LOCUS       CP077297                5544 bp    DNA     linear   BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1386 plasmid unnamed3.
ACCESSION   CP077297
VERSION     CP077297.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357528
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1865.33x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:25:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,049
            CDSs (total)                      :: 4,933
            Genes (coding)                    :: 4,776
            CDSs (with protein)               :: 4,776
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 68 of 157
            Pseudo Genes (incomplete)         :: 83 of 157
            Pseudo Genes (internal stop)      :: 36 of 157
            Pseudo Genes (multiple problems)  :: 28 of 157
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1386"
                     /culture_collection="FDA:FDAARGOS 1386"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            <1..180
                     /locus_tag="I6L16_25165"
     CDS             <1..180
                     /locus_tag="I6L16_25165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="QXB17483.1"
                     /translation="ESAGAALERADRTLEPVIQREQEIRDERLMQEREHELSLERERQP
                     EIQERTLDGPSLGW"
                     /name="nuclease"
     gene            554..1606
                     /locus_tag="I6L16_25170"
     CDS             554..1606
                     /locus_tag="I6L16_25170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXB17484.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIESSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            1608..2474
                     /locus_tag="I6L16_25175"
     CDS             1608..2474
                     /locus_tag="I6L16_25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="QXB17485.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(3674..3865)
                     /locus_tag="I6L16_25180"
     CDS             complement(3674..3865)
                     /locus_tag="I6L16_25180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QXB17486.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3929..4252
                     /locus_tag="I6L16_25185"
     CDS             3929..4252
                     /locus_tag="I6L16_25185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QXB17487.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4242..>5544
                     /locus_tag="I6L16_25190"
     CDS             4242..>5544
                     /locus_tag="I6L16_25190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="QXB17488.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELID
                     ATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLE
                     LNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNL
                     PETKQALAEGVTRGIDALYHAGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGRN
                     IRLKGAFYEQSFTDGRGVREKAERESRIYRENAERRVQEARRICKQGTDLKRIFNQERY
                     QRPYNSPERTAGEVSENDRRYDGRALENRTENERETLAVADYRLSTDVCHDRESAVVPG
                     HEDKREYEYSPRAERESGEAEREDMGRALSRGQQRTFSGVAEGDESRNELDEGQRQTER
                     SETGAGVTEHDGAGKTFAERIRATATGLYAAAERM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          3502..3589
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          2858..3045
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          4243..5543
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 gagtcagcag gtgcagcact tgagcgagcg gacagaacgc ttgagccagt tatacagcga
       61 gaacaggaga taagggatga acggctgatg caggaacgcg aacatgagtt atctctggag
      121 cgggagcgtc agccggaaat ccaggagcgg acactggatg gcccttcgct gggatggtga
      181 aaccatgaaa aatgacgtgt tcagtggatt aaggcgggct aatgtggcct gtagcatctg
      241 gttgcatagg tattcatgcg gttaaaattt atcaggtgcg atcgcggcag tttttcgggt
      301 ggtttgttgc catttttacc tgtctgctgc cgtgatcgca ccgaacgcgt tttggcggtg
      361 cgtacaatgc gacgttatgg taaatggagc ggattttcag gaaggagtga tactaacaac
      421 tcgaacactg tgcaaataat gattttttta aaatatcggt aacgataata aattatttcg
      481 ttttacgccg gtaataaata taatgtagtt tcagtatata aaaaaatata tgaggtgtct
      541 tgtgggagct ttcatgaatc ttcaacaacg tataaataaa ctaccacaat tatcatccag
      601 tttttcattt ggaaaagata ttgataatat tcattcattt atctttaacg aaacttctaa
      661 agataaaatt gaagatttac taagaaaatg ggtgtcaggc aatcagccct gtgtatttgg
      721 taaattggca agcaagaaaa ttaaagggct tgatttccat ttatctattg taaatagccc
      781 ccaactatat aatgatgatg gtcatttgtt tgattttttg cgtaatgaaa gagttcgttt
      841 taaagaacgc gcaagaagag gagaggtcag tgcgcatctc atttatttta ttcatccaca
      901 acttgccttt gctcgtccat cagaagagtt agtggacata caaaaatata tttgttcgct
      961 gcatatgcca gagtgctatc caataaaaga ggatgttatt tatactgaaa gcgtaccatt
     1021 tcaggataaa gatgggctaa aaatatataa ggctggtgtt aatgtttttt actcttctgc
     1081 tcatagaaca agaaatcatg atagaagaat accaggggga atacttattt ctgtaaatgc
     1141 cccggggcat tttatgaggc tggcgataga aaaaggtttc tataaagatc aagaacaagc
     1201 gttagcggat attcgtaaca tgactattca gtctgttggt aagggtgggt attcacatcc
     1261 ggaagggatt tctacaacat ggcattcaga atctaatttg gatcgttttg gatgcccagt
     1321 gcacaccggt aatagttctt attacagcgg tttttatcat actgatgtat taattccagg
     1381 agaattaact aaggatgaaa gattgctcca tgaaatagaa agtagtgatc caatgatctt
     1441 caactggaat gtattgtttt atgtttcatt agaagagttt cctatagatg atccttatta
     1501 tggagagttt attggtattc ctgtagatga tgcatctatg ttttttaact cctttcaacc
     1561 aaggaaattt gaaaacaaac ccctttatga gaaagaggat gattaatatg attaaaggaa
     1621 tgctttttgc ttttttagca gcgttctcat ggggggcagc tatcgtaatg agcaaaaaag
     1681 gagttgaaga actagatgcc ggaggggtgt ttttctggca gatttgtagt gcagtaatac
     1741 tatcttggat agttcttttt ataaaaagaa aaaaactccc attaaactca aaagcgatac
     1801 ttgcttatgc gacaggcgtt tttgaaccat tcttagcgta tacttttaca ctctacggac
     1861 tttattttat ttcagcagga agcgcatcaa ttattttttc acttgaatca gtgtttatat
     1921 tggtgttgtc tgttcttatt tgctcagtta aaattaattc cccgcaatat tttatattgt
     1981 tccttatagg tgcaattgtt ggcagtattt tggtagcctt gccagacatc agtaatggat
     2041 cgaataatat tgttggatat tgtcttgttg ttgccggtgt gttatcggca gcattttatg
     2101 ttgttatatc gtctcgcctt attaaatcgt ttgagcctat cacattattg agcggacagt
     2161 tgacattctc ccttatactg tcatcgctct ttatttttat atctaaatca tcaatttatc
     2221 ttccgttgga atccgtgatt attgtttttt catctggaat attacaatat tttttagctt
     2281 tttgttttta cttgaactcc ttgcgttggc tcccagttca tttggcaggg ataatgttgt
     2341 actttattcc gctagttgca ttatttttgt catggttgtt tttggatgaa caattcacat
     2401 tcttacaggg ggtaggattt attgtaactg tgctaagtgt atattttctt aacagaaaat
     2461 actcagagga gtaacattct gttcaggatt gccccccggc ctccatcgat accgtgccaa
     2521 atgcacgtaa aaaacgggag cgtttctggt gtcggaatgt aaccaagggg gtcagaggcg
     2581 cgctaaaacg ctctcagggg agtcaaatag agacgtttgt aacaaaaaga tcaaaggatc
     2641 ctcttgagat cctttttttc tgcgcgtaat ctgctgcctg taaacgaaaa aaccgccttg
     2701 cagggcggtt tgatcgaagg ttaagtcagc ttgcagactg cttaaccagg taactgtctt
     2761 atgcgagacg agtcaccaaa attgttcttc tagtgtagcc gcactccggc agccacttca
     2821 agtactctct aaatatctcg cacatgactt ttaccaatgg ctgctgccag tggcgctttg
     2881 tggtgtcttt tccgggttgg acccaagaga acagttaccg gagaaggcgc agcagtcggg
     2941 ctgaacgggg ggttcctgca tacagcccag cccggagcga actgcctaag tcgccccgag
     3001 ataccagaag acgtgagcta tgagaaagcg ccacgtgtcc cgtaagggag aaaggcggaa
     3061 aaggtatccg gtaatcgata tggcgggaac gtgggggcgc aggagggagc gattcaccgg
     3121 aaacggtgat gatctttaag ccacgtcggg tttcgccacc gctggcggat ttcatggttg
     3181 agccgcaagg ctcccacaga tgcaccgaaa aagtgtctgt tttgtgaagt ccgtcagggg
     3241 ggcggagcct atggaaaaag cgcctccggc gcggcctttc ccggggtttc gttttttgcg
     3301 cacatgttct ttcctggttt atccccgggt tctgtggata acagggttag cgtttttgag
     3361 aaaggagagt ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa
     3421 gcggaagagc gcctgaactg gcattttctc cttacgcatc tgtgcggcat ttcacaccgg
     3481 acatggcgca cttttcatac aatccgcact gatgccgaat ggttaagcca gtatacactc
     3541 cgctatcgct acgtgactgg gtcagggctg cgccccgaca cccgctaaaa cctgctgacg
     3601 cgccctgacg ggcttgtcag ctcccggcat ccgctcacag acaagctgtg accgtctccg
     3661 ggagctgcat gtatcagaga ttttcaccgt catccccgaa acgtgcgagg cagctgcggt
     3721 aaagctcatc ggcgtggtcg tgaagcgatt cacaaatatc ggcctgttca tctgcgtcca
     3781 gttcgttgag cttctccagc agcgttaatg tctggcttct gataaagcgg gccatgttaa
     3841 gggcggtttt ttcctgttta gtcactgatg cctccgtgta agggggattt ctgttcatgg
     3901 ggtaatgata ccgatgaaac gcgagaggat gctcacaata cgggttactg atgatgaaca
     3961 tgcccggtta cttgaacgct gtgagggtaa acaactggcg gtatggatgc ggcgggtctg
     4021 cctgggggag ccggttgccc gttccggaaa actgccgaca ctggcaccgc cgttactgcg
     4081 tcagctggcc gccatcggaa ataacctgaa tcagacagcc cgtaaggtga acagcgggca
     4141 gtggtcttcc ggtgaccggg ttcaggtggt ggccgcactg atggccatcg gggatgagct
     4201 gcgccggctg cgtctggccg tcagggaaca gggggcgcgg gatgatagtt aaatttcatg
     4261 ccaggggaaa aggtggtggc agtggtccgg ttgattacct gctggggagg gagcgtaacc
     4321 gcgaaggcgc aacggtgctt cagggtaatc cggaagaagt ccgggaactc atcgatgcca
     4381 cgccatttgc gaagaaatac acgtccggtg ttctgtcgtt cgcggagaag gagctgccgc
     4441 cgggaggacg tgaaaaagtg atggcgagct ttgagcgtgt actgatgccc ggtctcgaaa
     4501 aaaatcagta cagcatcctg tgggtggagc accaggacaa gggacggctt gagctgaatt
     4561 ttgtcattcc gaacatggag ctacagaccg gaaaacgcct ccagccgtac tacgaccgcg
     4621 cagacaggcc tagaattgat gcctggcaga cgctggtgaa tcaccattac gggctgcatg
     4681 acccgaacgc cccggagaac cgccggacgc tgacccttcc tgataacctg ccggaaacga
     4741 aacaggcgct tgctgagggc gtcacacgcg ggatagacgc gctttaccat gccggagaga
     4801 taaaaggccg tcaggatgtg attcaggcgc tcacagaggc ggggcttgaa gtggtcaggg
     4861 tgacgcgaag cagtatcagc attgcagacc cgaacggcgg gaggaatatc agactgaaag
     4921 gagcgtttta tgagcaatct tttacagacg ggcgcggagt tcgagaaaaa gctgaaagag
     4981 agagccgaat ctacagagaa aatgctgaac gacgagttca ggaagctcga agaatctgta
     5041 aacagggaac tgacctcaaa cgaatcttta atcaggaacg ctatcaacga ccatacaaca
     5101 gccctgaaag aactgctgga gaggtatcag aaaacgaccg tcgatacgat ggacgcgcac
     5161 tggaaaaccg tactgaaaat gagcgtgaaa cgctggctgt ggctgattat cgtctcagta
     5221 ctgatgtttg ccacgaccgg gagtctgctg tggtaccagg gcatgaagat aaacgcgaat
     5281 atgaatattc tccgcgagca gaaagagagt ctggagaagc tgaacgcgaa gacatggggc
     5341 gtgcgttatc acgaggacag caacggacgt tttctggtgt tgccgaaggg gatgaaagcc
     5401 gaaacgaact ggacgaagga caacggcaaa ctgaacgcag tgagactggt gcaggagtga
     5461 cagagcatga cggagctgga aagacatttg ctgaacgcat tagagcaact gcaacaggac
     5521 tatatgcagc ggctgaacga atgg
//
