LOCUS       CP077289                5655 bp    DNA     linear   BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1388 plasmid unnamed8.
ACCESSION   CP077289
VERSION     CP077289.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357530
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 769.82x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:23:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,178
            CDSs (total)                      :: 5,048
            Genes (coding)                    :: 4,708
            CDSs (with protein)               :: 4,708
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 340
            CDSs (without protein)            :: 340
            Pseudo Genes (ambiguous residues) :: 0 of 340
            Pseudo Genes (frameshifted)       :: 145 of 340
            Pseudo Genes (incomplete)         :: 190 of 340
            Pseudo Genes (internal stop)      :: 35 of 340
            Pseudo Genes (multiple problems)  :: 27 of 340
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1388"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1388"
                     /db_xref="taxon:562"
                     /plasmid="unnamed8"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            <1..177
                     /locus_tag="I6L18_25860"
     CDS             <1..177
                     /locus_tag="I6L18_25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QXA64920.1"
                     /translation="KTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYR
                     SCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1380..2246)
                     /locus_tag="I6L18_25865"
     CDS             complement(1380..2246)
                     /locus_tag="I6L18_25865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="QXA64915.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(2248..3300)
                     /locus_tag="I6L18_25870"
     CDS             complement(2248..3300)
                     /locus_tag="I6L18_25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA64916.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFICIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(3780..4013)
                     /locus_tag="I6L18_25875"
     CDS             complement(3780..4013)
                     /locus_tag="I6L18_25875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QXA64917.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4020..4550)
                     /locus_tag="I6L18_25880"
     CDS             complement(4020..4550)
                     /locus_tag="I6L18_25880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QXA64918.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(4528..5286)
                     /locus_tag="I6L18_25885"
                     /pseudo=""
     CDS             complement(4528..5286)
                     /locus_tag="I6L18_25885"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5228..5551)
                     /locus_tag="I6L18_25890"
     CDS             complement(5228..5551)
                     /locus_tag="I6L18_25890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QXA64919.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(272..353)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(810..997)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(3677..5238)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct gctggagaag
       61 ctcaacgaac tggacgcgga tgaacaggca gatatctgtg aatcgcttca cgaccacgcc
      121 gatgagcttt accgcagttg cctcgcacgc ttcggggatg acggtgaaaa ccgctgacac
      181 atgcagctcc cggagacggt cacagcttgt ctgtgagcgg atgccgggag cagacaagcc
      241 cgccagggcg cgtcaggccg ttgacctttg gggtgtcggg gcgaagccct gacccagtca
      301 cgtagcgata gcggagtgta tactggctta accattcggc atcagtgcgg attgtatgaa
      361 aagtgcgcca tgtccggtgt gaaatgccgc acagatgcgt aaggagaaaa tgccagttca
      421 ggcgctcttc cgcttcctcg ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag
      481 cggactctcc tttctcaaaa acgctaaccc tgttatccac agaacccggg gataaaccag
      541 gaaagaacat gtgcgcaaaa aacgaaaccc cgggaaaggc cgcgccggag gcgctttttc
      601 cataggctcc gcccccctga cggacttcac aaaacagaca ctttttcggt gcatctgtgg
      661 gagccttgcg gctcaaccat gaaatccgcc agcggtggcg aaacccgacg tggcttaaag
      721 atcatcaccg tttccggtga atcgctccct cctgcgcccc cacgttcccg ccatatcgat
      781 taccggatac cttttccgcc tttctccctt acgggacacg tggcgctttc tcatagctca
      841 cgtcttctgg tatctcgggg cgacttaggc agttcgctcc gggctgggct gtatgcagga
      901 accccccgtt cagcccgact gctgcgcctt ctccggtaac tgttctcttg ggtccaaccc
      961 ggaaaagaca ccacaaagcg ccactggcag cagccattgg taaaagtcat gtgcgagata
     1021 tttagagagt acttgaagtg gctgccggag tgcggctaca ctagaagaac aattttggtg
     1081 actcgtctcg cataagacag ttacctggtt aagcagtctg caagctgact taaccttcga
     1141 tcaaaccgcc ctgcaaggcg gttttttcgt ttacaggcag cagattacgc gcagaaaaaa
     1201 aggatctcaa gaggatcctt tgatcttttt gttacaaacg tctctatttg actcccctga
     1261 gagcgtttta gcgcgcctct gacccccttg gttacattcc gacaccagaa acgctcccgt
     1321 tttttacgtg catttggcac ggtatcgatg gaggccgggg ggcaatcctg aacagaatgt
     1381 tactcctctg agtattttct gttaagaaaa tatacactta gcacagttac aataaatcct
     1441 accccctgta agaatgtgaa ttgttcatcc aaaaacaacc atgacaaaaa taatgcaact
     1501 agcggaataa agtacaacat tatccctgcc aaatgaactg ggagccaacg caaggagttc
     1561 aagtaaaaac aaaaagctaa aaaatattgt aatattccag atgaaaaaac aataatcacg
     1621 gattccaacg gaagataaat tgatgattta gatataaaaa taaagagcga tgacagtata
     1681 agggagaatg tcaactgtcc gctcaataat gtgataggct caaacgattt aataaggcga
     1741 gacgatataa caacataaaa tgctgccgat aacacaccgg caacaacaag acaatatcca
     1801 acaatattat tcgatccatt actgatgtct ggcaaggcta ccaaaatact gccaacaatt
     1861 gcacctataa ggaacaatat aaaatattgc ggggaattaa ttttaactga gcaaataaga
     1921 acagacaaca ccaatataaa cactgattca agtgaaaaaa taattgatgc gcttcctgct
     1981 gaaataaaat aaagtccgta gagtgtaaaa gtatacgcta agaatggttc aaaaacgcct
     2041 gtcgcataag caagtatcgc ttttgagttt aatgggagtt tttttctttt tataaaaaga
     2101 actatccaag atagtattac tgcactacaa atctgccaga aaaacacccc tccggcatct
     2161 agttcttcaa ctcctttttt gctcattacg atagctgccc cccatgagaa cgctgctaaa
     2221 aaagcaaaaa gcattccttt aatcatatta atcatcctct ttctcataaa ggggtttgtt
     2281 ttcaaatttc cttggttgaa aggagttaaa aaacatagat gcatcatcta caggaataca
     2341 aataaactct ccataataag gatcatctat aggaaactct tctaatgaaa cataaaacaa
     2401 tacattccag ttgaagatca ttggatcact attttctatt tcatggagca atctttcatc
     2461 cttagttaat tctcctggaa ttaatacatc agtatgataa aaaccgctgt aataagaact
     2521 attaccggtg tgcactgggc atccaaaacg atccaaatta gattctgaat gccatgttgt
     2581 agaaatccct tccggatgtg aatacccacc cttaccaaca gactgaatag tcatgttacg
     2641 aatatccgct aacgcttgtt cttgatcttt atagaaacct ttttctatcg ccagcctcat
     2701 aaaatgcccc ggggcattta cagaaataag tattccccct ggtattcttc tatcatgatt
     2761 tcttgttcta tgagcagaag agtaaaaaac attaacacca gccttatata tttttagccc
     2821 atctttatcc tgaaatggta cgctttcagt ataaataaca tcctctttta ttggatagca
     2881 ctctggcata tgcagcgaac aaatatattt ttgtatgtcc actaactctt ctgatggacg
     2941 agcaaaggca agttgtggat gaataaaata aatgagatgc gcactgacct ctcctcttct
     3001 tgcgcgttct ttaaaacgaa ctctttcatt acgcaaaaaa tcaaacaaat gaccatcatc
     3061 attatatagt tgggggctat ttacaataga taaatggaaa tcaagccctt taattttctt
     3121 gcttgccaat ttaccaaata cacagggctg attgcctgac acccattttc ttagtaaatc
     3181 ttcaatttta tctttagaag tttcgttaaa gataaatgaa tgaatattat caatatcttt
     3241 tccaaatgaa aaactggatg ataattgtgg tagtttattt atacgttgtt gaagattcat
     3301 gaaagctccc acaagacacc tcatatattt tttatatact gaaactacat tatatttatt
     3361 accggcgtaa aacgaaataa tttattatcg ttaccgatat tttaaaaaaa tcattatttg
     3421 cacagtgttc gagttgttag tatcactcct tcctgaaaat ccgctccatt taccataacg
     3481 tcgcattgta cgcaccgcca aaacgcgttc ggtgcgatca cggcagcaga caggtaaaaa
     3541 tggcaacaaa ccacccgaaa aactgccgcg atcgcacctg ataaatttta accgtatgaa
     3601 tacctatgca accagatgct acaggccaca ttagcccgcc ttaatccact gaacacgtca
     3661 tttttcatgg tttcaccatc ccagcgaagg gccatccagt gtccgctcct ggatttccgg
     3721 ctgacgctcc cgctccagag ataactcatg ttcgcgttcc tgcatcagcc gttcatccct
     3781 tatctcctgt tctcgctgta taactggctc aagcgttctg tccgctcgct caagtgctgc
     3841 acctgctgac tcaactgcat gactcgcccg ttcagcatcg cgttgtcccg ttgcgtaagc
     3901 gaaaacattt tctgcaattc cacgaaggcg ttctcccatt cgttcagccg ctgcatatag
     3961 tcctgttgca gttgctctaa tgcgttcagc aaatgtcttt ccagctccgt catgctctgt
     4021 cactcctgca ccagtctcac tgcgttcagt ttgccgttgt ccttcgtcca gttcgtttcg
     4081 gctttcatcc ccttcggcaa caccagaaaa cgtccgttgc tgtcctcgtg ataacgcacg
     4141 ccccatgtct tcgcgttcag cttctccaga ctctctttct gctcgcggag aatattcata
     4201 ttcgcgttta tcttcatgcc ctggtaccac agcagactcc cggtcgtggc aaacatcagt
     4261 actgagacga taatcagcca cagccagcgt ttcacgctca ttttcagtac ggttttccag
     4321 tgcgcgtcca tcgtatcgac ggtcgttttc tgatacctct ccagcagttc tttcagggct
     4381 gttgtatggt cgttgatagc gttcctgatt aaagattcgt ttgaggtcag ttccctgttt
     4441 acagattctt cgagcttcct gaactcgtcg ttcagcattt tctctgtaga ttcggctctc
     4501 tctttcagct ttttctcgaa ctccgcgccc gtctgtaaaa gattgctcat aaaacgctcc
     4561 tttcagtctg atattcctcc cgccgttcgg gtctgcaatg ctgatactgc ttcgcgtcac
     4621 cctgaccact tcaagccccg cctctgtgag cgcctgaatc acatcctgac ggccttttat
     4681 ctctccggca tggtaaagcg cgtctatccc gcgtgtgacg ccctcagcaa gcgcctgttt
     4741 cgtttccggc aggttatcag gaagggtcag cgtccggcgg ttctccgggg cgttggggtc
     4801 atgcagaccg taatggtgat tcaccagcgt ctgccaggca tcaattctcg gcctgtctgc
     4861 ccggtcgtag tacggctgga ggcgttttcc ggtctgtagc tccatgttcg gaatgacaaa
     4921 attcagctca agccgtccct tgtcctggtg ctccacccac aggatgctgt actgattttt
     4981 ctcgagaccg ggcatcagta cacgctcaaa gctcgccatc actttttcac gccctcccgg
     5041 cggcagctcc ttctccgcga acgacagaac accggacgtg tatttcttcg caaatggcgt
     5101 ggcatcgatg agttcccgga cttcttccgg attaccctga agcaccgttg ccccttcccg
     5161 gttacgctcc cttcccagca ggtaatcaac cggaccactg ccaccacctt ttcccctggc
     5221 atgaaactta actatcatcc cgcgccccct gttccctgac ggccagacgc aaccggcgca
     5281 gctcatcccc gatggccatc agtgcggcca ccacctgaac ccggtcgccg gaagaccact
     5341 gtccgctgtt caccttacgg gctgcctggt tcaggttatt cccgatggct gccagctgac
     5401 gcagtaacgg cggtgccagt gtcggcagcc tcccggaacg ggcaaccggc tcacccaggc
     5461 agacccgccg catccatacc gccagttgtt taccctcaca gcgttccagt aaccgggcat
     5521 gttcatcatc agtaacccgt atcgtgagca tcctctcgcg tttcatcggt atcattaccc
     5581 ccatgaacag aaatccccct tacacggagg catcagtgac taaacaggaa aaaaccgccc
     5641 ttaacatggc ccgct
//
