LOCUS       CP077253                4082 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1381 plasmid unnamed3, complete
            sequence.
ACCESSION   CP077253
VERSION     CP077253.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357523
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 886.95x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:20:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,078
            CDSs (total)                      :: 4,960
            Genes (coding)                    :: 4,811
            CDSs (with protein)               :: 4,811
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 68 of 149
            Pseudo Genes (incomplete)         :: 88 of 149
            Pseudo Genes (internal stop)      :: 34 of 149
            Pseudo Genes (multiple problems)  :: 38 of 149
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1381"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1381"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            complement(join(3275..4082,1..1352))
                     /locus_tag="I6L11_25290"
     CDS             complement(join(3275..4082,1..1352))
                     /locus_tag="I6L11_25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QXA42713.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCMNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            1531..1785
                     /locus_tag="I6L11_25295"
     CDS             1531..1785
                     /locus_tag="I6L11_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QXA42714.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     gene            2318..3019
                     /locus_tag="I6L11_25300"
     CDS             2318..3019
                     /locus_tag="I6L11_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QXA42712.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(995..1353)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.722"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1354..1530
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          2319..3019
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..1439)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.582"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3279..4081)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atgggggcct cgccataccc cgtaacgact gcgctcagta cccctgaatc cataacggca
       61 ttgtcatttt tcctgagctg ttccgctcgt cgctccgcgc gactacgttc tgtatcctcc
      121 tgctccttcc ggcgcaattc ctcttcacgt ttaagcaact ggactctgag agctgcaagg
      181 ccggatgccc cccactgctg ccactgtttt tcaaatgccg tctgactcgc gggaatgtcc
      241 tgtttctcca ccttccggta atgctgtcgc aatttttcca tttgctggct cagcgactgg
      301 atttttccct gttcgctacg gtattccggt gccgcccatt gcatcagggg aatacgggaa
      361 aaaccggacg aaaacggcga tgattttttc aggctggcag catggctcat cgcttccttt
      421 cgttcggtgt tcagctcttc ccagcgtctg cgcgtcgcct gcatatctgc catcatcgac
      481 atggcattac tggtgagttt ccatgccgca ttactgtcct tcggcaacgc ttcgatgatg
      541 tcacgtagtg tgtacgaagt tgcgttgtca ggaagcacga tattcagaag gtcagccaca
      601 cggcgggcag gctctgtcag cccgctgttt ctgagttcgg tacgggaaat ctgtatttcc
      661 aggttaagtc tgttaatcag acgattctgt tcgccgcgtt ctgtttcgat accgcgtgtt
      721 tccatcgcac aggcagactt tccgaggtga ataccaggcg ttttctcaag cccctgtcgc
      781 tcgtatgaac gatggtctat ctcttcctga taaccggcgt tcgccagtgc cctgttcgca
      841 tggtcagccc atgatgcacg ccacgtctcg gcatgtgtcc ggtcgttcca gtccctgacc
      901 tttccaccga atccagcagg tccgacagct ctcgtggtca gcatgatgtg cgcatgggga
      961 tttgttttat ccatgtgatg gaacgccaca tcagcaatca tgccctgact gacaaagttt
     1021 tctctgacaa aggcgagaac ggtttcccgt gcggcatcct gcggtagttc gcgggggatg
     1081 gcgagttcaa tttcccgcgc aagttgtgag ttcttccggc gttcggcctt ctcaacggca
     1141 ttccagagtt cggcacggtt catacaccag gcaggggctg tcgcgggagt cagaatcaca
     1201 gcttcagcca caccgctttt gcgggtgtag tcgtgagtca gtccggtacg ctcatcaagc
     1261 atcagtgaac cggaacgata ggcagcagaa gcaacggcgc tgtagccgct gtttctggag
     1321 atgattttca tgctgagatg ataaatcgcc atttctgccg taatgccttt ttgtggtttt
     1381 ctttcagccc cgcagggcgc acacaatcga aacggagtgg agattgtata agtgcgcact
     1441 tcgtgatttt ccatgcatta tgccagctca gtttttaaac taccagtgac gcgctaaaat
     1501 gcgatttttc atcgcgcacg gagttacatc atgagcagaa cactcgaaca gaagattgct
     1561 gatgctgagg ccagattaca gcgcctgaag gcgaagagca ggagtctgga cacagcgcag
     1621 aaggtcattg tgggtgcagc attgctggca aaggtcagaa agccggagga ggtgcagttg
     1681 cgtgcctggt tactccagtt cctgaaggca gaggtgacac gacaggctga tgtgacgcgc
     1741 atactgccgc tgattaacga gctggaagcg ctgccaggac agtgattgta tcgtccgctc
     1801 gccgcagtcc cagcgaccga gcgttagcga gcgagtgggc gaggaagcgg aagtgaatcc
     1861 gtcgcctgat tgagcacata cgcacggttt ttcgggaggt gatatgcgct gaacccgcat
     1921 gaatactgga tttccacagg gttggagggg gtgtattcgg aacttgcaat tctgcttttt
     1981 tgcaacgcat tgtttttatt ggttttttgt tttcagcact ccaagttatc cacaggctcg
     2041 tcgttaagtt tttagatctt gtttttaaga tcttgttttt aatacttgtt ttagcgccaa
     2101 aattccttat agaaaacaaa atgttacaac gctataggta atatgattta aggtaaaggt
     2161 aatatgattt aaggtgttta ggtggaggaa tttaaggtaa aggcaatatg atttaaggta
     2221 atattcacag aaaaagggat attaaaataa aaaggcattg cctttcgttg agggtatgtt
     2281 tatcttttat tcattacctt ttggggaggg atgctctatg tcggatctaa ttgcctataa
     2341 atccaatgcg ctggttgaag ccagttataa attgacccta caggaacagc gatttttgtt
     2401 actttgcatc ggtcgattga agtctggggc tgatgcagaa tctccaaaat tgcaaaagac
     2461 aatgaccatt acagctgcgg aatatttcga ttcattccct gacatggggc ggaaaaatgc
     2521 cgaggttcag ttacaggaag caattgatcg tttgtgggac aggtcaatca ttctcaagga
     2581 tgacgagaaa cgtgaggagt tccgctggat ccagtatcgg gcacagtacg ctaaaggcga
     2641 ggccagagca caaataacat tttctgacgc agtaatgcct tatttgacac aactacaagg
     2701 gcaatttact agagttgtaa tcaagaatat atctagatta tcaagaagtt acagtataag
     2761 gatatacgaa ctcctccagc agttccgcag tactggcgaa cgtattatcg ctctcgatga
     2821 ttttcgctca atgcttgata tagagcataa atatcaaaca tataaatcac ttaaccagca
     2881 attattaaga ccctgcattg acgaactgaa caaaaaaagc gacctggctg taacggttga
     2941 gacgatcaaa aaggggcgca cggttgtagc cctgcatttt cgcttcaagg aagacaaaca
     3001 aatcaagatg acgatctaaa atcgatttta aggcacctaa aacgctccct gctgcgtttt
     3061 ttttgtgtta gtagtgcgaa ggtactattg aagtgaaaaa ttgcgtcaaa agcgctctaa
     3121 tgcagcgaaa aaagaacccc cattttttgg gggcggaaaa acaaaactat gcttgcatta
     3181 tgtgtgtgct tcttgcattc atccctgtcg atgaacatcg aagaacatcg acgaacataa
     3241 cacgatgtta aacaaaacaa aatattgttc acattcactg tgaccaaccg cgatcatgtc
     3301 tcctctcggt ggcgtgtgtt cgtgcatctt cctcgcgccg ccattcgtcc caggtacgat
     3361 taaaacccag cgagcggaac agatgatccg gctgtttttc tggtgtgttg acggcagcaa
     3421 aaagcggtgc ctcacgggtt ttcagatgaa actccataag ttccggcgca ggtgatatgt
     3481 ggctgtctgc atcgtgattt acggcaacac cataccctgc ataccgtaac cccttatcgg
     3541 tcagtgcact gatgaccagc tggctgttat cggctgaggg aacggcaaac gcgggataca
     3601 gcgtaccatt aagccggaca acaccgccca gagagtcatc cacgcccatg accagcaaaa
     3661 gctgaagctg gcgggtttcc cggtcgacag aactcattac cggcatcagc gtcggtagca
     3721 gacgtccctg cgtaaaatcc accttccttg ctgccagtga tggcatggct ggctggcggt
     3781 cttctccaag acagaaagac tccggccatg tcttcggctg gcggagattg cgggtatatt
     3841 gcggccatgt ctggcaaacc tgcgtcagaa gttgctcaaa ggtcgcagag gaataaacac
     3901 gttgcagtgg tggatgttga ccgatttcgc ttcggtcaca gatataacca ctcctgtcac
     3961 gcattaaatc aacacgctca aagattctgg tgcggtattt ctccagctcg ttcccccaga
     4021 gcgtgtattc tccgttccgg ttgtgaagca gaaggtaaca ggtcatttcc ccgttaccag
     4081 gc
//
