LOCUS       CP077296                2443 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS 1386 plasmid unnamed2, complete
            sequence.
ACCESSION   CP077296
VERSION     CP077296.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357528
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1865.33x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:25:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,049
            CDSs (total)                      :: 4,933
            Genes (coding)                    :: 4,776
            CDSs (with protein)               :: 4,776
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 68 of 157
            Pseudo Genes (incomplete)         :: 83 of 157
            Pseudo Genes (internal stop)      :: 36 of 157
            Pseudo Genes (multiple problems)  :: 28 of 157
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1386"
                     /culture_collection="FDA:FDAARGOS 1386"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            join(2300..2443,1..393)
                     /locus_tag="I6L16_25160"
     CDS             join(2300..2443,1..393)
                     /locus_tag="I6L16_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="QXB17482.1"
                     /translation="MDEVAKWRPFTDQDGNIWDLSFLDAHEVSYTHCSPGKPDIVYKFT
                     VSYSFHCFCKDYPEQTEEEKLSLMYDAGKDSRPFCKVRYALARDKLRAIIEGLGNAKVI
                     HAGYGSYAFVEITVDEGESEFYFVPFKVYRERKKLRLHVTSAYPVKERPSGKPVSFFKI
                     AHNLLRGKPLPKPHM"
                     /name="stationary phase growth adaptation protein"
     Typing          complement(855..1277)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.529"
                     /coverage="80.0"
                     /evalue="4.19e-164"
ORIGIN
        1 tcatttcatt gtttttgcaa ggattatcca gagcagactg aggaagaaaa attatcgttg
       61 atgtatgatg caggcaaaga ctcgaggcca ttttgtaagg tccgatatgc ccttgctcga
      121 gacaagctga gagccatcat tgaaggattg ggtaacgcga aggttatcca tgctggatat
      181 ggtagttatg cttttgtaga gattactgta gatgaaggtg aaagtgagtt ctactttgtg
      241 ccttttaagg tatatcgtga gcgtaaaaag ctgcgcttgc atgtaacaag tgcttatcct
      301 gtcaaggaga gaccgtcagg taagccagtt agctttttca agatagctca caatctcctg
      361 cgcgggaagc cattacctaa acctcatatg taacaaagcc cgcttagttg cgggcttcgc
      421 cagaaatttc ttctggtatt cagaacacct ttaccgggct tggcctcgac caacattgtt
      481 attggtagtg ggatgtctgg tgttaccagt ctgctgcatc cttaaagcaa ttgtaggtag
      541 tgtgatgtag atttgcaacc aacattttgg ttgcaaccta tatactaatt tttctcgata
      601 aactcggaat ccattgctgt atctggcttt cttgaagctt ctgactatag atcccctata
      661 ccctaacagt tattctgctg tggatatcag aaatgaacac ttgagatcat ctgcggctcg
      721 tcgtaatctc ttgcttagaa acgaaaaaac cacctgggga ggtggttttt tcgatggttc
      781 agtaagttgg ggaacttctg aaccgtggta acagggtgta caagaccgct gccaccaaat
      841 tcgtccttct agtttagccg tagtttggtt ctaacttcaa gaactctgca tcagtaatct
      901 cttgtacacc ccttaccaat ggccgcagcc agtggcgata agtcgtgtct ttccgggttg
      961 gactcaagat gatagttacc ggacaaggcg cagcggtcgg actgaacggg gggttcgtgc
     1021 atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga
     1081 caaacgcggc cataacagcg gagtgacacc ggtaaaccga atggcaggaa caggagagcg
     1141 cacgagggag ccgccagggg gaaacgcctg gtatctttaa gtcctgtcgg gtttcgccac
     1201 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1261 gccagcggcg cggccttttt ttgccttttt gctcacatgt tctttccggc gttatcctct
     1321 gattctgtgg ataaccacat tatcgttttt gagtgagcag acaccgctcg ccgcagccga
     1381 acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atcttcgtac ttatctttgg
     1441 caccgtacac gccatagctg catttttaat gcgatgcagg atagggctaa cgccgtaaag
     1501 tgacgtcacc tgatacttct ggattacaac agttaaagca gatgacagga tatgttttgt
     1561 gtccaccgcc agtttgtcag cgctacaaaa cagcgggaca agatgtgttt ttggagtacc
     1621 gcccatactc caaaacgcta tgaccatgct ctgcccgata cccttagcaa tcagtgtttg
     1681 caatctgtcc cgaatctgtt tgctcagtgt ttatagcctg ttttctcact gatttactct
     1741 gcatgtccaa ggcacgttag cgatgtatac atcgccgcgc cgcaagggtg ccccttccga
     1801 atcccgttca tcactgcgtg ctgattaatc cacgttaaat ccgaccccct tagcaataaa
     1861 tccagccatg cctgacggcc tggccaacgc ctcacggccg cacagtaaac tgcacacccg
     1921 tgaggcgtta ttcgtgaggt tcagtggtgc ttttgggcgt ccagccggat tttagcggct
     1981 aaaatcccgg ccatgcatgt gtgtgcatag ccatgcagac gcgccagaat catcaggcgc
     2041 gttttttcac gatataggca cagggaagtg tcggaaacag gcaggaagac ccagaaacgc
     2101 cctgagggcc tctcaggcgg tgcacgcaac ggatgttatg gtaaataaca cttaatcatt
     2161 accaatgtat ccatgaaagg ttgtggcgaa gtcttctgta aaaggcctag ctcttatttt
     2221 gccacgctcc ttccgcttgt ttcttctctg gtagaatgtg cggattctgc catccggata
     2281 ttgaggtttt ttcgtgaaca ttgatgaagt tgctaagtgg cgtcctttta cggatcagga
     2341 tggcaatata tgggatctgt catttcttga tgcacatgag gtttcttata ctcattgttc
     2401 tcctggtaaa cctgatattg tgtataagtt cactgtttct tac
//
