LOCUS       CP077267                3374 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1378 plasmid unnamed1, complete
            sequence.
ACCESSION   CP077267
VERSION     CP077267.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357520
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1155.05x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:16:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,864
            CDSs (total)                      :: 4,742
            Genes (coding)                    :: 4,605
            CDSs (with protein)               :: 4,605
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 58 of 137
            Pseudo Genes (incomplete)         :: 68 of 137
            Pseudo Genes (internal stop)      :: 38 of 137
            Pseudo Genes (multiple problems)  :: 23 of 137
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1378"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1378"
                     /db_xref="taxon:562"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            complement(712..905)
                     /locus_tag="I6L08_23985"
                     /pseudo=""
     CDS             complement(712..905)
                     /locus_tag="I6L08_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            979..1293
                     /locus_tag="I6L08_23990"
     CDS             979..1293
                     /locus_tag="I6L08_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QXA71824.1"
                     /translation="MRVTDDEHARLLERCEGKQLAVWMRRVCLGESVARSGKLPTLAPP
                     LLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQMVREQRVQDDS
                     "
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1283..1993
                     /locus_tag="I6L08_23995"
                     /pseudo=""
     CDS             1283..1993
                     /locus_tag="I6L08_23995"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1971..2489
                     /locus_tag="I6L08_24000"
     CDS             1971..2489
                     /locus_tag="I6L08_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QXA71822.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            2496..2729
                     /locus_tag="I6L08_24005"
     CDS             2496..2729
                     /locus_tag="I6L08_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QXA71823.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          538..623
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.552"
                     /coverage="100.0"
                     /evalue="3.06e-32"
     Typing          complement(2..520)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.084"
                     /coverage="94.0"
                     /evalue="0.0"
     Typing          1284..2833
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.979"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
       61 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      121 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      181 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      241 gacttgagcg tcggtttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      301 gcagcgtggc cttttccggg ttcttgtttt ttgctcacat gtttcttttg gcgttatccc
      361 ctgattctgt ggataaccgc atctaccgtc tttgagtgag ctgataccgc tcgccgcagc
      421 cgaacggccg agcgtagcga gtcagtgagc gaggaagcgg aagagcgcct gaatgcggca
      481 ttttctcctt acgcatctgt gcggtatttc acatcgacat ggtgcgcttt ccatacaatt
      541 cgtactgatg ccagcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
      601 atggcttcgc cccgacaccc gctaaaaccc gctgacgcgc cctgacgggg cttgtctgct
      661 cccggcatcc gctcacagac aagctgttac cgtctccggg agctgtatgt gtcagaggtt
      721 ttcaccgtca tccccgaagc gtgcgaggca gctgcggtaa agctcagtcg gcgtggtcgt
      781 gaagtgattc acaaatatcg gcctgttcat ctgcgtccag ttcgttgagc tttctccagc
      841 agcgttaatg tctggcttct gataaaacgg gccatgttaa gggcggtttt ttcctgttta
      901 gtcactgatg cctccgtgta agggggattt ctgtttatgg ggtaatgata ccgatgaaac
      961 gcgagaggat gctcaccgat acgggttact gatgatgaac atgcccggtt actggaacgc
     1021 tgtgagggta aacaactggc ggtatggatg cggcgggtct gcctggggga gtcggttgcc
     1081 cgttccggga agctgccgac actggcaccg ccgttactgc gtcagctggc cgccatcggg
     1141 aataacctga atcagacggc gaggaaggtg aacagcgggc agtggtcttc cggtgaccgg
     1201 gttcaggtgg tggccgcact gatggccatc gagagagagt tgcgcagctt acggcagatg
     1261 gtcagggaac agagagtgca ggatgatagt taagtttcat gccaggggaa aaggtggtgg
     1321 cagtggtccg gttgattacc tgctgggaag ggagcgaaac cgggaagggg caacggtgct
     1381 tcagggtaat ccggaagaag tccgggaact catcgatgcc acgccatttg cgaagaaata
     1441 cacgtcaggt gttctgtcgt tcgcggagaa ggagctgccg ccgggaggac gtgaaaaggt
     1501 gatggcgagc tttgagcgtg tactgatgcc cggtctcgaa aaaaatcagt acagcatcct
     1561 gtgggtggag caccaggaca agggacggct tgagcttaat tttgtcattc cgaacatgga
     1621 gctacagagc ggaaaacgcc tccagccgta ctacgaccgg gcagacaggc cgagaattga
     1681 tgcctggcag acgctggtga atcaccatta cgggctgcat gaccccaacg ccccggagaa
     1741 ccgccggata ctgactctcc ctgataacct gccggaaacg aaacaggcgc ttgcagagag
     1801 cgtcacacgc gggatagacg ctctttatca tgtcggggag ataaaaggcc gtcaggatgt
     1861 gattcaggcg ctcacagagg cagggcttga agtggtcagg gtgatgcgaa gcagtatcag
     1921 cattgcagac ccgaacggtg ggaagaatat caggctgaaa ggagcatttt atgagcaatc
     1981 ttttacagac gggcgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga
     2041 caatgctgaa cgacgagttc agcaagctcg aaaaatctgt aagcaaggct gtgacatcaa
     2101 acgagacgaa aatcaaagac gctatagccc tgttcacagc ttcgacagag gaatcactga
     2161 aaaaacaccg ggaaggggtg aaagaggcga tgatgcagca caggaaggac gtgttaaagc
     2221 tggcagggaa tacgggcatg atgttactgg ggatggtcct tttcctgttt accgtgagtg
     2281 gcgggacgct ctggtatctt ggcgggatga tacaggcgaa cctggaagaa atcaggatac
     2341 aggaagaaac attgcagaaa ctgaacgcga agacatgggg cgtggagttt gtgcaggacg
     2401 ggagaaggaa atttctggtt atccctcagg ggaaatcggc aacggtgatt ccctatcagg
     2461 ggaaggactg ggtacaactg acggagtgac acagagtgac ggagctggaa agacatttgc
     2521 tgaacgcctt agagcaactg caacaggact atatgcagcg gctgaacgaa tgggagagcg
     2581 ccttcgtgga attgcagaaa atgttttcgc ttacgcaacg ggacaacgcg atgctgaacg
     2641 agcgggtcat gcagttgagt cagcaggtgc agcacttgag cgagcagaca gaacgcttga
     2701 gccagttata cagcgagaac aggagataag ggatgaacgg ctgatacagg aacgcgaaca
     2761 tgagttatcc ctggagcgtg agcgccagct ggaaatacag gaacggacac aggatggccc
     2821 ttcgctggga tggtaatcag agacaggcta aaatgttgtc agcgcgtttc cggtgcattt
     2881 tttgggataa tggttggttc gggggcaccg ggaacgcgct tcagtcagac cttgctgaaa
     2941 aaacaggaac gcgttaaaac gcatacgggc gcgattctgg tgtatctttt gttgctgtgt
     3001 tttgcctcta ttgccagatc gcgctcattc ccctgaactt ctgttttccc gctccggttc
     3061 ccgtcttctt cgcttccgaa agcatgaaaa aaatccgttt cagcagtctc tggctggaaa
     3121 ttcctgcgtg attcacatgg ctgcatagct atgcatgtgg ttaaaattta ccgggtgcga
     3181 tcgcggcagt ttttcgggtg gtttgttgcc atttttacct gtctgctgcc gtgatcgcac
     3241 cgaacgcgtt tcagtggtgc gtacaattga aatgttatgg taaatagctt ttataacatc
     3301 ctgattaact taaatcgttt tcaaaatgaa tactattgtc tgtagagcaa tggtattcac
     3361 atggtggatt attt
//
