LOCUS       CP077255                2102 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1381 plasmid unnamed5, complete
            sequence.
ACCESSION   CP077255
VERSION     CP077255.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357523
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 886.95x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:20:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,078
            CDSs (total)                      :: 4,960
            Genes (coding)                    :: 4,811
            CDSs (with protein)               :: 4,811
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 68 of 149
            Pseudo Genes (incomplete)         :: 88 of 149
            Pseudo Genes (internal stop)      :: 34 of 149
            Pseudo Genes (multiple problems)  :: 38 of 149
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1381"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1381"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            complement(join(1863..2102,1..726))
                     /locus_tag="I6L11_25340"
     CDS             complement(join(1863..2102,1..726))
                     /locus_tag="I6L11_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QXA42722.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            complement(1593..1859)
                     /locus_tag="I6L11_25345"
     CDS             complement(1593..1859)
                     /locus_tag="I6L11_25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA42721.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          complement(102..333)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 ttccggttta acggagtatt tcagcgtttc agcaaccgcg ctttgcagtt gctcggcaac
       61 gttggccaca acctcacctg tcttagtttt tactgcccga atatcgatat tcggctcata
      121 gttcacccgc aagcaatcgc gccagagttc tacccaacgt tcgtgcttaa cgtagttctt
      181 ccctttaaac caagaaggtt gcaccatcag cagacagtga aaatgcggat gtgcgctgcc
      241 atccttacct cgcgtcacct ccgtagccct gatccacccc tgaacaggtg atagctcctt
      301 tcgcttttcc attcgcttaa acgccgcatt cattgctgta aggactgttc caagttcacc
      361 tatctcgcag ttcctgacag ttaacgtcag aaacaaccat cgggaagacg ggtaatccac
      421 aacgattttc ggtagagcct gataaaaacg ggcttgccac atgagggttc ttctccactg
      481 gcagacaggg caatgacgga cacggcaaaa ttgcgcacta cgaagccgta ggcgcgtttc
      541 tccggtttcc atgatggtag accaaccaaa acgtaacaaa ccgctacagg aggccatacg
      601 gagtgcatag cgctcgaatt cagcagcagt ctggtagata cccccgacag aatcggtaag
      661 cgtgcgctgg gtatcccaaa ctgcatcacg ggggctgtag tccgaaagga atttatcttc
      721 tgacattaag cctgctccga tctgggcagg ctgtgatttg atgtggtata ttcatcacgt
      781 acctgtgcat tgcgagccag tacatgtcac cagagtttgc cgctgaggtg acacattcga
      841 aatgttgggg agtgttgacg cactccccaa cgccgattaa aaacgcctca attctgctcc
      901 attttctttg acctgtcaaa acccactcaa agcctcgtca ttactgggct aaaccactat
      961 ttcatatccg ccatttatcc cttaagtata aatacaagga tcacgcgcga gaattgcgca
     1021 aaaaataacc acaattttaa cccccccaaa acaagcaccc ccgaacggct taccccaacc
     1081 ttttccacac acccgatcac caaaacaggg aataaaaaca aaaccctgtc ttacaggtgg
     1141 atggcctcac ttcgtgaaac catccacccc gcataagttt tttaatcggt ggtgtcgtca
     1201 atcactaaat cggaacccca aaggggaccc ccacgattta gcatttgacg agattaactc
     1261 tcggagatgg catcatgctt ttaacggact ttaaaaaacg gtgaacggat gcggatttta
     1321 atccgtgtct gtgaggcgtt gagcaacgag caaagcgagg cggtagaaag agagccagcg
     1381 gcaggctcag gacgataatc cggggaaaat gtaccactcg cagagtgtta acatttgaca
     1441 tcggattgtc tatcctgcat tggtgtcgag ttggcaaggc tgaggataat gctgaggata
     1501 atgctgagga taatgcagga tagacagagc actagagatt gcatgcaatc tcgtacctat
     1561 cacctcagcc tgccgctggc tcttttccag aactaagaat gcgttcttaa aaatccaaac
     1621 ttgataccgt ttaaatctat tttcacaaaa agagttttta ccctctcgtt tgttttctga
     1681 atatataaaa ttaattcatc ctcaaaacct tcgtgcggat gaaaaccaaa tgtgccaaaa
     1741 cttaatccat caataagcaa aataaggttt ttaaacctgt caggaatcac cgcttcactt
     1801 cttaaaaaaa ataaaccaat agtcttattt ccattcaggt attcaatatt aatattcata
     1861 cgttaatccg atttatcctt ctcaggggcg cgtttgtaac gccgtttacc tgaatcccag
     1921 acaaacgccg tccgcttccc gtcatcagtg ccatccccta catcgtcggc aatgacaaga
     1981 tcctcattgg tttctcgatc caactggagg acgtttttta gcgccccacc ggtcgagata
     2041 aaacggcgct tgtgaagctg ccgcgtcagc tcaagaaacc actcaggatc gtttgccata
     2101 tc
//
