LOCUS       CP077285                4082 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1388 plasmid unnamed4, complete
            sequence.
ACCESSION   CP077285
VERSION     CP077285.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357530
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 769.82x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:23:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,178
            CDSs (total)                      :: 5,048
            Genes (coding)                    :: 4,708
            CDSs (with protein)               :: 4,708
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 340
            CDSs (without protein)            :: 340
            Pseudo Genes (ambiguous residues) :: 0 of 340
            Pseudo Genes (frameshifted)       :: 145 of 340
            Pseudo Genes (incomplete)         :: 190 of 340
            Pseudo Genes (internal stop)      :: 35 of 340
            Pseudo Genes (multiple problems)  :: 27 of 340
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1388"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1388"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            join(3715..4082,1..1792)
                     /locus_tag="I6L18_25645"
     CDS             join(3715..4082,1..1792)
                     /locus_tag="I6L18_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QXA64875.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            complement(2048..2749)
                     /locus_tag="I6L18_25650"
     CDS             complement(2048..2749)
                     /locus_tag="I6L18_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QXA64873.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3282..3536)
                     /locus_tag="I6L18_25655"
     CDS             complement(3282..3536)
                     /locus_tag="I6L18_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QXA64874.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     Typing          complement(3538..3714)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3715..4073
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2049..2749)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..1789
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gttccatcac atggataaaa caaatcccca tgcgcacatc atgctgacca cgagagctgt
       61 cggacctgct ggattcggtg gaaaggtcag ggactggaac gaccggacac atgccgagac
      121 gtggcgtgca tcatgggctg accatgcgaa cagggcactg gcgaacgccg gttatcagga
      181 agagatagac catcgttcat acgagcgaca ggggcttgag aaaacgcctg gtattcacct
      241 cggaaagtct gcctgtgcga tggaaacacg cggtatcgaa acagaacgcg gcgaacagaa
      301 tcgtctgatt aacagactta acctggaaat acagatttcc cgtaccgaac tcagaaacag
      361 cgggctgaca gagcctgccc gccgtgtggc tgaccttctg aatatcgtgc ttcctgacaa
      421 cgcaacttcg tacacactac gtgacatcat cgaagcgttg ccgaaggaca gtaatgcggc
      481 atggaaactc accagtaatg ccatgtcgat gatggcagat atgcaggcga cgcgcagacg
      541 ctgggaagag ctgaacaccg aacgaaagga agcgatgagc catgctgcca gcctgaaaaa
      601 atcatcgccg ttttcgtccg gtttttcccg tattcccctg atgcaatggg cggcaccgga
      661 ataccgtagc gaacagggaa aaatccagtc gctgagccag caaatggaaa aattgcgaca
      721 gcattaccgg aaggtggaga aacaggacat tcccgcgagt cagacggcat ttgaaaaaca
      781 gtggcagcag tggggggcat ccggccttgc agctctcaga gtccagttgc ttaaacgtga
      841 agaggaattg cgccggaagg agcaggagga tacagaacgt agtcgcgcgg agcgacgagc
      901 ggaacagctc aggaaaaatg acaatgccgt tatggattca ggggtactga gcgcagtcgt
      961 tacggggtat ggcgaggccc ccatgcctgg taacggggaa atgacctgtt accttctgct
     1021 tcacaaccgg aacggagaat acacgctctg ggggaacgag ctggagaaat accgcaccag
     1081 aatctttgag cgtgttgatt taatgcgtga caggagtggt tatatctgtg accgaagcga
     1141 aatcggtcaa catccaccac tgcaacgtgt ttattcctct gcgacctttg agcaacttct
     1201 gacgcaggtt tgccagacat ggccgcaata tacccgcaat ctccgccagc cgaagacatg
     1261 gccggagtct ttctgtcttg gagaagaccg ccagccagcc atgccatcac tggcagcaag
     1321 gaaggtggat tttacgcagg gacgtctgct accgacgctg atgccggtaa tgagttctgt
     1381 cgaccgggaa acccgccagc ttcagctttt gctggtcatg ggcgtggatg actctctggg
     1441 cggtgttgtc cggcttaatg gtacgctgta tcccgcgttt gccgttccct cagccgataa
     1501 cagccagctg gtcatcagtg cactgaccga taaggggtta cggtatgcag ggtatggtgt
     1561 tgccgtaaat cacgatgcag acagccacat atcacctgcg ccggaactta tggagtttca
     1621 tctgaaaacc cgtgaggcac cgctttttgc tgccgtcaac acaccagaaa aacagccgga
     1681 tcatctgttc cgctcgctgg gttttaatcg tacctgggac gaatggcggc gcgaggaaga
     1741 tgcacgaaca cacgccaccg agaggagaca tgatcgcggt tggtcacagt gaatgtgaac
     1801 aatattttgt tttgtttaac atcgtgttat gttcttcgat gttcttcgat gttcatcgac
     1861 agggatgaat gcaagaagca cacacataat gcaagcatag ttttgttttt ccgcccccaa
     1921 aaaatggggg ttcttttttc gctgcattag agcgcttttg acgcaatttt tcacttcaat
     1981 agtaccttcg cactactaac acaaaaaaaa cgcagtaggg agcgttttag gtgccttaaa
     2041 atcgatttta gatcgtcatc ttgatttgtt tgtcttcctt gaagcgaaaa tgcagggcta
     2101 caaccgtgcg cccctttttg atcgtctcaa ccgttacagc caggtcgctt tttttgttca
     2161 gttcgtcaat gcagggtctt aataattgct ggttaagtga tttatatgtt tgatatttat
     2221 gctctatatc aagcattgag cgaaaatcat cgagagcgat aatacgttcg ccagtactgc
     2281 ggaactgctg gaggagttcg tatatcctta tactgtaact tcttgataat ctagatatat
     2341 tcttgattac aactctagta aattgccctt gtagttgtgt caaataaggc attactgcgt
     2401 cagaaaatgt tatttgtgct ctggcctcgc ctttagcgta ctgtgcccga tactggatcc
     2461 agcggaactc ctcacgtttc tcgtcatcct tgagaatgat tgacctgtcc cacaaacgat
     2521 caattgcttc ctgtaactga acctcggcat ttttccgccc catgtcaggg aatgaatcga
     2581 aatattccgc agctgtaatg gtcattgtct tttgcaattt tggagattct gcatcagccc
     2641 cagacttcaa tcgaccgatg caaagtaaca aaaatcgctg ttcctgtagg gtcaatttat
     2701 aactggcttc aaccagcgca ttggatttat aggcaattag atccgacata gagcatccct
     2761 ccccaaaagg taatgaataa aagataaaca taccctcaac gaaaggcaat gcctttttat
     2821 tttaatatcc ctttttctgt gaatattacc ttaaatcata ttgcctttac cttaaattcc
     2881 tccacctaaa caccttaaat catattacct ttaccttaaa tcatattacc tatagcgttg
     2941 taacattttg ttttctataa ggaattttgg cgctaaaaca agtattaaaa acaagatctt
     3001 aaaaacaaga tctaaaaact taacgacgag cctgtggata acttggagtg ctgaaaacaa
     3061 aaaaccaata aaaacaatgc gttgcaaaaa agcagaattg caagttccga atacaccccc
     3121 tccaaccctg tggaaatcca gtattcatgc gggttcagcg catatcacct cccgaaaaac
     3181 cgtgcgtatg tgctcaatca ggcgacggat tcacttccgc ttcctcgccc actcgctcgc
     3241 taacgctcgg tcgctgggac tgcggcgagc ggacgataca atcactgtcc tggcagcgct
     3301 tccagctcgt taatcagcgg cagtatgcgc gtcacatcag cctgtcgtgt cacctctgcc
     3361 ttcaggaact ggagtaacca ggcacgcaac tgcacctcct ccggctttct gacctttgcc
     3421 agcaatgctg cacccacaat gaccttctgc gctgtgtcca gactcctgct cttcgccttc
     3481 aggcgctgta atctggcctc agcatcagca atcttctgtt cgagtgttct gctcatgatg
     3541 taactccgtg cgcgatgaaa aatcgcattt tagcgcgtca ctggtagttt aaaaactgag
     3601 ctggcataat gcacggaaaa tcacgaagtg cgcacttata caatctccac tccgtttcga
     3661 ttgtgtgcgc cctgcggggc tgaaagaaaa ccacaaaaag gcattacggc agaaatggcg
     3721 atttatcatc tcagcatgaa aatcatctcc agaaacagcg gctacagcgc cgttgcttct
     3781 gctgcctatc gttccggttc actgatgctt gatgagcgta ccggactgac tcacgactac
     3841 acccgcaaaa gcggtgtggc tgaagctgtg attctgactc ccgcgacagc ccctgcctgg
     3901 tgtacgaacc gtgccgaact ctggaatgcc gttgagaagg ccgaacgccg gaagaactca
     3961 caacttgcgc gggaaattga actcgccatc ccccgcgaac taccgcagga tgccgcacgg
     4021 gaaaccgttc tcgcctttgt cagagaaaac tttgtcagtc agggcatgat tgctgatgtg
     4081 gc
//
