LOCUS       CP077276                3182 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1385 plasmid unnamed3, complete
            sequence.
ACCESSION   CP077276
VERSION     CP077276.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357527
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 839.78x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 17:18:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,206
            CDSs (total)                      :: 5,077
            Genes (coding)                    :: 4,847
            CDSs (with protein)               :: 4,847
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 92 of 230
            Pseudo Genes (incomplete)         :: 142 of 230
            Pseudo Genes (internal stop)      :: 28 of 230
            Pseudo Genes (multiple problems)  :: 28 of 230
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1385"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1385"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            join(2819..3182,1..104)
                     /locus_tag="I6L15_26005"
     CDS             join(2819..3182,1..104)
                     /locus_tag="I6L15_26005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QXA90897.1"
                     /translation="MSDTNKFRSLRLARAWSQEQLAELSGMSVRTVQRIENGDQPSLET
                     LSALAAVFEVSVADLSGPDAHGDDALDQKIAEAKSKIAEEGRFYRSVITAVVVCALLLV
                     LNHFTAPASAWSFWVAGIWCALLVIRGMRTFVLRGLISRWQQKRLRRMLRR"
                     /name="helix-turn-helix domain-containing protein"
     gene            116..616
                     /locus_tag="I6L15_26010"
     CDS             116..616
                     /locus_tag="I6L15_26010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA90899.1"
                     /translation="MDIADFLRNTPVWVWILLAFLLRRGFAALYDREMTTGRLFFLPIL
                     FLVWGAYGVITETKLAGVSLTMMTAGLLVGTALGYRLWRSQPPLRNSDNPGMIIRAGTP
                     LTLGLIVITFCMKFILVSVIDLHPGLRSSAIFCMLFGGVTGLVDGVFWGGTLRLFIPWY
                     SKR"
                     /name="hypothetical protein"
     gene            complement(972..1085)
                     /locus_tag="I6L15_26015"
     CDS             complement(972..1085)
                     /locus_tag="I6L15_26015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QXA90898.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGN"
                     /name="replication initiation protein"
     Typing          complement(973..1457)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.391"
                     /coverage="93.0"
                     /evalue="1.66e-162"
ORIGIN
        1 tctggtgtgc gctgctggtt attcgtggca tgagaacgtt cgttttgcgc gggctgatca
       61 gcagatggca acagaaacga ctgaggcgga tgctgcggcg ataatgggaa tacttatgga
      121 tattgcagac tttttacgaa atacccccgt ctgggtatgg atcctgctgg cttttttact
      181 acgacgcggt tttgctgctc tttatgaccg ggagatgaca accgggcgtt tgttttttct
      241 cccgatactt ttccttgtat ggggagcata tggtgttatc actgagacaa aacttgctgg
      301 cgtaagcctt acaatgatga cggcaggact gctcgtaggc accgctcttg gatacaggtt
      361 gtggcgctca cagcccccgc tgagaaattc agataatccg ggcatgatta tcagggccgg
      421 gacaccgctg actttgggac tgattgtaat tactttttgc atgaagttta tacttgtctc
      481 agtcattgat ctgcaccccg gattacggtc atccgcaatt ttttgcatgt tatttggcgg
      541 tgtgacagga cttgttgatg gcgtattctg gggtggaacc ctgagacttt tcataccctg
      601 gtacagcaaa agatgattgt tagtaggtgt atgtatttat gggtttttat ggggtttcat
      661 cggccatccc aaaaccctga ttttacgatc tatgaaatag gccggtttaa tcttcaaacg
      721 ccttcatgga tgaagacgat aatcgctcct gtaaaaagga tcttcttgag atcctttttt
      781 tctgcgcgta atctcttgct ctgaaaacga aaaaaccacc tggggaggtg gtttgatcga
      841 aggttaggtc agttggggaa ctgcttaacc tggtaactgt cttgtgcgag acgagtcacc
      901 taaactgtcc ttccagtgta gccgcactca ggcccccact tcaagaactc cagataaatc
      961 tctcgcacat ctcagttacc aatggctacc gccactggcg atttgtcgtg tctttccggg
     1021 ttggactcaa gttgatagtt accggaacgg gcgtggtagt cggactgaac ggggggttcg
     1081 tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg
     1141 agacaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga
     1201 gcgcacgagg gagctaccag ggggaaacgc ctgggatctt tatagtcctg tcgggtttcg
     1261 ccgccactga tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa
     1321 aaacggctac gccgcatcct ttttccggtc cttcctgatt tgagcactgc ctgcgtatcc
     1381 cgttatttgt gagccggtaa cgcccgccgc agtctcacgg caggagcgta gcgaccgagt
     1441 gagcgaggag gcgtcatttc acctttacct acatgttgca cttacgcacg atagccacat
     1501 ttttcctttg ccacatgaag cacttccctg aattaccact ccgatttaac atattaagcc
     1561 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgctaaa
     1621 acccgctgac gcgccctgac gggcttgtcg gctcccggca tccgctcaca ggcaagctgt
     1681 gtccgtctcc gggagctgcc tgtatcagcc cgttatgcca tcatcgccga agcgttcccg
     1741 gcaacgttgg tagcgttcat ccgccacttc gtgaagttgt tctggtgaaa aaaacacgta
     1801 caggacgggg caggatgtgc atttgtcgga ccgcgcacgc gcaatcagac aaatgccacc
     1861 tggtcagggc acagccccga caccctggac aacaccaaag caataagata aaacgtacct
     1921 gggcgcgttt ctctgcctct tttttcatca tctctgggat tcgcgctcac ccgtcctctg
     1981 ctgtcgggtt tatgtatccc cgtcgcccgt ggagatgaat aacgggccgg gggattaaaa
     2041 atccccgtgc cgcacagcca gcgttgcata aaattccgct ctgcggtgac tattgtttaa
     2101 tcaaaaatcc ccgctgttcc tccagaaaaa cgcgagtctg ttctcattcc caggatgccc
     2161 acaccccacg caaaactaac ttttttctga tttgtttttt aaatcatgtt gttatggttt
     2221 gaaaaatata ttttcttatt cggatttata caaaagcggc tcgtcggcga ctactggatg
     2281 actcggcaac gagtcgtcgg tgatttatcg atgactcaac acactgtcgc tctggtgttc
     2341 agagaaggcg agtcgccaga gagtcgcgag gaaaaatact ggatgactcg ccgacgactc
     2401 ccgtagtgaa tcgctcacga ctcgtctgta actacagtct gatttcttct tatcagggct
     2461 gaaaacgtcc gaaaatacgc cttatgggca tcagacgcgc ccaaaccgat cgccattcag
     2521 tttcatgcat agtcatgcaa cgcgctgaaa gcgttcctga cgcatttttc cggtttaccc
     2581 gggggaaaac atccattttt acggtttttg cgccagaagc gcgctgagcg cgttttaacg
     2641 gtgcgtacaa tgaactctta tgttaaatct gaccgtacat gcttctgaag ttacggtttc
     2701 aaagacctca atgcggcatg tcagaccgct gtcacgttac tgtcgggtta agatcgtggt
     2761 cgcgcaggat tacgcggtca acactgtgtg ctgaccatct tccattcgga gacaggaaat
     2821 gagtgatacc aacaaattca ggtcgctgcg tcttgcgcgt gcctggtccc aggagcagct
     2881 tgctgaactg tccgggatga gcgtgcgcac ggttcagcgt atcgagaacg gcgatcagcc
     2941 aagcctggaa acactgagtg ccctggctgc tgtatttgag gtgagcgtgg cggatctttc
     3001 cggccctgat gcgcatggcg atgacgctct tgaccagaaa attgccgaag cgaagagcaa
     3061 aatagctgag gaaggccgtt tttaccgatc agtaattacg gctgtcgtcg tctgcgcgct
     3121 gttgcttgtt cttaaccact ttacggcccc ggccagcgca tggtcatttt gggttgcggg
     3181 ga
//
