LOCUS       CP077275               12821 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1385 plasmid unnamed2, complete
            sequence.
ACCESSION   CP077275
VERSION     CP077275.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357527
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12821)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12821)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 839.78x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 17:18:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,206
            CDSs (total)                      :: 5,077
            Genes (coding)                    :: 4,847
            CDSs (with protein)               :: 4,847
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 92 of 230
            Pseudo Genes (incomplete)         :: 142 of 230
            Pseudo Genes (internal stop)      :: 28 of 230
            Pseudo Genes (multiple problems)  :: 28 of 230
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1385"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1385"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            complement(join(12768..12821,1..1077))
                     /locus_tag="I6L15_25915"
     CDS             complement(join(12768..12821,1..1077))
                     /locus_tag="I6L15_25915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="QXA90888.1"
                     /translation="MTVKNNYLNRGYLVTSTTQAKKIAKLHLEKLDLADKFSFGLPEVD
                     DRYHVWRVPLVATGHRIGEIVINSKTSTIDYKKSSKSPFLIKKAMEVVHTPKKVKKEKK
                     IISKSPLNNMLLQGNCAETLKKLPDESVNLVFTSPPYYNAKPEYSEYHTYDEYLSLLRS
                     VIKECHRVLSEGRFFVINVSPVLIRRASRNEASKRIAVPFDLHRLFIEEGYEFIDDIHW
                     VKPEGAGWALGRGRRFAADRNPLQYKPVPVTEYILVYRKKTDKLIDWNIRNHHSKEDVF
                     DSKIGDDYEKTNLWKINPSRNRKHPATFPYGLAERVIKYYSFKNDVILDPFAGSGTTAK
                     AAIDLGRRFVMCEISKQYIDLIIEGTMLGGDLKIIN"
                     /name="site-specific DNA-methyltransferase"
     gene            1154..2221
                     /locus_tag="I6L15_25920"
     CDS             1154..2221
                     /locus_tag="I6L15_25920"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfrBI family restriction endonuclease"
                     /protein_id="QXA90889.1"
                     /translation="MNFKDKNCFPNELIALAKISKNDVLDKFGTDVFKKVVYDVLTGKN
                     VREFTEILTRTRLLESNLSFFDFFVDKMKEGITPKQLYLYAKNALSNKSYVKSNQPVLE
                     WMVMMTNKQTQNVLRDEHGDGFDRLALRTQEEILKIKNGYEDKIGEISIGGQKVSLEDF
                     CYIILSLGSQTLTIRGSEKSLHGKYFEKLILGSLFTIMGFEYKEKIEEGLNAKCFTLST
                     RADDRESDATLVFNGKAIRVDIGFIGRGNTEISLDKVSRFRRMDDIGGVMHNISTMVIV
                     DVIGDRSRIVNMAEEIDGKVVAMSDPYWVAKVSSYISSKLNVDDLLEDKPQLKDIQSFI
                     SDALENVDLEKYIKL"
                     /name="CfrBI family restriction endonuclease"
     gene            complement(3233..3424)
                     /locus_tag="I6L15_25925"
     CDS             complement(3233..3424)
                     /locus_tag="I6L15_25925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QXA90890.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDVDDQADICEALHDHA
                     DELYRSCLAHFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3492..3815
                     /locus_tag="I6L15_25930"
     CDS             3492..3815
                     /locus_tag="I6L15_25930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QXA90891.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3778..4521
                     /locus_tag="I6L15_25935"
                     /pseudo=""
     CDS             3778..4521
                     /locus_tag="I6L15_25935"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4493..5053
                     /locus_tag="I6L15_25940"
     CDS             4493..5053
                     /locus_tag="I6L15_25940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QXA90892.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            5060..5293
                     /locus_tag="I6L15_25945"
     CDS             5060..5293
                     /locus_tag="I6L15_25945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QXA90893.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5777..6199)
                     /locus_tag="I6L15_25950"
     CDS             complement(5777..6199)
                     /locus_tag="I6L15_25950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QXA90894.1"
                     /translation="MRSAATRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRAKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSLKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            6321..6599
                     /locus_tag="I6L15_25955"
     CDS             6321..6599
                     /locus_tag="I6L15_25955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA90895.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(6591..6881)
                     /locus_tag="I6L15_25960"
     CDS             complement(6591..6881)
                     /locus_tag="I6L15_25960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA90881.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPST"
                     /name="hypothetical protein"
     gene            7120..8619
                     /gene="csa"
                     /locus_tag="I6L15_25965"
     CDS             7120..8619
                     /gene="csa"
                     /locus_tag="I6L15_25965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="QXA90882.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGREHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     DQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGDAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANVWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNKEIIKPAH"
                     /name="csa"
     gene            complement(8647..9186)
                     /gene="csi"
                     /locus_tag="I6L15_25970"
     CDS             complement(8647..9186)
                     /gene="csi"
                     /locus_tag="I6L15_25970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="QXA90883.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMAIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMTNNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            9216..9371
                     /gene="csl"
                     /locus_tag="I6L15_25975"
     CDS             9216..9371
                     /gene="csl"
                     /locus_tag="I6L15_25975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="QXA90884.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GGSGSL"
                     /name="csl"
     gene            complement(10369..10560)
                     /locus_tag="I6L15_25980"
     CDS             complement(10369..10560)
                     /locus_tag="I6L15_25980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QXA90885.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            10625..10948
                     /locus_tag="I6L15_25985"
     CDS             10625..10948
                     /locus_tag="I6L15_25985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QXA90896.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            10890..11645
                     /locus_tag="I6L15_25990"
                     /pseudo=""
     CDS             10890..11645
                     /locus_tag="I6L15_25990"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            11626..12144
                     /locus_tag="I6L15_25995"
     CDS             11626..12144
                     /locus_tag="I6L15_25995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QXA90886.1"
                     /translation="MSNLLQTRAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            12151..12384
                     /locus_tag="I6L15_26000"
     CDS             12151..12384
                     /locus_tag="I6L15_26000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QXA90887.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          3061..3147
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.727"
                     /coverage="100.0"
                     /evalue="5.25e-35"
     Typing          10197..10284
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          complement(2493..3037)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.526"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(9629..10173)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.536"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3806..5400
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.248"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          10939..12488
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.183"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatatactgc tttgatatct cacacatcac aaatcttctg cctaaatcaa ttgctgcttt
       61 tgctgttgtt cccgatccag caaaaggatc taaaataaca tcatttttga aagaatagta
      121 ttttataact ctttctgcca gaccgtatgg aaatgtagca ggatgttttc tgttacgaga
      181 tggatttatt ttccataggt ttgtcttttc gtaatcgtcg ccgatttttg agtcaaaaac
      241 atcctctttg ctatgatggt ttcttatgtt ccaatctatt aatttatcag ttttttttct
      301 gtaaacaaga atgtactcag ttactggtac tggtttatat tgcaacggat tgcggtcagc
      361 tgcgaatcga cggcctctac ctaaagccca gccagctcct tctggtttaa cccaatgtat
      421 atcatctatg aattcatagc cttcttctat aaataacctg tgcaagtcaa aaggaacagc
      481 tattcttttt gatgcttcat tccgtgacgc ccttcttatt aaaacgggcg agacattgat
      541 gacgaaaaaa cgaccttcgc ttaaaactct atggcactct tttattactg aacgcaatag
      601 agagaggtat tcatcatatg tatgatattc ggaatactct ggtttggcat tgtaatatgg
      661 cggtgatgtg aatactaagt tcactgattc atcaggtaat tttttcaagg tctccgcgca
      721 gttgccttgt agtaacatgt tatttaatgg tgactttgat atgatttttt tttctttttt
      781 tacctttttt ggggtatgga caacctccat tgctttctta ataagaaatg gagacttcga
      841 acttttctta taatcaatag ttgacgtttt tgaatttata actatttcac caatcctgtg
      901 gcctgtcgct actaatggta cacgccatac atggtaccga tcatcgactt caggtaaccc
      961 aaaggaaaat ttatcagcta agtctaattt ctctaggtgc aactttgcta tttttttcgc
     1021 ctgagtcgta gaggttacca aataacctct gtttaagtaa ttgtttttaa ctgtcatttt
     1081 tcctaactat cgatctgtta ccatacaacc tgctatctta gcatctcatt tttactatgt
     1141 ccatggtgag gatatgaatt tcaaggataa aaattgtttc cctaacgaac tcatagcgtt
     1201 ggcgaaaatt tcaaaaaatg atgttttaga taagttcgga acggatgttt ttaaaaaggt
     1261 tgtttatgat gttttaacag gtaaaaatgt tcgcgaattc actgaaatac taactcgtac
     1321 tagattgtta gaaagcaatc tctctttttt tgactttttt gtggataaaa tgaaagaagg
     1381 gataacgcca aagcagcttt atctctatgc aaaaaatgca ttatcgaaca agtcttatgt
     1441 taagtctaat caacctgttc tcgagtggat ggttatgatg acaaataaac agacccaaaa
     1501 tgttttaaga gatgagcatg gggatggttt tgatagactt gctttaagga cgcaagaaga
     1561 aatacttaaa ataaaaaacg ggtatgaaga taaaattgga gagatatcta ttggtgggca
     1621 aaaggtgtct ttagaagatt tttgctatat tattttatct cttggttcgc aaactttaac
     1681 tattagggga tctgagaaat ctcttcatgg taaatatttt gaaaagctaa tactcggttc
     1741 tttatttaca ataatgggtt ttgaatataa agaaaaaatt gaagaagggt taaatgctaa
     1801 atgttttact ctttcaacaa gagctgatga cagggagtct gatgctactc ttgtttttaa
     1861 tgggaaggcg attagggttg acattggttt tattggtagg ggtaacacag aaataagttt
     1921 ggataaagta tctagattta gacgaatgga tgatattggc ggagtgatgc ataatataag
     1981 cacaatggtt attgttgacg ttattggtga tagaagtaga atagttaata tggctgaaga
     2041 gattgatggt aaagttgttg cgatgagtga tccgtattgg gttgcaaagg tctcttccta
     2101 tattagttcg aaactgaatg tagatgatct tttagaggat aaacctcaac ttaaagacat
     2161 acagtctttt atatctgatg cattagagaa tgtagatctg gaaaaataca ttaaattata
     2221 agagattgta atccttgact gcgtgcttat taaaagcata atctctacat aaagaaaaac
     2281 caccctggca ggtggttttt caaaggttag ctaatcctgg cagattatct aaccaggtaa
     2341 tctggcttaa gcagagcgca gaccaccaaa acttgttctt ctagtgtagc cagaatcagg
     2401 ctgatacttc aagaactctg ttaccaactt ataaatctcg ctctgctaac ccggttacca
     2461 atggctgctg ccagtggcgt ttttacgtgt ctttccgggt tggactcaag acgatagtta
     2521 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2581 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
     2641 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2701 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2761 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2821 gccagctacg cggctttttt tgccctttgc ctttttgctc acatgttctt ttctgcctta
     2881 tcccctgatt ctgtggataa ccgtatcgcc gtttttgagc cagcagactc cgctcgccgc
     2941 agtcgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaaaagcg cctggacgag
     3001 cattttctcc ttacgcatct gtgcggcatt tcacacccgg catggtgcac ttttcataca
     3061 atgcgccctg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
     3121 tcatggctgc gccccgacac ccgctaaaac ccgctgacgc gccctgacgg gcttgtctgc
     3181 tcccggcatc cgctcacagg ccagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     3241 tttcaccgtc atccccgaag tgtgcgaggc agctgcggta aagctcgtca gcgtggtcgt
     3301 gaagcgcttc gcagatgtca gcctggtcgt cgacgtccag ttcattcagt ttttccagca
     3361 gggtcagcgt ctggcttctg atgaagcggg ccatgttaag ggcggttttt tcctgtttag
     3421 tcacagttct gtttctccat gtgtggggag tctgtcggag gtttttaatg ataccggtga
     3481 aacgcgaaag gatgctcacg atacgggtca cggatgagga acatgcgcgg ctgctggcac
     3541 gttgtgaggg aaaacaactt gcgtcatgga tgcgtaaggt ctgcctgggg gcaccgccat
     3601 cgaaaacgtc aggtcttccg acactggcac cgccgctgtt gcgccagttc gcttcggtcg
     3661 gcaataacct gaatcagata gccaggaaga taaacagcgg acagtggtcg gggcatgacc
     3721 gggttcatgt ggtggctgca ctgatggcca tcggacgtga actgagtgag cttagggatg
     3781 aggtccgtaa acagggagaa cgggatgata gttaagtttc atgccagggg aaaaggcggt
     3841 ggcagtggtc cggttgatta cctgctgggc cgggagcgta accgggaagg ggcgagagtt
     3901 ctgcggggtg ctcccgaaga ggtacgggaa ctcatcgatg ccacgccgtt tgcgaaaaaa
     3961 tacacctcgg gggttctgtc ttttgcagag cagaccctgc caccgggaga gcgtgaaagg
     4021 gtgatggaga gctttgagcg ggtactgatg cccggtcttg aaaaaaatca gtacagcatc
     4081 ctgtgggtgg agcaccagga caaggggcgt cttgagctga attttgtcat cccgaatatg
     4141 gagctggcga gcggaaaacg gctccagccc tattacgacc gggcggaccg tccgcgaatc
     4201 aatgcctggc agacactggt gaatcaccat tacgggctgc acgacccgaa cgcaccggag
     4261 aacaggcgga cacttgtcac cccgaataat ctgccgaaag cgaaacagga ggccgcagaa
     4321 gcgattacga ggggactgga ggctctttac catgccggag cgctaaaaac gcgtcaggat
     4381 gtcactgagg cgcttacagc ggcaggtttt gaggttgtga ggacaacccg gagcagtatc
     4441 agtatcgccg acccggaggg cgggcgtaat ctcaggctga aaggggcgct gtatgagcaa
     4501 tcttttacaa atggggacag acttcgagaa gaaactgaaa gagcgtgccg tctctacaga
     4561 gaacatgctg aacagcgagt tcaggaagct cgaagcgtct gtagacaggg aactgaactt
     4621 aaacgggcag aaaatcagag acgctatcag cgcacacaca gcagcggtga aagaacaact
     4681 ggagacgctg aacagtgccg tgaacgcgca gttcagcgcc acagagaacg aactggcccg
     4741 gcggcaggaa gagctgctct ggaagctgat caaagggcgg atactgtacc cgtcgctgac
     4801 ggcgctttgc gtcacgggcg ggatttttct ggcgtcatgg gggctgattc agtggcagga
     4861 aagccggatt gcggcgaaca tcctggctat cagggaccag gaagagacgc tggcgaaact
     4921 ccgggaaaaa acatggggtg tgacgtatca cgagggcagg aacgggaaat ttctggtgct
     4981 gccgtccggg gtgaaggggg aaaacaactg gacggtggag aagaagaacg cagtaagact
     5041 ggtcagggag taagtgagta tgacagagct ggaaatgcac ttactgaacg ccttagagca
     5101 attgcagcag gactatacgc agcggctgaa agactgggag agcgccttca cggatttgca
     5161 gcatatgttc gagctgacac gacaggagaa cgggcaactg agggaacagt gcgacgactt
     5221 gagtcagcag gtgcagagat tagccgggca ggtggaccgc ttgagtcggt tatttatcac
     5281 gaacagcaga taagggatga acaggaaagg caggcacgcc tggaacggga gcgtgcgcgg
     5341 tcctggcatg aagagcagga acgggaagca caggtatggc gtggcccgtc aatggggatg
     5401 tgacggtgag ttgttccggg aggttgccag atggtaccgg aacgcgttaa aaacgcatcc
     5461 gggcgcgatt ctggcgtgtc gtttttgttg ctgtgttttg cctctttgcc agatcgcgct
     5521 cattcctcag cctcatcccg cgatgtctgc gtatgtcatc aactgaaaag atggaaaaaa
     5581 aacagtgtca tgaggtttac gcaggcaaaa ccagcgttat tcacatggct gaatagctat
     5641 gcatacggtt aaaatttatc aggtgcgatc gcggcagctt ttcgggtggt ttgttgccat
     5701 ttttacctgt cagcagccgt gatcgcgctg aacgcatttc agcggtgcgt acaattaagg
     5761 gattatggta aatcccttac tgtctgccct cgtatccatc gagataaacc gcagaaatgg
     5821 tgtctgccat tagtttcatt tttttcagcg aaggcatatc gctgtttgtg tattttctgc
     5881 cagcaaaggt tgttgtgctc tggagtgagt gaaaaaattt ttctgagtgg aattcttttg
     5941 cacgttgctg tgccgtttct gctgatactc cggctgcttt gtctttcatc cccatatccc
     6001 ggaactgctg aaagaacggg agcatcatac gtatggtgtc tgctttctgg gtatagaagt
     6061 taggatcaaa tccatcatct gaagcataaa cataatgtcg ggtatgtcgt tccaggctgg
     6121 tctttggggt gcatcctgta agagctattg ttgctgttat cagaggtatc agaagcagcc
     6181 tggtagcggc agatctcata aacatctcct tgcgttttta atcttgatac tcagggagtt
     6241 atatctccga agagtatctc ttatcatacg tcggtttact cccaatgtga tttgatggtc
     6301 aatgagtcag gaataatccc gtgaaaaaat gtgtgccagt cctgcttttt agtgttcttt
     6361 ctgcttcggt tgccagtcat gcggacagca atgtttttca acaaaaggcc gagaagacaa
     6421 aaaacggcat tgttgcggat gttaatgcca gacagaaaat gttcagtaac agactgaaga
     6481 tacaacttca gatcgatgag ttgagagcag agctggctgg caccagagat gcccaaaaaa
     6541 gagaggattt gcgtaaaaaa ctggagtctc tggagcggca aaagaaatcc ttacgttgat
     6601 gggggcgtat tttccccggt ttcttccata acctttttac cgttcagata tttacgggcg
     6661 aggcgaatgc gtcttgcctg gtataccgcc gagtaagcct gggtgggatt cacaccatta
     6721 ctgatttcac tgaccacatg acgctgatca gccagtgtca gcgtgtaaac ctgtttctgg
     6781 atgaggtcac gggctttgtc atgctcatat ccggcagcaa caagggtgtc gagaagtcgt
     6841 ttgtctgttt tgttcagttt atggcggggt gatttgctca catcgttctc cgtttttcat
     6901 gatccaacag ttttatatct catcctgatt tgctccggtg ttctgtttcg acgttctcaa
     6961 tgcctgcctc cccatagcgt gatccttata gcaaaaatga aaatccatgc tcttgacatg
     7021 gacaatagtg agtagtaggt tttgctgtac ataaaaccag tggttatatg tacagtgttc
     7081 tgtttttaat ttattgtttt aattatcaaa gaggaattta tggctaaaga actaagtgga
     7141 tatggcccaa ctgctggtga gtctatgggg ggaacagggg cgaatctgaa tcagcaaggt
     7201 ggaaataata atagtaatag tggggttcac tggggtggtg gttcaggctc tggtaatgga
     7261 ggtcgtgaac atggaagtca gacggggtgg ggatggagta aaaccaataa tccggatgtg
     7321 ccgccttatg ttgatgataa tgggcaggtg cgcattacca tcacaaatgg tctggtaaaa
     7381 acgcctgtat atggagttcc tggggctggc ggaaatagtg atgttcaggg gggctatatc
     7441 cctgaaaatc ctaatgatga agttgctcgt aagtgggata aaaacaactt accgcgagag
     7501 attgatgtga gtatcgatgg ttttaaatat cgtgtaacgc ttaatgataa tggtagagct
     7561 atcggtattc ttcgaacagg tgtccgtcct tacgtaggga gtgaaaaagc taaggcgggt
     7621 attatggaaa aaatcaatca taaaactcct gaggagatat atgaggcgtt aggttttaat
     7681 aaagatgaat ctcagcggca ggaaaaagca aaacaacagg ctgaagatgc gtgggatcgt
     7741 ttgcctccta atgtgagaaa atttgatgtt gatgttgaac agttccacta tttagtggtg
     7801 cttgatgatt atggtaatgt tttatctgta actagaacag ctgttcgtcc ttatgtaggg
     7861 agtgaaaaag ctaaggctgg tattatggat aaagtcgacc ataagacccc ggaagagata
     7921 tatgaggcat taggatttaa taatgaagag cctcaacgac aggatcaagc taaaaaagct
     7981 gcttatgatg tgttttattc tttcagtatg aaccgtgacc ggattcagag tgatgtactt
     8041 aataaagccg ctgaagttat ctctgatata ggtaataagg tcggtgatta tcttggagat
     8101 gcttataagt cgttagcaag agaaattgct gatgatgtca aaaatttcca ggggaaaact
     8161 atccgtagct acgatgatgc aatggcgtca ttaaataaag ttttgtctaa tccggggttt
     8221 aaatttaaca gggccgacag tgatgcactg gcgaacgtct ggcgaagtat tgatgctcag
     8281 gatatggcaa ataaactggg taatatcagc aaggctttca aatttgccga tgtggtgatg
     8341 aaggttgaga aggtccggga gaaaagcatc gagggctatg aaaccgggaa ctggggacct
     8401 ctgatgctgg aggtggagtc ctgggtgctc agtggcatcg cttctgctgt ggccctgggt
     8461 gttttttcag ccacattagg ggcatatgcc ttatctcttg gggctcctgc tattgctgtc
     8521 ggtatcgtag gtattctact cgcagcagtt gtaggtgcat taattgatga caagtttgca
     8581 gatgcactaa ataaagaaat cattaaaccc gctcattaat aaataccggg taaaaggaag
     8641 gcagggttac tgcctttctt ttaataattt aaacgtggta actggtgtga atagcgtgaa
     8701 atagcttatg aaaaaaatga ttaagtacag gccaatataa aatagcaata gtgttgcatc
     8761 attgttggtc attagcctgg ctggccttcc tgctgtcgtt agctcaaggt ttcggaaaca
     8821 gagtaaatac aaaaaaacag cacagacaaa tggacttaat aagcaacttc taagtacagc
     8881 atttcggttt gtttttttta ttagctttct cgttttaaat gtcaggataa ataatatcaa
     8941 agcaagggaa ggcgctgttt tacaataaat atccataacc tttgtcatta atgggttgta
     9001 ggcggaggtt attgatggga tgttttcagt actggtagct attgtgtgca agaacagatt
     9061 gtctggattt gtatagtaaa cggctagtat acagagtaaa ggtataactc cgattgccat
     9121 aaataaatac aataagttat tggcttttct attgtctata tctattgaat gttcatcaat
     9181 catcattgac ctataggatt ataataaagg tatatatgaa aaaaattatt atttgtgcca
     9241 tttcactagg aataatgctg cttgcagcat gccaagtcaa caatgtcaga gatactggag
     9301 gtggggctgt ttcaccatct tccactgtta ccggagtgag tatgggaagt ggtggtagtg
     9361 gtagcctgta aaggatcttc ttgagatcct ttacagatcg tcgtagtctc ttgccctgta
     9421 aacgaaaaaa ccgcctgggg aggcggttta gtcgaaggtt aagtcagttg gggactgctt
     9481 aaccgggtag ctggcttaag tggagcacag ataccaaatg ctgtcctttc agtgtagccg
     9541 ctataaggct tcaacttcaa gagttctgcg tatctaaatc cacttttcag ttaccaatgg
     9601 ctgctgccag tggcgctttg tcgtgtctta ccggggtgga ctcgagacga tagttaccgg
     9661 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9721 cgacctacac cgaactgaga taccaacagc gtgagctatg agaaagcgcc acgcttcccg
     9781 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9841 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9901 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9961 gctacgcggc tttttttgcc ctttgccttt ttgctcacat gttcttttct gccttatccc
    10021 ctgattctgt ggataaccgt atcgccgttt ttgagccagc agactccgct cgccgcagtc
    10081 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga aaagcgcctg gacgtgcatt
    10141 ttctccttac gcatctgtgc ggcatttcac acccggcatg gtgcactttt catacaatgc
    10201 gccctgatgc cacatggtta agccagtata cactccgcta tcgctacgtg actgggtcag
    10261 ggctgcgccc cgacacccgc taaaacccgc tgacgcgtcc tgacgggctt gtcggctccc
    10321 ggcatccgct cacagacaag ctgtgaccgt ttccgggagc tgcatgtgtc agaggttttc
    10381 accgtcatcc ccgaaacgtg cgaggcagct gcggtaaagc tcatcggcgt ggtcgtgaag
    10441 cgattcacag atatctgcct gttcatctgc gtccagttcg ttaagtttct ccagcagcgt
    10501 taatgtctgg cctctgatga aacgggccat gttaagggcg gttttttcct gtttagtcac
    10561 tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgcga
    10621 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgctgtga
    10681 gggtaaacaa ctggcggtat ggatgcggcg ggtctgcctg ggtgagccgg ttgcccgttc
    10741 cgggaagctg ccgacactgg caccgccgtt actgcgtcag ctggccgcca tcgggaataa
    10801 cctgaatcag acagcgagga aggtgaacag cggacagtgg tcttccggtg accgggttca
    10861 ggtagtggcc gcactgatgg ccatcgggga tgagctgcgc cggctgcgtc tggccgtcag
    10921 ggaacagggg acgcgggatg atagttaaat ttcatgccag gggaaaaggt ggtggcagtg
    10981 gtccggttga ttacctgctg ggaagggagc gtaaccggga aggggcaacg gtgcttcagg
    11041 gtaatccgga agaagtccgg gaactcatcg atgccacgcc atttgcgaag aaatacacgt
    11101 caggtgttct gtcgttcgcg gagaaggagc tgccgccggg aggacgtgaa aaagtgatgg
    11161 cgagctttga gcgtgtactg atgcccggtc tcgaaaaaaa tcagtacagc atcctgtggg
    11221 tggagcacca ggacaaggga cggcttgagc tgaattttgt cattccgaac atggagctac
    11281 agagcggaaa acgcctccag ccgtactacg accgggcaga caggccgaga attgatgcct
    11341 ggcagacgct ggttaatcac cattacgggc tgcatgaccc caacgccccg gagaaccgcc
    11401 ggatactgac tctccctgat aacctgccga aaacgaaaca ggcgcttgca gagagcgtca
    11461 cacgcgggat agacgctctt tatcatgtcg gggagataaa aggccgtcag gatgtgattc
    11521 aggcgctcac ggaggcaggg attgaagtgg tcagggtgac gcgaagcagt atcagcattg
    11581 cagacccgaa cggcgggagg aatatcaggc tgaaaggagc attttatgag caatctttta
    11641 cagacgcgcg cggagttcga gaaaaagctg aaagagagag ccgaatctac agagaaaatg
    11701 ctgaacaacg agttcaggag gctcggcgag tctgtcagcg aggctgtgac ctcaaacgag
    11761 acgaaaatca gagacgctat agccctgttc acagcctcga cagagaaatc gctggaaaaa
    11821 caccgggaag gggtgaaaga ggcgatgatg cagcacagga aggacgtgtt aaagctggca
    11881 gggaatacgg gcatgatgtt actggggata gtctttctcc tgtttacagc gagtggcggg
    11941 acgctctggt atcttggagg gaggatacag gcgaacctgg aagaaatcag gaagcaggaa
    12001 gagacattgc agaaactgaa cgcgaagaca tggggcgtgg agtttgtgca ggacgggaac
    12061 aggaaattcc ttgtccttcc gtacgggaaa tcagcggagg tgattccctt tcaggggaaa
    12121 gagtgggtac atctgaagga gtgacacaga gtgacagagc tggaaacaca tttgctgaac
    12181 gccttagagc agctgcaaca ggactatatg cagcggctga gcgaatggga gagcgccttc
    12241 gtggaattgc agaagatgtt ttcgcttacg caacgggaca acgcgatgct gaacgagcgg
    12301 gtcatgcagt tgagtcagca ggtgcagcac ttgagcgagc agacagaacg cttgagccag
    12361 ttatacagcg agaactggag ataagagagg aacggctgat acaggagcgc gaacatgtgt
    12421 tatccctgga acgggagcgt cagccggaaa tacaggaacg cacgctggat ggcccttcgc
    12481 tgggatggtg aaaccatgaa aaatggcagg ttaagtggat taagacaggc taatgtggtc
    12541 tgtaccatct ggttgcatag gtattcatgc ggttaaaatt tatcaggcgc gattgcggca
    12601 gtttttcggg tggtttgttg ccatttttac ctgtctgctg ctgtgatcgc gctgaacgcg
    12661 ttttggcggt gcgtacaatt aagggattat ggtaaattcg acaatgtggc tgatgttgac
    12721 gcgtaaataa gccgccttaa ggcggctttt accacagtaa agtttgatta atttatgatt
    12781 tttaaatcac cacctagcat ggttccttct atgatcagat c
//
