LOCUS       CP077257                3845 bp    DNA     linear   BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1381 plasmid unnamed7.
ACCESSION   CP077257
VERSION     CP077257.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357523
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 886.95x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:20:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,078
            CDSs (total)                      :: 4,960
            Genes (coding)                    :: 4,811
            CDSs (with protein)               :: 4,811
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 68 of 149
            Pseudo Genes (incomplete)         :: 88 of 149
            Pseudo Genes (internal stop)      :: 34 of 149
            Pseudo Genes (multiple problems)  :: 38 of 149
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1381"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1381"
                     /db_xref="taxon:562"
                     /plasmid="unnamed7"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            <1..488
                     /locus_tag="I6L11_25375"
     CDS             <1..488
                     /locus_tag="I6L11_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="QXA42728.1"
                     /translation="EWREEAYTLDELADYLDLSASERRSVDKHYGLGRNCHLFEITRKW
                     AYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSLAECRAIGKSIAKYTHRKFSPEGF
                     SAVQAARGRKGGLAKGEAYEDKRFMALCMLENGYSQKAIAAMLDVHRNTIRNWAMHK"
                     /name="primase C-terminal domain-containing protein"
     gene            complement(694..969)
                     /locus_tag="I6L11_25380"
     CDS             complement(694..969)
                     /locus_tag="I6L11_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QXA42729.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPRPPAKPQNG"
                     /name="mobilization protein"
     gene            1148..3367
                     /locus_tag="I6L11_25385"
     CDS             1148..3367
                     /locus_tag="I6L11_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QXA42730.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAREA
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFVAMNGKANDIKREGNALVKERDILEKEMTGLRKARPVASI
                     LSELPLMTWAEPEYRKRQLRFWKLEKQIESLLRTYRAVKERDIPVRRQAFETQWNTWIS
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEYDARLKHHDNHRLSRETALAGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPAA
                     SPLPSAHRVDVTAYAAPEQLMPVVFSTERNSRTLNLLLCKGPEEMLVGFVRLEDELRPV
                     LALPSPDYSHLMVSIITENGIHLAGYGEAINRDADTPYPPEPKLMQFRLKGCQDTLFAA
                     INKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHYRGMSM"
                     /name="MobA/MobL family protein"
     gene            complement(3625..>3845)
                     /locus_tag="I6L11_25390"
     CDS             complement(3625..>3845)
                     /locus_tag="I6L11_25390"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA42731.1"
                     /translation="FFEVLKRGAHVLRFLPGYRPTSPLYLRESLGGSTRISGAFVFQSL
                     TLHPTANPVDPSRKTDPFTITPVVRTP"
                     /name="hypothetical protein"
     Typing          complement(974..1147)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="98.0"
                     /evalue="1.23e-74"
     Typing          complement(1148..1506)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..488
                     /name="rep_cluster_2361"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1149..3364
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.099"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggagtggcg cgaggaagcc tataccctcg atgaactggc tgattatctc gatttgagcg
       61 cctcagagcg tcgtagcgtc gataaacatt acgggctggg gcgaaactgc cacctgttcg
      121 aaataacgcg taaatgggct tacagagcga ttcgtcaggg atggccagca ttctcacagt
      181 ggcttgatgc cgtgatccag cgtgtcgaaa tgtacaacgc atcgcttccc gttcctcttt
      241 cactggctga atgccgggct attggcaaga gcattgcgaa atacacgcac agaaaattct
      301 caccggaagg cttttccgct gtacaggccg ctcgaggccg gaaaggtggc cttgctaaag
      361 gcgaagccta cgaggacaag cgtttcatgg cgctatgtat gctggagaat ggatattctc
      421 agaaagctat tgcggcgatg ctggatgtac atcgaaatac gatccgtaat tgggcaatgc
      481 acaaatagcc ttatcagata acagcgcctt ttcggcgctt ttttgagctg ttggcgttcc
      541 tgcggttttt ccggttccgc ccagcaatgg cgctggtttc ctcgtgattg cggtggtagc
      601 gctcgccgca gcctcatgac cgagcgtagc gagcgaatgt gcgaggaagc gcaaaggcgt
      661 ccggtggtgc atgtggcact tacgcgccgg ggcttatccg ttctgcggtt tcgcaggtgg
      721 tcttggtagc ttctccagct cgttaatcag tggttgtagc cggttcacat cggcctgtct
      781 tgtgacttcc tttcgcagga actggagcag gaacgcacgc agttgcgcct cttccggcct
      841 ccgtaccctc gccagcatgg ctgctcccac aatgactttt tgcgcagtgt ccaggctccg
      901 gctcttcgcc ttcaggcgct gcaatctggc ctcagcttca gcaatcttct gttcgagtgt
      961 tctgctcatt tcgtgactcc ttgcgcggtg aaaaatcgca ttttagctcg tcactggtag
     1021 tttaaaaact gagctggcat aatgcacggc atatcacgaa gtgcgcactt atacaatctt
     1081 cacttcgttt cgattgtgtg cgccctgcgg ggctgaaaga aaacggcaaa aaggcattac
     1141 ggcagaaatg gcgatttatc atctcagcat gaaaatcatt tcgcgaaaaa acggctacag
     1201 tgccgttgct tctgctgcct accgttccgg ctctgtcata cccgatgacc gtaccggatt
     1261 aatccacgat tacacccgta aacgcggcgt tgatgatgcg gtcattctca cccctgcgaa
     1321 tgcaccgtcc tggtgtggtg accgttccgt tctctggaat gcggtcgaga aagccgaaca
     1381 gcgccggaac tcccagctgg caagggagat tgaactcgcc attccccgtg agatttcccg
     1441 cgaggccgca cgggaggccg ttctcgcttt tgtccgggaa aactttgtca gtcggggcat
     1501 gattgccgat gtggcgttcc atcatatgga ccggaccaac ccccatgcac acatcatgct
     1561 gaccacgaga gctgtcgggg aaacgggatt cgcaggaaag gtcagggact ggaacgaccg
     1621 ggcactcgcc gagacgtggc gcgcatcatg ggctgaccat gcgaacagag cgcttgcgaa
     1681 cgccggctac caggaagaga tagaccatcg ttcatacgag cgtcagggac tggagaaagc
     1741 gccgggcctt cacctcggaa aggctgcctg tgcgatggaa aaacggggca tggaaacaga
     1801 acgcggtgag cagaaccgcc tgattaacag ccttaacctg gaaatacagg tttcccgcac
     1861 aaggctggca gtcatccagg tacaggagaa cgtgcggaaa cgggaactca gcgatgccgc
     1921 acgtcgtgca gcggaagccc ttaatctgac cattcccgcc gcgaatgcct cagcggatac
     1981 cctgcgggaa ttcattgcca cactgccgca ggaatgcggg aacgcatggg agatgacacc
     2041 ggagttcgtg gcgatgaacg ggaaggcaaa cgacatcaaa cgtgagggga atgcgctggt
     2101 gaaagagcgg gatattctcg aaaaagagat gaccggactc agaaaagccc gtccggtagc
     2161 gtccatcctg tcagagcttc ccctgatgac atgggctgaa ccggaatacc gcaaaagaca
     2221 actccgcttc tggaaactcg agaaacagat tgaatctctt ctccgcacct acagggccgt
     2281 gaaagaacgg gacattcccg tccgtcgtca ggcctttgaa acgcagtgga atacgtggat
     2341 ttcgccggga atggcagagc tgaaagaaaa actgtcagca cgggaagcgg agcggcgcag
     2401 ggaggaagcc gaagcggaag cgcgccggaa ggaacaggaa tatgacgcac ggctgaaaca
     2461 ccatgataac catcgcctga gccgagaaac ggcattagcc ggggttatta cggagctggg
     2521 gcgtgccaga gagccgggaa cgggcaggat aacccgctac atgatgctga gtaacagagc
     2581 cggagaattc acggtatggg gtgatgagct ggcgcattac ccccagagtg ttcatgaccc
     2641 ggtgaatgtt tacctgtcgc caggcggggc tgtgatggtc tcggatatac gtgagggaat
     2701 gccagaatct catgagacga tggcgcggcc tgagcgtgtg agaatgtatt ccggtgcgac
     2761 ggtccggcat gtactggaac agatgcgcca ggggtggccc tcttacggtt ttccggcgct
     2821 gccgcatcac tggccggata atttttattt cagcgacgac cgcaggcccg cagcctctcc
     2881 gctgccgtca gcgcaccggg tggacgtcac cgcttatgcg gcaccggagc aactcatgcc
     2941 cgttgtattt tcgacagagc gaaacagcag gacgctgaat ctgctgttgt gcaaagggcc
     3001 ggaggaaatg cttgtcggtt ttgtgcgcct ggaggacgag ctgcgtcccg ttcttgcgct
     3061 tccgtcgccg gattacagtc atctgatggt aagcatcatc acagagaacg ggatacacct
     3121 ggcaggctac ggtgaggcca taaaccgtga tgctgatact ccgtacccac cggaaccgaa
     3181 gctgatgcag ttcaggctta agggctgtca ggacacgctt tttgctgcca tcaacaaacc
     3241 ggaagagatg ccggattatc tcttccgtca actcggattt aatcagacct ggcatgaatg
     3301 gaagcgggac gaacagcaca ggcaacaaca acgacgcccc ggacattacc ggggcatgag
     3361 tatgtgatat ccggggctgc accccgaacc ccgccaacgt ctcacgggac acgaaaaaat
     3421 tccgcgtccc gtgaggcgtt ttctggatga gaaagtttgc cgttttgacc cgttcgcgct
     3481 tctgaacggt cgaaaccgat cgcgcatcgc tttcgtgaat agttatgcag acccctgaaa
     3541 acgattctga cgcgtttttc tggtttggcc tggtgttttc ttgtcttttt gtgttttttg
     3601 cgtcagaacg cgtctgagag cgttttaagg ggtacgtaca acaggagtta tggtaaatgg
     3661 atcggttttt cgggaaggat cgacaggatt tgccgttggg tgtagtgtaa gcgactgaaa
     3721 aacaaacgcc cctgaaattc gtgtagatcc gccaagactt tcacgaagat acaggggcga
     3781 tgtcggtcga taacctggaa ggaatcgtaa cacatgagcg ccgcgcttca atacttcgaa
     3841 gaaaa
//
