LOCUS       CP077298                4535 bp    DNA     linear   BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1386 plasmid unnamed4.
ACCESSION   CP077298
VERSION     CP077298.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357528
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1865.33x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:25:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,049
            CDSs (total)                      :: 4,933
            Genes (coding)                    :: 4,776
            CDSs (with protein)               :: 4,776
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 68 of 157
            Pseudo Genes (incomplete)         :: 83 of 157
            Pseudo Genes (internal stop)      :: 36 of 157
            Pseudo Genes (multiple problems)  :: 28 of 157
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1386"
                     /culture_collection="FDA:FDAARGOS 1386"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            <1..180
                     /locus_tag="I6L16_25195"
     CDS             <1..180
                     /locus_tag="I6L16_25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="QXB17489.1"
                     /translation="ESAGAALERADRTLEPVIQREQEIRDERLIQEREHELSLERERQL
                     EIQERTLDGPSLGW"
                     /name="nuclease"
     gene            complement(635..1054)
                     /locus_tag="I6L16_25200"
     CDS             complement(635..1054)
                     /locus_tag="I6L16_25200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QXB17490.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(1135..1572)
                     /locus_tag="I6L16_25205"
     CDS             complement(1135..1572)
                     /locus_tag="I6L16_25205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QXB17491.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="entry exclusion protein 1"
     gene            1832..3397
                     /locus_tag="I6L16_25210"
     CDS             1832..3397
                     /locus_tag="I6L16_25210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-10"
                     /protein_id="QXB17492.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDTTVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-10"
     gene            complement(3394..3735)
                     /locus_tag="I6L16_25215"
     CDS             complement(3394..3735)
                     /locus_tag="I6L16_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="QXB17493.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            3783..3920
                     /locus_tag="I6L16_25220"
     CDS             3783..3920
                     /locus_tag="I6L16_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QXB17494.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     Typing          complement(4167..4535)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.297"
                     /coverage="86.0"
                     /evalue="1.33e-177"
ORIGIN
        1 gagtcagcag gtgcagcact tgagcgagcg gacagaacgc ttgagccagt tatacagcga
       61 gaacaggaga taagggacga acggctgata caggaacgcg aacatgagtt atccctggag
      121 cgtgagcgcc agctggaaat acaggaacgg acactggatg gcccttcgct gggatggtaa
      181 tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc attttttggg ataatggttg
      241 gttcggggca ccgggaacgc gcttcagtca taaaccttgc tgaaaacaca ggaacgcgtt
      301 aaaacgcata cgggcgcgat tctggtgtgt ctttcatagt cgtgtttttg cctctattgc
      361 cagatcgcgc tcattcccct gaacttctgt tttcctgctc aaatccccgt cttcttcgct
      421 tccgaaagta tgaaaaaaat ccgtttcagc ggtctctgac tggcaattcc tgcgtgattc
      481 acctggctgc ataggtatgc atgtggttaa aatttaccgg gtgcgatcgc ggcagttttc
      541 cgggtggttt gttgccattt ttacctgtct gctgccgtga tcgcaccgaa cgcgtttcgg
      601 cggtgcgtac aataagggat tatggtaaat ccacttactg tctgccctcg tagccatcga
      661 gataaaccgc agaaatcgtg tcagccagca gcctggattt tttcggggta aggtctttgc
      721 tgcggtaatc agtacccgca aattgagttg tgccccggag tgagtttaaa aattcctggc
      781 tgtgaaactg ttgaatgcgt tgttgcacat cactccgtga ttttcctgtc gctttatctt
      841 tagctcccat atcccagaac tgccgaaaaa aaggcaccat cattcgtgtt gtgtcggctt
      901 tttgggtgga aaagttggga tcaaaaccgt catcagaagc atatacgtaa tgccgggtat
      961 gttgttccac gctgcccttt ggggtacagg ctgtgagagc tactgttgct gttatcatgg
     1021 cgataagaca aagtttgttt ccggtacgtc tcatgagtgt ctccctacct taaagtattt
     1081 attctcggtg agttattatc cgaagattat cttatcatca gtttctgagc gcgttcagtg
     1141 accgaacagc ctggaccaga atccccgttt cttttcctgc tccagagcct gcctgagctg
     1201 ggctatctca ttctgtagct gtttccgttc tgcttcctgg cgccggcgct ccgtatcctg
     1261 ggcttgttta tcctcaagca tcagcgtcag gcattgtttc agctcattca gttcccggag
     1321 aatgcgttct gtctgctgat catgaggttt ttctgagtgt ccttgagtgt gcctttctgg
     1381 tgtctcattc cgctttaatt cgccgtatgc ccggatcagt tcactggttt caaactcccg
     1441 tcgtccgtcc tggctggtac ggtaactgac aagacctgag cgcatatcac ggtaaaactg
     1501 tgaacgcgat cttccagtca ttttcagtgc gtcccggagt gtgtgccatg ccataaagtt
     1561 acagtgtccc ataggtgtct catctcatgg tttcagtaaa acataatgag gtctgagaac
     1621 ggtaatgttt gtgctggttt ttgtggcatc gggtggaaat agcacgtggt gtgggtggct
     1681 gtttttttga tcgttttcac aaaaatgaaa atccacaggg ttgacaggga aaatgcagcg
     1741 gcgtaatttt aaactgtata taaaaccagt gattatatat acagtatatt gcttttaact
     1801 atttgttttt aatgtcaaag aggattttat aatggaaaca gctgtagcgt actataaaga
     1861 tggtgttcct tatgatgata aggggcaggt aatcattact cttttgaatg gtaatccaga
     1921 cgggagtggc tctggcggtg gtggtggaac tggaggtagc aaaagtgaaa gttctgcagc
     1981 cattcatgcc acagctaaat ggtctactgc tcaattgaag aaaacgcagg cagaacaggc
     2041 tgcccgagca aaagctgccg cagaagcaca ggctaaagca aaagcaaacc gggatgcgct
     2101 gactcaacat ctgaaggata ttgtgaatga ggcgcttcgc cataattcta ctcatccgga
     2161 ggttattgac ctggctcatg ccaataatgc agcgatgcag gcagaagcag agcggttgcg
     2221 ccttgcaaaa gcagaagaaa aagcccgtaa agaagcggaa gctgcggaaa aggcttttca
     2281 ggaagcagaa caacgacgta aagagataga gaaggagcag gctgaaacag aacgccaatt
     2341 gaaactggct gaggatgaag agaaacgcct ggcagcattg agtgaagagg cccgggctgt
     2401 ggaggtggca caaaaaaatc ttgctgctgc acaatctgag ctggcgaaag tggatgaaga
     2461 gattaatacg ctcaataccc gtttaagctc cagtattcat gcccgtgatg cagaaacgaa
     2521 tacgctgtcc ggaaaacgaa atgagttgga tcaggcatct gctaaatata aagaactgga
     2581 tgaaagggtt aaacttttat ctcctagggc gaatgatccg cttcagagtc gtcctttttt
     2641 tgaggccacc agactacggg cgagagctgg tgatgagatg gaggaaaaac aaaagcaggt
     2701 aacagcatca gaaacgcgtc ttaaccagat tagctctgag ataaatggaa tccaagaggc
     2761 tatttctcag gctaataata agcgaagtac agcagtttca cgtattcatg atgctgaaga
     2821 taatttgaaa acagcacaga ctaatctcct gaactcgcag attaaggatg ctgtggatac
     2881 aacagttagc ttttatcaaa cgctatctga aaaatatggt gaaaaatatt caaaaatggc
     2941 acaggaactt gctgataaat ctaaaggtaa gaaaatcagc aatgtgaatg aagctctagc
     3001 tgcttttgag aaatacaagg atgtattaaa taagaaattc agcaaagcag accgtgatgc
     3061 aattttcaat gcactggagg cggttaagta tgaagactgg gctaagcatt tagatcagtt
     3121 tgccaagtac ttgaagatta cgggacatgt ttcttttgga tatgatgtgg tatctgatat
     3181 cctaaaaatt aaggatacag gtgactggaa gccgctattt cttacattag agaagaaagc
     3241 tgtagatgct ggagttagtt atgttgttgt tttacttttt agtgtgcttg ctggaactac
     3301 attaggtatc tgggggattg ctattgttac aggcattcta tgtgccttta ttgataagaa
     3361 taaacttaat actataaatg aggtgttggg tatttaatta ttctttattg atataaacag
     3421 tcctagcaat ccaaatggga ttgctaggac caaacaaagt agattgtata gcataaatag
     3481 gtttaatttt gccacggggg cattatttag gttttttctt tttctgaaaa aatctttctt
     3541 tatgaactta aaaaatatgt gttcaatagc atattttgaa tatgggcata gaatagtgct
     3601 tatcactatt gcgtatagca tcttatctga cgctaggaaa taataccctt cgttgttttt
     3661 tgttataagg tatatatata taagtgcgca gtataggcca aacaaaatat tttttatgta
     3721 gtatcttaaa ctcataaatt aaacctcgtt atatattctt ttcattttat aaggatcgag
     3781 ttatgaggaa aagatttttt gtgggaatat tcgctataaa cctccttgtt ggatgtcagg
     3841 ctaactatat acgtgatgtt cagggaggga ctgtggctcc atcatcttct tctaaattga
     3901 cggggatctc tgttcagtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3961 cgtaatctgc tgcctgtaaa cgaaaaaacc gcctggggag gcggtttgat cgaaggttaa
     4021 gtcagttggg gaactgctta accgaggtaa ctggcttcag cagagcgcag ataccaaata
     4081 ctgtccttcc agtgtagccg tagtcgggcc actacttcaa gaactctgtt gcaccatttg
     4141 cgccatcatc gctctgctaa ccaagttacc aacggctgct gccagtggcg ctaagtcgtg
     4201 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4261 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgagc cgagatacca
     4321 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4381 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccggggg gaaacgcctg
     4441 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcggt ttttgtgatg
     4501 ctcgtcaggg gggcggagcc tatggaaaaa cgcca
//
