LOCUS       CP077284                2101 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1388 plasmid unnamed3, complete
            sequence.
ACCESSION   CP077284
VERSION     CP077284.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357530
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 769.82x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:23:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,178
            CDSs (total)                      :: 5,048
            Genes (coding)                    :: 4,708
            CDSs (with protein)               :: 4,708
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 340
            CDSs (without protein)            :: 340
            Pseudo Genes (ambiguous residues) :: 0 of 340
            Pseudo Genes (frameshifted)       :: 145 of 340
            Pseudo Genes (incomplete)         :: 190 of 340
            Pseudo Genes (internal stop)      :: 35 of 340
            Pseudo Genes (multiple problems)  :: 27 of 340
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1388"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1388"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            join(1506..2101,1..370)
                     /locus_tag="I6L18_25635"
     CDS             join(1506..2101,1..370)
                     /locus_tag="I6L18_25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QXA64871.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            374..640
                     /locus_tag="I6L18_25640"
     CDS             374..640
                     /locus_tag="I6L18_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA64872.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          1900..2101
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="87.0"
                     /evalue="9.74e-102"
ORIGIN
        1 gcgggtgaac tatgagccga atatcgatat tcgggcagta aaaactaaga caggtgaggt
       61 tgtggccaac gttgccgagc aactgcaaag cgcggttgct gaaacgctga aatactccgt
      121 taaaccggaa gatatggcaa acgatcctga gtggtttctt gagctgacgc ggcagcttca
      181 caagcgccgt tttatctcga ccggtggggc gctaaaaaac gtcctccagt tggatcgaga
      241 aaccaatgag gatcttgtca ttgccgacga tgtaggggat ggcactgatg acgggaagcg
      301 gacggcgttt gtctgggatt caggtaaacg gcgttacaaa cgcgcccctg agaaggataa
      361 atcggattaa cgtatgaata ttaatattga atacctgaat ggaaataaga ctattggttt
      421 atttttttta agaagtgaag cggtgattcc tgacaggttt aaaaacctta ttttgcttat
      481 tgatggatta agttttggca catttggttt tcatccgcac gaaggttttg aggatgaatt
      541 aattttatat attcagaaaa caaacgagag ggtaaaaact ctttttgtga aaatagattt
      601 aaacggtatc aagtttggat ttttaagaac gcattcttag ttctggaaaa gagccagcgg
      661 caggctgagg tgataggtac gagattgcat gcaatctcta gtgctctgtc tatcctgcat
      721 tatcctcagc attatcctca gcattatcct cagccttgcc aactcgacac caatgcagga
      781 tagacaatcc gatgtcaaat gttaacactc tgcgagtggt acattttccc cggattatcg
      841 tcctgagcct gccgctggct ctctttctac cgcctcgctt tgctcgttgc tcaacgcctc
      901 acagacacgg attaaaatcc gcatccgttc accgtttttt aaagtccgtt aaaagcatga
      961 tgccatctcc gagagttaat ctcgtcaaat gctaaatcgt gggggtcccc tttggggttc
     1021 cgatttagtg attgacgaca ccaccgatta aaaaacttat gcggggtgga tggtttcacg
     1081 aagtgaggcc atccacctgt aagacagggt tttgttttta ttccctgttt tggtgatcgg
     1141 gtgtgtggaa aaggttgggg taagccgttc gggggtgctt gttttggggg gttaaaattg
     1201 tggttatttt ttgcgcaatt ctcgcgcgtg atccttgtat ttatacttaa gggataaatg
     1261 gcggatatga aatagtggtt tagcccagta atgacgaggc tttgagtggg ttttgacagg
     1321 tcaaagaaaa tggagcagaa ttgaggcgtt tttaatcggc gttggggagt gcgtcaacac
     1381 tccccaacat ttcgaatgtg tcacctcagc ggcaaactct ggtgacatgt actggctcgc
     1441 aatgcacagg tacgtgatga atataccaca tcaaatcaca gcctgcccag atcggagcag
     1501 gcttaatgtc agaagataaa ttcctttcgg actacagccc ccgtgatgca gtttgggata
     1561 cccagcgcac gcttaccgat tctgtcgggg gtatctacca gactgctgct gaattcgagc
     1621 gctatgcact ccgtatggcc tcctgtagcg gtttgttacg ttttggttgg tctaccatca
     1681 tggaaaccgg agaaacgcgc ctacggcttc gtagtgcgca attttgccgt gtccgtcatt
     1741 gccctgtctg ccagtggaga agaaccctca tgtggcaagc ccgtttttat caggctctac
     1801 cgaaaattgt tgtggattac ccgtcttccc gatggttgtt tctgacgtta actgtcagga
     1861 actgcgagat aggtgaactt ggaacagtcc ttacagcaat gaatgcggcg tttaagcgaa
     1921 tggaaaagcg aaaggagcta tcacctgttc aggggtggat cagggctacg gaggtgacgc
     1981 gaggtaagga tggcagcgca catccgcatt ttcactgtct gctgatggtg caaccttctt
     2041 ggtttaaagg gaagaactac gttaagcacg aacgttgggt agaactctgg cgcgattgct
     2101 t
//
