LOCUS       CP077256                4775 bp    DNA     linear   BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1381 plasmid unnamed6.
ACCESSION   CP077256
VERSION     CP077256.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357523
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 886.95x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:20:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,078
            CDSs (total)                      :: 4,960
            Genes (coding)                    :: 4,811
            CDSs (with protein)               :: 4,811
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 68 of 149
            Pseudo Genes (incomplete)         :: 88 of 149
            Pseudo Genes (internal stop)      :: 34 of 149
            Pseudo Genes (multiple problems)  :: 38 of 149
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1381"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1381"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            1766..1975
                     /locus_tag="I6L11_25350"
     CDS             1766..1975
                     /locus_tag="I6L11_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA42723.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(2041..2574)
                     /locus_tag="I6L11_25355"
     CDS             complement(2041..2574)
                     /locus_tag="I6L11_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA42724.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(2577..3503)
                     /locus_tag="I6L11_25360"
     CDS             complement(2577..3503)
                     /locus_tag="I6L11_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QXA42725.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSKQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            3680..4078
                     /locus_tag="I6L11_25365"
     CDS             3680..4078
                     /locus_tag="I6L11_25365"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA42726.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(4282..4680)
                     /locus_tag="I6L11_25370"
     CDS             complement(4282..4680)
                     /locus_tag="I6L11_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="QXA42727.1"
                     /translation="MGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLP
                     VPLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVAT
                     SARTLKPWEALGISRAWYYQLKKRGLVE"
                     /name="primase alpha helix C-terminal domain-containing
                     protein"
     Typing          complement(4283..4653)
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2581..3503)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.727"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
       61 cgacatcgcc cctgtatttt cgtgaaagtc ttggcggata gacacgaatt tcaggggcgt
      121 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagcct tcccgcaaat
      181 ccgatccatt taccataact cccgttgtac gtaccctgta aaacgccctc aatcgctttc
      241 tggcgcaaaa aacgcaaaaa gacaagaaaa caccaggcca aaccagaaaa acgcgtcaga
      301 aacgttttca gggcgttgca taactatgca cgaaagcgat gtgcgatcgg tttcgtgcgt
      361 tctgaacacc gaacggctca aaacgacaag ttttctcccc tggtgcgtgg cgctctgacg
      421 gggtttcctg tttccaaact tcacatctcg ccaggacaaa aaaaaaccga cctggggtcg
      481 gtggcaagaa tcagagtcta tgggaaatat tttagttatc ggatgctcaa tattcacaaa
      541 cgcaaaaaat gcctcagtga tgccctaaaa caacctttcc agaaggagtc cgcgctttac
      601 gatgcacgga agagataaaa aaccccacaa aagtggggaa ccaagagaaa caacaattac
      661 aggttgttgg ttgtgttcga acaacgatga agtagctacg ctaaataata attaccgtac
      721 aggccctaca tattcaacac taaatttaag atcctgacga aggatttata aatgcacgaa
      781 aacacataga cagacaaatt cactaaattc atacacaaaa acaagtgtag ttttctaagt
      841 aaattagatt cgacaaccaa ttagtttatc cacatcgcat tccactcttg ctacaccaat
      901 cacatttctt gatatgtaat agcagaaaaa ataaaaccag acaccaatag accaaaaaac
      961 aaacattcaa caagaaacat tttgaacaat atcacaaaac tgtcacaacc atcatcagac
     1021 tattgaaaaa aactggttgc tgttttataa aagcgactgt aacaacctcc actctatagt
     1081 tccattagtt taaagtgtca cacaaactga gcatccattt gttttcctta cttttgcccc
     1141 tgtattccaa ggggtttttt tataacttaa agattagcac accacacaaa aacaataaaa
     1201 aaaacgacaa gttatacaat aaaaccagta ctatccatac agctaattac atataaatac
     1261 tattaaacca tctggacatt aaaaataaac tcttataaca taaaattaat gcggttccgg
     1321 taccatatgt cagggataat gtgcaaatgg aggtatcgga actgcatttc agttcattat
     1381 taattaatga caaaaaaaac ctctgcgagc gcagaggggg taaggaatgt tcccggtcat
     1441 aatatgtcga tagtgtttat atacatacag aacaagaaca acaaccagcc aactaataaa
     1501 tattttattt tttagagctg aaaccttaaa gctcacttcc attccataca ttgttataaa
     1561 ccttatgttg aaaactgaaa gtaagtacgg cagacttatt aagttgcgtg cagtcttttc
     1621 acgttataca aacaagaaca cctgcgaagt tatttctcct tacatatccc cctatgccaa
     1681 cgggggattt tttctggttc actaacgcat aaataatagt gacaccctat cattcacaaa
     1741 ccgtgataat ataaggatta tcatcatggc aaaattaggt gaaaacaccc cacatattat
     1801 cgatgctact gtagatttta tggcatcaag ccaggcattt cgtgagtatc tgaaaaaact
     1861 tccccctgat aacacaatac cgccagctct tactgatgag cacgtaccgt tatatatacg
     1921 aaggctggaa tactatcgtc tgctttatcg accaaagcag acagaatcat attgaaaaaa
     1981 acggtgaacg gttcgcgaaa tcctttcgcg ggtcgtgagg cgttggacaa gcgaagcgcg
     2041 tcagtttttc cggctcttcc ccgtgacagg tttctccacc agtttatctt ccagtgtcac
     2101 aactttctga tacaccgccg ctgtggcgtt gtagttcctg tcgagtattg ttttcattcc
     2161 ccatccgagc acacagaaca taaccagaaa agccactgac accccaccca gccatttcca
     2221 gctataccct ttaagggtgt tgtattcctg cacgatattg ctgtgaattg tcgcaatatc
     2281 cctgttcagg gcatccgcgt ttgccttcat gtttgcgact gactcttcca gccgctgaaa
     2341 atcctggctg atgcgctcac ctatcagtcc tgccacgtcc agcttctgca cctcatttct
     2401 gacttcatcc ctgacctgtt ttgccacacc gtcagaaata tcccgatatc cgcgaagagt
     2461 ttcccttgcc acagccagag aatcagcaac agctttctgt tgctccattg cggtatgttg
     2521 cagatgctcc accgataaca ggagcgtgag taatttttct tctgtgctgg ccatgttcag
     2581 aatcccatag aaaatccacg tgacggtgat ttaacccgtt caggtgcagt tcggttctgt
     2641 ttgctcactc tgtctctcat gtcaaagcca tgctcccgcg caacatcatg ttgcagcctt
     2701 ctgaccagtt catgttgctt aaccggagtg tccagccatt ccctgatgtc atgcccgacc
     2761 gttcgcgcct gttcaagatg gcgggaatag tccggttcag accgtttttg catgatgtca
     2821 tgacgctggg catcatcaag ccgtccggca gcttcaaaac ccagatgcac ttcgggacgt
     2881 cgttcaatgc cctgttcttt cagcgtccgg cagtcaatga agcccctttc aaagccatgc
     2941 tttctcatat gttcgttgtg cttcacctcc cagcgttttc gcaattcccg caactgctct
     3001 ttttgctgag tgagagtcag gcttccggaa tcctttttcg cacctccccg ctcgggatac
     3061 ctggcgttat agcgccggaa atactgttcc ggtgtacgtt caatgccatc attcacacgc
     3121 tgactcatca tgatatgcgc atgaggctgt tcgccgccgt caatgctcgc tttcggattg
     3181 tgaatggcaa aactccaggc gtgccgttcg ccagcttcct gacgtacaaa atcctgcacc
     3241 agttcaaggc gctgttccgg tgtcagttca cggggtaatg caatttcaag ttccctatag
     3301 gtcgaaccgt tcgcacgttc aaactggtca gccgcctgcc agaaatggga aggattatcc
     3361 ctggcccatt ccggcatgtt tccgtgtgcg gaatattcca aatcctgacg gtcgcggtat
     3421 ttctctttgc gctcgatgta atcggcgtgg tttgcagcct gccccttgcc accaaattta
     3481 acgctgaggt gataggttgc cacggtttca gccctctgca ggagcgcact gtttctcgac
     3541 gaaggagaga aacatataag tgcgcttcct tctaatttta cgaattagca cggaagatac
     3601 cccctgcacc cgccgaccgc aagcgatata ctgcacgacc acaacacacc gcaaaaaaac
     3661 accagggagc atcacgaaca tgcgcatcag caacatcgaa tggctgaaga aacggatcgg
     3721 attcatcagg aagctcggag aacagaccgc acgacagaga cagattattg acctgatcga
     3781 caacgaagcc ggactcacag aacaggaacg caaacttctc cacgtactgg caacagcgga
     3841 aaaaaacgac ttacaggcac aggagagcga acgaaaacag gcggtccaaa aacgaatcga
     3901 gggaaaaaaa caacgacggg aacgcaatca ccggctcttt ctcgcggctg ggctactgat
     3961 cgaagcagga ctggtagaca caaaaacagg cgagctatgc tataaaaaag acaggatatt
     4021 acaggcactt aaggaactta agtacgatct ggagacatcc ccaaacccag acgcgtaagt
     4081 gcctccatcc gtgttccgat tccctggtgc ttcctcgctc attcgctcgc tacgctcggt
     4141 catgagactg cggcgagcac taccaccaca atcacgcgga aagccgcgcc attgcagggc
     4201 tgaacgggga aaaacggcaa aaaacctgct gctcaaaaag acgccagaaa ggcgctgtta
     4261 tctgatatag gcttatttgg tctactctac gagacctcgt tttttcagtt ggtaatacca
     4321 ggcgcgactg atccctaatg cctcccacgg tttcagcgtg cgtgcagatg tagctactgt
     4381 gctgcgctta gacttagaac cgcccttgcg accgcgtgca gcctgaattt ctggcgtgtg
     4441 cgtatcagcc acatactgtg cgaaagtttc cggcgtgaag ttcctgtgcg tgtatttcgc
     4501 aatgctcttg ccaatagccc gacattcagc cagtgaaagc ggaacgggaa gcgatgcatt
     4561 gtacatttcg acacgctgga tcaccgcttc aagccactgt gagaacgctg gccagccctg
     4621 acgaatcgcc ctgtaagccc atttgcgcgt catttcgaac aggtggcagt ttcgccccat
     4681 cccgtaatgt ttatcgatgc tacgacgctc tgaggcgctc aaatcgagat aatcagccag
     4741 ttcatcgagg gtataggctt cctcgcgcca ctcca
//
