LOCUS       CP077288                6647 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1388 plasmid unnamed7, complete
            sequence.
ACCESSION   CP077288
VERSION     CP077288.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357530
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 769.82x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:23:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,178
            CDSs (total)                      :: 5,048
            Genes (coding)                    :: 4,708
            CDSs (with protein)               :: 4,708
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 340
            CDSs (without protein)            :: 340
            Pseudo Genes (ambiguous residues) :: 0 of 340
            Pseudo Genes (frameshifted)       :: 145 of 340
            Pseudo Genes (incomplete)         :: 190 of 340
            Pseudo Genes (internal stop)      :: 35 of 340
            Pseudo Genes (multiple problems)  :: 27 of 340
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1388"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1388"
                     /db_xref="taxon:562"
                     /plasmid="unnamed7"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            193..630
                     /locus_tag="I6L18_25810"
     CDS             193..630
                     /locus_tag="I6L18_25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QXA64909.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSKGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            711..1130
                     /locus_tag="I6L18_25815"
     CDS             711..1130
                     /locus_tag="I6L18_25815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QXA64910.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(1448..1681)
                     /locus_tag="I6L18_25820"
     CDS             complement(1448..1681)
                     /locus_tag="I6L18_25820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QXA64911.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1688..2206)
                     /locus_tag="I6L18_25825"
     CDS             complement(1688..2206)
                     /locus_tag="I6L18_25825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeB"
                     /protein_id="QXA64912.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="mobilization protein MbeB"
     gene            complement(2184..2942)
                     /locus_tag="I6L18_25830"
                     /pseudo=""
     CDS             complement(2184..2942)
                     /locus_tag="I6L18_25830"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2884..3207)
                     /locus_tag="I6L18_25835"
     CDS             complement(2884..3207)
                     /locus_tag="I6L18_25835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QXA64913.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3271..3462
                     /locus_tag="I6L18_25840"
     CDS             3271..3462
                     /locus_tag="I6L18_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QXA64914.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4489..4626)
                     /locus_tag="I6L18_25845"
     CDS             complement(4489..4626)
                     /locus_tag="I6L18_25845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QXA64906.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            4674..5015
                     /locus_tag="I6L18_25850"
     CDS             4674..5015
                     /locus_tag="I6L18_25850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="QXA64907.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(5012..6580)
                     /gene="cea"
                     /locus_tag="I6L18_25855"
     CDS             complement(5012..6580)
                     /gene="cea"
                     /locus_tag="I6L18_25855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="QXA64908.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     Typing          complement(3548..3635)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          3646..4204
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1345..2894)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttatatacag cataaaagct acgccgctgc attttccctg tcaagactgt ggacttccat
       61 ttttgtgaaa acgatcaaaa aaacagtctt tcacaccacg cgctattctc gcccgatgcc
      121 acaaaaacca gcacaaacat taccgttctc agacctcatt atgttttact gaaactatga
      181 gatgagacat ctatgggaca ctgtcacttt atggcatggc acacactccg ggacgcacta
      241 aaaatgacag gcagatcgcg ttcacagttt taccgtgata tgcgcgcagg ccttgtcagt
      301 taccgtaccg gcagggacgg acgacgggag tttgaaacca gtgaactgat ccgggcatac
      361 ggcgaattaa agcagaatga gacaccagaa aggcacagta agggacatgc agaaaatcca
      421 catgatcagc agacagaacg cattctccgg gagctgaatg agctgaaaca atgcctgacg
      481 ctgatgcttg aggataaaca ggcacaggat atggatcgca gacgccagga agcagaacgg
      541 gaacagctac aaaatgagat agcccagctc aggcaggcac tggaactgga aaagaaacgg
      601 ggattctggt ccaggttgtt cggtcgctga acgctgtcag agactgatga taaaatagtc
      661 ttcggataat aactcaccga gaataaatac tttaaggtag ggagacactc atgagacgta
      721 ccggaaacaa actttgtctt atcgccatga taacagcaac agtagctctc acagcctgta
      781 ccccaaaggg cagcgtggaa caacataccc ggcattacgt atatgcttct gatgacggtt
      841 ttgatcccaa cttttccacc caaaaagccg acacaacacg aatgatggtg cctttttttc
      901 ggcagttctg ggatatggga gctaaagata aagcgacagg aaaatcacgg agtgatgtgc
      961 aacaacgcat tcagcagttt cacagccagg aatttttaaa ctcactccgg ggcacaactc
     1021 aatttgcggg tactgattac cgcagcaaag accttacccc gaaaaaatcc aggctgctgg
     1081 ctgacacgat ttctgcggtt tatctcgatg gctacgaggg cagacagtaa gtggatttac
     1141 cataatccct taattgtacg caccgctaaa acgcgttcag cgcgatcacg gcagcagaca
     1201 ggtaaaaatg gcaacaaacc acccgaaaaa ctgccgcgat cgcgcctgat aaattttaac
     1261 cgtatgaata cctatgcaac cagagggtac aggccacatt acccccactt aatccactga
     1321 agctgccatt tttcatggtt tcaccatccc agcgaagggc catccagcgt gcgttcctgt
     1381 atttccggct gacgctcccg ttccagggat aacacatgtt cgcgctcctg tatcagccgt
     1441 tcctctctta tctccagttc tcgctgtata actggctcaa gcgttctgtc tgctcgctca
     1501 agtgctgcac ctgctgactc aactgcatga cccgctcgtt cagcatcgcg ttgtcccgtt
     1561 gcgtaagcga aaacatcttc tgcaattcca cgaaggcgct ctcccattcg ctcagccgct
     1621 gcatatagtc ctgttgcagc tgctctaagg cgttcagcaa atgtgtttcc agctctgtca
     1681 ctctgtgtca ctccttcaga tgtacccact ctttcccctg aaagggaatc acctccgctg
     1741 atttcccgta cggaaggaca aggaatttcc tgttcccgtc ctgcacaaac tccacgcccc
     1801 atgtcttcgc gttcagtttc tgcaatgtct cttcctgctt cctgatttct tccaggttcg
     1861 cctgtatcct ccctccaaga taccagagcg tcccgccact cgcggtaaac aggagaaaga
     1921 ctatccccag taacatcatg cccgtattcc ctgccagctt taacacgtcc ctcctgtgct
     1981 gcatcatcgc ctctttcacc ccttcccggt gtttttccag cgattcctct gtcgaggctg
     2041 tgaacagggc tatagcgtct ctgattttcg tctcgtttga tgtcacagcc tcgcttacag
     2101 attcgccgag cctcctgaac tcgttgttca gcattttctc tgtagattcg gctctctctt
     2161 tcagcttttt ctcgaactcc gcgcccgtct gcaaaagatt gctcataaaa tgctcctttc
     2221 agcctgatat tcttcccgcc gttcggatct gcaatgctga tactgcttcg cgtcaccctg
     2281 accacttcca gccccgcctc agtgagcgcc tgaatcacat cctgacggcc ttttatctct
     2341 ccggcatggt aaagtgcatc tatacctcgc gtgacgccct cagcaagcgc ctgtttcgtt
     2401 tcaggcaggt tatcagggag tgtcagcgtc ctgcggttct ccggggcgtt cgggtcatgc
     2461 agcccgtaat ggtgattcac cagcgtctgc caggcatcaa ttctaggcct gtctgcgcgg
     2521 tcgtagtacg gctggaggcg ttttccggtc tgtagctcca tgttcggaat gacaaaattc
     2581 agctcaagcc gtcccttgtc ctggtgctcc acccacagga tgctgtactg atttttttcg
     2641 agaccgggca tcagtacacg ctcaaagctc gccatcactt tttcacgtcc tcccggcggc
     2701 agctccttct ccgcgaacga cagaacaccg gacgtgtatt tcttcgcaaa tggcgtggca
     2761 tcgatgagtt cccggacttc ttccggatta ccctgaagca ccgttgcgcc ttcgcggtta
     2821 cgctccctcc ccagcaggta atcaaccgga ccactgccac caccttttcc cctggcatga
     2881 aatttaacta tcatcccgcg ccccctgttc cctgacagcc agacgcagcc ggcgcagctc
     2941 atccccgatg gccatcagtg cggccaccac ctgaacccgg tcaccggaag accactgccc
     3001 gctgttcacc ttacgggctg tctgattcag gttatttccg atggcggcca gctgacgcag
     3061 taacggcggt gccagtgtcg gcagttttcc ggaacgggca accggctccc ccaggcagac
     3121 ccgccgcatc cataccgcca gttgtttacc ctcacagcgt tcaagtaacc gggcatgttc
     3181 atcatcagta acccgtattg tgagcatcct ctcgcgtttc atcggtatca ttaccccatg
     3241 aacagaaatc ccccttacac ggaggcatca gtgactaaac aggaaaaaac cgcccttaac
     3301 atggcccgct ttatcagaag ccagacatta acgctgctgg agaagctcaa cgaactggac
     3361 gcagatgaac aggccgatat ttgtgaatcg cttcacgacc acgccgatga gctttaccgc
     3421 agctgcctcg cacgtttcgg ggatgacggt gaaaacctct gacacatgca gctcccggag
     3481 acggtcacag cttgtctgtg agcggatgcc gggagctgac aagcccgtca gggcgcgtca
     3541 gcaggtttta gcgggtgtcg gggcgcagcc ctgacccagt cacgtagcga tagcggagtg
     3601 tatactggct taaccatgcg gcatcagtgc ggattgtatg aaaagtacgc catgccgggt
     3661 gtgaaatgcc gcacagatgc gtaaggagaa aatgcacgtc caggcgcttt tccgcttcct
     3721 cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggtactg actcacacaa
     3781 aaacggtaac acagttatcc acagaatcag gggataaggc cggaaagaac atgtgagcaa
     3841 aagaccagga acaggaagaa ggccacgtag caggcgtttt tccataggct ccgcccccct
     3901 gacgagcatc acaaaaatag acgctcaagt cagaggtggc gaaacccgac aggactataa
     3961 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4021 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4081 cgctgttggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4141 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4201 gtaaggcacg ccttaacgcc actggcagca gccactggta accggattag cagagcgatg
     4261 atggcacaaa cggtgctaca gagttcttga agtagtggcc cgactacggc tacactagaa
     4321 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     4381 gctcttgatc cggcaaacaa accaccgttg gtagcggtgg tttttttgtt tgcaagcagc
     4441 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct actgaaccgc
     4501 gatccccgtc agtttagaag aggaggatgg tgcgatggtc cctccctgaa catcacgtat
     4561 atagttagcc tgacatccaa caaggaggtt tatcgcgaat attcccacaa aaaatctttt
     4621 cctcataact cgatccttat aaaatgaaaa gaatatatgg cgaggtttaa tttatgagct
     4681 taagatacta cataaaaaat attttatttg gcctgtactg cacacttata tatatatacc
     4741 ttataacaaa aaacagcgaa gggtattatt tccttgtgtc agataagatg ctatatgcaa
     4801 tagtgataag cactattcta tgtccatatt caaaatatgc tattgaatac atagctttta
     4861 acttcataaa gaaagatttt ttcgaaagaa gaaaaaacct aaataacgcc cccgtagcaa
     4921 aattaaacct atttatgcta tataatctac tttgtttggt cctagcaatc ccatttggat
     4981 tgctaggact ttttatatca ataaagaata attaaatccc taacacctca tttatagtat
     5041 taagtttatt cttatcaata taggagcata gaattcctgt aacaatagca ataccccaaa
     5101 tacctaatgt agttccagca agcaagctaa aaagtaaagc aacaacataa ctcacccctg
     5161 catctgcagc tttcttctct aatgtaagaa atagtggctt ccagtcacct gtatccttaa
     5221 tttttaggat atcagatacc acatcatatc caaaagaaac atgccccgta atcttcaagt
     5281 acttggcaaa ctgatctaaa tgtttagccc agtcatcata cttcaccgat gccaacgcat
     5341 taaaaatagc atcacgatcg gctttgctga atttcttatt taaaacatcc ttgtattttt
     5401 caaaagcagc gagagcttca ttcacattgc cgattttctt acctttagac ttatcagcaa
     5461 gttcctgtgc cattttcgaa tatttttcac catatttttc agtcagcgtt tgataaaagc
     5521 taactgttgc atcaacagca tccttaatct gtgaattaag gagattattc tgtgcttttt
     5581 tcaaattttc ttcagcttca tgaacacgag cgataccggc attacgatta ttactgacct
     5641 gagaaatagc cttctggatc tgagttatat cagcatttat ccggttaata cgtgtttctg
     5701 atgctgttac ctgtttttgt ttttcttctc taatcttacc ggccccaacc cgtcgtctgg
     5761 ttgcttcaaa aaaaggacgg ttctgaagcg gatcattggc tcttggtgat agttttttga
     5821 ccagctcatc cagttcttta tatttagcgg atgcctgagc cagttcattt cgttttccag
     5881 cgagcgtttt catttctgca tcacgggcat ggatactgga gcttaaacga gaattgagag
     5941 tcttaatctc tccatccatt ttcaccactt cagattgtgc agcagaaagt tttttttggg
     6001 cgatctcaac agctttagct tcttcactca atgcagccag tcgtttctct tcagcttcag
     6061 ccagtttcaa ctggcgttct gtttcagcct tctcccgttc aatctcttta cgtcgttgtt
     6121 ctgcttcctg aaaagccttt tctgctgctt ccgcttcttt acgggctttt tcttctgctt
     6181 tcgcaaggcg caaacgctct gcttccgcct gcatagctgc attattagca tgagcaagct
     6241 ctgttgctga aggcgtacgt gaggcattgt gacgaagagc ctcattcacg atatccttca
     6301 ggcgctgagt cagcgcatcc ctgtttgcct ttgctttcgc ctgtgcttcc gctgcagctt
     6361 ttgcccgggc agcctgctct gcctgtgttt tctttaattg agcagtagac catttagcag
     6421 ttgcatgaat agctgcagaa ctttcacttt tactgcctcc ttttccacct ccgccgccag
     6481 agccactccc gtcaggagta ccattcaaaa gagtaataat tacctgtccc ttatcatcat
     6541 aaggaacacc atctttatag tacgctaccg cggtttccat tataaaatcc tctttgactt
     6601 ttaaaacaat aagttaaaaa taaatactgt acacataacc actggtt
//
