LOCUS       CP077299                2506 bp    DNA     linear   BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1386 plasmid unnamed5.
ACCESSION   CP077299
VERSION     CP077299.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357528
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1865.33x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:25:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,049
            CDSs (total)                      :: 4,933
            Genes (coding)                    :: 4,776
            CDSs (with protein)               :: 4,776
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 68 of 157
            Pseudo Genes (incomplete)         :: 83 of 157
            Pseudo Genes (internal stop)      :: 36 of 157
            Pseudo Genes (multiple problems)  :: 28 of 157
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1386"
                     /culture_collection="FDA:FDAARGOS 1386"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            complement(487..678)
                     /locus_tag="I6L16_25225"
     CDS             complement(487..678)
                     /locus_tag="I6L16_25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QXB17495.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDSENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            743..1066
                     /locus_tag="I6L16_25230"
     CDS             743..1066
                     /locus_tag="I6L16_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QXB17498.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1008..1766
                     /locus_tag="I6L16_25235"
                     /pseudo=""
     CDS             1008..1766
                     /locus_tag="I6L16_25235"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1744..2262
                     /locus_tag="I6L16_25240"
     CDS             1744..2262
                     /locus_tag="I6L16_25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QXB17496.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSC
                     GTLWYLGGMIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPHQ
                     GKDWVQLME"
                     /name="MbeB family mobilization protein"
     gene            2269..2502
                     /locus_tag="I6L16_25245"
     CDS             2269..2502
                     /locus_tag="I6L16_25245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QXB17497.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          315..402
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          1057..2474
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.389"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 cgggtttcgc cacctctgac ttgagcgtcg gtttttgtga tgctcgtcag gggggcggag
       61 cctatggaaa aacgccatcg gcgtggcctt ttgttgggct ttgccttttt gcgcacatgt
      121 tctttccggt cttatccccg gattctgtgg ataactgtat tatcgttttt gagtgagtca
      181 gtaccgctcg ccgcagccga acgaccgagc gtagcgagtc agtgagcgag gaagcggaaa
      241 agcgccggaa cgtgcatttt ctccttacgc atctgtgcgg tatttcacac tggtcatggt
      301 gcacttttca tacaatccgc actgatgctg catagttaag ccagtataca ctccgctatc
      361 gctacgtgac tggttcaggg ctgcgccccg aaacccgcta aaacaccctg acgcgccctg
      421 gcgggcttgt ctgctcccgg catccgctca caggcaagct gtgaccgtct ccgggagctg
      481 catatgtcag aggttttcac tgtcatcccc gaagcgcgcg aggcagctgc ggtaaagctc
      541 atcggcgtgg tcgtgaagcg attcacagat atctgcctgt tcatccgcgt ccagttcgtt
      601 gagcttctcc agcagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      661 tttttcctgt ttagtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat
      721 gataccgatg aaacgcgaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
      781 gttactggaa cgctgtgagg gtaaacaact ggcggtatgg atgcggcggg tctgcctggg
      841 tgagccggtt gcccgttccg gtaggctgcc gacactggca ccgccgttac tgcgtcagct
      901 ggcagccatc gggaataacc tgaaccagac agcccgtaag gtgaacagcg gacagtggtc
      961 ttccggcgac cgggttcagg tggtggccgc actgatggcc atcggggatg agctgcgccg
     1021 gttgcgtctg gccgtcaggg aacagggggc gcgggatgat agttaagttt catgccaggg
     1081 gaaaaggtgg tggcagtggt ccggttgatt acctgctggg aagggagcgt aaccgggaag
     1141 gggcaacggt gcttcagggt aatccggaag aagtccggga actcatcgat gccacgccat
     1201 ttgcgaagaa atacacgtcc ggtgttctgt cgttcgcgga gaaggagctg ccgccgggag
     1261 gacgtgaaaa agtgatggcg agctttgagc gtgtactgat gcccggtctc gaaaaaaatc
     1321 agtacagcat cctgtgggtg gagcaccagg acaagggacg gcttgagctg aattttgtca
     1381 ttccgaacat ggagctggag accggaaaac gcctacagcc gtactacgac cgggcagaca
     1441 ggccgagaat tgatgcctgg cagacgctgg tgaatcacca ttacgggctg catgacccga
     1501 acgccccgga gaaccgccgg acgctgactc tccctgataa cctgccggaa acgaaacagg
     1561 cgtttgctga gggcgtcaca cgcgggatag atgctcttta ccatgcagga gagataaaag
     1621 gccgtcagga tgtgattcag gcgctcacgg aagcagggat tgaagtggtc agggtgacgc
     1681 gaagcagtat cagcattgca gacccgaacg gcgggaagaa tatcagactg aaaggagcat
     1741 tctatgagca atcttttaca gacgggcgcg gagttcgaga aaaagctgaa agagagagcc
     1801 gaatctacag agacaatgct gaacgacgag ttcagcaagc tcgaaaaatc tgtaagcaag
     1861 gctgtgacat caaacgagac gaaaatcaaa gacgctatag ccctgttcac agcttcgaca
     1921 gaggaatcac tgaaaaagca ccgggaaggg gtgaaagagg cgatgatgca gcacaggaag
     1981 gacgtgttaa agctggcagg gaatacgggc atgatgttac tggggatggt cattttcctg
     2041 tttaccgtga gttgcgggac gctctggtat cttggaggga tgatacaggc gaacctggaa
     2101 gatatcagga agcaggaaga gacattgcag aaactgaacg cgaagacatg gggcgtggag
     2161 ttcgtacagg acgggaacag gaaatttctg gtccttccgt acgggaaatc agcagaggtg
     2221 attccccatc aggggaagga ctgggtacaa ctgatggagt gacacagagt gacggagctg
     2281 gaaagacatt tgctgaacgc attagagcaa ctgcaacagg actatatgca gcggctgaac
     2341 gaatgggaga gcgccttcgt ggaattgcag aaaatgtttt cgcttacgca acgggacaac
     2401 gcgatgctga acgagcgggt catgaagttg agtcagcagg tgcagcactt gagcgagcgg
     2461 acagaacgct tgagccagtt atacagcgag aacaggagat aaggga
//
