LOCUS       CP077277                2744 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1385 plasmid unnamed4, complete
            sequence.
ACCESSION   CP077277
VERSION     CP077277.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357527
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 839.78x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 17:18:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,206
            CDSs (total)                      :: 5,077
            Genes (coding)                    :: 4,847
            CDSs (with protein)               :: 4,847
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 92 of 230
            Pseudo Genes (incomplete)         :: 142 of 230
            Pseudo Genes (internal stop)      :: 28 of 230
            Pseudo Genes (multiple problems)  :: 28 of 230
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1385"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1385"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            92..1090
                     /locus_tag="I6L15_26020"
     CDS             92..1090
                     /locus_tag="I6L15_26020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QXA90900.1"
                     /translation="MKNPELKNLTDYSPADKPWDEHRSFSDDVGGIYLRAAGFEHYGER
                     VGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPKIV
                     TEHPGARWLFLTLTVRNCSIDALGETLTAMNAGFKRLKERKEFRHVLGWVRTTEVTRGK
                     DGTAHPHFHTLLMVPPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRKPKDD
                     ESLPCVVAEQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGALKNVLQ
                     LERETNEDLIMADDMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRQLSGED"
                     /name="protein rep"
     Typing          1722..1797
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.593"
                     /coverage="88.0"
                     /evalue="2.64e-23"
     Typing          complement(808..999)
                     /name="Col(MP18)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.482"
                     /coverage="100.0"
                     /evalue="1.32e-94"
ORIGIN
        1 agaacatgaa ctgatttgac gacaattggt acaggtgata caggaatgat aacacgcaat
       61 ttcgtctgtc gctcgcaagg ggacggcaga catgaaaaat cctgaactta agaatctgac
      121 ggattactct ccagcagata aaccgtggga tgaacaccga agcttttccg acgatgtggg
      181 cggtatttat ctgcgtgctg ccggatttga gcactacggc gaacgtgtcg gggcttgtag
      241 tggtattttg cggtttgggt ggtcaacaaa cacagagacc ggagaaacgc gcctgcggtt
      301 gcgtgatgca cggttctgtc gcgtccggca ctgtccgatc tgtcagtgga ggcgttccat
      361 gatgtggcaa gcccgtttct atcagtccct gccaaagatt gtgacggaac atcccggcgc
      421 gcgctggttg tttctcacac tgacagtgcg caactgttcc attgacgcgc ttggtgagac
      481 gctgaccgcg atgaatgcgg ggtttaagcg gttgaaagag cgtaaggaat ttcgccatgt
      541 gttgggttgg gttcgtacca cggaagtaac gcgcggcaag gatggtactg cacatcccca
      601 ttttcacact ctgctgatgg ttcccccttc gatgttgtcc ggccgtgagt atgtgaaaca
      661 tgcccgttgg gtggaactgt ggcgtgattg tctgcgggtg gcgtatgacc cgaatgtgga
      721 tatcagagtg gtgaaatcac ggaaaccaaa agacgatgaa tctttacctt gtgttgttgc
      781 ggaacagctg cgcggcgcgg tggttgaaac tctgaaatat tccacaaagc ctgctgatat
      841 gctggctgat cctgaatggt tcctcgaatt aacacggcag gtacacaagc gtcgttttgt
      901 tgctacgggg ggagcgctta agaacgttct gcaactggag cgggaaacga acgaggattt
      961 aatcatggcg gatgacatgg cagagggtac agatgacggt tcccgtctgg cgttttcgtg
     1021 ggtgccggtt gtcaaaaaat accagcgcga tcgggcgaaa gataaacgtc agctaagtgg
     1081 cgaggattga gcgcgtttca ggcgcgaact attcatgcac tgggagaata ttcagaaacg
     1141 cgctcagaag cgttctaacg tgattttcag aaacgagcaa ggatttactc gttttttgtt
     1201 ttgacgcact cagaagcgtt ttaagcgcgt ttccgggtct ctgctacggc agtccacggc
     1261 tgacagcttt tcgctcacca gaaacacgca tgtcatgttt agcgcggtgt gattctgctg
     1321 tccagtagtc ggaggggggg ggcgtgtttc tacaggtctt cgtgggttga tgatagtgga
     1381 aatgcgaaag aaacacgctc atccgttgct taacacaaaa gatacaaagc aacaacattt
     1441 ccgcacatgg acaggaaggg gaagtgagcg catttgaggg cggggtgtaa caatccccat
     1501 ttgacgcaca gtgaatttta acccctcaga cgcgctcaga cgcgttctaa cgtgattttt
     1561 gcgggcaggc aaggttttgt tcgttttttc gcgtttgatg cgttcagggg cgttttaagc
     1621 gcgtttctgt cgcatcccgg cgctaatacc atccccatgg ttaatgggag cttcagaaac
     1681 gcgcagatgt cgttttaacg tgtcccgggc aggttgtcga gggtgtcggg gcgtagccct
     1741 gaccaggtgg taatcgtatc ggcgtgcgcg gttatacgat tacacatcct gtcccgtcat
     1801 cggagttgtt tttgcggtgt tcctgcggca aattgagcgg gcatagcaac acctcaaaac
     1861 aactatcatc aggaaattga ctttgacggc cccctgacac agggggattt tttttgcctg
     1921 ttatctgcgg ttttcaccgc gtcgcagacc gcacacaatc gaaacgaagt tgagattgta
     1981 taagtgcgca cttcgtgcac ttcgtgagat tccccccgac tgccagtttg tcgatcgcgc
     2041 ggaacggctg cggcacgaaa acggcaaaat gatttgccgt ccggttgcca gatctgcgcg
     2101 cttccgtgga gggcgacacg gcgcaaaaac tgccgatatt gcgccagcgt tccggtaaca
     2161 caaactcgca gaaatcggga caggatgtgg gaatgtcgga ccgcgcacgc gcactcagac
     2221 attccccacc tggtcagggc tacgccccga caccctgcca acgcttcgcg gcacgtgaaa
     2281 aaattccgcg tactgctcac cgttcccgaa ttcgtcaaac agaaaatcgt ggggtcccgc
     2341 gcctccggcg tgggttccga ttttgtgatt gacgatggca cggaaaaaac actggaggag
     2401 gtggttggtg tcacggagtg atgccaacca cctgcaagac ggtttttgtt gttcacaaaa
     2461 ttggtggaaa agcctgtggg tgttttgtcg ggaaaacgct ttcgtggttg ttgtttttgc
     2521 cctcaaaaaa tcgaacaaaa aacaggcgat tttgctgctg ctttcgtgcg cgttccttgt
     2581 atttatactt aagggataaa tgcgattttt tgaatcagct ctatcccttg ccatatctgg
     2641 cctgagcgcg gatttgcgtg taaataggtt tgagagtatt ttgtcgggag tggttataaa
     2701 ttagcccctg cgagtcttcc accactccag gggcgcgacc acct
//
