcctgtag catctggttg
     1141 cataggtatt catgcggtta aaatttatca ggtgcgatcg cggcagtttt tcgggtggtt
     1201 tgttgccatt tttacctgtc tgctgccgtg atcgcaccga acgcgttttg gcggtgcgta
     1261 caatgcgacg ttatggtaaa tggagcggat tttcaggaag gagtgatact aacaactcga
     1321 acactgtgca aataatgatt tttttaaaat atcggtaacg ataataaatt atttcgtttt
     1381 acgccggtaa taaatataat gtagtttcag tatataaaaa aatatatgag gtgtcttgtg
     1441 ggagctttca tgaatcttca acaacgtata aataaactac cacaattatc atccagtttt
     1501 tcatttggaa aagatattga taatattcat tcatttatct ttaacgaaac ttctaaagat
     1561 aaaattgaag atttactaag aaaatgggtg tcaggcaatc agccctgtgt atttggtaaa
     1621 ttggcaagca agaaaattaa agggcttgat ttccatttat ctattgtaaa tagcccccaa
     1681 ctatataatg atgatggtca tttgtttgat tttttgcgta atgaaagagt tcgttttaaa
     1741 gaacgcgcaa gaagaggaga ggtcagtgcg catctcattt attttattca tccacaactt
     1801 gcctttgctc gtccatcaga agagttagtg gacatacaaa aatatatttg ttcgctgcat
     1861 atgccagagt gctatccaat aaaagaggat gttatttata ctgaaagcgt accatttcag
     1921 gataaagatg ggctaaaaat atataaggct ggtgttaatg ttttttactc ttctgctcat
     1981 agaacaagaa atcatgatag aagaatacca gggggaatac ttatttctgt aaatgccccg
     2041 gggcatttta tgaggctggc gatagaaaaa ggtttctata aagatcaaga acaagcgtta
     2101 gcggatattc gtaacatgac tattcagtct gttggtaagg gtgggtattc acatccggaa
     2161 gggatttcta caacatggca ttcagaatct aatttggatc gttttggatg cccagtgcac
     2221 accggtaata gttcttatta cagcggtttt tatcatactg atgtattaat tccaggagaa
     2281 ttaactaagg atgaaagatt gctccatgaa atagaaaata gtgatccaat gatcttcaac
     2341 tggaatgtat tgttttatgt ttcattagaa gagtttccta tagatgatcc ttattatgga
     2401 gagtttattg gtattcctgt agatgatgca tctatgtttt ttaactcctt tcaaccaagg
     2461 aaatttgaaa acaaacccct ttatgagaaa gaggatgatt aatatgatta aaggaatgct
     2521 ttttgctttt ttagcagcgt tctcatgggg ggcagctatc gtaatgagca aaaaaggagt
     2581 tgaagaacta gatgccggag gggtgttttt ctggcagatt tgtagtgcag taatactatc
     2641 ttggatagtt ctttttataa aaagaaaaaa actcccatta aactcaaaag cgatacttgc
     2701 ttatgcgaca ggcgtttttg aaccattctt agcgtatact tttacactct acggacttta
     2761 ttttatttca gcaggaagcg catcaattat tttttcactt gaatcagtgt ttatattggt
     2821 gttgtctgtt cttatttgct cagttaaaat taattccccg caatatttta tattgttcct
     2881 tataggtgca attgttggca gtattttggt agccttgcca gacatcagta atggatcgaa
     2941 taatattgtt ggatattgtc ttgttgttgc cggtgtgtta tcggcagcat tttatgttgt
     3001 tatatcgtct cgccttatta aatcgtttga gcctatcaca ttattgagcg gacagttgac
     3061 attctccctt atactgtcat cgctctttat ttttatatct aaatcatcaa tttatcttcc
     3121 gttggaatcc gtgattattg ttttttcatc tggaatatta caatattttt tagctttttg
     3181 tttttacttg aactccttgc gttggctccc agttcatttg gcagggataa tgttgtactt
     3241 tattccgcta gttgcattat ttttgtcatg gttgtttttg gatgaacaat tcacattctt
     3301 acagggggta ggatttattg taactgtgct aagtgtatat tttcttaaca gaaaatactc
     3361 agaggagtaa cattctgttc aggattgccc cccggcctcc atcgataccg tgccaaatgc
     3421 acgtaaaaaa cgggagcgtt tctggtgtcg gaatgtaacc aagggggtca gaggcgcgct
     3481 aaaacgctct caggggagtc aaatagagac gtttgtaaca aaaagatcaa aggatcctct
     3541 tgagatcctt tttttctgcg cgtaatctgc tgcctgtaaa cgaaaaaacc gccttgcagg
     3601 gcggtttgat cgaaggttaa gtcagcttgc agactgctta accaggtaac tgtcttatgc
     3661 gagacgagtc accaaaattg ttcttctagt gtagccgcac tccggcagcc acttcaagta
     3721 ctctctaaat atctcgcaca tgacttttac caatggctgc tgccagtggc gctttgtggt
     3781 gtcttttccg ggttggaccc aagagaacag ttaccggaga aggcgcagca gtcgggctga
     3841 acggggggtt cctgcataca gcccagcccg gagcgaactg cctaagtcgc cccgagatac
     3901 cagaagacgt gagctatgag aaagcgccac gtgtcccgta agggagaaag gcggaaaagg
     3961 tatccggtaa tcgatatggc gggaacgtgg gggcgcagga gggagcgatt caccggaaac
     4021 ggtgatgatc tttaagccac gtcgggtttc gccaccgctg gcggatttca tggttgagcc
     4081 gcaaggctcc cacagatgca ccgaaaaagt gtctgttttg tgaagtccgt caggggggcg
     4141 gagcctatgg aaaaagcgcc tccggcgcgg cctttcccgg ggtttcgttt tttgcgcaca
     4201 tgttctttcc tggtttatcc ccgggttctg tggataacag ggttagcgtt tttgagaaag
     4261 gagagtccgc tcgccgcagc cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg
     4321 aagagcgcct gaactggcat tttctcctta cgcatctgtg cggcatttca caccggacat
     4381 ggcgcacttt tcatacaatc cgcactgatg ccgaatggtt aagccagtat acactccgct
     4441 atcgctacgt gactgggtca gggcttcgcc ccgacacccc aaaggtcaac ggcctgacgc
     4501 gccctggcgg gcttgtctgc tcccggcatc cgctcacaga caagctgtga ccgtctccgg
     4561 gagctgcatg tgtcagcggt tttcaccgtc atccccgaag cgtgcgaggc aactgcggta
     4621 aagctcatcg gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag
     4681 ttcgttgagc ttctccagca gcgttaatgt ctggcctctg atgaagcggg ccatgttaag
     4741 ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggggttc tgttcatggg
     4801 ggtaatgata ccgatgaaac gcgagaggat gctcacgata cgggttactg atgatgaaca
     4861 tgcccggtta ctggaacgct gtgagggtaa acaactggcg gtatggatgc ggcgggtctg
     4921 cctgggtgag ccggttgccc gttccgggaa gctgccgaca ctggcaccgc cgttactgcg
     4981 tcagctggcc gccatcggga ataaccttaa ccagacagcg aggaaggtga acagcgggca
     5041 gtggtcttcc ggtgaccggg ttcaggtggt ggccgcactg atggccatcg gggatgagct
     5101 gcgccggctg cgtctggcgg tcagggaaca gggggcgcgg gatgatagtt aagtttcatg
     5161 ccaggggaaa aggtggtggc agtggtccgg ttgattacct gctgggaagg gagcgtaacc
     5221 gggaaggggc aacggtgctt caggggaatc cggaagaggt ccgggaactc atcgatgcca
     5281 cgccatttgc gaagaaatac acgtcaggtg ttctgtcgtt cgcggagaag gagctgccgc
     5341 ctggagggcg tgaaaaagtg atggcgagct ttgagcgtgt actgatgccc ggtctcgaga
     5401 aaaatcagta cagcatcctg tgggtggagc accaggacaa gggacggctt gagctgaatt
     5461 ttgtcattcc gaacatggag ctacagaccg gaaaacgcct ccagccgtac tacgaccggg
     5521 cagacaggcc gagaattgat gcctggcaga cgctggtgaa tcaccattac ggtctgcatg
     5581 accccaacgc cctggagaac cgccggacgc tgacccttcc tgataacctg c
//
