LOCUS       CP077278                2089 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1385 plasmid unnamed5, complete
            sequence.
ACCESSION   CP077278
VERSION     CP077278.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357527
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 839.78x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 17:18:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,206
            CDSs (total)                      :: 5,077
            Genes (coding)                    :: 4,847
            CDSs (with protein)               :: 4,847
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 92 of 230
            Pseudo Genes (incomplete)         :: 142 of 230
            Pseudo Genes (internal stop)      :: 28 of 230
            Pseudo Genes (multiple problems)  :: 28 of 230
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1385"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1385"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            join(1293..2089,1..169)
                     /locus_tag="I6L15_26025"
     CDS             join(1293..2089,1..169)
                     /locus_tag="I6L15_26025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QXA90901.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            173..439
                     /locus_tag="I6L15_26030"
     CDS             173..439
                     /locus_tag="I6L15_26030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA90902.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          1687..1918
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 cggtggggcg ctaaaaaacg tcctccagtt ggatcgagaa accaatgagg atcttgtcat
       61 tgccgacgat gtaggggatg gcactgatga cgggaagcgg acggcgtttg tctgggattc
      121 aggtaaacgg cgttacaaac gcgcccctga gaaggataaa tcggattaac gtatgaatat
      181 taatattgaa tacctgaatg gaaataagac tattggttta ttttttttaa gaagtgaagc
      241 ggtgattcct gacaggttta aaaaccttat tttgcttatt gatggattaa gttttggcac
      301 atttggtttt catccgcacg aaggttttga ggatgaatta attttatata ttcagaaaac
      361 aaacgagagg gtaaaaactc tttttgtgaa aatagattta aacggtatca agtttggatt
      421 tttaagaacg cattcttagt tctggaaaag agccagcggc aggctgaggt gataggtacg
      481 agattgcatg caatctctag tgctctgtct atcctgcatt atcctcagca ttatcctcag
      541 ccttgccaac tcgacaccaa tgcaggatag acaatccgat gtcaaatgtt aacactctgc
      601 gagtggtaca ttttccccgg attatcgtcc tgagcctgcc gctggctctc tttctaccgc
      661 ctcgctttgc tcgttgctca acgcctcaca gacacggatt aaaatccgca tccgttcacc
      721 gttttttaaa gtccgttaaa agcatgatgc catctccgag agttaatctc gtcaaatgct
      781 aaatcgtggg ggtccccttt ggggttccga tttagtgatt gacgacacca ccgattaaaa
      841 aacttatgcg gggtggatgg tttcacgaag tgaggccatc cacctgtaag acagggtttt
      901 gtttttattc cctgttttgg tgatcgggtg tgtggaaaag gttggggtaa gccgttcggg
      961 ggtgcttgtt ttggggggtt aaaattgtgg ttattttttg cgcaattctc gcgcgtgatc
     1021 cttgtattta tacttaaggg ataaatggcg gatatgaaat agtggtttag cccagtaatg
     1081 acgaggcttt gagtgggttt tgacaggtca aagaaaatgg agcagaattg aggcgttttt
     1141 aatcggcgtt ggggagtgcg tcaacactcc ccaacatttc gaatgtgtca cctcagcggc
     1201 aaactctggt gacatgtact ggctcgcaat gcacaggtac gtgatgaata taccacatca
     1261 aatcacagcc tgcccagatc ggagcaggct taatgtcaga agataaattc ctttcggact
     1321 acagcccccg tgatgcagtt tgggataccc agcgcacgct taccgattct gtcgggggta
     1381 tctaccagac tgctgctgaa ttcgagcgct atgcactccg tatggcctcc tgtagcggtt
     1441 tgttacgttt tggttggtct accatcatgg aaaccggaga aacgcgccta cggcttcgta
     1501 gtgcgcaatt ttgccgtgtc cgtcattgcc ctgtctgcca gtggagaaga accctcatgt
     1561 ggcaagcccg tttttatcag gctctaccga aaatcgttgt ggattacccg tcttcccgat
     1621 ggttgtttct gacgttaact gtcaggaact gcgagatagg tgaacttgga acagtcctta
     1681 cagcaatgaa tgcggcgttt aagcgaatgg aaaagcgaaa ggagctatca cctgttcagg
     1741 ggtggatcag ggctacggag gtgacgcgag gtaaggatgg cagcgcacat ccgcattttc
     1801 actgtctgct gatggtgcaa ccttcttggt ttaaagggaa gaactacgtt aagcacgaac
     1861 gttgggtaga actctggcgc gattgcttgc gggtgaacta tgagccgaat atcgatattc
     1921 gggcagtaaa aactaagaca ggtgaggttg tggccaacgt tgccgagcaa ctgcaaagcg
     1981 cggttgctga aacgctgaaa tactccgtta aaccggaaga tatggcaaac gatcctgagt
     2041 ggtttcttga gctgacgcgg cagcttcaca agcgccgttt tatctcgac
//
