LOCUS       CP077329                4249 bp    DNA     linear   BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1377 plasmid unnamed4.
ACCESSION   CP077329
VERSION     CP077329.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357519
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1378.47x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:53:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,229
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,898
            CDSs (with protein)               :: 4,898
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 62 of 206
            Pseudo Genes (incomplete)         :: 137 of 206
            Pseudo Genes (internal stop)      :: 39 of 206
            Pseudo Genes (multiple problems)  :: 26 of 206
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1377"
                     /culture_collection="FDA:FDAARGOS 1377"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            complement(<1..742)
                     /locus_tag="I6L07_26095"
     CDS             complement(<1..742)
                     /locus_tag="I6L07_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QXB70735.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQ"
                     /name="replication initiation protein"
     gene            2478..2687
                     /locus_tag="I6L07_26100"
     CDS             2478..2687
                     /locus_tag="I6L07_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXB70736.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(2753..3286)
                     /locus_tag="I6L07_26105"
     CDS             complement(2753..3286)
                     /locus_tag="I6L07_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXB70737.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3289..4215)
                     /locus_tag="I6L07_26110"
     CDS             complement(3289..4215)
                     /locus_tag="I6L07_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QXB70738.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     Typing          complement(2..742)
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.07"
                     /coverage="80.0"
                     /evalue="0.0"
     Typing          complement(3293..4215)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 actgtgcgaa agtttccggc gtgaagttcc tgtgcgtgta tttcgcaatg ctcttgccaa
       61 tagcccgaca ttcagccagt gaaagcggaa cgggaagcga tgcattgtac atttcgacac
      121 gctggatcac cgcttcaagc cactgtgaga acgctggcca gccctgacga atcgccctgt
      181 aagcccattt gcgcgtcatt tcgaacaggt ggcagtttcg ccccatcccg taatgtttat
      241 cgatgctacg acgctctgag gcgctcaaat cgagataatc agccagttca tcgagggtat
      301 aggcttcctc gcgccactcc atcaccagcc attccaggtg gaacggattt ttgcaaatca
      361 ggccgctgta gttcacatcc gcacaaagtt tctcacacag cgaacgttca accgctgcgg
      421 catatttcag cgccttaacc gaggcatcag gcgcggttct cacggcgatg ttgagtgcgt
      481 agagcaggtg agcgtgtccg ttcacaggat ttttaacggt gatgttcggc gcgggagcgt
      541 tccggtcgct ccagtcaatc gcagccccct cgcggtcaac gtcaaaaacc agccagaact
      601 gcgcatgagg ctggttctgc tgtatgtacc gcgcaagaag cgcacgccct ttgccggaga
      661 tgcgaagacc aaaagcgaga tcatccgtgt gatagggacg atggggtaaa ttttcgtcga
      721 agtattgaag cgcggcgctc atgtgttacg attctctccg ggttatgcga cctacacgcc
      781 ctctgtattt catgttgaat cttgccggat ctggacatga tttcaggggc gtttgttttt
      841 cagtcgctta cactacaccc aacggcaaat cctgtcgaac cttcccgaaa acccgatcca
      901 tttaccataa ctcccgttgt acgcccccct taaaacgccc tcagtcgctt tctggcgcaa
      961 aaaacgcaaa aagacaagaa aacaccaggc caaaccagaa aaacgcgtca gaatcgtttt
     1021 cagggcgttg cataactatg cacgaaagcg atgtgcgatc ggtttcgtgc gttctgaaca
     1081 ccgaacggct caaaacgaca agttttctcc cctggtgcgt ggcgctctga cggggtttcc
     1141 tgtttccaaa cttcacatct cgccaggaca aaaaaaaacc gacctggggt cggtggcaag
     1201 aatcagagtc tatgggaaat attttagtta tcggatgctc aatattcaca aacgcaaaaa
     1261 atgcctcagt gatgccctaa aacaaccttt ccagaaggag tccgcgcttt acgatgcacg
     1321 gaagagataa aaaaccccac aaaagtgggg aaccaagaga aacaacaatt acaggttgtt
     1381 ggttgtgttc gaacaacgat gaagtagcta cgctaaataa taattaccgt acaggcccta
     1441 catattcaac actaaattta agatcctgac gaaggattta taaatgcacg aaaacacata
     1501 gacagacaaa ttcactaaat tcatacacaa aaacaagtgt agttttctaa gtaaattaga
     1561 ttcgacaacc aattagttta tccacatcgc attccactct tgctacacca atcacatttc
     1621 ttgatatgta atagcagaaa aaataaaacc agacaccaat agaccaaaaa acaaacattc
     1681 aacaagaaac attttgaaca atatcacaaa actgtcacaa ccatcatcag actattgaaa
     1741 aaaactggtt gctgttttat aaaagcgact gtaacaacct ccactctata gttccattag
     1801 tttaaagtgt cacacaaact gagcatccat ttgttttcct tacttttgcc cctgtattcc
     1861 aaggggtttt tttataactt aaagattagc acaccacaca aaaacaataa aaaaaacgac
     1921 aagttataca ataaaaccag tactatccat acagctaatt acatataaat actattaaac
     1981 catctggaca ttaaaaataa actcttataa cataaaatta atgcggttcc ggtaccatat
     2041 gtcagggata atgtgcaaat ggaggtatcg gaactgcatt tcagttcatt attaattaat
     2101 gacaaaaaaa acctctgcga gcgcagaggg ggtaaggaat gttcccggtc ataatatgtc
     2161 gatagtgttt atatacatac agaacaagaa caacaaccag ccaactaata aatattttat
     2221 tttttagagc tgaaacctta aagctcactt ccattccata cattgttata aaccttatgt
     2281 tgaaaactga aagtaagtac ggcagactta ttaagttgcg tgcagtcttt tcacgttata
     2341 caaacaagaa cacctgcgaa gttatttctc cttacatatc cccctatgcc aacgggggat
     2401 tttttctggt tcactaacgc ataaataata gtgacaccct atcattcaca aaccgtgata
     2461 atataaggat tatcatcatg gcaaaattag gtgaaaacac cccacatatt atcgatgcta
     2521 ctgtagattt tatggcatca agccaggcat ttcgtgagta tctgaaaaaa cttccccctg
     2581 ataacacaat accgccagct cttactgatg agcacgtacc gttatatata cgaaggctgg
     2641 aatactatcg tctgctttat cgaccaaagc agacagaatc atattgaaaa aaacggtgaa
     2701 cggttcgcga aatcctttcg cgggtcgtga ggcgttggac aagcgaagcg cgtcagtttt
     2761 tccggctctt ccccgtgaca ggtttctcca ccagtttatc ttccagtgtc acaactttct
     2821 gatacaccgc cgctgtggcg ttgtagttcc tgtcgagtat tgttttcatt ccccatccga
     2881 gcacacagaa cataaccaga aaagccactg acaccccacc cagccatttc cagctatacc
     2941 ctttaagggt gttgtattcc tgcacgatat tgctgtgaat tgtcgcaata tccctgttca
     3001 gggcatccgc gtttgccttc atgtttgcga ctgactcttc cagccgctga aaatcctggc
     3061 tgatgcgctc acctatcagt cctgccacgt ccagcttctg cacctcattt ctgacttcat
     3121 ccctgacctg ttttgccaca ccgtcagaaa tatcccgata tccgcgaaga gtttcccttg
     3181 ccacagccag agaatcagca acagctttct gttgctccat tgcggtatgt tgcagatgct
     3241 ccaccgataa caggagcgtg agtaattttt cttctgtgct ggccatgttc agaatcccat
     3301 agaaaatcca cgtgacggtg atttaacccg ttcaggtgca gttcggttct gtctgctcac
     3361 tctgtctctc atgtcaaagc catgctcccg cgcaacatca tgttgcagcc ttctgaccag
     3421 ttcatgttgc ttaaccggag tgtccagcca ttccctgatg tcatgcccga ccgttcgcgc
     3481 ctgttcaaga tggcgggaat agtccggttc agaccgtttt tgcatgatgt catgacgctg
     3541 ggcatcatca agccgtccgg cagcttcaaa acccagatgc acttcgggac gtcgttcaat
     3601 gccctgttct ttcagcgtcc ggcagtcaat gaagcccctt tcaaagccat gctttctcat
     3661 atgttcgttg tgcttcacct cccagcgttt tcgcaattcc cgcaactgct ctttttgctg
     3721 agtgagagtc aggcttccgg aatccttttt cgcacctccc cgctcgggat acctggcgtt
     3781 atagcgccgg aaatactgtt ccggtgtacg ttcaatgcca tcattcacac gctgactcat
     3841 catgatatgc gcatgaggct gttcgccgcc gtcaatgctc gctttcggat tgtgaatggc
     3901 aaaactccag gcgtgccgtt cgccagcttc ctgacgtaca aaatcctgca ccagttcaag
     3961 gcgctgttcc ggtgtcagtt cacggggtaa tgcaatttca agttccctat aggtcgaacc
     4021 gttcgcacgt tcaaactggt cagccgcctg ccagaaatgg gaaggattat ccctggccca
     4081 ttccggcatg tttccgtgtg cggaatattc caaatcctga cggtcgcggt atttctcttt
     4141 gcgctcgatg taatcggcgt ggtttgcagc ctgccccttg ccaccaaatt taacgctgag
     4201 gtgataggtt gccacggttt cagccctctg caggagcgca ctgtttctc
//
