LOCUS       CP077316                5153 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1374 plasmid unnamed2, complete
            sequence.
ACCESSION   CP077316
VERSION     CP077316.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357516
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 889.04x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:45:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,146
            CDSs (total)                      :: 5,023
            Genes (coding)                    :: 4,842
            CDSs (with protein)               :: 4,842
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 85 of 181
            Pseudo Genes (incomplete)         :: 91 of 181
            Pseudo Genes (internal stop)      :: 44 of 181
            Pseudo Genes (multiple problems)  :: 35 of 181
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1374"
                     /culture_collection="FDA:FDAARGOS 1374"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            12..431
                     /locus_tag="I6L04_25700"
     CDS             12..431
                     /locus_tag="I6L04_25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QXA37916.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(656..979)
                     /locus_tag="I6L04_25705"
     CDS             complement(656..979)
                     /locus_tag="I6L04_25705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="QXA37917.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="nikA protein"
     gene            1263..1457
                     /locus_tag="I6L04_25710"
     CDS             1263..1457
                     /locus_tag="I6L04_25710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QXA37918.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2299..2436)
                     /locus_tag="I6L04_25715"
     CDS             complement(2299..2436)
                     /locus_tag="I6L04_25715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QXA37919.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            2484..2825
                     /locus_tag="I6L04_25720"
     CDS             2484..2825
                     /locus_tag="I6L04_25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="QXA37920.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(2822..4387)
                     /locus_tag="I6L04_25725"
     CDS             complement(2822..4387)
                     /locus_tag="I6L04_25725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-10"
                     /protein_id="QXA37914.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCSFIDKNKLNTINE
                     VLGI"
                     /name="colicin-10"
     gene            4647..5084
                     /locus_tag="I6L04_25730"
     CDS             4647..5084
                     /locus_tag="I6L04_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QXA37915.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="entry exclusion protein 1"
     Typing          complement(989..1228)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.6e-123"
     Typing          1487..2016
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.59"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(660..979)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.11e-62"
ORIGIN
        1 gggagacact catgagacgt accggaaaca aactttgtct tatcgccatg ataacagcaa
       61 cagtagctct cacagcctgt accccaaagg gcagcgtgga acaacatacc cggcattacg
      121 tatatgcttc tgatgacggt tttgatccca acttttccac ccaaaaagcc gacacaacac
      181 gaatgatggt gccttttttt cggcagttct gggatatggg agctaaagat aaagcgacag
      241 gaaaatcacg gagtgatgtg caacaacgca ttcaacagtt tcacagccag gaatttttaa
      301 actcactccg gggcacaact caatttgcgg gtactgatta ccgcagcaaa gaccttaccc
      361 cgaaaaaatc caggctgctg gctgacacga tttctgcggt ttatctcgat ggctacgagg
      421 gcagacagta agtggattta ccataatccc ttaattgtac gcaccactga aacgcgctga
      481 acgcgattct gacgcagaca ccgcaaaaag agatgtttta ccccggaaaa acaggaagga
      541 tgcgtcagga tcgttttcag ctctctgcat agctatgcat gaaagcgaat ggtgatcact
      601 ttgggagctt acggtgttca taccgtcagt tttcgacagt tttctctccg ggaagctaat
      661 ctgccataag cctggataac agggcacggt gatactccgt aatagcgatc agcacctccg
      721 catactccct gtccccgaca cgcttgccgt cgataaagag ttttttctgt aacgccccca
      781 gtctcataac ttctttcagt acccgatcat cagtcagtga gttaactttc ttaccgagag
      841 ctgccgcccg taaaaaaccg gaaacggtct tgccgcattc agcagccctt tttctgattt
      901 cctggtcttc ttctgccgtc agacgaacag ggcgactgat agcccgcctg cgcgtattac
      961 ttccacttcg ttttgtcata aaacatggct ccgtatctga catgggtgtc ggggcaaagc
     1021 cctgaccagg gtaattgtaa tagcgtgcat gtatgcgcgg tataacaatt acacatcctg
     1081 tcctgtcagc aagttcgaat taccgacaaa ggcacggcag ttttttggca gtgacagtat
     1141 tgtcaggcag aaatacccgt caaagcgtca gacggcagaa taccggcggt aaaacagtcg
     1201 aaaagtggta cgcgatataa tcacacaacg cagatgacag acagaacgga gaaaacgaaa
     1261 agatgaacaa gcagcagcaa gccgtactca acatggcagg attcataaaa agccaaagcc
     1321 tgacgctgct cgaaaagctg gacgcactcg atgctgacga acaggctacc atgtgtgaga
     1381 agctgcacga actcgcagaa gaactccaga acagcatcca gacacgcttt gaagtagaaa
     1441 atagcacaga gagataacgc tcaggacagg atgccgaacg gcacaacaga gcgtaaggag
     1501 aaaatgtcac ataaggcgct cttccgcttc ctcgctcact gactcgctac gctcggacgt
     1561 tcgactgcgg cgagcggtgt cagctcactc aaaagcggta atacggttat ccacagaatc
     1621 aggggataaa gccggaaaga acatgtgagc aaaaagcaaa gcaccggaag aagccagcgc
     1681 cgcaggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1741 gccagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1801 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1861 cttcgggaag cgtggcgctt tctcatagct cacgctgttg gtatctcggc tcggtgtagg
     1921 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1981 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttagcg ccactggcag
     2041 cagccgttgg taacttggtt agcagagcga tgatggcgca aatggtgcaa cagagttctt
     2101 gaagtagtgg cccgactacg gctacactgg aaggacagta tttggtatct gcgctctgct
     2161 gaagccagtt acctcggtta agcagttccc caactgactt aaccttcgat caaaccgcct
     2221 ccccaggcgg ttttttcgtt tacaggcagc agattacgcg cagaaaaaaa ggatctcaag
     2281 aagatccttt gatcttttct actgaacaga gatccccgtc aatttagaag aagatgatgg
     2341 agccacagtc cctccctgaa catcacgtat atagttagcc tgacatccaa caaggaggtt
     2401 tatagcgaat attcccacaa aaaatctttt cctcataact cgatccttat aaaatgaaaa
     2461 gaatatataa cgaggtttaa tttatgagtt taagatacta cataaaaaat attttgtttg
     2521 gcctatactg cgcacttata tatatatacc ttataacaaa aaacaacgaa gggtattatt
     2581 tcctagcgtc agataagatg ctatacgcaa tagtgataag cactattcta tgcccatatt
     2641 caaaatatgc tattgaacac atatttttta agttcataaa gaaagatttt ttcagaaaaa
     2701 gaaaaaacct aaataatgcc cccgtggcaa aattaaacct atttatgcta tacaatctac
     2761 tttgtttggt cctagcaatc ccatttggat tgctaggact gtttatatca ataaagaata
     2821 attaaatacc caacacctca tttatagtat taagtttatt cttatcaata aaggaacata
     2881 gaatgcctgt aacaatagca atcccccaga tacctaatgt agttccagca agcacactaa
     2941 aaagtaaaac aacaacataa ctaactccag catctacagc tttcttctct aatgtaagaa
     3001 atagcggctt ccagtcacct gtatccttaa tttttaggat atcagatacc acatcatatc
     3061 caaaagaaac atgtcccgta atcttcaagt acttggcaaa ctgatctaaa tgcttagccc
     3121 agtcttcata cttaaccgcc tccagtgcat tgaaaattgc atcacggtct gctttgctga
     3181 atttcttatt taatacatcc ttgtatttct caaaagcagc tagagcttca ttcacattgc
     3241 tgattttctt acctttagat ttatcagcaa gttcctgtgc catttttgaa tatttttcac
     3301 catatttttc agatagcgtt tgataaaagc taactgttgc atccacagca tccttaatct
     3361 gcgagttcag gagattagtc tgtgctgttt tcaaattatc ttcagcatca tgaatacgtg
     3421 aaactgctgt acttcgctta ttattagcct gagaaatagc ctcttggatt ccatttatct
     3481 cagagctaat ctggttaaga cgcgtttctg atgctgttac ctgcttttgt ttttcctcca
     3541 tctcatcacc agctctcgcc cgtagtctgg tggcctcaaa aaaaggacgg ctctgaagcg
     3601 gatcattcgc cctaggagat aaaagtttaa ccctttcatc cagttcttta tatttagcag
     3661 atgcctgatc caactcattt cgttttccgg acagcgtatt cgtttctgca tcacgggcat
     3721 gaatactgga gcttaaacgg gtattgagcg tattaatctc ttcatccact ttcgccagct
     3781 cagattgtgc agcagcaaga tttttttgtg ccacctccac agcccgggcc tcttcactca
     3841 atgctgccag gcgtttctct tcatcctcag ccagtttcaa ttggcgttct gtttcagcct
     3901 gctccttctc tatctcttta cgtcgttgtt ctgcttcctg aaaagccttt tccgcagctt
     3961 ccgcttcttt acgggctttt tcttctgctt ttgcaaggcg caaccgctct gcttctgcct
     4021 gcatcgctgc attattggca tgagccaggt caataacctc cggatgagtg gaattatggc
     4081 gaagcgcctc attcacaata tccttcagat gttgagtcag cgcatcccgg tttgcttttg
     4141 ctttagcctg tgcttctgcg gcagcttttg ctcgggcagc ctgttctgcc tgcgttttct
     4201 tcaattgagc agtagaccat ttagctgtgg catgaatggc tgcagaactt tcacttttgc
     4261 tacctccagt tccaccacca ccgccagagc cactcccgtc tggattacca ttcaaaagag
     4321 taatgattac ctgcccctta tcatcataag gaacaccatc tttatagtac gctacagctg
     4381 tttccattat aaaatcctct ttgacattaa aaacaaatag ttaaaagcaa tatactgtat
     4441 atataatcac tggttttata tacagtttaa aattacgccg ctgcattttc cctgtcaacc
     4501 ctgtggattt tcatttttgt gaaaacgatc aaaaaaacag ccacccacac cacgtgctat
     4561 ttccacccga tgccacaaaa accagcacaa acattaccgt tctcagacct cattatgttt
     4621 tactgaaacc atgagatgag acacctatgg gacactgtaa ctttatggca tggcacacac
     4681 tccgggacgc actgaaaatg actggaagat cgcgttcaca gttttaccgt gatatgcgct
     4741 caggtcttgt cagttaccgt accagccagg acggacgacg ggagtttgaa accagtgaac
     4801 tgatccgggc atacggcgaa ttaaagcgga atgagacacc agaaaggcac actcaaggac
     4861 actcagaaaa acctcatgat cagcagacag aacgcattct ccgggaactg aatgagctga
     4921 aacaatgcct gacgctgatg cttgaggata aacaagccca ggatacggag cgccggcgcc
     4981 aggaagcaga acggaaacag ctacagaatg agatagccca gctcaggcag gctctggagc
     5041 aggaaaagaa acggggattc tggtccaggc tgttcggtca ctgaacgcgc tcagaaactg
     5101 atgataagat aatcttcgga taataactca ccgagaataa atactttaag gta
//
