LOCUS       CP077286                1822 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1388 plasmid unnamed5, complete
            sequence.
ACCESSION   CP077286
VERSION     CP077286.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357530
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 769.82x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:23:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,178
            CDSs (total)                      :: 5,048
            Genes (coding)                    :: 4,708
            CDSs (with protein)               :: 4,708
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 340
            CDSs (without protein)            :: 340
            Pseudo Genes (ambiguous residues) :: 0 of 340
            Pseudo Genes (frameshifted)       :: 145 of 340
            Pseudo Genes (incomplete)         :: 190 of 340
            Pseudo Genes (internal stop)      :: 35 of 340
            Pseudo Genes (multiple problems)  :: 27 of 340
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1388"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1388"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            complement(880..1167)
                     /locus_tag="I6L18_25660"
     CDS             complement(880..1167)
                     /locus_tag="I6L18_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA64877.1"
                     /translation="MRIKYESRWKLEELKLEICRSIDSLIDEYHIEYAMGINLYMNCED
                     KEFNPVDIVNEDGKNIMFVVSNNEKIQKPKKSAKKKIPAPVIELDKYRKK"
                     /name="hypothetical protein"
     gene            complement(1180..1713)
                     /locus_tag="I6L18_25665"
     CDS             complement(1180..1713)
                     /locus_tag="I6L18_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="firmicute plasmid replication protein RepL"
                     /protein_id="QXA64876.1"
                     /translation="MLEQQFEDFEQQKRRDHQAQDQSKSAKQKRDRLNPPDWSQVTKAG
                     WNMLDSISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDLES
                     IGAIVKIPVGIYYAYALDPYQVWRGYNKSKKYAAFNAKTLTQKDGVIDRKLRLMLSRRE
                     EFEGSLKTSNHDLD"
                     /name="firmicute plasmid replication protein RepL"
     Typing          378..629
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.885"
                     /coverage="97.0"
                     /evalue="1.85e-99"
ORIGIN
        1 gtatgttggt gtcataatgc tttgattttt aagagaatgt cgatttttgc tctaaagatc
       61 tatcaaagca cttttttgct acacaagatt taattagttc atcgaacatt tggggtgtcg
      121 gggcgaagcc ctgacccagt cacgtagcga tagcggagtg tatactggct tactcttctg
      181 caagcctcgt cagccgtgat tttgcccgat ttcgcgctct ccacgttcaa cagctcgatc
      241 atgtttttgc agtccgttca ccgctaaaac gccccgcatc agtgcgtacg tgctcaatgt
      301 ggtttccggt tctcttccgc ttcctcgccc actcgctcac taacgttcgg tcgctggggc
      361 tgcggcgagc ggtgccacct ctcgcatgtt atcggtgatt ccgctgttgt tctttcgggg
      421 atgagtcgcc agatagctct tcaaacgctc tcagaagctc tcagacgcac gaaacgggtt
      481 ttaaatgccc atcagggcat ggaaggcgcg aaaaacgctt acagagggtt ttaggaggtc
      541 aggcgcgccg atacccctaa ccaggggatt tcaggtcgcc aggaaccgcg tcagccccct
      601 gcaaagccca tttgcacgga ataggcagcc acgcggttgg taggtggtcg tagaccgtcg
      661 atgttacagc gatgggggtc tggggttctc cccagacggt ttccggttgc gctcaaggcg
      721 ctgccggaac cgtggcggct cggtcgttct ggaaaacggg gagcaaaaag acgcaaggga
      781 ttttgaggcg gcaaaagagc aatgcaggat agacaatcaa ctagaaattg catgcaatgt
      841 tctgtggatt gtccgcctgc cgctggctct gtatggccat cactttttcc gatatttatc
      901 taactcaatt acaggtgcag gaattttttt cttcgcactt ttttttggtt tttgtatttt
      961 ttcattgttg ctaacgacaa acataatatt ctttccatct tcattgacaa tatcaacggg
     1021 attaaactcc ttatcttcac aattcatata tagatttatt cccattgcat attcgatatg
     1081 atattcatca attaacgaat ctattgatct acatatttct aattttagtt cttctaattt
     1141 ccaacgtgat tcatatttaa ttctcatagc gcatcatacc tagtccaagt catgattaga
     1201 tgtcttaagc gagccttcaa actcttctct cctagagagc atgagccgca acttgcgatc
     1261 aataacgcca tctttttgag ttaatgtttt tgcgttaaac gctgcatatt ttttagattt
     1321 attatatccg cgccatacct gatacggatc taacgcataa gcataataga tacctactgg
     1381 tattttcacg atggcaccaa tactttctaa atctccaatc caatattgga tcgtccttaa
     1441 actaacaccg aaataatcag ctaagaattt ttggtcacat acaacagccc cacacgattt
     1501 atctatattt tgagcaaaaa aagaatatag cctaaatgct ctgggaacct tctcagatat
     1561 agagtccaac atattccagc ccgccttagt aacctgcgac cagtcaggtg gattcaaccg
     1621 atctcgtttt tgcttagcac ttttggattg atcttgtgcc tgatgatctc gccgtttttg
     1681 ctgttcgaaa tcttcaaatt gctgttccag catacttgca tccttacctg caacgttgtt
     1741 tcgtacctta cgaaatttat atcgtacatt acgaaacaac acacgcaaac atgtttgcgt
     1801 atacacatgc aaataacgtt tc
//
